LOCUS       40924_26436       11951 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pJZ253, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11951)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Pest control. Full crop protection from an insect pest by expression
            of long double-stranded RNAs in plastids
  JOURNAL   Science 347 (6225), 991-994 (2015)
  PUBMED    25722411
REFERENCE   2  (bases 1 to 11951)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) Bock, Max Planck Institute of Molecular 
            Plant Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476, 
            Germany
REFERENCE   3  (bases 1 to 11951)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11951)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2015"; volume: "347"; issue: "6225"; pages: "991-994"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-DEC-2014) Bock, Max Planck Institute of Molecular Plant 
            Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1057..1402
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1436..1732
                     /label=similar to Leptinotarsa decemlineata actin gene
                     /note="similar to Leptinotarsa decemlineata actin gene"
     intron          1740..1940
                     /gene="St-GA20 ox"
                     /number=1
                     /note="first intron from potato GA20 oxidase gene"
     gene            1740..1940
                     /gene="St-GA20 ox"
                     /label=St-GA20 ox
                     /note="GA20 oxidase"
     misc_feature    complement(1948..2244)
                     /label=similar to Leptinotarsa decemlineata actin gene
                     /note="similar to Leptinotarsa decemlineata actin gene"
     terminator      2254..2961
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(3002..3020)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3053..3069)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3272..3296
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4596..5222
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5659..6723
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6792..6986
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7330..7470
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7656..8244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8334..9125)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(9328..9542)
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     misc_feature    9550..9574
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9652..9826)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9869..10891)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(10959..11636)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11827..11848
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11863..11893
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11901..11917
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11925..11941
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aatttggcca agtcggcctc taatacgact cactataggg agctcgtcga gcggccgctc
       61 gacgaattaa ttccaatccc acaaaaatct gagcttaaca gcacagttgc tcctctcaga
      121 gcagaatcgg gtattcaaca ccctcatatc aactactacg ttgtgtataa cggtccacat
      181 gccggtatat acgatgactg gggttgtaca aaggcggcaa caaacggcgt tcccggagtt
      241 gcacacaaga aatttgccac tattacagag gcaagagcag cagctgacgc gtacacaaca
      301 agtcagcaaa cagacaggtt gaacttcatc cccaaaggag aagctcaact caagcccaag
      361 agctttgcta aggccctaac aagcccacca aagcaaaaag cccactggct cacgctagga
      421 accaaaaggc ccagcagtga tccagcccca aaagagatct cctttgcccc ggagattaca
      481 atggacgatt tcctctatct ttacgatcta ggaaggaagt tcgaaggtga aggtgacgac
      541 actatgttca ccactgataa tgagaaggtt agcctcttca atttcagaaa gaatgctgac
      601 ccacagatgg ttagagaggc ctacgcagca ggtctcatca agacgatcta cccgagtaac
      661 aatctccagg agatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg
      721 actaattgca tcaagaacac agagaaagac atatttctca agatcagaag tactattcca
      781 gtatggacga ttcaaggctt gcttcataaa ccaaggcaag taatagagat tggagtctct
      841 aaaaaggtag ttcctactga atctaaggcc atgcatggag tctaagattc aaatcgagga
      901 tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa
      961 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctggtct actccaaaaa
     1021 tgtcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaaggataat
     1081 ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt
     1141 agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ctatcattca
     1201 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     1261 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaca tctccactga
     1321 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     1381 ttcatttcat ttggagagga cacgctcgag ctcaagcttc gaattctgca gtcgagcacg
     1441 aggtttttct gtctagtgag cagtgtccaa cctcaaaaga caacatgtgt gacgacgatg
     1501 tagcggctct tgtcgtagac aatggatccg gtatgtgcaa agccggtttc gcaggagatg
     1561 acgcaccccg tgccgtcttc ccctcgatcg tcggtcgccc aaggcatcaa ggagtcatgg
     1621 tcggtatggg acaaaaggac tcatacgtag gagatgaagc ccaaagcaaa agaggtatcc
     1681 tcaccctgaa ataccccatc gaacacggta tcatcaccaa ctgggatgac atagatctgg
     1741 tacggaccgt actactctat tcgtttcaat atatttattt gtttcagctg actgcaagat
     1801 tcaaaaattt ctttattatt ttaaattttg tgtcactcaa aaccagataa acaatttgat
     1861 atagaggcac tatatatata catattctcg attatatatg taaatgagtt aacctttttt
     1921 ttccacttaa aattatatag gggatctatg tcatcccagt tggtgatgat accgtgttcg
     1981 atggggtatt tcagggtgag gatacctctt ttgctttggg cttcatctcc tacgtatgag
     2041 tccttttgtc ccataccgac catgactcct tgatgccttg ggcgaccgac gatcgagggg
     2101 aagacggcac ggggtgcgtc atctcctgcg aaaccggctt tgcacatacc ggatccattg
     2161 tctacgacaa gagccgctac atcgtcgtca cacatgttgt cttttgaggt tggacactgc
     2221 tcactagaca gaaaaacctc gtgctctaga gtcctgcttt aatgagatat gcgagacgcc
     2281 tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg
     2341 tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact
     2401 atcgtatttt tatgaataat attctccgtt caatttactg attgtaccct actacttata
     2461 tgtacaatat taaaatgaaa acaatatatt gtgctgaata ggtttatagc gacatctatg
     2521 atagagcgcc acaataacaa acaattgcgt tttattatta caaatccaat tttaaaaaaa
     2581 gcggcagaac cggtcaaacc taaaagactg attacataaa tcttattcaa atttcaaaag
     2641 tgccccaggg gctagtatct acgacacacc gagcggcgaa ctaataacgc tcactgaagg
     2701 gaactccggt tccccgccgg cgcgcatggg tgagattcct tgaagttgag tattggccgt
     2761 ccgctctacc gaaagttacg ggcaccattc aacccggtcc agcacggcgg ccgggtaacc
     2821 gacttgctgc cccgagaatt atgcagcatt tttttggtgt atgtgggccc caaatgaagt
     2881 gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg gtccagggcg aattttgcga
     2941 caacatgtcg aggctcagca ggacctgcag gcatgcaagc tagcttacta gtgatgcata
     3001 ttctatagtg tcacctaaat ctgcggccgc tgaccaagtc agctagcttg gcactggccg
     3061 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     3121 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3181 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg
     3241 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     3301 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt
     3361 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
