LOCUS       V012939                 9107 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012939
VERSION     V012939
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             432..1238
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     oriT            1573..1682
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             1715..2083
                     /label="traJ"
                     /note="oriT-recognizing protein"
     protein_bind    3457..3478
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3493..3523
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3531..3547
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3555..3571
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3592..3610
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    3623..3730
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(3739..3757)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3767..3783)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4030..4134
                     /label="AmpR promoter"
     CDS             4135..4992
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5166..5754
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(6139..6273)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttgggggggc aggagccccg cggggggtgg tcgggtcagg cgccgaaccg gcgggcggcg
       61 gcggcgacca ggccgtcggc ggcggccatg gcgcggtcgc ggtcggtggt gagggcggtg
      121 cggtcggcgg cggcccagtc ctgctctcac gcaacgtcta cgtggacgct cagggcgaca
      181 cgatcgaggt cggggagtcc gtcgctccgg cgggccgctg gcgcgtccac cgggactgat
      241 caaggcgaat acttcatatg cggggatcga ccgcgcgggt cccggacggg gaagagcggg
      301 gagctttgcc agagagcgac gacttcccct tgcgttggtg attgccggtc agggcagcca
      361 tccgccatcg tcgcgtaggg tgtcacaccc caggaatcgc gtcactgaac acagcagccg
      421 gtaggacgac catgactgag ttggacacca tcgcaaatcc gtccgatccc gcggtgcagc
      481 ggatcatcga tgtcaccaag ccgtcgcgat ccaacataaa gacaacgttg atcgaggacg
      541 tcgagcccct catgcacagc atcgcggccg gggtggagtt catcgaggtc tacggcagcg
      601 acagcagtcc ttttccatct gagttgctgg atctgtgcgg gcggcagaac ataccggtcc
      661 gcctcatcga ctcctcgatc gtcaaccagt tgttcaaggg ggagcggaag gccaagacat
      721 tcggcatcgc ccgcgtccct cgcccggcca ggttcggcga tatcgcgagc cggcgtgggg
      781 acgtcgtcgt tctcgacggg gtgaagatcg tcgggaacat cggcgcgata gtacgcacgt
      841 cgctcgcgct cggagcgtcg gggatcatcc tggtcgacag tgacatcacc agcatcgcgg
      901 accggcgtct ccaaagggcc agccgaggtt acgtcttctc ccttcccgtc gttctctccg
      961 gtcgcgagga ggccatcgcc ttcattcggg acagcggtat gcagctgatg acgctcaagg
     1021 cggatggcga catttccgtg aaggaactcg gggacaatcc ggatcggctg gccttgctgt
     1081 tcggcagcga aaagggtggg ccttccgacc tgttcgagga ggcgtcttcc gcctcggttt
     1141 ccatccccat gatgagccag accgagtctc tcaacgtttc cgtttccctc ggaatcgcgc
     1201 tgcacgagag gatcgacagg aatctcgcgg ccaaccgata agcgcctctg ttcctcggac
     1261 gctcggttcc tcgacctcga ttcgtcagtg atgatcagac gtcgaaggtg tagtgccggg
     1321 tgtggtccag cagggccgcc ggggtggtgt cgttccacgg cttcatcgtc tcgttgaggt
     1381 cgaccaaagc ggccatcgtg cctccccact cctgcagatc cctgcaggtc gacggatctt
     1441 ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt
     1501 tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca
     1561 acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt
     1621 cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc
     1681 ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag
     1741 gaagggcagc ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga
     1801 aaaggcggcg gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta
     1861 caaaatcacg ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga
     1921 cctgggccgc ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg
     1981 gttcggtgat gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct
     2041 tggcaaggtc atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct
     2101 aaaacggccg gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga
     2161 gcgacttcgc ggagctggtg aagtacatca ccgacgagca aggcaagacc gatccccggg
