LOCUS       40924_26336       10316 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pJRD215, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Davison J, Heusterspreute M, Chevalier N, Ha-Thi V, Brunel F.
  TITLE     Vectors with restriction site banks. V. pJRD215, a wide-host-range 
            cosmid vector with multiple cloning sites
  JOURNAL   Gene 51 (2-3), 275-280 (1987)
  PUBMED    3036654
REFERENCE   2  (bases 1 to 10316)
  AUTHORS   Piatkov K.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2012) Biology, California Institute of Technology,
            1200 E. California Blvd., 147-75, Pasadena, CA 91125, USA
REFERENCE   3  (bases 1 to 10316)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10316)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 51 
            (2-3), 275-280 (1987)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2012) Biology, California Institute of Technology, 1200 E. 
            California Blvd., 147-75, Pasadena, CA 91125, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..41
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             253..1056
                     /codon_start=1
                     /product="Sm resistance protein A"
                     /label=Sm resistance protein A
                     /protein_id="AFQ55241.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     CDS             1056..1892
                     /codon_start=1
                     /product="Sm resistance protein B"
                     /label=Sm resistance protein B
                     /protein_id="AFQ55242.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     rep_origin      complement(2536..2930)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(2957..3241)
                     /codon_start=1
                     /product="mobilization protein C"
                     /label=mobilization protein C
                     /protein_id="AFQ55243.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     oriT            3272..3359
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             4188..4601
                     /codon_start=1
                     /product="mobilization protein B"
                     /label=mobilization protein B
                     /protein_id="AFQ55245.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     CDS             4598..5566
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             5630..5842
                     /codon_start=1
                     /product="unknown"
                     /label=unknown
                     /note="E"
                     /protein_id="AFQ55247.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             5844..6050
                     /codon_start=1
                     /product="repressor protein F"
                     /label=repressor protein F
                     /protein_id="AFQ55248.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             6080..6916
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             6906..7754
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8282..9073)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    9457..9856
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
ORIGIN
        1 gaattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
       61 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
      121 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
      181 cttgcgggat aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg
      241 aaggaacctc cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg
      301 cctgtcagag gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc
      361 gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt
      421 tggctcaaag gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag
      481 ggtgcatgct tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg
      541 gatttgctca aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg
      601 gttgatcaat gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg
      661 gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgctg
      721 cacgatcttt tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc
      781 gatatggttg tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact
      841 cttcaatgca cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat
      901 ttggcactca tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc
      961 gccttcgctg tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc
     1021 ttctatctgc gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg
     1081 ctcattggca cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga
     1141 aagtttcatt gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca
     1201 ttgctgatga actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc
     1261 ggttgctcgg tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct
     1321 ctcacatcgt tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg
     1381 cgaagctgta tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc
     1441 gctttgcagc tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact
     1501 acgtccacgc ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc
     1561 tacatggcga tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag
     1621 atcccgtcgg tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg
     1681 ctgacagaga cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct
     1741 ctcgtgcgct ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc
     1801 tttccgcagc ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg
     1861 cgatcaagca ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg
     1921 gaacacatca atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc
     1981 cttcccgcaa acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg
     2041 acgcatgggt tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg
     2101 ccgcgcccca cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc
     2161 ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag
     2221 aaattcttca aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag
     2281 cgcagcgaat gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg
     2341 gtggctctgg taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg
     2401 catgttccgc gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc
     2461 ttttaccctc agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc
     2521 ccgtactaac tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac
     2581 gaacccctgc aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg
     2641 cggggtgttg gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc
     2701 agcgcccttg tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct
     2761 ggtctattcg ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg
     2821 ggcaaagccc gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc
     2881 gctttagcgg cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc
     2941 tgtctcggtc gatcattcag cccggctcat ccttctggcg tggcggcaga ccgaacaagg
     3001 cgcggtcgtg gtcgcgttca aggtacgcat ccattgccgc catgagccga tcctccggcc
     3061 actcgctgct gttcaccttg gccaaaatca tggcccccac cagcaccttg cgccttgttt
     3121 cgttcttgcg ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg gcattgattc
     3181 gcgctcgttg ttcttcgagc ttggccagcc gatccgccgc cttgttgctc cccttaacca
     3241 tcttgacacc ccattgttaa tgtgctgtct cgtaggctat catggaggca cagcggcggc
     3301 aatcccgacc ctactttgta ggggagggcg cacttaccgg tttctcttcg agaaactggc
     3361 ctaacggcca cccttcgggc ggtgcgctct ccgagggcca ttgcatggag ccgaaaagca
     3421 aaagcaacag cgaggcagca tggcgattta tcaccttacg gcgaaaaccg gcagcaggtc
     3481 gggcggccaa tcggccaggg ccaaggccga ctacatccag cgcgaaggca agtatgcccg
     3541 cgacatggat gaagtcttgc acgccgaatc cgggcacatg ccggagttcg tcgagcggcc
     3601 cgccgactac tgggatgctg ccgacctgta tgaacgcgcc aatgggcggc tgttcaagga
     3661 ggtcgaattt gccctgccgg tcgagctgac cctcgaccag cagaaggcgc tggcgtccga
     3721 gttcgcccag cacctgaccg gtgccgagcg cctgccgtat acgctggcca tccatgccgg
