LOCUS       62056_13120        4944 bp DNA     circular SYN 09-OCT-2019
DEFINITION  Cloning vector pJexpress412, complete sequence.
ACCESSION   MK689178
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Faber BW.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2019) Parasitology, Biomedical Primate Research 
            Center, Lange Kleiweg 161, Rijswijk, zuid holland 2288 GJ, The 
            Netherlands
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-MAR-2019) Parasitology, Biomedical Primate Research Center, 
            Lange Kleiweg 161, Rijswijk, zuid holland 2288 GJ, The Netherlands"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      86..674
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     944..960
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1424..1442
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1443..1467
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2812..2829
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             2839..2856
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2870..2917
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3339..3386
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             3405..3776
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             3862..4941
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc
       61 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
      121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
      241 tgtagccgta gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      721 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      781 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      841 gcgaggaagc ggaaggcgag agtagggaac tgccaggcat caaactaagc agaaggcccc
      901 tgacggatgg cctttttgcg tttctacaaa ctctttctgt gttgtaaaac gacggccagt
      961 cttaagctcg ggccccctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg
     1021 taaaaacccg cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag
     1081 aatatttaag ggcgcctgtc actttgcttg atatatgaga attatttaac cttataaatg
     1141 agaaaaaagc aacgcacttt aaataagata cgttgctttt tcgattgatg aacacctata
     1201 attaaactat tcatctatta tttatgattt tttgtatata caatatttct agtttgttaa
     1261 agagaattaa gaaaataaat ctcgaaaata ataaagggaa aatcagtttt tgatatcaaa
     1321 attatacatg tcaacgataa tacaaaatat aatacaaact ataagatgtt atcagtattt
     1381 attatgcatt tagaataaat tttgtgtcgc ccttccgcga aattaatacg actcactata
     1441 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa cttttaggag
     1501 ataaaacata tgtttacgat catggccgca atcctggctt acaccatcgg tactacccac
     1561 ttccagcgtg cactgatctt catcctgctg actgctgttg ctccatctat gactatgcgt
     1621 tgcgttggta tcggttcccg tgacttcgtt gaaggtctgt ccggtgcaac ttgggttgat
     1681 gttgtcctgg aacacggttc ttgtgttacc actatggcca aggacaagcc aactctggac
     1741 atcgagctgc tgaagactga agtgactaac ccagcagtcc tgcgtaagct gtgtatcgaa
     1801 gcaaagatct ccaacaccac gacggactcc cgttgtccta ctcagggtga agcaactctg
     1861 gttgaagaac aggacgcaaa cttcgtgtgt cgtcgtactt tcgtggaccg tggttggggt
     1921 aatggttgtg gtctgttcgg taagggttct ctgatcactt gcgctaagtt caaatgcgtg
     1981 acgaaactgg agggtaaaat cgtccagtac gagaacctga aatactccgt gatcgtgacc
     2041 gtgcacaccg gcgaccagca ccaggttggt aacgaatcca ctgagcacgg taccactgct
     2101 actatcaccc cacaggctcc tactactgaa atccagctga ctgactacgg tgcactgact
     2161 ctggactgct ctcctcgtac cggtctggat tttaacgaaa tggtgctgct gacgatgaaa
     2221 gagaaatcct ggctggtgca caaacagtgg ttcctggacc tgcctctgcc ttggacctct
     2281 ggtgcatcta cgtctcaaga aacctggaat cgtcaagatc tgctggttac cttcaaaacc
     2341 gcacacgcaa aaaaacagga ggtggtggtc ctgggttccc aggaaggtgc tatgcatacc
     2401 gctctgaccg gtgctaccga aattcaaacc tctggtacca cgaccatttt cgcaggtcat
     2461 ctgaaatgcc gtctgaaaat ggataaactg accctgaaag gcatgtccta cgtcatgtgc
     2521 accggctcct tcaaactgga gaaagaagtc gccgaaaccc agcacggtac cgtactggta
     2581 cagatcaaat atgaaggcac cgatgcccca tgcaaaatcc ctttctccac ccaggatgaa
     2641 aaaggcgtta cccagaacgg ccgtctgatc accgcaaacc caatcgtaac cgataaagaa
     2701 aaaccagtaa acatcgaagc tgaaccacct ttcggcgaat cctacatcgt aattggcgcc
     2761 ggtgaaaaag ccctaaaact gtcgtggttc aaaaaaggcg gcggcggcgg acaccatcat
     2821 caccatcact aagtcgacca tcatcatcac caccactaac tcgagccccc tagcataacc
     2881 ccttggggcc tctaaacggg tcttgagggg ttttttgccc ctgagacgcg tcaatcgagt
     2941 tcgtacctaa gggcgacacc ccctaattag cccgggcgaa aggcccagtc tttcgactga
     3001 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcatggggag tccccacact
     3061 accatcggcg ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc
     3121 gctactgccg ccaggcaaac aaggggtgtt atgagccata ttcaggtata aatgggctcg
     3181 cgataatgtg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3241 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg
     3301 taactcacgt taagggattt tggtcatgat cagcacgtgt tgacaattaa tcatcggcat
     3361 agtatatcgg catagtataa tacgacaagg tgaggaacta aaacatggcc aagttgacca
     3421 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     3481 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     3541 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     3601 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     3661 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     3721 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgag
     3781 cggcgcgcca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag
     3841 agtcaattca gggtggtgaa tatgaaacca gtaacgttat acgatgtcgc agagtatgcc
     3901 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
     3961 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
     4021 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
     4081 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
     4141 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
     4201 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
     4261 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
     4321 acacccatca acagtattat tttctcccat gaggacggta cgcgactggg cgtggagcat
     4381 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
     4441 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
     4501 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
     4561 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
     4621 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
     4681 gacgataccg aagatagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
     4741 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     4801 aagggcaatc agctgttgcc agtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     4861 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     4921 tcccgactgg aaagcgggca gtga
//
