LOCUS       Exported                8801 bp DNA     circular SYN 22-JAN-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   van Kessel JC, Hatfull GF
  TITLE     Recombineering in Mycobacterium tuberculosis.
  JOURNAL   Nat Methods. 2007 Feb . 4(2):147-52.
  PUBMED    17179933
REFERENCE   2  (bases 1 to 8801)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8801)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8801)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Feb . 4(2):147-52."
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..1884
                     /mol_type="other DNA"
                     /clone="pJV53-Cpf1"
                     /note="derived from Francisella tularensis subsp.
                     novicida;
                     codon-optimized FnCpf1 inducing expression vector"
                     /db_xref="taxon:32630"
                     /organism="synthetic construct"
     source          1885..8801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3110..3538
                     /mol_type="other DNA"
                     /label=Escherichia coli/Mycobacterium shuttle plasmid
                     /note="Escherichia coli/Mycobacterium shuttle plasmid"
                     /db_xref="taxon:930172"
                     /organism="Shuttle vector pMycoFos"
     source          3620..3904
                     /mol_type="other DNA"
                     /label=Escherichia coli/Mycobacterium shuttle plasmid
                     /note="Escherichia coli/Mycobacterium shuttle plasmid"
                     /db_xref="taxon:930172"
                     /organism="Shuttle vector pMycoFos"
     source          3909..4550
                     /mol_type="other DNA"
                     /label=Escherichia coli/Mycobacterium shuttle plasmid
                     /note="Escherichia coli/Mycobacterium shuttle plasmid"
                     /db_xref="taxon:930172"
                     /organism="Shuttle vector pMycoFos"
     rep_origin      1..1884
                     /label=oriM
                     /note="oriM"
     CDS             complement(1934..3007)
                     /codon_start=1
                     /label=amiC
                     /translation="MQDGEVEFRVGLVIPLQGPAGIFAPSCEAVAELAAKEVNDRGGLQ
                     GRKVTIEVLDGGAPGDDVARTVADRLRGHGLDAVTGWHISAVRNRISPVVRDRIPYVYT
                     SLYEGGERTPGVFCTGETPQIQIAPALAWLRDHFGIRSWCLVGDDYIWPRRSAAAARAY
                     CRDLDLELRREIYVPYGTDDFRAPVRKAIASGAQAVLMLLVGQDAVLFNREFARAGGHD
                     RMARFSPLMEENMLLASGAGSTENLYVAAAYFSSLATAGAMDLMGSYVARYGADAPPLN
                     AMAESCYEGLLALEAIFQRAHSPEIPDLMASAHDVGFDGPRGPMCMRDSQFDQQVYIAS
                     ADGYDFDILDTLATLDA"
     promoter        3045..4568
                     /label=Acetamidase Promoter
     gene            3110..3538
                     /gene="amiA"
                     /label=amiA
     CDS             3110..3538
                     /codon_start=1
                     /transl_table=11
                     /gene="amiA"
                     /product="AmiA"
                     /label=amiA
                     /note="acetamidase regulator"
                     /protein_id="ADR02800.1"
                     /translation="MAAGQQRRPNLLLPLVRLTHLAESAIERVLADSSLKIEDWRVLDE
                     LAGRRTVPMSDLAQATLITGPTLTRTVDRLVSQGIIYRTADLHDRRRVLVALTPRGRTL
                     RNRLVDAVAEAECAAFESCGLDVDQLRELVDTTSNLTS"
     promoter        3558..3603
