LOCUS       V005277                 7918 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005277
VERSION     V005277
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Pest control. Full crop protection from an insect pest by expression
            of long double-stranded RNAs in plastids
  JOURNAL   Science 347 (6225), 991-994 (2015)
   PUBMED   25722411
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) Bock, Max Planck Institute of Molecular
            Plant Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476,
            Germany
REFERENCE   3  (bases 1 to 7918)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7918)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Science";
            date: "2015"; volume: "347"; issue: "6225"; pages: "991-994"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-DEC-2014) Bock, Max Planck Institute of Molecular Plant
            Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     tRNA            31..104
                     /gene="trn-fM"
                     /product="tRNA-Met"
                     /label="trn-fM"
     gene            31..104
                     /gene="trn-fM"
                     /label="trn-fM"
     CDS             complement(257..556)
                     /gene="rps14"
                     /label="Small ribosomal subunit protein uS14c"
                     /note="Small ribosomal subunit protein uS14c from Nicotiana
                     sylvestris. Accession#: Q3C1I0"
     misc_feature    complement(679..1879)
                     /label="3' part of psaB"
                     /note="3' part of psaB"
     promoter        complement(1891..1909)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1919..1935)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2077..2532
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2558..2662
                     /label="AmpR promoter"
     CDS             2663..3520
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3694..4282
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4570..4591
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4606..4636
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4644..4660
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4668..4684
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4705..4723
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    4737..4867
                     /label="3' part of psbZ"
                     /note="3' part of psbZ"
     tRNA            5143..5213
                     /gene="trnG"
                     /product="tRNA-Gly"
                     /label="trnG"
     gene            5143..5213
                     /gene="trnG"
                     /label="trnG"
     primer_bind     5419..5435
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      5444..5560
                     /gene="Prrn"
                     /regulatory_class="promoter"
     gene            5444..5560
                     /gene="Prrn"
                     /label="Prrn"
     misc_feature    5597..5893
                     /note="actin; similar to Leptinotarsa decemlineata
                     beta-actin"
     misc_feature    5894..6113
                     /note="shrub; similar to Leptinotarsa decemlineata shrub
                     gene"
     regulatory      complement(6144..6260)
                     /gene="Prrn"
                     /regulatory_class="promoter"
     gene            complement(6144..6260)
                     /gene="Prrn"
                     /label="Prrn"
     primer_bind     complement(6280..6296)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      6321..6596
                     /gene="PpsbA"
                     /label="similar to Chlamydomonas reinhardtii PpsbA"
                     /note="similar to Chlamydomonas reinhardtii PpsbA"
                     /regulatory_class="promoter"
     gene            6321..6596
                     /gene="PpsbA"
                     /label="PpsbA"
     CDS             6603..7391
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     3'UTR           7409..7844
                     /gene="rbcL"
                     /label="similar to Chlamydomonas reinhardtii rbcL 3'UTR"
                     /note="similar to Chlamydomonas reinhardtii rbcL 3'UTR"
     gene            7409..7844
                     /gene="rbcL"
                     /label="rbcL"
     promoter        complement(7865..7883)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aaccctaaaa tagtttggca aaacaagatg ttgcggagac aggatttgaa cccgtgacct
