LOCUS       V005274                 7650 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005274
VERSION     V005274
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Pest control. Full crop protection from an insect pest by expression
            of long double-stranded RNAs in plastids
  JOURNAL   Science 347 (6225), 991-994 (2015)
   PUBMED   25722411
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) Bock, Max Planck Institute of Molecular
            Plant Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476,
            Germany
REFERENCE   3  (bases 1 to 7650)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7650)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Science";
            date: "2015"; volume: "347"; issue: "6225"; pages: "991-994"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-DEC-2014) Bock, Max Planck Institute of Molecular Plant
            Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     tRNA            31..104
                     /gene="trn-fM"
                     /product="tRNA-Met"
                     /label="trn-fM"
     gene            31..104
                     /gene="trn-fM"
                     /label="trn-fM"
     CDS             complement(257..556)
                     /gene="rps14"
                     /label="Small ribosomal subunit protein uS14c"
                     /note="Small ribosomal subunit protein uS14c from Nicotiana
                     sylvestris. Accession#: Q3C1I0"
     misc_feature    complement(679..1879)
                     /label="3' part of psaB"
                     /note="3' part of psaB"
     promoter        complement(1891..1909)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1919..1935)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2077..2532
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2558..2662
                     /label="AmpR promoter"
     CDS             2663..3520
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3694..4282
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4570..4591
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4606..4636
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4644..4660
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4668..4684
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4705..4723
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    4737..4867
                     /label="3' part of psbZ"
                     /note="3' part of psbZ"
     tRNA            5143..5213
                     /gene="trnG"
                     /product="tRNA-Gly"
                     /label="trnG"
     gene            5143..5213
                     /gene="trnG"
                     /label="trnG"
     primer_bind     5419..5435
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      5444..5560
                     /gene="Prrn"
                     /regulatory_class="promoter"
     gene            5444..5560
                     /gene="Prrn"
                     /label="Prrn"
     misc_feature    5573..5869
                     /note="actin; 'similar to Leptinotarsa decemlineata
                     beta-actin"
     regulatory      complement(5876..5992)
                     /gene="Prrn"
                     /regulatory_class="promoter"
     gene            complement(5876..5992)
                     /gene="Prrn"
                     /label="Prrn"
     primer_bind     complement(6012..6028)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      6053..6328
                     /gene="PpsbA"
                     /label="similar to Chlamydomonas reinhardtii PpsbA"
                     /note="similar to Chlamydomonas reinhardtii PpsbA"
                     /regulatory_class="promoter"
     gene            6053..6328
                     /gene="PpsbA"
                     /label="PpsbA"
     CDS             6335..7123
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     3'UTR           7141..7576
                     /gene="rbcL"
                     /label="similar to Chlamydomonas reinhardtii rbcL 3'UTR"
                     /note="similar to Chlamydomonas reinhardtii rbcL 3'UTR"
     gene            7141..7576
                     /gene="rbcL"
                     /label="rbcL"
     promoter        complement(7597..7615)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aaccctaaaa tagtttggca aaacaagatg ttgcggagac aggatttgaa cccgtgacct
       61 caaggttatg agccttgcga gctaccaaac tgctctaccc cgcgccgaag ataagaactg
      121 aaaactaata gataaacaag gattaaatgc gcccctccac cctatctgta caaatagaat
      181 agcccattta tacagaatgg taaaggggct tctatgatca tcgaccatag aaatagaaat
      241 gaagcgttaa tccttaccaa cttgatcttg ttgctcctgg caacaaacat gcatgaacca
      301 tttcacgaag tatgtgtccg gatagtccaa agtctcgata gttagctctc ggccttccgg
      361 tcaaaaaaca acgtcgatga aggcgtgtag gtgcactatt ccgtggtggg gattgtaact
      421 ttccataaat ttcccatttg tcactcaacg acggaacctt gcttatttct ttctttgagg
      481 atcgacgaat cgaatgatat ttctgttcca atttttgcct cttcttctcc ctctgaatca
      541 aacttttcct tgccataatg gttgaattcc tattagtatc catgatacaa gtcgaatcct
      601 agatgtagaa atagaagaag gtggaccccc tctccgtcga aagaaatgag attatcgcag
      661 atacacacat taaaaatatt aaccaaattt gcccgacgta gaggcaatca agaaagccgc
      721 ataagtgaat atataaccta cagaaaagtg agctaatcca accaatcttg cttgtacaat
      781 ggaaagggcc actggtttat ctctccagcg aatcaaattg gccaaaggtg tgcgttcatg
      841 agcccatgct aaagtttcaa tcaattcctg ccaatatcca cgccaagaaa ttaagaacat
      901 aaatccagta gcccaaacaa gatgtccaaa taagaacatc catgcccaaa ccgataaact
      961 attcatacca aaaggattat atccgttgat aagttgtgaa gagtttaacc ataaataatc
     1021 ccttaaccag cccatcaaat aagtggaaga ttcattaaac tgtgaaacgt taccctgcca
     1081 taatgtgatg tgcttccaat gccaataaaa agtaacccat ccaatagtat ttaacatcca
     1141 aaaaactgcc aaataaaacg cgtcccatgc cgaaatatca caagtaccgc ctcgtcctgg
     1201 gccatcgcac ggaaaactat aaccgaaatc ctttttatct ggcattaact tggaaccacg
     1261 tgcatctaaa gcacctttta ctaagatcaa tgtagttgta tgtaaaccaa gagcaatagc
     1321 atgatgaacc aaaaagtctc caggacctat tgttaaaaat aatgaattac tattttcatt
     1381 aacagcattt aaccaacccg gcaaccagat gcttcgaccc gcattgaatg ctggaccact
     1441 cgttgaagat aaaagtacat cgaacccata tgaagtttta ccatgagcgg attgtatcca
     1501 ttgagcaaat ataggttcaa tcaagatttg cttctccgga gtgccaaagg caagcatgac
     1561 atcattatga acataaagtc ccagggtatg gaatcccaga aagaggctgg cccaacttaa
     1621 atgagatatg atagcttctt tatgctctaa cattcttgcc aatacattat cttcattttg
     1681 ctccggattg taatctctaa tgaaaaatat agctccatga gcaaaagctc ctgtcatgat