                     /label=P2 promoter
     gene            3620..3904
                     /gene="amiD"
                     /label=amiD
     CDS             3620..3904
                     /codon_start=1
                     /transl_table=11
                     /gene="amiD"
                     /product="AmiD"
                     /label=amiD
                     /note="acetamidase regulator"
                     /protein_id="ADR02801.1"
                     /translation="MPTYTFRCSHCGPFDLTCAISERDAAATCPECRTPARRVFGSVGL
                     TTFTAGHHRAFDAASASAESPTVVKSIPAGADRPRAPRRNPGLPSLPRY"
     gene            3909..4550
                     /gene="amiS"
                     /label=amiS
     CDS             3909..4550
                     /codon_start=1
                     /transl_table=11
                     /gene="amiS"
                     /product="AmiS"
                     /label=amiS
                     /note="acetamidase transporter"
                     /protein_id="ADR02802.1"
                     /translation="MGGVGLFYVGAVLIIDGLMLLGRISPRGATPLNFFVGGLQVVTPT
                     VLILQSGGDAAVIFAASGLYLFGFTYLWVAINNVTDWDGEGLGWFSLFVAIAALGYSWH
                     AFTAEADPAFGVIWLLWAVLWFMLFLLLGLGHDALGPAVGFVAVAEGVITAAVPAFLIV
                     SGNWETGPLPAAVIAVIGFAAVVLAYPIGRRLAAPSVTNPPPAALAATTR"
     CDS             4639..5583
                     /codon_start=1
                     /label=che9c 60
                     /note="exonuclease VIII from Mycobacterium phage Che9c"
                     /translation="MSVPTQDGMHRFVDEDVYHADRGSLSVSGAKLLLPPSCPAKFRWE
                     MDNTRKPKKVWDFGHVAHKLVLGKGAEFEILDPEVHGLKADGTPSEKPTATGMWRKAEA
                     EARKQGKVPIHVDLFTKAYDMAEKVRQHPTAGPIFANPDGEAEVALYYTDPETGVRLRG
                     RIDWLTDDIDDYKTSMTANPAELKTKFYKLGYFMQAAWYIDLLVALGLAENPRFRFITQ
                     EKEPPYVVTPIQYDDEAIEEGRRRNRQAIRLYADCMESGKWPDYSDDVVTISLPSWGLP
                     RPQTVGDVVTDSYIYDTDPLEEADPIEGDYIYG"
     CDS             5576..6637
                     /codon_start=1
                     /label=che9c 61
                     /note="recombinase RecT from Mycobacterium tuberculosis"
                     /translation="MAENAVTKQDSPKAPETISQVLQVLVPQLARAVPKGMDPDRIARI
                     VQTEIRKSRNAKAAGIAKQSLDDCTQESFAGALLTSAALGLEPGVNGECYLVPYRDTRR
                     GVVECQLIIGYQGIVKLFWQHPRASRIDAQWVGANDEFHYTMGLNPTLKHVKAKGDRGN
                     PVYFYAIVEVTGAEPLWDVFTADEIRELRRGKVGSSGDIKDPQRWMERKTALKQVLKLA
                     PKTTRLDAAIRADDRPGTDLSQSQALALPSTVKPTADYIDGEIAEPHEVDTPPKSSRAQ
                     RAQRATAPAPDVQMANPDQLKRLGEIQKAEKYNDADWFKFLADSAGVKATRAADLTFDE
                     AKAVIDMFDGPNA"
     terminator      6702..6748
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             6909..7721
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      8055..8643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtgagcccac cagctccgta agttcgggcg ctgtgtggct cgtacccgcg cattcaggcg
       61 gcagggggtc taacgggtct aaggcggcgt gtacggccgc cacagcggct ctcagcggcc
      121 cggaaacgtc ctcgaaacga cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg
      181 ctcggctgtc gctggtgttc caccaccagg gctcgacggg agagcggggg agtgtgcagt
      241 tgtggggtgg cccctcagcg aaatatctga cttggagctc gtgtcggacc atacaccggt
      301 gattaatcgt ggtctactac caagcgtgag ccacgtcgcc gacgaatttg agcagctctg
      361 gctgccgtac tggccgctgg caagcgacga tctgctcgag gggatctacc gccaaagccg