     2221 gacctgcagg tcgactctag ctagacacgt ctgaagctag cttcgaggaa ctttcggcgg
     2281 ctttgctgtg cgacaggctc acgtctaaaa ggaaataaat catgggtcat aaaattatca
     2341 cgttgtccgg cgcggcgacg gatgttctgt atgcgctgtt tttccgtggc gcgttgctgt
     2401 ctggtgatct gccttctaaa tctggcacag ccgaattgcg cgagcttggt tttgctgaaa
     2461 ccagacacac agcaactgaa taccagaaag aaaatcactt tacctttctg acatcagaag
     2521 ggcagaaatt tgccgttgaa cacctggtca atacgcgttt tggtgagcag caatattgcg
     2581 cttcgatgac gcttggcgtt gagattgata cctctgctgc acaaaaggca atcgacgagc
     2641 tggaccagcg cattcgtgac accgtctcct tcgaacttat tcgcaatgga gtgtcattca
     2701 tcaaggacgc cgctatcgca aatggtgcta tccacgcagc ggcaatcgaa acacctcagc
     2761 cggtgaccaa tatctacaac atcagccttg gtatccagcg tgatgagcca gcgcagaaca
     2821 aggtaaccgt cagtgccgat aagttcaaag ttaaacctgg tgttgatacc aacattgaaa
     2881 cgttgatcga aaacgcgctg aaaaacgctg ctgaatgtgc ggcgctggat gtcacaaagc
     2941 aaatggcagc agacaagaaa gcgatggatg aactggcttc ctatgtccgc acggccatca
     3001 tgatggaatg tttccccggt ggtgttatct ggcagcagtg ccgtcgatag tatgcaattg
     3061 ataattatta tcatttgcgg gtcctttccg gcgatccgcc ttgttacggg gcggcgacct
     3121 cgcgggtttt cgctatttat gaaaattttc cggtttaagg cgtttccgtt cttcttcgtc
     3181 ataacttaat gtttttattt aaaataccct ctgaaaagaa aggaaacgac aggtgctgaa
     3241 agcgagcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg
     3301 gaagtcaaca aaaagcagag cttatcgatg ataagcggtc aaacatgaga attgcccaat
     3361 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     3421 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     3481 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     3541 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     3601 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaactagtgg
     3661 atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg
     3721 gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     3781 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3841 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3901 tgcgcagcct gaatggcgaa tggcaattct tgaagacgaa agggcctcgt gatacgccta
     3961 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     4021 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4081 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4141 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4201 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4261 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4321 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     4381 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4441 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4501 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4561 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4621 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     4681 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4741 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4801 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4861 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4921 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4981 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5041 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5101 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5161 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5221 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5281 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5341 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5401 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5461 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5521 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5581 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5641 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5701 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5761 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5821 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5881 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     5941 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     6001 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     6061 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     6121 gatcatgcgc acccgtcaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     6181 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     6241 tttgtaacca ttataagctg caataaacaa gttcccgcac gatcagcgtc ccgccgacca
