LOCUS       V005275                 8709 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005275
VERSION     V005275
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Pest control. Full crop protection from an insect pest by expression
            of long double-stranded RNAs in plastids
  JOURNAL   Science 347 (6225), 991-994 (2015)
   PUBMED   25722411
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) Bock, Max Planck Institute of Molecular
            Plant Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476,
            Germany
REFERENCE   3  (bases 1 to 8709)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Science";
            date: "2015"; volume: "347"; issue: "6225"; pages: "991-994"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-DEC-2014) Bock, Max Planck Institute of Molecular Plant
            Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     tRNA            31..104
                     /gene="trn-fM"
                     /product="tRNA-Met"
                     /label="trn-fM"
     gene            31..104
                     /gene="trn-fM"
                     /label="trn-fM"
     CDS             complement(257..556)
                     /gene="rps14"
                     /label="Small ribosomal subunit protein uS14c"
                     /note="Small ribosomal subunit protein uS14c from Nicotiana
                     sylvestris. Accession#: Q3C1I0"
     misc_feature    complement(679..1879)
                     /label="3' part of psaB"
                     /note="3' part of psaB"
     promoter        complement(1891..1909)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1919..1935)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2077..2532
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2558..2662
                     /label="AmpR promoter"
     CDS             2663..3520
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3694..4282
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4570..4591
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4606..4636
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4644..4660
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4668..4684
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4705..4723
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    4737..4867
                     /label="3' part of psbZ"
                     /note="3' part of psbZ"
     tRNA            5143..5213
                     /gene="trnG"
                     /product="tRNA-Gly"
                     /label="trnG"
     gene            5143..5213
                     /gene="trnG"
                     /label="trnG"
     primer_bind     5418..5434
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      5443..5559
                     /gene="Prrn"
                     /regulatory_class="promoter"
     gene            5443..5559
                     /gene="Prrn"
                     /label="Prrn"
     misc_feature    5572..5868
                     /note="actin; similar to Leptinotarsa decemlineata
                     beta-actin"
     misc_feature    5869..6088
                     /note="shrub; similar to Leptinotarsa decemlineata shrub
                     gene"
     intron          6096..6296
                     /gene="St-GA20 ox"
                     /number=1
                     /note="first intron from potato GA20 oxidase gene"
     gene            6096..6296
                     /gene="St-GA20 ox"
                     /label="St-GA20 ox"
                     /note="GA20 oxidase"
     misc_feature    complement(6310..6529)
                     /label="similar to Leptinotarsa decemlineata shrub gene"
                     /note="similar to Leptinotarsa decemlineata shrub gene"
     misc_feature    complement(6530..6826)
                     /label="similar to Leptinotarsa decemlineata actin gene"
                     /note="similar to Leptinotarsa decemlineata actin gene"
     terminator      6835..6921
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      7013..7040
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     primer_bind     complement(7071..7087)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      7112..7387
                     /gene="PpsbA"
                     /label="similar to Chlamydomonas reinhardtii psbA gene"
                     /note="similar to Chlamydomonas reinhardtii psbA gene"
                     /regulatory_class="promoter"
     gene            7112..7387
                     /gene="PpsbA"
                     /label="PpsbA"
     CDS             7394..8182
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     3'UTR           8200..8635
                     /gene="rbcL"
                     /label="similar to Chlamydomonas reinhardtii rbcL gene"
                     /note="similar to Chlamydomonas reinhardtii rbcL gene"
     gene            8200..8635
                     /gene="rbcL"
                     /label="rbcL"