     3781 tggcggcgag aacccgcact gccacctgat gatctccgag cggatcaatg acggcatcga
     3841 gcggcccgcc gctcagtggt tcaagcggta caacggcaag accccggaga agggcggggc
     3901 acagaagacc gaagcgctca agcccaaggc atggcttgag cagacccgcg aggcatgggc
     3961 cgaccatgcc aaccgggcat tagagcgggc tggccacgac gcccgcattg accacagaac
     4021 acttgaggcg cagggcatcg agcgcctgcc cggtgttcac ctggggccga acgtggtgga
     4081 gatggaaggc cggggcatcc gcaccgaccg ggcagacgtg gccctgaaca tcgacaccgc
     4141 caacgcccag atcatcgact tacaggaata ccgggaggca atagaccatg aacgcaatcg
     4201 acagagtgaa gaaatccaga ggcatcaacg agttagcgga gcagatcgaa ccgctggccc
     4261 agagcatggc gacactggcc gacgaagccc ggcaggtcat gagccagacc cagcaggcca
     4321 gcgaggcgca ggcggcggag tggctgaaag cccagcgcca gacaggggcg gcatgggtgg
     4381 agctggccaa agagttgcgg gaggtagccg ccgaggtgag cagcgccgcg cagagcgccc
     4441 ggagcgcgtc gcgggggtgg cactggaagc tatggctaac cgtgatgctg gcttccatga
     4501 tgcctacggt ggtgctgctg atcgcatcgt tgctcttgct cgacctgacg ccactgacaa
     4561 ccgaggacgg ctcgatctgg ctgcgcttgg tggcccgatg aagaacgaca ggactttgca
     4621 ggccataggc cgacagctca aggccatggg ctgtgagcgc ttcgatatcg gcgtcaggga
     4681 cgccaccacc ggccagatga tgaaccggga atggtcagcc gccgaagtgc tccagaacac
     4741 gccatggctc aagcggatga atgcccaggg caatgacgtg tatatcaggc ccgccgagca
     4801 ggagcggcat ggtctggtgc tggtggacga cctcagcgag tttgacctgg atgacatgaa
     4861 agccgagggc cgggagcctg ccctggtagt ggaaaccagc ccgaagaact atcaggcatg
     4921 ggtcaaggtg gccgacgccg caggcggtga acttcggggg cagattgccc ggacgctggc
     4981 cagcgagtac gacgccgacc cggccagcgc cgacagccgc cactatggcc gcttggcggg
     5041 cttcaccaac cgcaaggaca agcacaccac ccgcgccggt tatcagccgt gggtgctgct
     5101 gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg ctggtgcagc aggctggcca
     5161 gcagatcgag caggcccagc ggcagcagga gaaggcccgc aggctggcca gcctcgaact
     5221 gcccgagcgg cagcttagcc gccaccggcg cacggcgctg gacgagtacc gcagcgagat
     5281 ggccgggctg gtcaagcgct tcggtgatga cctcagcaag tgcgacttta tcgccgcgca
     5341 gaagctggcc agccggggcc gcagtgccga ggaaatcggc aaggccatgg ccgaggccag
     5401 cccagcgctg gcagagcgca agcccggcca cgaagcggat tacatcgagc gcaccgtcag
     5461 caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc gagctggcac gggcaccggc
     5521 accccgccag cgaggcatgg acaggggcgg gccagatttc agcatgtagt gcttgcgttg
     5581 gtactcacgc ctgttatact atgagtactc acgcacagaa gggggtttta tggaatacga
     5641 aaaaagcgct tcagggtcgg tctacctgat caaaagtgac aagggctatt ggttgcccgg
     5701 tggctttggt tatacgtcaa acaaggccga ggctggccgc ttttcagtcg ctgatatggc
     5761 cagccttaac cttgacggct gcaccttgtc cttgttccgc gaagacaagc ctttcggccc
     5821 cggcaagttt ctcggtgact gatatgaaag accaaaagga caagcagacc ggcgacctgc
     5881 tggccagccc tgacgctgta cgccaagcgc gatatgccga gcgcatgaag gccaaaggga
     5941 tgcgtcagcg caagttctgg ctgaccgacg acgaatacga ggcgctgcgc gagtgcctgg
     6001 aagaactcag agcggcgcag ggcgggggta gtgaccccgc cagcgcctaa ccaccaactg
     6061 cctgcaaagg aggcaatcaa tggctaccca taagcctatc aatattctgg aggcgttcgc
     6121 agcagcgccg ccaccgctgg actacgtttt gcccaacatg gtggccggta cggtcggggc
     6181 gctggtgtcg cccggtggtg ccggtaaatc catgctggcc ctgcaactgg ccgcacagat
     6241 tgcaggcggg ccggatctgc tggaggtggg cgaactgccc accggcccgg tgatctacct
     6301 gcccgccgaa gacccgccca ccgccattca tcaccgcctg cacgcccttg gggcgcacct
     6361 cagcgccgag gaacggcaag ccgtggctga cggcctgctg atccagccgc tgatcggcag
     6421 cctgcccaac atcatggccc cggagtggtt cgacggcctc aagcgcgccg ccgagggccg
     6481 ccgcctgatg gtgctggaca cgctgcgccg gttccacatc gaggaagaaa acgccagcgg
     6541 ccccatggcc caggtcatcg gtcgcatgga ggccatcgcc gccgataccg ggtgctctat
     6601 cgtgttcctg caccatgcca gcaagggcgc ggccatgatg ggcgcaggcg accagcagca
     6661 ggccagccgg ggcagctcgg tactggtcga taacatccgc tggcagtcct acctgtcgag
     6721 catgaccagc gccgaggccg aggaatgggg tgtggacgac gaccagcgcc ggttcttcgt
     6781 ccgcttcggt gtgagcaagg ccaactatgg cgcaccgttc gctgatcggt ggttcaggcg
     6841 gcatgacggc ggggtgctca agcccgccgt gctggagagg cagcgcaaga gcaagggggt
     6901 gccccgtggt gaagcctaag aacaagcaca gcctcagcca cgtccggcac gacccggcgc
