LOCUS       40924_26341        5386 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gene silencing vector pJRTCLCrVA.008, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Tuttle JR, Idris AM, Brown JK, Haigler CH, Robertson D.
  TITLE     Geminivirus-mediated gene silencing from Cotton leaf crumple virus 
            is enhanced by low temperature in cotton
  JOURNAL   Plant Physiol. 148 (1), 41-50 (2008)
  PUBMED    18621976
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Tuttle JR III, Robertson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2008) Plant Biology, North Carolina State 
            University, Box 7612 Gardner Hall, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 5386)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5386)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2008"; volume: "148"; issue: "1"; pages: "41-50"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-FEB-2008) Plant Biology, North Carolina State University, Box 
            7612 Gardner Hall, Raleigh, NC 27695, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2625..2672
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             complement(2727..3125)
                     /codon_start=1
                     /product="AL3"
                     /label=AL3
                     /note="replication enhancer; Ren"
                     /protein_id="ACB32239.1"
                     /translation="MDSRTGEIITARQAENGVYIWEIINPLYFKIYGVEDLLYTRTRIY
                     HVQIRFNHNLRRALHLHKAYLNFQVWTTSLTASGSTYLIRFKQLVMLYLDELGVISLNN
                     VIRAVRFATDKSYVSYVLENHSIKFKFY"
     CDS             complement(2872..3291)
                     /codon_start=1
                     /product="AL2"
                     /label=AL2
                     /note="transactivator of coat protein and BR1; Trap"
                     /protein_id="ACB32238.1"
                     /translation="MRRSNIGLSRMLSSSLSTPPSIKKAHRQAKRRAIRRRRIDLNCGC
                     TIYFHIGCTGHGFTHRGNHHCTSGREWRVYLGDNKSPIFQDIRSRGSAIHQDAYIPRPD
                     PIQPQLTESVASPQSLPELPSLDDISDSFWVDLFN"
     CDS             complement(3188..4252)
                     /codon_start=1
                     /product="AL1"
                     /label=AL1
                     /note="replication initiator; Rep"
                     /protein_id="ACB32237.1"
                     /translation="MPRNPDSFRLQARHIFLTYPKCDIPKNEALQMLQTLSWSVVKPTY
                     IRVAREEHSDGFPHLHCLIQLSGKSNIKNKRFFDLTHPRRSTNFHPNIQAAKDANAVKN
                     YITKDGDCCESGQFKVPGGTKKNKDDVYYNAINAPSLAEALAIIRAGDPRAFIVSYHNI
                     TANLERLFKKAPEQWVPPFPLSSFTNVPEQMQEWADDYFGMGPAARPVRPVSLIVEGDS
                     RTGKTMWARALGPHNYLSGHLDFNNRVYSNNVAYNVIDDVAPQYLKLKHWKELLGAQKD
                     WQSNCKYGKPVQIKGGIPSIVLCNPGEGSSYKDFLDKAENASLKHWTLKNVIFITLDAP
                     LYQEGTQASQEEGD"
     CDS             complement(3778..4173)
                     /codon_start=1
                     /product="AL4"
                     /label=AL4
                     /protein_id="ACB32240.1"
                     /translation="MKLFKCFKPYHGQSSNQHIFELPERSTRTDSHTFTVSSNSLESPT
                     SRIRDFSTLLTPEGRPIFTQTYKQPKTPTPSRITSPKTAIVVNPDSSKCLGVQRRIKTT
                     SIITPSMHLVWRKLLQLSGPEIQGRSS"
     promoter        complement(4743..4761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4771..4787)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4929..5384
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac ttgggatagg
     2221 ttaagaaaat atgtctggct tgtaaacgaa aagaatcggg gtttctaggc attttttaaa
     2281 tagtgaccca ggactcaaaa actagctctc aacttctctc atatgttgtg gagtctggag