     promoter        complement(8656..8674)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aaccctaaaa tagtttggca aaacaagatg ttgcggagac aggatttgaa cccgtgacct
       61 caaggttatg agccttgcga gctaccaaac tgctctaccc cgcgccgaag ataagaactg
      121 aaaactaata gataaacaag gattaaatgc gcccctccac cctatctgta caaatagaat
      181 agcccattta tacagaatgg taaaggggct tctatgatca tcgaccatag aaatagaaat
      241 gaagcgttaa tccttaccaa cttgatcttg ttgctcctgg caacaaacat gcatgaacca
      301 tttcacgaag tatgtgtccg gatagtccaa agtctcgata gttagctctc ggccttccgg
      361 tcaaaaaaca acgtcgatga aggcgtgtag gtgcactatt ccgtggtggg gattgtaact
      421 ttccataaat ttcccatttg tcactcaacg acggaacctt gcttatttct ttctttgagg
      481 atcgacgaat cgaatgatat ttctgttcca atttttgcct cttcttctcc ctctgaatca
      541 aacttttcct tgccataatg gttgaattcc tattagtatc catgatacaa gtcgaatcct
      601 agatgtagaa atagaagaag gtggaccccc tctccgtcga aagaaatgag attatcgcag
      661 atacacacat taaaaatatt aaccaaattt gcccgacgta gaggcaatca agaaagccgc
      721 ataagtgaat atataaccta cagaaaagtg agctaatcca accaatcttg cttgtacaat
      781 ggaaagggcc actggtttat ctctccagcg aatcaaattg gccaaaggtg tgcgttcatg
      841 agcccatgct aaagtttcaa tcaattcctg ccaatatcca cgccaagaaa ttaagaacat
      901 aaatccagta gcccaaacaa gatgtccaaa taagaacatc catgcccaaa ccgataaact
      961 attcatacca aaaggattat atccgttgat aagttgtgaa gagtttaacc ataaataatc
     1021 ccttaaccag cccatcaaat aagtggaaga ttcattaaac tgtgaaacgt taccctgcca
     1081 taatgtgatg tgcttccaat gccaataaaa agtaacccat ccaatagtat ttaacatcca
     1141 aaaaactgcc aaataaaacg cgtcccatgc cgaaatatca caagtaccgc ctcgtcctgg
     1201 gccatcgcac ggaaaactat aaccgaaatc ctttttatct ggcattaact tggaaccacg
     1261 tgcatctaaa gcacctttta ctaagatcaa tgtagttgta tgtaaaccaa gagcaatagc
     1321 atgatgaacc aaaaagtctc caggacctat tgttaaaaat aatgaattac tattttcatt
     1381 aacagcattt aaccaacccg gcaaccagat gcttcgaccc gcattgaatg ctggaccact
     1441 cgttgaagat aaaagtacat cgaacccata tgaagtttta ccatgagcgg attgtatcca
     1501 ttgagcaaat ataggttcaa tcaagatttg cttctccgga gtgccaaagg caagcatgac
     1561 atcattatga acataaagtc ccagggtatg gaatcccaga aagaggctgg cccaacttaa
     1621 atgagatatg atagcttctt tatgctctaa cattcttgcc aatacattat cttcattttg
     1681 ctccggattg taatctctaa tgaaaaatat agctccatga gcaaaagctc ctgtcatgat
     1741 gaatcctgcg atatattggt ggtgggtata taatgcagct tgagtagtaa agtcttgtgc
     1801 tatgaatgca taagcaggta aagagtacat gtgttgagct accaaagaag taataacccc
     1861 taaagaagct agagcaaggc tccaattcgc cctatagtga gtcgtattac gcgcgctcac
     1921 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     1981 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2041 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     2101 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2161 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2221 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2281 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2341 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2401 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2461 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2521 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2581 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2641 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2701 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2761 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     2821 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2881 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2941 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3001 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3061 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3121 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3181 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3241 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3301 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3361 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3421 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3481 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3541 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3601 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3661 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3721 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3781 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3841 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3901 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3961 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4021 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4081 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4141 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4201 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4261 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4321 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4381 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4441 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     4501 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     4561 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     4621 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     4681 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggtgat
     4741 tagcgtaccc gttgtatttg cttctcctga tggctggtca agtaacaaaa atgttgtatt
     4801 ttctggtaca tccttatgga ttggattagt ctttctggtg ggtatcctta attctctcat
     4861 ctcttgaacc tattcgtcgc agacccaaaa ccaaaatgac ccccctaatt tttctcggtt
     4921 gtgagacaca ttaaattgga atctaagtcc ccaaagaaaa cgcaaatcaa ataaagaaaa
     4981 caaaaaaatt agaggggggt caaacttctt gaataaaaag aatacaatta aaaaaataat
     5041 tggaatcgtt ccgaagagaa tatgtgtccc ggcactgcac aaaaaagatc cggttatata
     5101 tcatatatgt gggtacatat tgtgtatcaa gaacaaaaaa atgcggatat ggtcgaatgg
     5161 taaaatttct ctttgccaag gagaagatgc gggttcgatt cccgctatcc gcccaagatc
     5221 caagataaag taattttatt actatttatt tattatttaa tttcataaat agcattaaat
     5281 atatccttaa attaaggatt tggtatagtt ggccgtgata gtgtagtgat tctatccctc
     5341 ccctacgttt tctttttcct tccaccccca aaaagcgaaa ggcgggaatt aattactagt
     5401 tgtaccgggc cccccctcga ggtcgacggt atcgataagc ttgctccccc gccgtcgttc
     5461 aatgagaatg gataagaggc tcgtgggatt gacgtgaggg ggcagggatg gctatatttc
     5521 tgggagcgaa ctccgggcga atacgaagcg cttggatacg catgcctgca ggcacgaggt
     5581 ttttctgtct agtgagcagt gtccaacctc aaaagacaac atgtgtgacg acgatgtagc
     5641 ggctcttgtc gtagacaatg gatccggtat gtgcaaagcc ggtttcgcag gagatgacgc
     5701 accccgtgcc gtcttcccct cgatcgtcgg tcgcccaagg catcaaggag tcatggtcgg
     5761 tatgggacaa aaggactcat acgtaggaga tgaagcccaa agcaaaagag gtatcctcac
     5821 cctgaaatac cccatcgaac acggtatcat caccaactgg gatgacatgc aatgtcatcc
     5881 atcatgtcgt gtacattgtc cacgtccaag tttttatggg ctttcttaag agcttcagct
     5941 gcatttttca tagattccaa tactgtggtg ttcgtactag ctccctccag agcttctcgt
     6001 tgaagttcaa tagtagttaa agtgccatct atttgcaact gatttttttc taatcgcttc
     6061 ttccgcttca gcgcttgcat ggccgctcag atctggtacg gaccgtacta ctctattcgt
     6121 ttcaatatat ttatttgttt cagctgactg caagattcaa aaatttcttt attattttaa
     6181 attttgtgtc actcaaaacc agataaacaa tttgatatag aggcactata tatatacata
     6241 ttctcgatta tatatgtaaa tgagttaacc ttttttttcc acttaaaatt atataggggg
     6301 atccccgggg agcggccatg caagcgctga agcggaagaa gcgattagaa aaaaatcagt
     6361 tgcaaataga tggcacttta actactattg aacttcaacg agaagctctg gagggagcta
     6421 gtacgaacac cacagtattg gaatctatga aaaatgcagc tgaagctctt aagaaagccc
     6481 ataaaaactt ggacgtggac aatgtacacg acatgatgga tgacattgca tgtcatccca
     6541 gttggtgatg ataccgtgtt cgatggggta tttcagggtg aggatacctc ttttgctttg
     6601 ggcttcatct cctacgtatg agtccttttg tcccataccg accatgactc cttgatgcct
     6661 tgggcgaccg acgatcgagg ggaagacggc acggggtgcg tcatctcctg cgaaaccggc
     6721 tttgcacata ccggatccat tgtctacgac aagagccgct acatcgtcgt cacacatgtt
     6781 gtcttttgag gttggacact gctcactaga cagaaaaacc tcgtgcgagc tcatcaaata
     6841 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     6901 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     6961 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc
     7021 atcctgacgg atggcctttt tgcgtttcta cgaattcctg cagcccgggg gatccactag
     7081 ttctagagcg gccgccaccg cggtggaggg gatccaagaa aagtgagcta ttaacgcgtc
     7141 ctattttaat actccgaagg aggcagttgg caggcaactg ccactgacgt cccgtaaggg
     7201 taaggggacg tccactggcg tcccgtaagg ggaaggggac gtaggtacat aaatgtgcta
     7261 ggtaactaac gtttgatttt ttgtggtata atatatgtac catgctttta atagaagctt
     7321 gaatttataa attaaaatat ttttacaata ttttacggag aaattaaaac tttaaaaaaa
     7381 ttaacatatg accatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     7441 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     7501 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     7561 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     7621 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     7681 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     7741 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     7801 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     7861 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     7921 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     7981 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     8041 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     8101 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     8161 gatcaccaag gtagtcggca aataatgtct agggcatgca agcttgtact caagctcgta
     8221 acgaaggtcg tgaccttgct cgtgaaggtg gcgacgtaat tcgttcagct tgtaaatggt
     8281 ctccagaact tgctgctgca tgtgaagttt ggaaagaaat taaattcgaa tttgatacta
     8341 ttgacaaact ttaattttta tttttcatga tgtttatgtg aatagcataa acatcgtttt
     8401 tatttttatg gtgtttaggt taaataccta aacatcattt tacattttta aaattaagtt
     8461 ctaaagttat cttttgttta aatttgcctg tctttataaa ttacgatgtg ccagaaaaat
     8521 aaaatcttag ctttttatta tagaatttat ctttatgtat tatattttat aagttataat
     8581 aaaagaaata gtaacatact aaagcggatg taactcaatc ggtagagtgc gatccactag
     8641 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     8701 gctgtttcc
//