     3421 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     3481 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     3541 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     3601 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     3661 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     3721 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     3781 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     3841 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     3901 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     3961 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     4021 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     4081 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     4141 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     4201 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     4261 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     4321 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     4381 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     4441 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     4501 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     4561 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     4621 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     4681 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     4741 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     4801 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     4861 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     4921 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     4981 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     5041 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     5101 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     5161 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     5221 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     5281 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     5341 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     5401 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     5461 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     5521 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     5581 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     5641 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     5701 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     5761 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     5821 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     5881 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     5941 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     6001 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     6061 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     6121 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     6181 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     6241 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     6301 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     6361 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     6421 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     6481 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     6541 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     6601 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     6661 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     6721 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     6781 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     6841 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     6901 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     6961 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     7021 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     7081 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     7141 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     7201 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     7261 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     7321 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     7381 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     7441 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     7501 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7561 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7621 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7681 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7741 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7801 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7861 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7921 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7981 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8041 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     8101 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8161 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8221 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8281 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
     8341 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
     8401 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
     8461 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
     8521 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
     8581 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
     8641 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
     8701 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
     8761 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
     8821 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
     8881 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
     8941 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
     9001 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     9061 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     9121 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     9181 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     9241 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     9301 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     9361 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     9421 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     9481 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     9541 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     9601 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     9661 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     9721 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     9781 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     9841 tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc
     9901 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     9961 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
    10021 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
    10081 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc
    10141 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
    10201 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
    10261 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
    10321 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
    10381 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
    10441 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
    10501 tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa
    10561 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
    10621 tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg
    10681 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
    10741 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
    10801 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
    10861 cagacgtcgc ggtgagttca ggctttttca tatctcattg cccccccgga tctgcgaaag
    10921 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
    10981 aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc
    11041 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
    11101 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
    11161 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
    11221 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
    11281 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
    11341 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt
    11401 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
    11461 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
    11521 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
    11581 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
    11641 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
    11701 ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc
    11761 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    11821 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    11881 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    11941 ccatgattac g
//