       61 caaggttatg agccttgcga gctaccaaac tgctctaccc cgcgccgaag ataagaactg
      121 aaaactaata gataaacaag gattaaatgc gcccctccac cctatctgta caaatagaat
      181 agcccattta tacagaatgg taaaggggct tctatgatca tcgaccatag aaatagaaat
      241 gaagcgttaa tccttaccaa cttgatcttg ttgctcctgg caacaaacat gcatgaacca
      301 tttcacgaag tatgtgtccg gatagtccaa agtctcgata gttagctctc ggccttccgg
      361 tcaaaaaaca acgtcgatga aggcgtgtag gtgcactatt ccgtggtggg gattgtaact
      421 ttccataaat ttcccatttg tcactcaacg acggaacctt gcttatttct ttctttgagg
      481 atcgacgaat cgaatgatat ttctgttcca atttttgcct cttcttctcc ctctgaatca
      541 aacttttcct tgccataatg gttgaattcc tattagtatc catgatacaa gtcgaatcct
      601 agatgtagaa atagaagaag gtggaccccc tctccgtcga aagaaatgag attatcgcag
      661 atacacacat taaaaatatt aaccaaattt gcccgacgta gaggcaatca agaaagccgc
      721 ataagtgaat atataaccta cagaaaagtg agctaatcca accaatcttg cttgtacaat
      781 ggaaagggcc actggtttat ctctccagcg aatcaaattg gccaaaggtg tgcgttcatg
      841 agcccatgct aaagtttcaa tcaattcctg ccaatatcca cgccaagaaa ttaagaacat
      901 aaatccagta gcccaaacaa gatgtccaaa taagaacatc catgcccaaa ccgataaact
      961 attcatacca aaaggattat atccgttgat aagttgtgaa gagtttaacc ataaataatc
     1021 ccttaaccag cccatcaaat aagtggaaga ttcattaaac tgtgaaacgt taccctgcca
     1081 taatgtgatg tgcttccaat gccaataaaa agtaacccat ccaatagtat ttaacatcca
     1141 aaaaactgcc aaataaaacg cgtcccatgc cgaaatatca caagtaccgc ctcgtcctgg
     1201 gccatcgcac ggaaaactat aaccgaaatc ctttttatct ggcattaact tggaaccacg
     1261 tgcatctaaa gcacctttta ctaagatcaa tgtagttgta tgtaaaccaa gagcaatagc
     1321 atgatgaacc aaaaagtctc caggacctat tgttaaaaat aatgaattac tattttcatt
     1381 aacagcattt aaccaacccg gcaaccagat gcttcgaccc gcattgaatg ctggaccact
     1441 cgttgaagat aaaagtacat cgaacccata tgaagtttta ccatgagcgg attgtatcca
     1501 ttgagcaaat ataggttcaa tcaagatttg cttctccgga gtgccaaagg caagcatgac
     1561 atcattatga acataaagtc ccagggtatg gaatcccaga aagaggctgg cccaacttaa
     1621 atgagatatg atagcttctt tatgctctaa cattcttgcc aatacattat cttcattttg
     1681 ctccggattg taatctctaa tgaaaaatat agctccatga gcaaaagctc ctgtcatgat
     1741 gaatcctgcg atatattggt ggtgggtata taatgcagct tgagtagtaa agtcttgtgc
     1801 tatgaatgca taagcaggta aagagtacat gtgttgagct accaaagaag taataacccc
     1861 taaagaagct agagcaaggc tccaattcgc cctatagtga gtcgtattac gcgcgctcac
     1921 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     1981 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2041 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     2101 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2161 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2221 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2281 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2341 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2401 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2461 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2521 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2581 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2641 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2701 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2761 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     2821 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2881 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2941 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3001 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3061 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3121 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3181 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3241 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3301 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3361 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3421 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3481 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3541 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3601 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3661 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3721 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3781 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3841 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3901 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3961 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4021 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4081 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4141 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4201 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4261 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4321 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4381 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4441 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     4501 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     4561 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     4621 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     4681 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggtgat
