LOCUS       V005271                 4589 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005271
VERSION     V005271
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Novel sulI binary vectors enable an inexpensive foliar selection
            method in Arabidopsis
  JOURNAL   BMC Res Notes 4 (1), 44 (2011)
   PUBMED   21366919
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Crop Improvement and Utilization Research
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 4589)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4589)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res
            Notes"; date: "2011"; volume: "4"; issue: "1"; pages: "44"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-NOV-2010) Crop Improvement and Utilization Research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(21..180)
                     /label="CaMV35S polyA terminator"
                     /note="CaMV35S polyA terminator"
                     /regulatory_class="terminator"
     CDS             complement(239..1075)
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     transit_peptide complement(1076..1177)
                     /gene="Sul1"
                     /note="pea rbcS ribulose-bisphosphate carboxylase small
                     subunit transit peptide for plastid targeting"
     promoter        complement(1184..1855)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     primer_bind     complement(1963..1979)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1987..2003)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2011..2041)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2056..2077)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2365..2953)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3127..3984)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3985..4089)
                     /label="AmpR promoter"
     primer_bind     4570..4586
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cactggattt tggttttagg aattagaaat tttattgata
       61 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
      121 aaaccctata agaaccctaa ttcccttatc tgggaactac tcacacatta ttctggagaa
      181 aaatagagag agatagattt gtagagagag actggtgatt tttgcggact ctagactagg
      241 catgatctaa ccctcggtct ctggcgtcgc gactgcgaaa tttcgcgagg gtttccgaga
      301 tggtgattgc gcttcgcaga tctccaggcg cgtgggtgcg gacgtagtca gcgccattgc
      361 cgatcgcgtg aagttccgcc gcaaggctcg ctggacccag atcctttaca ggaaggccaa
      421 cggtggcgcc caagaaggat ttccgcgaca ccgagaccaa tagcggaagc cccaacgccg
      481 acttcagctt ttgaaggttc gacagcacgt gcagcgatgt ttccggtgcg gggctcaaga
      541 aaaatcccat ccccggatcg aggattagcc ggtcggcagc gaccccgctc cgtcgcaagg
      601 cggaaacccg cgcctcgaag aaccgcacaa tctcgtcgag cgcgtcttcg ggtcgaaggt
      661 gaccggtgcg ggtggcgatg ccatcccgct gcgctgagtg cataaccacc agcctgcagt
      721 ccgcctcagc aatatcggga tagagcgcag ggtcaggaaa tccttggata tcgttcaggt
      781 agcccacgcc gcgcttgagc gcatagcgct gggtttccgg ttggaagctg tcgattgaaa
      841 cacggtgcat ctgatcggac agggcgtcta agagcggcgc aatacgtctg atctcatcgg
      901 ccggcgatac aggcctcgcg tccggatggc tggcggccgg tccgacatcc acgacgtctg
      961 atccgactcg cagcatttcg atcgccgcgg tgacagcgcc ggcggggtct agccgccggc
     1021 tctcatcgaa gaaggagtcc tcggtgagat tcagaatgcc gaacaccgtc accatgcact
     1081 ttactcttcc accattgctt gtaatggaag taatgtcagt gttgaccttc ttaactggga
     1141 atccagtcat ggatttgagg ccgccgaatg ggcccatctc gaggtcctct ccaaatgaaa
     1201 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
     1261 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     1321 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     1381 atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc caccttcctt
     1441 ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga ggaggtttcc
     1501 ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga ctgtatcttt
     1561 gatatttttg gagtagacaa gtgtgtcgtg ctccaccatg ttatcacatc aatccacttg
     1621 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     1681 atctttggga ccactgtcgg cagaggcatc ttcaacgatg gcctttcctt tatcgcaatg
     1741 atggcatttg taggagccac cttccttttc cactatcttc acaataaagt gacagatagc
     1801 tgggcaatgg aatccgagga ggtttccgga tattaccctt tgttgaaaag tctcaattgc
     1861 cctttggtct tctgagactg tatctttgat atttttggag tagacaagtg tgtcgtgctc
     1921 caccatgttg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt
     1981 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2041 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2101 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2161 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2221 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2281 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2341 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2401 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2461 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2521 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2581 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2641 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2701 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2761 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     2821 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2881 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2941 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3001 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3061 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3121 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3181 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3241 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3301 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3361 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3421 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3481 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3541 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3601 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3661 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3721 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3781 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3841 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3901 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3961 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4021 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4081 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     4141 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctttcgt ctcgcgcgtt
     4201 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     4261 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     4321 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     4381 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     4441 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     4501 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     4561 cacgacgttg taaaacgacg gccagtgcc
//