      421 cgcgtcggcc ctaggccgcc ggtacatcga ggcgaaccca acagcgctgg caaacctgct
      481 ggtcgtggac gtagaccatc cagacgcagc gctccgagcg ctcagcgccc gggggtccca
      541 tccgctgccc aacgcgatcg tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc
      601 actcaacgcc cctgttccac gcaccgaata cgcgcggcgt aagccgctcg catacatggc
      661 ggcgtgcgcc gaaggccttc ggcgcgccgt cgatggcgac cgcagttact caggcctcat
      721 gaccaaaaac cccggccaca tcgcctggga aacggaatgg ctccactcag atctctacac
      781 actcagccac atcgaggccg agctcggcgc gaacatgcca ccgccgcgct ggcgtcagca
      841 gaccacgtac aaagcggctc cgacgccgct agggcggaat tgcgcactgt tcgattccgt
      901 caggttgtgg gcctatcgtc ccgccctcat gcggatctac ctgccgaccc ggaacgtgga
      961 cggactcggc cgcgcgatct atgccgagtg ccacgcgcga aacgccgaat ttccgtgcaa
     1021 cgacgtgtgt cccggaccgc taccggacag cgaggtccgc gccatcgcca acagcatttg
     1081 gcgttggatc acaaccaagt cgcgcatttg ggcggacggg atcgtggtct acgaggccac
     1141 actcagtgcg cgccagtcgg ccatctcgcg gaagggcgca gcagcgcgca cggcggcgag
     1201 cacagttgcg cggcgcgcaa agtccgcgtc agccatggag gcattgctat gagcgacggc
     1261 tacagcgacg gctacaaccg gcagccgact gtccgcaaaa agcggcgcgt gaccgccgcc
     1321 gaaggcgctc gaatcaccgg actatccgaa cgccacgtcg tccggctcgt ggcgcaggaa
     1381 cgcagcgagt ggctcgccga gcaggctgca cgccgcgaac gcatccgcgc ctatcacgac
     1441 gacgagggcc actcttggcc gcaaacggcc aaacatttcg ggctgcatct ggacaccgtt
     1501 aagcgactcg gctatcgggc gaggaaagag cgtgcggcag aacaggaagc ggctcaaaag
     1561 gcccacaacg aagccgacaa tccaccgctg ttctaacgca attggggagc gggtgtcgcg
     1621 ggggttccgt ggggggttcc gttgcaacgg gtcggacagg taaaagtcct ggtagacgct
     1681 agttttctgg tttgggccat gcctgtctcg ttgcgtgttt cgttgcgccc gttttgaata
     1741 ccagccagac gagacggggt tctacgaatc ttggtcgata ccaagccatt tccgctgaat
     1801 atcggggagc tcaccgccag aatcggtggt tgtggtgatg tacgtggcga actccgttgt
     1861 agtgcctgtg gtggcatccg tggcgcggcc gcggtaccag atctttaaat ctagataaag
     1921 aagtgacgcg gtctcaagcg tcgagcgtcg ccagcgtgtc gaggatgtcg aagtcgtagc
     1981 cgtcggcgct ggcgatgtag acctgctggt cgaattgact gtcgcgcata cacatcgggc
     2041 cccggggccc gtcgaacccg acatcgtgcg cggatgccat caggtccggt atctcggggg
     2101 agtgggcccg ctggaagatg gcctcgagcg caagcagacc ctcgtaacag gattcggcca
     2161 tcgcgttgag cggtggcgcg tcggcgccgt agcgggcgac gtagctgccc atcaggtcca
     2221 tggcacccgc ggtggccagt gaactgaagt acgccgcggc gacatagagg ttttcggtgg
     2281 agccggcgcc gctggccagc agcatgttct cctccatcag cgggctgaac cgcgccatgc
     2341 ggtcgtgccc gccggcgcgc gcgaactcgc ggttgaacaa cacggcgtcc tggccgacga
     2401 gcagcatcaa cacggcctgc gcccccgacg cgatggcctt gcggacaggt gcgcggaaat
     2461 cgtcggtgcc gtacgggacg tagatctccc gtctgagctc gaggtccaga tctcggcagt
     2521 acgcgcgggc ggccgcggcg gaacggcgcg gccagatgta gtcatcgccg accaggcacc
     2581 aggaccggat gccgaagtgg tcgcgcagcc aggcgagcgc gggcgcgatc tggatctgcg
     2641 gtgtctcgcc tgtgcagaac acgcccggtg tgcgttcacc gccctcgtac aacgaggtgt
     2701 agacgtacgg gatgcggtcg cggaccaccg gggagatgcg gttgcgcacg gccgagatgt
     2761 gccagccggt cacggcgtcg agaccgtgac ctcgcaaccg gtcggcgacg gtccgggcga
     2821 cgtcgtcgcc gggcgctccg ccgtcgagca cctcgatggt gaccttgcgg ccctgcaggc
     2881 cgcctcggtc gttgacctcc ttggccgcga gctcggccac ggcctcgcac gaaggcgcga
     2941 agattcccgc tggcccttga agcggaatca ccagcccgac gcggaactca acctcgccgt
     3001 cctgcactcc agatcaccgt cgatcccgtg tagtctgcgc ttcaaagctt tctagcagaa
     3061 ataattcatt ctgaacagac cccgccgtcg acacgaggag acacccacca tggccgccgg
