LOCUS       40924_26346        5893 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gene silencing vector pJRTCLCrVA.009, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Tuttle JR, Idris AM, Brown JK, Haigler CH, Robertson D.
  TITLE     Geminivirus-mediated gene silencing from Cotton leaf crumple virus 
            is enhanced by low temperature in cotton
  JOURNAL   Plant Physiol. 148 (1), 41-50 (2008)
  PUBMED    18621976
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Tuttle JR III, Robertson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2008) Plant Biology, North Carolina State 
            University, Box 7612 Gardner Hall, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 5893)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5893)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2008"; volume: "148"; issue: "1"; pages: "41-50"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-FEB-2008) Plant Biology, North Carolina State University, Box 
            7612 Gardner Hall, Raleigh, NC 27695, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2625..2672
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             complement(3234..3632)
                     /codon_start=1
                     /product="AL3"
                     /label=AL3
                     /note="replication enhancer; Ren"
                     /protein_id="ACB32244.1"
                     /translation="MDSRTGEIITARQAENGVYIWEIINPLYFKIYGVEDLLYTRTRIY
                     HVQIRFNHNLRRALHLHKAYLNFQVWTTSLTASGSTYLIRFKQLVMLYLDELGVISLNN
                     VIRAVRFATDKSYVSYVLENHSIKFKFY"
     CDS             complement(3379..3798)
                     /codon_start=1
                     /product="AL2"
                     /label=AL2
                     /note="transactivator of AR1 and BR1; Trap"
                     /protein_id="ACB32243.1"
                     /translation="MRRSNIGLSRMLSSSLSTPPSIKKAHRQAKRRAIRRRRIDLNCGC
                     TIYFHIGCTGHGFTHRGNHHCTSGREWRVYLGDNKSPIFQDIRSRGSAIHQDAYIPRPD
                     PIQPQLTESVASPQSLPELPSLDDISDSFWVDLFN"
     CDS             complement(3695..4759)
                     /codon_start=1
                     /product="AL1"
                     /label=AL1
                     /note="replication initiator; Rep"
                     /protein_id="ACB32242.1"
                     /translation="MPRNPDSFRLQARHIFLTYPKCDIPKNEALQMLQTLSWSVVKPTY
                     IRVAREEHSDGFPHLHCLIQLSGKSNIKNKRFFDLTHPRRSTNFHPNIQAAKDANAVKN
                     YITKDGDCCESGQFKVPGGTKKNKDDVYYNAINAPSLAEALAIIRAGDPRAFIVSYHNI
                     TANLERLFKKAPEQWVPPFPLSSFTNVPEQMQEWADDYFGMGPAARPVRPVSLIVEGDS
                     RTGKTMWARALGPHNYLSGHLDFNNRVYSNNVAYNVIDDVAPQYLKLKHWKELLGAQKD
                     WQSNCKYGKPVQIKGGIPSIVLCNPGEGSSYKDFLDKAENASLKHWTLKNVIFITLDAP
                     LYQEGTQASQEEGD"
     CDS             complement(4285..4680)
                     /codon_start=1
                     /product="AL4"
                     /label=AL4
                     /protein_id="ACB32245.1"
                     /translation="MKLFKCFKPYHGQSSNQHIFELPERSTRTDSHTFTVSSNSLESPT
                     SRIRDFSTLLTPEGRPIFTQTYKQPKTPTPSRITSPKTAIVVNPDSSKCLGVQRRIKTT
                     SIITPSMHLVWRKLLQLSGPEIQGRSS"
     promoter        complement(5250..5268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5278..5294)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5436..5891
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac ttgggatagg
     2221 ttaagaaaat atgtctggct tgtaaacgaa aagaatcggg gtttctaggc attttttaaa
     2281 tagtgaccca ggactcaaaa actagctctc aacttctctc atatgttgtg gagtctggag
     2341 tcctttatat atgtttacgc tatagaaggc atttggggac tctcccgggc ccaccatagg
     2401 actccatacc gcggccatct atataatatt actgatggcc gcgcggattt tggagccccc
     2461 ttttgtcgtt agcgcgacct ttaattcaaa ttaaaggttg cttttttcta tcgtccgatc
     2521 attttgcgcc tgactagcct agatattagc aacaacttcg cggctaagtt gttgaccgtc
