LOCUS       40924_25886        9019 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pJawohl8-RNAi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich IE.
  TITLE     pJawohl8-RNAi a binary vector for gene silencing in plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich IE.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2001) Biochemistry, Max-Planck-Institut 
            f.Zuechtungsforschung, Carl-von-Linne Weg 10, Koeln, NRW D-50829, 
            Germany
REFERENCE   3  (bases 1 to 9019)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9019)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2001) Biochemistry, Max-Planck-Institut 
            f.Zuechtungsforschung, Carl-von-Linne Weg 10, Koeln, NRW D-50829, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    29..153
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        178..208
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             262..918
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1263..1565
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(1609..1733)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    1762..2048
                     /note="contains intron 1 of Arabidopsis thaliana WRKY 
                     transcription factor 33"
     protein_bind    2076..2200
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2244..2546)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(2891..3547)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3601..3631)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(3656..3780)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4097..4121
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      4233..4942
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5016..5107
                     /label=AmpR promoter
     CDS             5108..5965
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6139..6727
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7039..7063
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(7157..7409)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7432..7980)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(8023..8202)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        8346..9019
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 ctcgagaaag aggatccacc tgaggatcac aagtttgtac aaaaaagctg aacgagaaac
       61 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
      121 atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt
      181 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt
      241 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
      301 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
      361 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
      421 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
      481 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
      541 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      601 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      661 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      721 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      781 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      841 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      901 gagtggcagg gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat
      961 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     1021 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     1081 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     1141 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     1201 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     1261 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     1321 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     1381 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     1441 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     1501 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     1561 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     1621 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     1681 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatcctc
     1741 cgttccatgg gctagaagct tctcctcctc tgctaacgta agcctctctg ttttttttct
     1801 ctgtttcttt tgaaatgaat ccaattagtg atgataatct gtgtttgatg tatcattgat
     1861 ttaacatctt gacaatgaat cgtgatcgga agtgataaag ttatgggtca acggtttcaa
     1921 agagagagaa agacttttag agtcaactct cgactctttc ttaattatgt tattgctatt
     1981 tgtctctttt cttgaagtct gaacaattct tgggattgtt ttgcaggttc tagcttctcc
     2041 aaccacagga attcatcgcc cgggactggg ttatcaccac tttgtacaag aaagctgaac
     2101 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
     2161 ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc agactggctg
     2221 tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg
     2281 tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac cggcacactg
     2341 gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt
     2401 tcacgggaga ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc
     2461 ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt
     2521 ttataggtgt aaaccttaaa ctgcatttca ccagtccctg ttctcgtcag caaaagagcc
     2581 gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg
     2641 ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccagac cggagatatt
     2701 gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc
     2761 tgcttcatag cacacctctt tttgacatac ttcgggtata catatcagta tatattctta
     2821 taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc
     2881 cacgcgttta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     2941 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
     3001 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     3061 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     3121 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     3181 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     3241 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     3301 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa
     3361 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     3421 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     3481 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     3541 tctccatttt agcttcctta gctcctgaaa atctcgacgg atcctaactc aaaatccaca
     3601 cattatacga gccggaagca taaagtgtaa agcctggggt gcctaatgcg gccgccatag
     3661 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
     3721 taatatattg atatttatat cattttacgt ttctcgttca gcttttttgt acaaacttgt
     3781 gataacttcc gatcgattct agactagttc tagagtccgc aaaaatcacc agtctctctc
     3841 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     3901 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3961 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     4021 gtgaccgggc ggccgccacc gcggtggagg gggatcagat tgtcgtttcc cgccttcagt
     4081 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     4141 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     4201 gtgtacatca ccgacgagca aggcaagacc gagcgccttt ccgacgctca ccgggctggt
     4261 tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc
     4321 gaagccgtgt gcgagacacc ggccgccggc gttgtggata cctcgcggaa aacttggccc
     4381 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt
     4441 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa
     4501 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga
     4561 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat
     4621 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg
     4681 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc
     4741 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg
     4801 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc
     4861 tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac
     4921 ggcctcaccc caaaaatggc agcgctggca gtccttgcca ttgccgggat cggggcagta
     4981 acgggatggg cgatcagccc gacaagctac ccctatttgt ttatttttct aaatacattc
     5041 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5101 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5161 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5221 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5281 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5341 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5401 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5461 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5521 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5581 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5641 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5701 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5761 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5821 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5881 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5941 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6001 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6061 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6121 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6181 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6241 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6301 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6361 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6421 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6481 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6541 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6601 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6661 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6721 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6781 tcacatggac tctagctaga ggatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     6841 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     6901 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     6961 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     7021 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     7081 acttaataac acattgcgga cgtttttaat gatcgaatac taacgtctct accagatatc
     7141 agcttgcatg ccggtcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     7201 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     7261 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     7321 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     7381 aaactttatt gccaaatgtt tgaacgatct gcttgactct aggggtcatc agatttcggt
     7441 gacgggcagg accggacggg gcggcaccgg caggctgaag tccagctgcc agaaacccac
     7501 gtcatgccag ttcccgtgct tgaagccggc cgcccgcagc atgccgcggg gggcatatcc
     7561 gagcgcctcg tgcatgcgca cgctcgggtc gttgggcagc ccgatgacag cgaccacgct
     7621 cttgaagccc tgtgcctcca gggacttcag caggtgggtg tagagcgtgg agcccagtcc
     7681 cgtccgctgg tggcgggggg agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt
     7741 gcgtgccttc caggggcccg cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac
     7801 gagccaggga tagcgctccc gcagacggac gaggtcgtcc gtccactcct gcggttcctg
     7861 cggctcggta cggaagttga ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac
     7921 cgccggcatg tccgcctcgg tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat
     7981 ggtagatccc cctcgatcga gttgagagtg aatatgagac tctaattgga taccgagggg
     8041 aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc
     8101 ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact
     8161 ccagaaaccc gcggctcagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa
     8221 aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca
     8281 tgatcagatt gtcgtttccc gccttcggtt tgggcgcgcc cggtctcaga agaccagagg
     8341 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
     8401 gctatctgtc acttcatcga aaggacagta gaaaaggaag atggcttcta caaatgccat
     8461 cattgcgata aaggaaaggc tatcgttcaa gaatgcctct accgacagtg gtcccaaaga
     8521 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     8581 gcaagtggat tgatgtgata acatggtgga gcacgacact ctcgtctact ccaagaatat
     8641 caaagataca gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc
     8701 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     8761 aaaggaagat ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     8821 atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa
     8881 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     8941 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     9001 catttcattt ggagaggac
//