     1741 gaatcctgcg atatattggt ggtgggtata taatgcagct tgagtagtaa agtcttgtgc
     1801 tatgaatgca taagcaggta aagagtacat gtgttgagct accaaagaag taataacccc
     1861 taaagaagct agagcaaggc tccaattcgc cctatagtga gtcgtattac gcgcgctcac
     1921 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     1981 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2041 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     2101 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2161 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2221 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2281 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2341 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2401 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2461 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2521 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2581 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2641 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2701 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2761 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     2821 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2881 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2941 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3001 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3061 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3121 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3181 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3241 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3301 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3361 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3421 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3481 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3541 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3601 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3661 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3721 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3781 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3841 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3901 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3961 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4021 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4081 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4141 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4201 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4261 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4321 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4381 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4441 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     4501 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     4561 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     4621 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     4681 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggtgat
     4741 tagcgtaccc gttgtatttg cttctcctga tggctggtca agtaacaaaa atgttgtatt
     4801 ttctggtaca tccttatgga ttggattagt ctttctggtg ggtatcctta attctctcat
     4861 ctcttgaacc tattcgtcgc agacccaaaa ccaaaatgac ccccctaatt tttctcggtt
     4921 gtgagacaca ttaaattgga atctaagtcc ccaaagaaaa cgcaaatcaa ataaagaaaa
     4981 caaaaaaatt agaggggggt caaacttctt gaataaaaag aatacaatta aaaaaataat
     5041 tggaatcgtt ccgaagagaa tatgtgtccc ggcactgcac aaaaaagatc cggttatata
     5101 tcatatatgt gggtacatat tgtgtatcaa gaacaaaaaa atgcggatat ggtcgaatgg
     5161 taaaatttct ctttgccaag gagaagatgc gggttcgatt cccgctatcc gcccaagatc
     5221 caagataaag taattttatt actatttatt tattatttaa tttcataaat agcattaaat
     5281 atatccttaa attaaggatt tggtatagtt ggccgtgata gtgtagtgat tctatccctc
     5341 ccctacgttt tctttttcct tccaccccca aaaagcgaaa ggcgggaatt aattactagt
     5401 tagtaccggg ccccccctcg aggtcgacgg tatcgataag cttgctcccc cgccgtcgtt
     5461 caatgagaat ggataagagg ctcgtgggat tgacgtgagg gggcagggat ggctatattt
     5521 ctgggagcga actccgggcg aatacgaagc gcttggatac gcatgcctgc aggcacgagg
     5581 tttttctgtc tagtgagcag tgtccaacct caaaagacaa catgtgtgac gacgatgtag
     5641 cggctcttgt cgtagacaat ggatccggta tgtgcaaagc cggtttcgca ggagatgacg
     5701 caccccgtgc cgtcttcccc tcgatcgtcg gtcgcccaag gcatcaagga gtcatggtcg
     5761 gtatgggaca aaaggactca tacgtaggag atgaagccca aagcaaaaga ggtatcctca
     5821 ccctgaaata ccccatcgaa cacggtatca tcaccaactg ggatgacatg agctcgtatc
     5881 caagcgcttc gtattcgccc ggagttcgct cccagaaata tagccatccc tgccccctca
     5941 cgtcaatccc acgagcctct tatccattct cattgaacga cggcggggga gcgaattcct
     6001 gcagcccggg ggatccacta gttctagagc ggccgccacc gcggtggagg ggatccaaga
     6061 aaagtgagct attaacgcgt cctattttaa tactccgaag gaggcagttg gcaggcaact
     6121 gccactgacg tcccgtaagg gtaaggggac gtccactggc gtcccgtaag gggaagggga
     6181 cgtaggtaca taaatgtgct aggtaactaa cgtttgattt tttgtggtat aatatatgta
     6241 ccatgctttt aatagaagct tgaatttata aattaaaata tttttacaat attttacgga
     6301 gaaattaaaa ctttaaaaaa attaacatat gaccatgggg gaagcggtga tcgccgaagt
     6361 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     6421 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     6481 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     6541 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     6601 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     6661 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     6721 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     6781 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     6841 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     6901 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     6961 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     7021 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     7081 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagggcatgc
     7141 aagcttgtac tcaagctcgt aacgaaggtc gtgaccttgc tcgtgaaggt ggcgacgtaa
     7201 ttcgttcagc ttgtaaatgg tctccagaac ttgctgctgc atgtgaagtt tggaaagaaa
     7261 ttaaattcga atttgatact attgacaaac tttaattttt atttttcatg atgtttatgt
     7321 gaatagcata aacatcgttt ttatttttat ggtgtttagg ttaaatacct aaacatcatt
     7381 ttacattttt aaaattaagt tctaaagtta tcttttgttt aaatttgcct gtctttataa
     7441 attacgatgt gccagaaaaa taaaatctta gctttttatt atagaattta tctttatgta
     7501 ttatatttta taagttataa taaaagaaat agtaacatac taaagcggat gtaactcaat
     7561 cggtagagtg cgatccacta gctccagctt ttgttccctt tagtgagggt taattgcgcg
     7621 cttggcgtaa tcatggtcat agctgtttcc
//
