LOCUS       40924_25881        5597 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pJawohl3-RNAi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich I.
  TITLE     pJawohl3-RNAi, a binary vector for gene silencing in plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich I.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2001) Biochemistry, Max-Planck-Institut f. 
            Zuechtungsforschung, Carl-von-Linne Weg 10, Koeln, NRW D-50829, 
            Germany
REFERENCE   3  (bases 1 to 5597)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5597)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-AUG-2001) Biochemistry, Max-Planck-Institut f. 
            Zuechtungsforschung, Carl-von-Linne Weg 10, Koeln, NRW D-50829, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..50
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    51..337
                     /note="Arabidopsis thaliana putative WRKY-type DNA-binding 
                     protein (transcription factor) intron presented in Genbank 
                     Accession Number AC005499"
     misc_feature    338..386
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    387..604
                     /note="Cauliflower mosaic virus 35S polyadenylation signal"
     misc_feature    675..699
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      811..1520
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1594..1685
                     /label=AmpR promoter
     CDS             1686..2543
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2717..3305
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3617..3641
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3735..3987)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4010..4558)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(4601..4780)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4924..5597
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 ctcgagaaag aggatccacc tgaggcctcc gttccatggg ctagaagctt ctcctcctct
       61 gctaacgtaa gcctctctgt tttttttctc tgtttctttt gaaatgaatc caattagtga
      121 tgataatctg tgtttgatgt atcattgatt taacatcttg acaatgaatc gtgatcggaa
      181 gtgataaagt tatgggtcaa cggtttcaaa gagagagaaa gacttttaga gtcaactctc
      241 gactctttct taattatgtt attgctattt gtctcttttc ttgaagtctg aacaattctt
      301 gggattgttt tgcaggttct agcttctcca accacaggaa ttcatcgccc gggactgggt
      361 taacttccga tcgattctag actagttcta gagtccgcaa aaatcaccag tctctctcta
      421 caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga
      481 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
      541 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
      601 gaccgggcgg ccgccaccgc ggtggagggg gatcagattg tcgtttcccg ccttcagttt
      661 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
      721 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
      781 gtacatcacc gacgagcaag gcaagaccga gcgcctttcc gacgctcacc gggctggttg
      841 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
      901 agccgtgtgc gagacaccgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
      961 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
     1021 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
     1081 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
     1141 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
     1201 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
     1261 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
     1321 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
     1381 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
     1441 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
     1501 cctcacccca aaaatggcag cgctggcagt ccttgccatt gccgggatcg gggcagtaac
     1561 gggatgggcg atcagcccga caagctaccc ctatttgttt atttttctaa atacattcaa
     1621 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1681 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1741 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1801 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1861 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1921 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1981 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2041 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2101 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2161 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2221 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2281 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2341 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2401 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2461 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2521 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     2581 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     2641 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2701 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2761 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2821 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2881 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2941 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3001 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3061 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3121 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3181 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3241 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3301 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3361 acatggactc tagctagagg atcacaggca gcaacgctct gtcatcgtta caatcaacat
     3421 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
     3481 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
     3541 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
     3601 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
     3661 ttaataacac attgcggacg tttttaatga tcgaatacta acgtctctac cagatatcag
     3721 cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
     3781 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
     3841 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
     3901 attcaacaga aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa
     3961 actttattgc caaatgtttg aacgatctgc ttgactctag gggtcatcag atttcggtga
     4021 cgggcaggac cggacggggc ggcaccggca ggctgaagtc cagctgccag aaacccacgt
     4081 catgccagtt cccgtgcttg aagccggccg cccgcagcat gccgcggggg gcatatccga
     4141 gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc gatgacagcg accacgctct
     4201 tgaagccctg tgcctccagg gacttcagca ggtgggtgta gagcgtggag cccagtcccg
     4261 tccgctggtg gcggggggag acgtacacgg tcgactcggc cgtccagtcg taggcgttgc
     4321 gtgccttcca ggggcccgcg taggcgatgc cggcgacctc gccgtccacc tcggcgacga
     4381 gccagggata gcgctcccgc agacggacga ggtcgtccgt ccactcctgc ggttcctgcg
     4441 gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg
     4501 ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg gcgtcgttct gggctcatgg
     4561 tagatccccc tcgatcgagt tgagagtgaa tatgagactc taattggata ccgaggggaa
     4621 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
     4681 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     4741 agaaacccgc ggctcagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     4801 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     4861 atcagattgt cgtttcccgc cttcggtttg ggcgcgcccg gtctcagaag accagagggc
     4921 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
     4981 tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca
     5041 ttgcgataaa ggaaaggcta tcgttcaaga atgcctctac cgacagtggt cccaaagatg
     5101 gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     5161 aagtggattg atgtgataac atggtggagc acgacactct cgtctactcc aagaatatca
     5221 aagatacagt ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg
     5281 gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa
     5341 aggaagatgg cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagaat
     5401 gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa
     5461 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
     5521 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
     5581 tttcatttgg agaggac
//