     6301 ggagcagtgc ggccagcact gccgctaacg cctggtcctg gtcccggtcc tggtggtgca
     6361 tcagtcctcc ccgtgatcac ttcggcaccc accgtagtga tcacccccga cagcggatca
     6421 aggggtttgc gggtcccggt cggcgccggg cgggggaggc aggagccgcc gacgctgcct
     6481 ctgggacggg ccggacggca gggggaccgg cggccgggcg agctgcagcc gggggtccgg
     6541 cagggccgga gcgggcggaa ccgtgctctg acctgcggcc cgagtttcgt cacgtgacgg
     6601 aatggaaggc tgctgcattt cgtcacgtga cgtatctcgg cgagcgactg ccgacgccac
     6661 ggcggacacg atcgcctcgc gctggcgccg ggcctcgtac gcccgctggc ggcaggagcg
     6721 gcggcagtag tcccggctcc ggccgacgcc ggattgcttg atctccgagc cgcaccaggc
     6781 gcagagcttc gcgccgtcgg cgtccctggg ggtggtggtg ctcatggccg acgaccgtac
     6841 gcggcacgtc tcgtagcgag gcgagtcggg cgcgacgcct gcacgaagtg ccggcggggc
     6901 cgaccccggg cgagtaatcc caggattact cccgcggctt cgaccccggc cgccgtcgcc
     6961 gcgtacgtca ccgacccccg ccgtacgtca ccgggatgac gtacggcggg ggggagcgag
     7021 ttagtgcgaa gtgggcccac ttgcgagccg ggcgatgtgc cgggcggccc gctcctggcg
     7081 gtcgtcggcg tcgtcgtcct ggtcgtcgtc ctgctctcgc cgtcggcgtg cagttgcttc
     7141 ctcgcggcgc tgggcgaggg cggcgagcat gtcggcgtac gcctcggcca cctcccccgc
     7201 cgtgagcacc accactgtgt cggccgcgtc ggccagcgcc aggacctccc gcacccgttc
     7261 gcccacggcc gccgaatcct cgttgccgtc cttgccttcg gcggcccggg tcgcctcgag
     7321 gtcgagggcg cggcgggtga ccgcgtgcca tccgtcctcg gtcacggcga ccccggcccg
     7381 cagctccccg ccgtcggcgt cggccgccag gagcagatcg aggtcgtcgg cctcggtgtc
     7441 gccgccgtcg agcccgagca tctgccgcag gtagcgggtc cattcgatgg cccggcgtcc
     7501 ccgggttgcc cgctcgtact cgtgccagcg cgagaggttc cactccagcg agccgacccc
     7561 ggcggcgtcg tcctcggtca tgccgccggt caggtccccg atccgtccga ggagttcgaa
     7621 cggggcgacg ttcccgccgg tcgccgtctt gaggtcggcg cgggcgagtt cgagggcggg
     7681 cgccttcccg tcctgggtct tggcgatgta ctcggcgagg tcgttggcgt cgcgctcggt
     7741 ctccagccgc ttgaagtcga cgccgtgccg gtcgtcgggc gtgaaggcgg ggttgacctt
     7801 gcgcagggcg gcggtccaca cggaccgcca gtgcccctgc cactcgtcga gcgcggcgcc
     7861 ggtcggctcg aaggtggcga cgatctgctt cgcggaccgc tccccctcgg tccggccgcc
     7921 gaccaggacg atcgcgtgga tgtgcgggtg ccagccgttg atctgcccca cggtgacttc
     7981 ggtcgcgcgg atcatgccga cgtacccgat ccggtctcgg atgccctcgc ggtcggcggc
     8041 ccggtgcccg tccttggccc ggcgtccggc ccacgtgccg cccgtgatca gtcgctggta
     8101 ggcgcccggc cgccgggggc tgtccggcgt cttccgggtg ccctggaggg cgtccatgag
     8161 gtccgcgagc cggtccgtgt gcccatggcg ggccgtgaag gtgaccaggt aggcggtccc
     8221 cccgcgcttg atccactcga ccacggcggc ggtgatctcc tcggcccgct tgtgccggat
     8281 cgtggcggcg cagaccgggc agagccagat ccgcccgcac cgcatcaggc ccaggaccac
     8341 ggacgttccg gccgccgtct gggcgacgat cacgccggag gcagggtcca tcagggcgcg
     8401 gccgcagccc ttgcacgcgg cgtccccgct gatccgccac agcgtccggc ggcggctgta
     8461 ccgggcggct ttccgcagtc gggcagcgtc gctccgcgac gtgcttccta cttccgagag
     8521 gctgtcgcct ctcgggctct ccccatccac cccgtccgga gaaaccgcag gtcggagggg
     8581 tgcgggaaac tctgttgttt ctttcccaag gtgttcgctt ttgcctcggg cggcatctcg
     8641 cgtcacacgc gcgatcgccc gcttcgctgc catccggcag cggtctgagc agtagatacg
     8701 cggccgtttg cccggtgtgt gggcaattgc ggtcccgcag tggcagcggg gcccggcggg
     8761 ccgatctggc aatgcctcgg catcgctccg tactctgggc acgagcaacg ttcctgtctc
     8821 gcccggctaa ggggcgcgag tctgggagcg gacgggtcgg aggtgcgaag tccggcccgt
     8881 tgctctttgg tctggtggga atcctggcac caatcgggcc agaggttccc tccgccactc
     8941 ccgacgcccc ttggggctgg tgtgacttgg agggccgaag agagccccgc cggtgatccg
     9001 gcggggcttt gacgtgcggt cagtgcgtgt gtcggcgaac gatggccaca aagccctgga
     9061 agccgagcgg tccggcaaag tcggcccagt ccaaccgggc tcagcgc
//