     2581 aagtataaat taatgggctt atggcctaat gggtatagag caaaatggca tgcctgcaga
     2641 ctagtgaatt cgctagcgtt aactggccaa tcaaaccgtg ctctttcctc cacaatcttc
     2701 actctaagct cagcgtccct cactgtcccg acttgagcat gcattccgaa tcgatcaaga
     2761 agctgtggtc taagctctcc ttcttctgga ttacctgagc cgatgagaat aaaccgagca
     2821 ggatgtgaga tcgaaatacc ttccctctca acagtgttcc atcctgatgc agcggaatcc
     2881 aaaagaacat ccaccaaatg gtcatctaaa aggttaactt catcgacgta aagaatccct
     2941 cgattagctt tagctagaag tccaggctca aatgccttga caccctcagt gagggctttc
     3001 tcaatgtcaa tggttccaca gaccctatct tcagtagcac ctaatggcaa atccaccatg
     3061 ttgattttag tcattgtaat agtcagttcc tctccattta caaccttttc tctaacttct
     3121 atgcccattg attccggatc ctccgggtcc gagttataag ggtcaccgaa tggccataat
     3181 cctgtgtatg caacgttgaa aattcggatc tatttttacg attcgatcat gaattaataa
     3241 aatttaaatt ttattgaatg attttctagt acataactga catacgactt atcagttgcg
     3301 aaacgaacag ctctaattac attgttaagt gaaattacgc ctaattcgtc taagtacaac
     3361 ataaccaact gtttaaacct aattaaataa gtcgacccag aagctgtcag agatgtcgtc
     3421 cagacttgga agttcaggta agctttgtgg agatgcaacg ctctccgtaa gttgtggttg
     3481 aatcggatct ggacgtggta tatacgcgtc ctggtgtata gcagatcctc tactccgtat
     3541 atcttgaaat ataggggatt tattatctcc cagatataca cgccattctc tgcctgacgt
     3601 gcagtgatga tttcccctgt gcgtgaatcc atgaccggtg cagcctatgt ggaagtatat
     3661 ggtgcaccca cagtttagat caatgcgtcg tcgtctaatc gccctcctct tggcttgcct
     3721 gtgtgccttc ttgatagagg ggggcgtcga gagtgatgaa gataacattc ttgagagtcc
     3781 aatgtttgag cgacgcattt tccgctttat ccaggaaatc tttatagctg gatccctcgc
     3841 ctggattgca aagcacgatt gaagggatcc ctcctttaat ttgaactggt ttcccgtact
     3901 tgcaatttga ctgccagtct ttttgggccc caagaagctc tttccaatgc tttagcttta
     3961 ggtattgcgg tgctacgtca tcaatgacgt tataggcaac attattcgag tacactcgat
     4021 tattgaagtc caggtgtcca ctgaggtaat tatgtgggcc taaagcacga gcccacatcg
     4081 tcttgcccgt tctagaatca ccttccacta tgagactaac tggtcttaca ggccgcgcag
     4141 cgggacccat tccaaaataa tcatccgccc attcttgcat ctgttcagga acgttagtga
     4201 aagaggaaag tggaaatgga gggacccatt gttcaggcgc ctttttgaaa aggcgttcca
     4261 agttcgctgt aatgttatgg taactcacga tgaacgccct tggatctccg gcccggataa
     4321 ttgcaagagc ttccgccaaa ctaggtgcat tgatggcgtt ataatagacg tcgtctttat
     4381 tcttctttgt acccccaggc actttgaact gtccggattc acaacaatcg ccgtctttgg
     4441 tgatgtaatt cttgacggcg ttggcgtctt tggctgcttg tatgtttggg tgaaaattgg
     4501 tcgaccttct ggggtgagta aggtcgaaaa atctcttatt cttgatgttg gactttccag
     4561 agagttggat gagacagtga aggtgtggga atccgtccga gtgctcctct ctggcaactc
     4621 gaatatatgt tggtttgacg actgaccatg ataaggtttg aagcatttga agagcttcat
     4681 tttttgggat atcgcacttg ggataggtta agaaaatatg tctggcttgt aaacgaaaag
     4741 aatcggggtt tctaggcatt ttttaaatag tgacccagga ctcaaaaact agctctcaac
     4801 ttctctcata tgttgtggag tctggagtcc tttatatatg tttacgctat agaaggcatt
     4861 tggggactct cccgggccca ccataggact ccataccgcg gccatctata taatattact
     4921 gatggccgcg cggattttgg agcccccttt tgtcgttagc gcgaccttta attcaaatta
     4981 aaggttgctt ttttctatcg tccaatcatt ttgcgcctga ctagcctaga tattagcaac
     5041 aacttcgcga ctaagttgtt gaccgtcaag tataaattaa tgggcttatg gcccaatggg
     5101 tatagagcaa aatgcccaag cgtgatgccc cgtggcgctt attagcgggt acgtctaagg
     5161 taagtcgaaa tgccaactac tctcccttga gagtattggg ccctaaagtt aataagggac
     5221 ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     5281 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5341 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5401 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     5461 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5521 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5581 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5641 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5701 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5761 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     5821 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5881 gcttacaatt tag
//
