LOCUS       V005291                 6771 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005291
VERSION     V005291
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Schmelling N, Axmann IM, Dienst D.
  TITLE     The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis
            sp. PCC 6803 as prokaryotic platforms for biosynthesis of cyclic
            plant triterpenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Schmelling N, Axmann IM, Dienst D.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2017) Department of Chemistry - Microbial
            Chemistry, Angstrom and Science for Life Laboratory, Lagerhyddsvagen
            1, Uppsala 75120 Uppsala, Sweden
REFERENCE   3  (bases 1 to 6771)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6771)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-OCT-2017) Department of Chemistry - Microbial Chemistry,
            Angstrom and Science for Life Laboratory, Lagerhyddsvagen 1, Uppsala
            75120 Uppsala, Sweden"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..323
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    384..405
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     CDS             complement(407..1519)
                     /codon_start=1
                     /gene="merR"
                     /product="MerR family transcriptional regulator"
                     /label="merR"
                     /note="cobalt-dependent transcriptional regulator from
                     Synechocystis sp. PCC 6803; coaR"
                     /protein_id="ATY93172.1"
                     /translation="MKTNHLTIKELTDAVGGGVTPRMVRHYHTLGLLPPVQRSEGNYRL
                     YTQQDVQRLQRVIALKQQGFQLSHIRQLLDSHSEESLDPTLMVQLQQQYQAVIQQITRL
                     RQTASALEGLLGRDQSCQITQAEALAQLKQLDVDVQEGLGKLDQLWTNLDAETTTHPEA
                     FQESLKHLLPDLSAYSEITIHLLHQLVLACGDVSLVNAVRLSQGAIASARDALKAGCPV
                     VTDVPVVAAALDQTRLAHLGCTVKTLIDDPHITGLREAEQAFWHHDHWQQRLQQIPQGC
                     VLAIGYAPSVLLTACKLIEQQHIQPALVIGMPIGFSHAPGAKRRLMTSPIPHITIQGSL
                     GGGLLAAVTLNALVETLIAKPDCHCYLTCL"
     gene            complement(407..1519)
                     /gene="merR"
                     /label="merR"
     regulatory      1520..1600
                     /note="promoter including 5' UTR of coaT from Synechocystis
                     sp. PCC 6803 (Moscow wildtype)"
                     /regulatory_class="promoter"
     CDS             1601..2317
                     /label="Venus"
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     terminator      2329..2400
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2416..2443
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     CDS             complement(2468..3169)
                     /gene="rppA"
                     /label="Response regulator RppA"
                     /note="Response regulator RppA from Synechocystis sp.
                     (strain PCC 6803 / Kazusa). Accession#: Q55933"
     CDS             3288..4004
                     /label="mCerulean"
                     /note="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
     terminator      complement(4019..4062)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      4103..4130
                     /note="phage T7 early transcription terminator; T7Te
                     terminator"
                     /regulatory_class="terminator"
     terminator      4103..4130
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    4137..4157
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     promoter        complement(4603..4633)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(4648..4669)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4960..5548)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5722..6579)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6580..6684)
                     /label="AmpR promoter"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga taggtcaatg catgaattcg cggccgcttc tagagactaa agacaagtga
      421 gatagcagtg gcaatctggc tttgcaatca atgtttccac taaagcgttt agcgttactg
      481 cggctagaag tcctccaccg aggctcccct gaatggtgat atggggaatg ggactggtca
      541 tcagtcgtcg ttttgccccc ggagcatgac taaaaccgat cggcattccg atcacaagag
      601 ccggctgaat atgttgttgc tctatcagct tacaggcagt gagtaaaaca gaaggggcat
      661 agccgatcgc cagcacacat ccttggggaa tctgttgtaa ccgctgttgc caatggtcat
      721 ggtgccaaaa agcttgctcg gcttccctaa gccctgtgat gtgagggtcg tcaatcagcg
      781 ttttaaccgt acatcctaaa tgagctaacc gagtttgatc aagagccgca gccacaaccg
      841 gaacatcggt gacgactgga caccctgctt tcagtgcatc tcgtgccgag gcgatcgctc
      901 cctgactcaa tcgaacggcg tttaccaagc taacatcacc acaggccagc actaattgat
      961 gtagtaagtg aatggtaatt tcagagtaag ccgataaatc cggtagcagg tgtttgaggg
     1021 attcctgaaa ggcttctgga tgagttgttg tctccgcatc taggttcgtc cacaactgat
     1081 cgagttttcc taacccctcc tggacatcca catcaagctg tttcagttgg gccagagctt
     1141 ccgcttgggt aatctggcaa ctctggtcgc gtcccagtaa tccttctaaa gcagatgcgg
     1201 tttggcggag tcgagtaatc tgctgaatca cagcctgata ttgctgttgc aactgcacca
     1261 ttagggtggg atcaaggctc tcttcagaat ggctatccag cagttgccga atatgagaca
     1321 actgaaagcc ctgctgtttg agggcaatga ctcgttggag ccgttgtacg tcctgctgag
     1381 tataaaggcg gtagttgccc tctgagcgtt gaacgggggg aagcaatccc agggtgtggt
     1441 aatggcgcac catgcgaggc gtaacgccac ctcccactgc atctgtgagt tctttaatcg
     1501 ttaagtgatt agtcttcatc cctttagttt actcaaaacc ttgacattga cactaatgtt
     1561 aaggtttagg ctgagaaggt aaaaatccaa gttaaaaagc atggctagca agggcgagga
