LOCUS       40924_26391        3839 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJW3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Javid B, Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2012) Centre for Infectious Diseases Research, 
            Tsinghua University School of Medicine, Lab 4303, Biotechnology 
            Building, Tsinghua University, Haidian District, Beijing, Beijing 
            100084, China
REFERENCE   2  (bases 1 to 3839)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3839)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-AUG-2012) Centre for Infectious Diseases Research, Tsinghua 
            University School of Medicine, Lab 4303, Biotechnology Building, 
            Tsinghua University, Haidian District, Beijing, Beijing 100084, 
            China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..1902
                     /label=oriM
                     /note="oriM"
     regulatory      1909..2053
                     /label=TB HSP70 promoter
                     /note="TB HSP70 promoter"
                     /regulatory_class="promoter"
     CDS             2054..2425
                     /label=BleoR
                     /note="antibiotic-binding protein"
     CDS             2426..2449
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     rep_origin      complement(2507..2863)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     primer_bind     complement(2897..2913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3026..3832
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gtcgacgtga gcccaccagc tccgtaagtt cgggtgctgt gtggctcgta cccgcgcatt
       61 caggcggcag ggggtctaac gggtctaagg cggcgtgtac ggccgccaca gcggctctta
      121 gcggcccgga aacgtcctcg aaacgacgca tgtgttcctc ctggttggta caggtggttg
      181 ggggtgctcg gctgtcgctg gtgtttcatc atcagggctc gacgggagag cgggggagtg
      241 tgcagttgtg gggtggcccc tcagcgaaat atctgacttg gagctcgtgt cggaccatac
      301 accggtgatt aatcgtggtt tattatcaag cgtgagccac gtcgccgacg aatttgagca
      361 gctctggctg ccgtactggt ccctggcaag cgacgatctg ctcgagggga tctaccgcca
      421 aagccgcgcg tcggccctag gccgccggta catcgaggcg aacccaacag cgctggcaaa
      481 cctgctggtc gtggacgtag accatccaga cgcagcgctc cgagcgctca gcgcccgggg
      541 gtcccatccg ctgcccaacg cgatcgtggg caatcgcgcc aacggccacg cacacgcagt
      601 gtgggcactc aacgcccctg ttccacgcac cgaatacgcg cggcgtaagc cgctcgcata
      661 catggcggcg tgcgccgaag gccttcggcg cgccgtcgat ggcgaccgca gttactcagg
      721 cctcatgacc aaaaaccccg gccacatcgc ctgggaaacg gaatggctcc actcagatct
      781 ctacacactc agccacatcg aggccgagct cggcgcgaac atgccaccgc cgcgctggcg
      841 tcagcagacc acgtacaaag cggctccgac gccgctaggg cggaattgcg cactgttcga
      901 ttccgtcagg ttgtgggcct atcttcccgc cctcatgcgg atctacctgc cgacccggaa
      961 cgtggacgga ctcggccgcg cgatctatgc cgagtgccac gcgcgaaacg ccgaatttcc
     1021 gtgcaacgac gtgtgtcccg gaccgctacc ggacagcgag gtccgcgcca tcgccaacag
     1081 catttggcgt tggatcacaa ccaagtcgcg catttgggcg gacgggatcg tggtctacga
     1141 ggccacactc agtgcgcgcc atgcggccat ctcgcggaag ggcgcagcag cgcgcacggc
     1201 ggcgagcaca gttgcgcggc gcgcaaagtc cgcgtcagcc atggaggcat tgctatgagc
     1261 gacggctaca gcgacggcta cagcgacggc tacaactggc agccgactgt ccgcaaaaag
     1321 cggcgcgtga ccgccgccga aggcgctcga atcaccggac tatccgaacg ccacgtcgtc
     1381 cggctcgtgg cgcaggaacg cagcgagtgg ttcgccgagc aggctgcacg ccgcgaacgc
     1441 atccgcgcct atcacgacga cgagggccac tcttggccgc aaacggccaa acatttcggg
     1501 ctgcatctgg acaccgttaa gcgactcggc tatcgggcga ggaaagagcg tgcggcagaa
     1561 caggaagcgg ctcaaaaggc ccacaacgaa gccgacaatc caccgctgtt ctaacgcaat
     1621 tggggagcgg gtgtcgcggg ggttccgtgg ggggttccgt tgcaacgggt cggacaggta
     1681 aaagtcctgg tagacgctag ttttctggtt tgggccatgc ctgtctcgtt gcgtgtttcg
     1741 ttgcgtccgt tttgaatacc agccagacga gacggggttc tacgaatctt ggtcgatacc
     1801 aagccatttc cgctgaatat cgtggagctc accgccagaa tcggtggttg tggtgatgta
     1861 cgtggcgaac tccgttgtag tgcttgtggt ggcatccgtg gctctagagc acgaccagcg
     1921 ttagcatgct cagtaagttg agtgcatcag gctcagctct gaattgacag cacaccgccg
     1981 tcgaggcaag cttgagcggg gtgcactcat catagtgcag gaaagaagct ctacatattc
     2041 aggaggattc accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     2101 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     2161 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     2221 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     2281 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     2341 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     2401 gcacttcgtg gccgaggagc aggacgacta caaggacgac gacgacaagg ccgccaacga
     2461 cgagaactac tcggagaact acgccgacgc ctcgtagctg cagccatgtc agccgttaag
     2521 tgttcctgtg tcactgaaaa ttgctttgag aggctctaag ggcttctcag tgcgttacat
     2581 ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt atatattctt
     2641 ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt atattaattt
     2701 tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg ttagccatga
     2761 gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt agtacgttaa
     2821 acatgagagc ttagtacgtg aaacatgaga gcttagtacg tactatcaac aggttgaact
     2881 gctggatcct tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2941 ctgatgttac attgcacaag ataaaataat atcatcatga acaataaaac tgtctgctta
     3001 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     3061 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     3121 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     3181 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     3241 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     3301 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     3361 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     3421 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     3481 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     3541 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     3601 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     3661 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     3721 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     3781 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcag
//
