LOCUS       40924_26326        3126 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pJP5603, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Riedel T, Rohlfs M, Buchholz I, Wagner-Dobler I, Reck M.
  TITLE     Complete sequence of the suicide vector pJP5603
  JOURNAL   Plasmid 69 (1), 104-107 (2013)
  PUBMED    22902299
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Riedel T, Reck M.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Microbial Communication, Helmholtz-Centre 
            for Infection Research, Inhoffenstrasse 7, Braunschweig, Lower 
            Saxony 38124, Germany
REFERENCE   3  (bases 1 to 3126)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3126)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2013"; volume: "69"; issue: "1"; pages: "104-107"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-2012) Microbial Communication, Helmholtz-Centre for 
            Infection Research, Inhoffenstrasse 7, Braunschweig, Lower Saxony 
            38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             204..995
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    1273..1294
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1309..1339
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1347..1363
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1371..1387
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(1400..1456)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1457..1473)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1908..2276)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(2309..2418)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2578..2966
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
       61 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
      121 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag
      181 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
      241 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
      301 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
      361 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
      421 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
      481 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
      541 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
      601 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
      661 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
      721 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
      781 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
      841 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
      901 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
      961 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     1021 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     1081 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     1141 ggatctcatg ctggagttct tcgcccaccc cccaatacgc aaaccgcctc tccccgcgcg
     1201 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     1261 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     1321 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     1381 ctatgaccat gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg
     1441 taccgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     1501 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     1561 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggggctcga
     1621 tcccctcgcg agttggttca gctgctgcct gaggctggac gacctcgcgg agttctaccg
     1681 gcagtgcaaa tccgtcggca tccaggaaac cagcagcggc tatccgcgca tccatgcccc
     1741 cgaactgcag gagtggggag gcacgatggc cgctttggtc ggtcttgcct tgctcgtcgg
     1801 tgatgtactt caccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct
     1861 tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc
     1921 gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc
     1981 agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag
     2041 agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg
     2101 tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc
     2161 gacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg
     2221 cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     2281 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     2341 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2401 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2461 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2521 atgaccccga agcagggtta tgcagcggaa aacggccacg atgcgtccgg cgtagaggat
     2581 ctgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact
     2641 aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa
     2701 cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat
     2761 gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt
     2821 tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac ggttgtggac
     2881 aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc aattttgagt
     2941 gacacaggaa cacttaacgg ctgacatggg aattagcttc acgctgccgc aagcactcag
     3001 ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct
     3061 gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga
     3121 gaaagc
//