     4741 tagcgtaccc gttgtatttg cttctcctga tggctggtca agtaacaaaa atgttgtatt
     4801 ttctggtaca tccttatgga ttggattagt ctttctggtg ggtatcctta attctctcat
     4861 ctcttgaacc tattcgtcgc agacccaaaa ccaaaatgac ccccctaatt tttctcggtt
     4921 gtgagacaca ttaaattgga atctaagtcc ccaaagaaaa cgcaaatcaa ataaagaaaa
     4981 caaaaaaatt agaggggggt caaacttctt gaataaaaag aatacaatta aaaaaataat
     5041 tggaatcgtt ccgaagagaa tatgtgtccc ggcactgcac aaaaaagatc cggttatata
     5101 tcatatatgt gggtacatat tgtgtatcaa gaacaaaaaa atgcggatat ggtcgaatgg
     5161 taaaatttct ctttgccaag gagaagatgc gggttcgatt cccgctatcc gcccaagatc
     5221 caagataaag taattttatt actatttatt tattatttaa tttcataaat agcattaaat
     5281 atatccttaa attaaggatt tggtatagtt ggccgtgata gtgtagtgat tctatccctc
     5341 ccctacgttt tctttttcct tccaccccca aaaagcgaaa ggcgggaatt aattactagt
     5401 tagtaccggg ccccccctcg aggtcgacgg tatcgataag cttgctcccc cgccgtcgtt
     5461 caatgagaat ggataagagg ctcgtgggat tgacgtgagg gggcagggat ggctatattt
     5521 ctgggagcga actccgggcg aatacgaagc gcttggatac gcatgcgggt gggtggaaaa
     5581 ccacccaccc ctgcaggcac gaggtttttc tgtctagtga gcagtgtcca acctcaaaag
     5641 acaacatgtg tgacgacgat gtagcggctc ttgtcgtaga caatggatcc ggtatgtgca
     5701 aagccggttt cgcaggagat gacgcacccc gtgccgtctt cccctcgatc gtcggtcgcc
     5761 caaggcatca aggagtcatg gtcggtatgg gacaaaagga ctcatacgta ggagatgaag
     5821 cccaaagcaa aagaggtatc ctcaccctga aataccccat cgaacacggt atcatcacca
     5881 actgggatga catgcaatgt catccatcat gtcgtgtaca ttgtccacgt ccaagttttt
     5941 atgggctttc ttaagagctt cagctgcatt tttcatagat tccaatactg tggtgttcgt
     6001 actagctccc tccagagctt ctcgttgaag ttcaatagta gttaaagtgc catctatttg
     6061 caactgattt ttttctaatc gcttcttccg cttcagcgct tgcatggccg ctcagatccc
     6121 gcacgctcct aatggagcgt gcggtatcca agcgcttcgt attcgcccgg agttcgctcc
     6181 cagaaatata gccatccctg ccccctcacg tcaatcccac gagcctctta tccattctca
     6241 ttgaacgacg gcgggggagc gaattcctgc agcccggggg atccactagt tctagagcgg
     6301 ccgccaccgc ggtggagggg atccaagaaa agtgagctat taacgcgtcc tattttaata
     6361 ctccgaagga ggcagttggc aggcaactgc cactgacgtc ccgtaagggt aaggggacgt
     6421 ccactggcgt cccgtaaggg gaaggggacg taggtacata aatgtgctag gtaactaacg
     6481 tttgattttt tgtggtataa tatatgtacc atgcttttaa tagaagcttg aatttataaa
     6541 ttaaaatatt tttacaatat tttacggaga aattaaaact ttaaaaaaat taacatatga
     6601 ccatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     6661 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     6721 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     6781 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     6841 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     6901 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     6961 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     7021 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     7081 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     7141 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     7201 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     7261 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     7321 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     7381 tagtcggcaa ataatgtcta gggcatgcaa gcttgtactc aagctcgtaa cgaaggtcgt
     7441 gaccttgctc gtgaaggtgg cgacgtaatt cgttcagctt gtaaatggtc tccagaactt
     7501 gctgctgcat gtgaagtttg gaaagaaatt aaattcgaat ttgatactat tgacaaactt
     7561 taatttttat ttttcatgat gtttatgtga atagcataaa catcgttttt atttttatgg
     7621 tgtttaggtt aaatacctaa acatcatttt acatttttaa aattaagttc taaagttatc
     7681 ttttgtttaa atttgcctgt ctttataaat tacgatgtgc cagaaaaata aaatcttagc
     7741 tttttattat agaatttatc tttatgtatt atattttata agttataata aaagaaatag
     7801 taacatacta aagcggatgt aactcaatcg gtagagtgcg atccactagc tccagctttt
     7861 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcc
//
