LOCUS       40924_26396        7438 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJY-mini-Tn7T-gus-Sm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Yao J, Allen C.
  TITLE     VsrAD is a major regulator of diverse virulence and fitness 
            functions in the plant pathogen Ralstonia solanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Yao J, Allen C.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2007) Department of Plant Pathology, University of
            Wisconsin-Madison, 1630 Linded Drive, Madison, WI 53706, USA
REFERENCE   3  (bases 1 to 7438)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7438)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2007) Department of Plant Pathology, University of 
            Wisconsin-Madison, 1630 Linded Drive, Madison, WI 53706, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   382..580
                     /note="Tn7R; right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    946..1031
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      1036..1041
                     /regulatory_class="ribosome_binding_site"
     CDS             1050..2858
                     /label=GUS
                     /note="beta-glucuronidase"
     CDS             3761..4549
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     mobile_element  complement(4955..5120)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(5279..5387)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(5619..6207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6381..7238)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7239..7343)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcag gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atatcatgca tgagctcact
      961 agtggatccc ccgggctgca ggaattcctc gagaagcttg ggcccggtac ctcgcgaagg
     1021 gatccctcga gctctagagg atctcgacca tggtccgtcc tgtagaaacc ccaacccgtg
     1081 aaatcaaaaa actcgacggc ctgtgggcat tcagtctgga tcgcgaaaac tgtggaattg
     1141 atcagcgttg gtgggaaagc gcgttacaag aaagccgggc aattgctgtg ccaggcagtt
     1201 ttaacgatca gttcgccgat gcagatattc gtaattatgc gggcaacgtc tggtatcagc
     1261 gcgaagtctt tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg
     1321 tcactcatta cggcaaagtg tgggtcaata atcaggaagt gatggagcat cagggcggct
     1381 atacgccatt tgaagccgat gtcacgccgt atgttattgc cgggaaaagt gtacgtatca
     1441 ccgtttgtgt gaacaacgaa ctgaactggc agactatccc gccgggaatg gtgattaccg
     1501 acgaaaacgg caagaaaaag cagtcttact tccatgattt ctttaactat gccggaatcc
     1561 atcgcagcgt aatgctctac accacgccga acacctgggt ggacgatatc accgtggtga
     1621 cgcatgtcgc gcaagactgt aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg
     1681 atgtcagcgt tgaactgcgt gatgcggatc aacaggtggt tgcaactgga caaggcacta
     1741 gcgggacttt gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt tatctctatg
     1801 aactgtgcgt cacagccaaa agccagacag agtgtgatat ctacccgctt cgcgtcggca
     1861 tccggtcagt ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg ttctacttta
     1921 ctggctttgg tcgtcatgaa gatgcggact tacgtggcaa aggattcgat aacgtgctga
     1981 tggtgcacga ccacgcatta atggactgga ttggggccaa ctcctaccgt acctcgcatt
     2041 acccttacgc tgaagagatg ctcgactggg cagatgaaca tggcatcgtg gtgattgatg
     2101 aaactgctgc tgtcggcttt aacctctctt taggcattgg tttcgaagcg ggcaacaagc
     2161 cgaaagaact gtacagcgaa gaggcagtca acggggaaac tcagcaagcg cacttacagg
     2221 cgattaaaga gctgatagcg cgtgacaaaa accacccaag cgtggtgatg tggagtattg
     2281 ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag
     2341 caacgcgtaa actcgacccg acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg
     2401 ctcacaccga taccatcagc gatctctttg atgtgctgtg cctgaaccgt tattacggat
     2461 ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt actggaaaaa gaacttctgg
     2521 cctggcagga gaaactgcat cagccgatta tcatcaccga atacggcgtg gatacgttag
     2581 ccgggctgca ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt gcatggctgg
     2641 atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt
     2701 tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag aaagggatct
     2761 tcactcgcga ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc tggactggca
     2821 tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg aatcaacaac tctcctggcg
     2881 caccatcgtc ggctacagcc tcgggaatta gctttatgct tgtaaaccgt tttgtgaaaa
     2941 aatttttaaa ataaaaaagg ggacctctag ggtccccaat taattagtaa tataatctat
     3001 taaaggtcat tcaaaaggtc atccaccgga tcaattcccc tgctcgcgca ggctgggtgc
     3061 caagctctcg ggtaacatca aggcccgatc cttggagccc ttgccctccc gcacgatgat
     3121 cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc aaaccctcac tgatccgcat
     3181 gcccgttcca tacagaagct gggcgaacaa acgatgctcg ccttccagaa aaccgaggat
     3241 gcgaaccact tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc gaggtcttcc
     3301 gatctcctga agccagggca gatccgtgca cagcaccttg ccgtagaaga acagcaaggc
     3361 cgccaatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag
     3421 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
     3481 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     3541 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     3601 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     3661 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     3721 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     3781 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     3841 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     3901 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     3961 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     4021 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     4081 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     4141 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     4201 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     4261 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     4321 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     4381 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     4441 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     4501 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac
     4561 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     4621 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     4681 gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta
     4741 tcgcaatagt tggcgaagta atcgcaacat ccgcattaaa atctagcgag ggctttacta
     4801 agctgatccg gtggatgacc ttttgaatga cctttaatag attatattac taattaattg
     4861 gggaccctag aggtcccctt ttttatttta aaaatttttt cacaaaacgg tttacaagca
     4921 taaagctaat tcgatatcaa gctccttgca ggccaaccag ataagtgaaa tctagttcca
     4981 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
     5041 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
     5101 aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgtttgg gcgctgcatt
     5161 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5221 cgctcactga cccgctgcgc tcggtcgttc ggctgcggcg agcggtatca gagcttatcg
     5281 gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga
     5341 agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt
     5401 ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa
     5461 aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa
     5521 gtatacctta aggaatcccc tgataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5641 gacgagcatc acaaaaatcg acgctcgagt cagaggtggc gaaacccgac aggactataa
     5701 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5761 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5821 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5881 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5941 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6001 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     6061 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6121 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6181 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6241 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6301 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6361 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6421 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6481 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6541 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6601 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6661 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6721 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6781 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6841 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6901 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6961 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7021 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7081 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7141 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7201 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7261 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7321 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     7381 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