     1621 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1681 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct
     1741 gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta
     1801 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1861 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1921 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1981 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2041 cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
     2101 gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac
     2161 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     2221 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2281 cgccgggatc actctcggca tggacgagct gtacaagtaa ccaggcatca aataaaacga
     2341 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2401 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagctaga
     2461 gtattcatgg cgatagggtg aaccgatagc cttgaccggg aactgtttta attgggcaag
     2521 gacaattttg ttgagcaagc ttgcgtcgta tcaaacgcat ttgggccgcc accacattac
     2581 tcatgggctc ctcatcaaga tcccacagtt gttgccggat cttgctaccg gaaatgatcc
     2641 gctctgggtt ttgcatcaga tattgaaaaa tttgaaattc tcttacggtt aaagcaattt
     2701 cctgtctttc taggtttagt ggctccgaga tagttaccga taacagatta ttactgggat
     2761 caaggctgaa gttgcccaaa gttaaaattt gcggttggaa ttgtggcgat cgccgttgta
     2821 gtgcccgcag tcttgctaat agctctgcca tcacaaacgg ttttgttaga tagtcatctg
     2881 ccccggcatc tagtccttcg acacggtttt ccggttctcc taacgctgtt aacatcaaca
     2941 ccggcaagga attaccctgg gttctcagtt tttgacagag ttccaaaccc gataatcccg
     3001 gcagtaacca atccacaatg gcaagggtgt attccgtcca ttgattttcc aaataatccc
     3061 aagcttggga gccatccgtc acccaatcca ccacatactt ttcactaact agcactttct
     3121 taatagccat tcccaaatcc gtctcatctt ccaccagcaa aattcgcatc gcctctgcct
     3181 tttttataac ggtctgatct tagcggggga aggagatttt cacctgaatt tcataccccc
     3241 tttggcagac tgggaaaatc ttggacaaat tcccaatttg aggtggtatg ggatccaaag
     3301 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3361 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3421 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3481 tgacctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     3541 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3601 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3661 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3721 acgccatcag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     3781 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3841 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3901 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3961 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaacca ggcatcaaat
     4021 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     4081 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
     4141 agtagcggcc gctgcagtcg acaggtcaat ctttctagaa gatctcctac aatattctca
     4201 gctgccatgg aaaatcgatg ttcttctttt attctctcaa gattttcagg ctgtatatta
     4261 aaacttatat taagaactat gctaaccacc tcatcaggaa ccgttgtagg tggcgtgggt
     4321 tttcttggca atcgactctc atgaaaacta cgagctaaat attcaatatg ttcctcttga
     4381 ccaactttat tctgcatttt ttttgaacga ggtttagagc aagcttcagg aaactgagac
     4441 aggaatttta ttaaaaattt aaattttgaa gaaagttcag ggttaatagc atccattttt
     4501 tgctttgcaa gttcctcagc attcttaaca aaagacgtct cttttgacat gtttaaagtt
     4561 taaacctcct gtgtgaaatt attatccgct cataattcca cacattatac gagccggaag
     4621 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     4681 ctcactgcca attgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4741 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4801 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4861 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4921 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4981 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5041 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5101 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5161 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5221 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5281 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5341 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5401 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5461 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5521 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5581 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5641 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5701 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5761 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5821 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5881 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5941 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6001 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6061 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6121 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6181 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6241 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6301 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6361 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6421 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6481 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6541 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6601 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6661 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     6721 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc c
//