     2341 tcctttatat atgtttacgc tatagaaggc atttggggac tctcccgggc ccaccatagg
     2401 actccatacc gcggccatct atataatatt actgatggcc gcgcggattt tggagccccc
     2461 ttttgtcgtt agcgcgacct ttaattcaaa ttaaaggttg cttttttcta tcgtccaatc
     2521 attttgcgcc tgactagcct agatattagc aacaacttcg cgactaagtt gttgaccgtc
     2581 aagtataaat taatgggctt atggcctaat gggtatagag caaaatggca tgcctgcaga
     2641 ctagtgaatt cgctagcgtt aactggccat aatcctgtgt atgcaacgtt gaaaattcgg
     2701 atctattttt acgattcgat catgaattaa taaaatttaa attttattga atgattttct
     2761 agtacataac tgacatacga cttatcagtt gcgaaacgaa cagctctaat tacattgtta
     2821 agtgaaatta cgcctaattc gtctaagtac aacataacca actgtttaaa cctaattaaa
     2881 taagtcgacc cagaagctgt cagagatgtc gtccagactt ggaagttcag gtaagctttg
     2941 tggagatgca acgctctccg taagttgtgg ttgaatcgga tctggacgtg gtatatacgc
     3001 gtcctggtgt atagcagatc ctctactccg tatatcttga aatatagggg atttattatc
     3061 tcccagatat acacgccatt ctctgcctga cgtgcagtga tgatttcccc tgtgcgtgaa
     3121 tccatgaccg gtgcagccta tgtggaagta tatggtgcac ccacagttta gatcaatgcg
     3181 tcgtcgtcta atcgccctcc tcttggcttg cctgtgtgcc ttcttgatag aggggggcgt
     3241 cgagagtgat gaagataaca ttcttgagag tccaatgttt gagcgacgca ttttccgctt
     3301 tatccaggaa atctttatag ctggatccct cgcctggatt gcaaagcacg attgaaggga
     3361 tccctccttt aatttgaact ggtttcccgt acttgcaatt tgactgccag tctttttggg
     3421 ccccaagaag ctctttccaa tgctttagct ttaggtattg cggtgctacg tcatcaatga
     3481 cgttataggc aacattattc gagtacactc gattattgaa gtccaggtgt ccactgaggt
     3541 aattatgtgg gcctaaagca cgagcccaca tcgtcttgcc cgttctagaa tcaccttcca
     3601 ctatgagact aactggtctt acaggccgcg cagcgggacc cattccaaaa taatcatccg
     3661 cccattcttg catctgttca ggaacgttag tgaaagagga aagtggaaat ggagggaccc
     3721 attgttcagg cgcctttttg aaaaggcgtt ccaagttcgc tgtaatgtta tggtaactca
     3781 cgatgaacgc ccttggatct ccggcccgga taattgcaag agcttccgcc aaactaggtg
     3841 cattgatggc gttataatag acgtcgtctt tattcttctt tgtaccccca ggcactttga
     3901 actgtccgga ttcacaacaa tcgccgtctt tggtgatgta attcttgacg gcgttggcgt
     3961 ctttggctgc ttgtatgttt gggtgaaaat tggtcgacct tctggggtga gtaaggtcga
     4021 aaaatctctt attcttgatg ttggactttc cagagagttg gatgagacag tgaaggtgtg
     4081 ggaatccgtc cgagtgctcc tctctggcaa ctcgaatata tgttggtttg acgactgacc
     4141 atgataaggt ttgaagcatt tgaagagctt cattttttgg gatatcgcac ttgggatagg
     4201 ttaagaaaat atgtctggct tgtaaacgaa aagaatcggg gtttctaggc attttttaaa
     4261 tagtgaccca ggactcaaaa actagctctc aacttctctc atatgttgtg gagtctggag
     4321 tcctttatat atgtttacgc tatagaaggc atttggggac tctcccgggc ccaccatagg
     4381 actccatacc gcggccatct atataatatt actgatggcc gcgcggattt tggagccccc
     4441 ttttgtcgtt agcgcgacct ttaattcaaa ttaaaggttg cttttttcta tcgtccgatc
     4501 attttgcgcc tgactagcct agatattagc aacaacttcg cggctaagtt gttgaccgtc
     4561 aagtataaat taatgggctt atggcccaat gggtatagag caaaatgccc aagcgtgatg
     4621 ccccgtggcg cttattagcg ggtacgtcta aggtaagtcg aaatgccaac tactctccct
     4681 tgagagtatt gggccctaaa gttaataagg gacctcgagg gggggcccgg tacccaattc
     4741 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     4801 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     4861 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4921 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4981 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5041 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5101 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5161 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5221 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5281 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5341 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttag
//