     6961 actgtctggc ccccggcctg ttccgtgccc tcaagcgggg cgagcgcaag cgcagcaagc
     7021 tggacgtgac gtatgactac ggcgacggca agcggatcga gttcagcggc ccggagccgc
     7081 tgggcgctga tgatctgcgc atcctgcaag ggctggtggc catggctggg cctaatggcc
     7141 tagtgcttgg cccggaaccc aagaccgaag gcggacggca gctccggctg ttcctggaac
     7201 ccaagtggga ggccgtcacc gctgaatgcc atgtggtcaa aggtagctat cgggcgctgg
     7261 caaaggaaat cggggcagag gtcgatagtg gtggggcgct caagcacata caggactgca
     7321 tcgagcgcct ttggaaggta tccatcatcg cccagaatgg ccgcaagcgg caggggtttc
     7381 ggctgctgtc ggagtacgcc agcgacgagg cggacgggcg cctgtacgtg gccctgaacc
     7441 ccttgatcgc gcaggccgtc atgggtggcg gccagcatgt gcgcatcagc atggacgagg
     7501 tgcgggcgct ggacagcgaa accgcccgcc tgctgcacca gcggctgtgt ggctggatcg
     7561 accccggcaa aaccggcaag gcttccatag ataccttgtg cggctatgtc tggccgtcag
     7621 aggccagtgg ttcgaccatg cgcaagcgcc gccagcgggt gcgcgaggcg ttgccggagc
     7681 tggtcgcgct gggctggacg gtaaccgagt tcgcggcggg caagtacgac atcacccggc
     7741 ccaaggcggc aggctgaccc cccccactct attgtaaaca agacattttt atcttttata
     7801 ttcaatggct tattttcctg ctaattggta ataccatgaa aaataccatg ctcagaaaag
     7861 gcttaacaat attttgaaaa attgcctact gagcgctgcc gcacagctcc ataggccgct
     7921 ttcctggctt tgcttccaga tgtatgctct tctgctcctg cagttcgggg gcatggatgc
     7981 gcggatagcc gctgctggtt tcctggatgc cgacggattt gcactgccgg tagaactccg
     8041 cgaggtcgtc cagcctcagg cagcagctga accaactcgc gaggggatcg agcccggggt
     8101 gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga
     8161 aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg
     8221 gcaggttggg cgtcgcttgg tcggtcattt cgcctcgagg cgaaccccag agtcccgctc
     8281 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     8341 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     8401 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     8461 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     8521 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
     8581 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     8641 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     8701 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     8761 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     8821 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     8881 ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     8941 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     9001 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     9061 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc
     9121 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     9181 taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt
     9241 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt
     9301 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     9361 tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc
     9421 gtgaagctag cttggctgca gggggggggg ggggacttcc attgttcatt ccacggacaa
     9481 aaacagagaa aggaaacgac agaggccaaa aagcctcgct ttcagcacct gtcgtttcct
     9541 ttcttttcag agggtatttt aaataaaaac attaagttat gacgaagaag aacggaaacg
     9601 ccttaaaccg gaaaattttc ataaatagcg aaaacccgcg aggtcgccgc cccgtaacct
     9661 gtcggatcac cggaaaggac ccgtaaagtg ataatgatta tcatctacat atcacaacgt
     9721 gcgtggaggc catcaaacca cgtcaaataa tcaattatga cgcaggtatc gtattaattg
     9781 atctgcatca acttaacgta aaaacaactt cagacaatac aaatcagcga cactgaatac
     9841 ggggcaacct catgtccccc cccccccctg caggtcgacg gatccccggg taccataagt
     9901 agaagcagca acccaagtag ctttaccagc atccggttca accagcatag taagaatctt
     9961 actggacatc ggcagttctt cgaacagtgc gccaactacc agctctttct gcagttcatt
    10021 cagggcaccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
    10081 cctgtctctt gatccatgga ttacgcgtta acccgggccc gcggatgcat atgatcttaa
    10141 ggcctaggtc tagagtcttt gttttgacgc cattagcgta cgtaacaatc ctcgttaaag
    10201 gacaaggacc tgagcggaag tgtatcgtac agtagacgga gtatactagt atagtctata
    10261 gtccgtggaa ttattatatt tatctccgac gatattctca tcagtgaaat ccaggg
//