     3121 acagcagcgc cgccccaacc tcctgctgcc gttggtgcgt ctgacccacc tcgcggagtc
     3181 ggcgatcgaa cgcgtgctcg cggactcgtc gctcaagatc gaggactggc gcgtgctcga
     3241 cgagttggcc ggacggcgca ccgtgcccat gagcgatctc gcgcaggcca cgctgatcac
     3301 gggtccgact ctcaccagaa ccgtcgatcg ccttgtgtcg caagggatca tctaccggac
     3361 tgccgatctg catgaccgcc ggcgggtgct cgtggcgttg accccgcggg ggcggacgct
     3421 gcgcaaccgc ctggtggacg cggtagccga ggccgagtgt gcggcttttg aatcgtgcgg
     3481 gctggacgtc gaccagttgc gcgaactcgt cgacaccacc tcgaatttga cttcgtaacc
     3541 acccgcgccc ggcgcgggcg ttcacccttg acttttattt tcatctggat atatttcggg
     3601 tgaatggaaa ggggtgacca tgccgaccta cacattccgt tgttcccact gcggtccctt
     3661 cgatctcacc tgcgcgatct ccgagcgcga tgcggcggcg acctgtccgg agtgccggac
     3721 gccggcgcgc cgggtcttcg gttcggtagg gctgacgaca ttcaccgcgg gacatcaccg
     3781 cgcattcgac gcggcgtccg cgagcgccga aagtcccacg gtggtgaagt cgattcccgc
     3841 aggcgcggac cgcccgcggg ccccgcgccg caatcccggt ctaccgagtc tgccgaggta
     3901 ctagcgacat gggtggcgtc gggctcttct acgtgggtgc ggtgctcatc atcgacgggc
     3961 tgatgctgct gggccgcatc agcccacgag gcgcaacacc gctgaacttc ttcgtcggcg
     4021 gactgcaggt ggtgacgcct acggtgctga tcctgcagtc cggcggagac gcggccgtga
     4081 tcttcgcggc ctccgggctc tacctgttcg gcttcaccta cctgtgggtg gccatcaaca
     4141 acgtgaccga ctgggacgga gaaggtctcg gatggttctc gctgttcgtc gcgatcgccg
     4201 cactcggcta ctcgtggcac gcgttcaccg ccgaggccga cccggcgttc ggggtgatct
     4261 ggctgctgtg ggcagtgctg tggttcatgc tgttcctgct gctcggcctg gggcacgacg
     4321 cactggggcc cgccgtcggg ttcgtcgcgg tggccgaagg cgtgatcacc gccgccgtgc
     4381 cggccttcct gatcgtgtcg ggcaactggg aaaccggccc gctccccgcc gcggtcatcg
     4441 ccgtgatcgg ttttgccgca gttgttctcg cataccccat cgggcgccgt ctcgcagcgc
     4501 cgtcagtcac caaccctcca ccggccgcgc tcgcggccac cacccgataa gagaaaggga
     4561 gtccacatat gtaacggatc cagctgcaga attcgaagct tatcgatgtc gacgtagtta
     4621 cgagatcggc ggccgcatat gagtgtgccc acacaggacg gaatgcaccg gttcgtcgac
     4681 gaggacgtct accacgctga ccggggctcg ctgtcggtat ccggcgcgaa gctgctgttg
     4741 ccgccgtcgt gtcccgcgaa attccgctgg gagatggaca acacccggaa gccgaaaaag
     4801 gtctgggact tcggacatgt cgcgcacaaa ctggtgctcg gcaagggtgc cgagttcgag
     4861 atcctcgacc ccgaggtgca cgggctgaag gcggacggta cgccgtcgga gaagccgacc
     4921 gcgacgggca tgtggcgcaa ggccgaggct gaggctcgca aacagggcaa ggtgccgatt
     4981 cacgtcgacc tgttcacgaa ggcgtacgac atggccgaaa aggtgcgtca gcacccgaca
     5041 gccggcccga tcttcgccaa tcctgacggc gaggccgagg tcgcgctgta ctacaccgac
     5101 cccgagaccg gcgtgcggct gcgtggccgg atcgactggc tcactgacga tatcgatgat
     5161 tacaagacgt cgatgaccgc gaacccggcc gagctgaaaa ccaagttcta caagctcggc
     5221 tatttcatgc aggcggcctg gtacatcgat ctactggtcg ccctcgggct cgccgagaac
     5281 ccgcgattcc ggttcatcac gcaggagaaa gaaccgccct acgtcgtgac tccgatccag
     5341 tacgacgacg aggcgatcga agaggggcgg cgccgcaacc gccaggcgat ccggctctac
     5401 gccgactgca tggaatcggg caagtggcct gactacagcg acgacgtggt cacgatcagc
     5461 ctgccctcgt gggggctgcc gcgaccgcag accgtcggcg acgtcgtcac cgacagctat
     5521 atctacgaca ccgacccgct cgaagaggcc gacccgattg aaggggatta catctatggc
     5581 tgaaaatgct gtcaccaagc aggattcgcc caaggcaccc gagacgatct cgcaagtgct
     5641 gcaggtgctc gtgccgcagc tggcgcgtgc agtgcccaag ggtatggacc ccgaccgcat
     5701 cgcgcggatc gtgcaaaccg agatccgcaa gtcgcgcaac gcgaaagctg ctggcatcgc
     5761 taagcagtcc ctcgacgact gcacgcaaga gtcatttgcc ggtgcgctgc tgacctcggc
