LOCUS       40924_26451        3035 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pK18mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Tauch A, Zheng Z, Puhler A, Kalinowski J.
  TITLE     Corynebacterium striatum chloramphenicol resistance transposon 
            Tn5564: genetic organization and transposition in Corynebacterium 
            glutamicum
  JOURNAL   Plasmid 40 (2), 126-139 (1998)
  PUBMED    9735314
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   3  (bases 1 to 3035)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   4  (bases 1 to 3035)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3035)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "1998"; volume: "40"; issue: "2"; pages: "126-139"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(61..77)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             584..1375
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     oriT            complement(1716..1825)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2037..2625
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2913..2934
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2949..2979
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2987..3003
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3011..3027
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gataagctag cttcacgctg
      241 ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc
      301 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
      361 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
      421 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt
      481 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag
      541 gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
      601 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
      661 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
      721 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctccaagacg aggcagcgcg
      781 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
      841 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
      901 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
      961 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     1021 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     1081 gccagccgaa ctgttcgcca ggctcaaggc gcggatgccc gacggcgagg atctcgtcgt
     1141 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     1201 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     1261 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     1321 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     1381 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     1441 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccctc
     1501 gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga cggccagcag
     1561 gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc
     1621 tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg tttcatcagc
     1681 catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt
     1741 cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg
     1801 tgggcctact tcacctatcc tgccccgctg acgccgttgg atacaccaag gaaagtctac
     1861 acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat
     1921 cgctataatg accccgaagc agggttatgc agcggaaaag ccatgaccaa aatcccttaa
     1981 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2041 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2101 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2161 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     2221 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2281 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2341 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2401 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     2461 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2521 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2581 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2641 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2701 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2761 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     2821 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     2881 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2941 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     3001 caatttcaca caggaaacag ctatgacatg attac
//
