LOCUS       40924_26471        3053 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pK18PolyF2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J.
  TITLE     Efficient electrotransformation of corynebacterium diphtheriae with 
            a mini-replicon derived from the Corynebacterium glutamicum plasmid 
            pGA1
  JOURNAL   Curr. Microbiol. 45 (5), 362-367 (2002)
  PUBMED    12232668
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 3053)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3053)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Microbiol."; date: "2002"; volume: "45"; issue: "5"; pages: 
            "362-367"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..95)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             602..1393
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     oriT            complement(1734..1843)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2055..2643
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2931..2952
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2967..2997
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3005..3021
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3029..3045
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgtta acgaattctg cagcgctgca ggatccccgg ggatcctcta gatatctaga
       61 agcttttaaa agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      121 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      181 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga
      241 taagctagct tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa
      301 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
      361 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
      421 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
      481 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
      541 aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg
      601 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
      661 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
      721 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
      781 ccaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
      841 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
      901 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
      961 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     1021 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     1081 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc ggatgcccga
     1141 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     1201 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     1261 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     1321 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     1381 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     1441 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     1501 gttttccggg acgccctcgc ggacgtgctc atagtccacg acgcccgtga ttttgtagcc
     1561 ctggccgacg gccagcaggt aggccgacag gctcatgccg gccgccgccg ccttttcctc
     1621 aatcgctctt cgttcgtctg gaaggcagta caccttgata ggtgggctgc ccttcctggt
     1681 tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca
     1741 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     1801 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg ccccgctgac gccgttggat
     1861 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     1921 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaagcc
     1981 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2041 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2101 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2161 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     2221 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2281 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2341 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2401 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     2461 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2521 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2581 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2641 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2701 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2761 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2821 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2881 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     2941 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     3001 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tac
//