     5821 cgcgctcggt ctcgagcccg gtgtcaacgg cgagtgctac ctcgtgccct accgcgacac
     5881 ccggcgcggc gtggtcgagt gccagctgat catcggctac cagggcatcg tcaaactgtt
     5941 ctggcagcac ccgcgcgcct cccggatcga cgcgcagtgg gtcggcgcga acgacgaatt
     6001 ccattacacc atgggcctca atccgacgct gaaacacgtg aaggccaagg gtgatcgggg
     6061 taatccggtc tacttctacg cgatcgtcga ggtgaccggc gctgagccgc tgtgggacgt
     6121 gttcaccgcc gacgagatca gggaattgcg tcgcggcaag gtcggatcct cgggcgacat
     6181 caaggacccg cagcgctgga tggagcggaa gaccgcgctc aaacaggtgc tgaagctggc
     6241 accgaagacg acgcggctcg acgcggcgat ccgcgccgac gatcgcccgg gcaccgacct
     6301 gtcacagtcg caggcgctcg cgctgccgtc gaccgtcaag ccgacggccg actacatcga
     6361 cggcgagatc gccgagccgc acgaggtcga tacgccaccg aagtcgtcgc gcgcacaacg
     6421 cgcgcagcgc gccaccgcgc cggcgcccga cgtgcagatg gccaatcccg atcagctgaa
     6481 gcgcctcggc gagatccaga aggccgaaaa gtacaacgac gccgattggt tcaagttcct
     6541 cgccgattcg gccggcgtca aagccacgcg cgccgccgac ctcacgttcg acgaggcgaa
     6601 ggctgtcatc gacatgttcg acgggcccaa cgcatgagcg ccccggcgaa ttccgacgcg
     6661 gtggttgatc tgcaactagc gtacgatcga ctgccaggca tcaaataaaa cgaaaggctc
     6721 agtcgaaaga ctgggccttt cgttttatgc catcatggcc gcggtgatca gctagccacc
     6781 tgacgtcngg ggggggggaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac
     6841 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     6901 ggggtgttat gagccatatt caacgggaaa cgtcttgctc gaggccgcga ttaaattcca
     6961 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     7021 cgacaatcta tcgcttgtat gggaagcccc atgcgccaga gttgtttctg aaacatggca
     7081 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     7141 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     7201 ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     7261 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     7321 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     7381 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     7441 tctggaaaga aatgcataat cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     7501 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     7561 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     7621 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     7681 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     7741 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcggcttt gttgaataaa
     7801 tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg cagaccgttc
     7861 cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag ctctcaccaa
     7921 ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcctggt atgagtcagc
     7981 aacaccttct tcacgaggca gacctcacta gttccatgag cgtcagaccc cgtagaaaag
     8041 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8101 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8161 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     8221 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8281 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8341 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8401 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8461 acgcttcccg aggggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8521 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8581 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8641 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8701 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8761 gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg c
//
