LOCUS       pJW168.        5728 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector allowing inducible expression of Cre recombinase 
            for in vivo site-specific recombination.
ACCESSION   .
VERSION     .
KEYWORDS    pJW168
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The temperature-sensitive pSC101 replicon enables pJW168 to be
FEATURES             Location/Qualifiers
     source          1..5728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(7..1035)
                     /label=Cre
                     /note="site-specific recombinase"
     protein_bind    1084..1100
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1108..1138)
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(1179..1195)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1203..1233)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     promoter        1441..1518
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             1519..2598
                     /label=lacI
                     /note="lac repressor"
     protein_bind    2614..2635
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2755..2859
                     /label=AmpR promoter
     CDS             2860..3717
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4342..4564
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             4612..5559
                     /label=Rep101(Ts)
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
ORIGIN
        1 ttactaatcg ccatcttcca gcaggcgcac cattgcccct gtttcactat ccaggttacg
       61 gatatagttc atgacaatat ttacattggt ccagccacca gcttgcatga tctccggtat
      121 tgaaactcca gcgcgggcca tatctcgcgc ggctccgaca cgggcactgt gtccagacca
      181 ggccaggtat ctctgaccag agtcatcctt agcgccgtaa atcaatcgat gagttgcttc
      241 aaaaatccct tccagggcgc gagttgatag ctggctggtg gcagatggcg cggcaacacc
      301 attttttctg acccggcaaa acaggtagtt attcggatca tcagctacac cagagacgga
      361 aatccatcgc tcgaccagtt tagttacccc caggctaagt gccttctcta cacctgcggt
      421 gctaaccagc gttttcgttc tgccaatatg gattaacatt ctcccaccgt cagtacgtga
      481 gatatcttta accctgatcc tggcaatttc ggctatacgt aacagggtgt tataagcaat
      541 ccccagaaat gccagattac gtatatcctg gcagcgatcg ctattttcca tgagtgaacg
      601 aacctggtcg aaatcagtgc gttcgaacgc tagagcctgt tttgcacgtt caccggcatc
      661 aacgttttct tttcggatcc gccgcataac cagtgaaaca gcattgctgt cacttggtcg
      721 tggcagcccg gaccgacgat gaagcatgtt tagctggccc aaatgttgct ggatagtttt
      781 tactgccaga ccgcgcgcct gaagatatag aagataatcg cgaacatctt caggttctgc
      841 gggaaaccat ttccggttat tcaacttgca ccatgccgcc cacgaccggc aaacggacag
      901 aagcattttc caggtatgct cagaaaacgc ctggcgatcc ctgaacatgt ccatcaggtt
      961 cttgcgaacc tcatcactcg ttgcatcgac cggtaatgca ggcaaatttt ggtgtacggg
     1021 cagtaaattg gacatgtcaa cggtacctgc agtctagagt cgaggcctgt ttcctgtgtg
     1081 aaattgttat ccgctcacaa ttccacacat tatacgagcc ggaagcataa agtgtaaagc
     1141 ctggggtgcc taatgagtga gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     1201 cacattatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctgc
     1261 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     1321 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     1381 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagacg gagtgtatcc
     1441 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
     1501 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
     1561 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
     1621 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
     1681 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
     1741 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
     1801 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
     1861 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
     1921 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
     1981 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
     2041 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
     2101 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
     2161 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
     2221 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
     2281 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
     2341 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
     2401 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     2461 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     2521 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2581 cgactggaaa gcgggcagtg agcgcaacgc aatcaatgtg agttagctca ctcattaggc
     2641 accccaggct ttacacttta tgcttccgac catactggct taactatgcg gcatcagagc
     2701 agattgtact gagagtgcac catcgatgca ggtggcactt ttcggggaaa tgtgcgcgga
     2761 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2821 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2881 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2941 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3001 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3061 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3121 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3181 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3241 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3301 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3361 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3421 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3481 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3541 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3601 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3661 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3721 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3781 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3841 ttttcgttcc actgagcgtc agaccccgtt gatgataccg ctgccttact gggtgcatta
     3901 gccagtctga atgacctgtc acgggataat ccgaagtggt cagactggaa aatcagaggg
     3961 caggaactgc tgaacagcaa aaagtcagat agcaccacat agcagacccg ccataaaacg
     4021 ccctgagaag cccgtgacgg gcttttcttg tattatgggt agtttccttg catgaatcca
     4081 taaaaggcgc ctgtagtgcc atttaccccc attcactgcc agagccgtga gcgcagcgaa
     4141 ctgaatgtca cgaaaaagac agcgactcag gtgcctgatg gtcggagaca aaaggaatat
     4201 tcagcgattt gcccgattgc ggccgcaacc gagcttgcga gggtgctact taagccttta
     4261 gggttttaag gtctgttttg tagaggagca aacagcgttt gcgacatcct tttgtaatac
     4321 tgcggaactg actaaagtag tgagttatac acagggctgg gatctattct ttttatcttt
     4381 ttttattctt tctttattct ataaattata accacttgaa tataaacaaa aaaaacacac
     4441 aaaggtctag cggaatttac agagggtcta gcagaattta caagttttcc agcaaaggtc
     4501 tagcagaatt tacagatacc cacaactcaa aggaaaagga ctagtaatta tcattgacta
     4561 gcccatctca attggtatag tgattaaaat cacctagacc aattgagatg tatgtctgaa
     4621 ttagttgttt tcaaagcaaa tgaactagcg attagtcgct atgacttaac ggagcatgaa
     4681 accaagctaa ttttatgctg tgtggcacta ctcaacccca cgattgaaaa ccctacaagg
     4741 aaagaacgga cggtatcgtt cacttataac caatacgttc agatgatgaa catcagtagg
     4801 gaaaatgctt atggtgtatt agctaaagca accagagagc tgatgacgag aactgtggaa
     4861 atcaggaatc ctttggttaa aggctttgag attttccagt ggacaaacta tgccaagttc
     4921 tcaagcgaaa aattagaatt agtttttagt gaagagatat tgccttatct tttccagtta
     4981 aaaaaattca taaaatataa tctggaacat gttaagtctt ttgaaaacaa atactctatg
     5041 aggatttatg agtggttatt aaaagaacta acacaaaaga aaactcacaa ggcaaatata
     5101 gagattagcc ttgatgaatt taagttcatg ttaatgcttg aaaataacta ccatgagttt
     5161 aaaaggctta accaatgggt tttgaaacca ataagtaaag atttaaacac ttacagcaat
     5221 atgaaattgg tggttgataa gcgaggccgc ccgactgata cgttgatttt ccaagttgaa
     5281 ctagatagac aaatggatct cgtaaccgaa cttgagaaca accagataaa aatgaatggt
     5341 gacaaaatac caacaaccat tacatcagat tcctacctac ataacggact aagaaaaaca
     5401 ctacacgatg ctttaactgc aaaaattcag ctcaccagtt ttgaggcaaa atttttgagt
     5461 gacatgcaaa gtaagyatga tctcaatggt tcgttctcat ggctcacgca aaaacaacga
     5521 accacactag agaacatact ggctaaatac ggaaggatct gaggttctta tggctcttgt
     5581 atctatcagt gaagcatcaa gactaacaaa caaaagtaga acaactgttc accgttacat
     5641 atcaaaggga aaactgtcca tacccatggg ctagctgatc agccagtgcc aagcttgctc
     5701 aatcaatcac cggatccccc gggaattc
//
