LOCUS       Exported               12078 bp DNA     circular SYN 09-DEC-2025
DEFINITION  Bacillus subtilis dCas9 expression vector; integrates into 
            lacA/ganA.
ACCESSION   .
VERSION     .
KEYWORDS    pJMP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12078)
  AUTHORS   Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins 
            JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, 
            Brown ED, Qi LS, Huang KC, Gross CA
  TITLE     A Comprehensive, CRISPR-based Functional Analysis of Essential Genes
            in Bacteria.
  JOURNAL   Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. 
            Epub 2016 May 26.
  PUBMED    27238023
REFERENCE   2  (bases 1 to 12078)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12078)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.003"; journalName: "Cell"; date: "2016-06-2-
            2"; volume: "165"; issue: "6"; pages: "1493-506"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1085..1276
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1378..1518
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1533..1550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      1681..2282
                     /label=ColE1 replication origin
                     /note="High-copy number plasmid replication origin derived
                     from ColE1, commonly used in molecular cloning vectors for 
                     efficient DNA replication in bacterial hosts; id: 4341693"
     CDS             complement(2463..3323)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        3324..3454
                     /label=AmpR promoter
                     /note="Regulatory region that initiates transcription of
                     the ampicillin resistance gene in bacterial plasmids; id: 
                     3672594"
     gene            complement(3539..4038)
                     /label='lacA
                     /note="'lacA"
     regulatory      4045..4126
                     /label=Lambda phage transcription terminator
                     /note="Transcription termination site from bacteriophage
                     lambda that signals RNA polymerase to stop transcription 
                     and release the RNA transcript.; id: 3977482"
     CDS             complement(4138..5262)
                     /codon_start=1
                     /product="XylR is a repressor of xylose operon regulating 
                     promoter Pxyl."
                     /label=Bacillus megaterium xylR
                     /translation="MNQKLILDEILKNSPVSRATLSEITGLNKSTVSSQVNTLLEKDFI
                     FEIGAGQSRGGRRPVMLVFNKNAGYSIGIDIGVDYLNGILTDLEGNIILEKTSDLSSSS
                     ASEVKEILFALIHGFVTHMPESPYGLVGIGICVPGLVDRHQQIIFMPNLNWNIKDLQFL
                     IESEFNVPVFVENEANAGAYGEKVFGMTKNYENIVYISINIGIGTGLVINNELYKGVQG
                     FSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLASLSKEEKNISRKEIVERANKNDV
                     EMLNALQNFGFYIGIGLTNILNTFDIEAVILRNHIIESHPIVLNTIKNEVSSRVHSHLD
                     NKCELLPSSLGKNAPALGAVSIVIDSFLSVTPIS"
     regulatory      5305..5329
                     /label=xylR ribosome binding site cluster
                     /note="Multiple ribosome binding sites associated with the
                     xylR gene, facilitating translation initiation of xylose 
                     metabolic regulatory proteins; id: 3977299"
     promoter        5330..5440
                     /label=XylR promoter
                     /note="Regulatory region that controls transcription
                     initiation for genes responsive to the XylR transcriptional
                     regulator, commonly involved in xylose metabolism pathways;
                     id: 3977300"
     promoter        5376..5507
                     /label=Xylose-inducible promoter PxylA(+66)
                     /note="A strong, constitutive variant of the
                     xylose-inducible PxylA promoter from Bacillus subtilis, 
                     commonly used for controlled gene expression in 
                     Gram-positive bacteria.; id: 3977524"
     regulatory      5508..5529
                     /label=xylA ribosome binding site cluster
                     /note="A cluster of ribosome binding sites that facilitates
                     translation initiation for the xylA gene, enabling 
                     efficient production of xylose metabolic enzymes.; id: 
                     3977566"
     CDS             5578..9684
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     regulatory      9700..9779
                     /label=BBa_B0010 transcriptional terminator
                     /note="A transcriptional terminator sequence that signals
                     the end of transcription, preventing RNA polymerase from 
                     continuing downstream and ensuring proper gene expression 
                     boundaries.; id: 3687631"
     terminator      9788..9828
                     /label=Transcription Terminator Cluster
                     /note="A collection of transcription termination sequences 
                     including B0014, B0012, and T7t terminators that function 
                     to halt RNA polymerase and terminate transcription in 
                     genetic circuits.; id: 3687636"
     mobile_element  9897..11561
                     /label=pMutin Plasmid Insertion Element
                     /note="A pMutin plasmid-derived genetic element used for 
                     insertional mutagenesis, typically serving as a vector for 
                     gene disruption or modification in bacterial systems.; id: 
                     4011928"
     CDS             10380..11117
                     /codon_start=1
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase"
                     /label=Erm
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     regulatory      11281..11379
                     /label=rrnB T1 transcriptional terminator
                     /note="Rho-independent transcriptional terminator that
                     halts RNA polymerase transcription at the end of the 
                     Escherichia coli rrnB ribosomal RNA operon, forming a 
                     stable stem-loop structure in the RNA transcript.; id: 
                     4325218"
     terminator      11390..11420
                     /label=rrnB T2 transcription terminator
                     /note="Transcription termination signal from the E. coli 
                     ribosomal RNA operon rrnB that promotes RNA polymerase 
                     dissociation from DNA template; id: 4381029"
     terminator      11499..11533
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     gene            complement(11576..12075)
                     /label=lacA'
                     /note="lacA'"
ORIGIN
        1 atcctgatat tgtctgcatt tgcgccggag caaaccaaaa acctggtgag acacgccttg
       61 aattagtaga aaagaacttg aagattttca aaggcatcgt tagtgaagtc atggcgagcg
      121 gatttgacgg cattttctta gtcgcgacgc gaggctggat ggccttcccc attatgattc
      181 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
      241 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
      301 tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
      361 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
      421 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
      481 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
      541 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
      601 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
      661 aggctggcgg ggttgcctta ctggttagca gaatgaacca ccgatacgcg agcgaacgtg
      721 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
      781 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
      841 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
      901 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
      961 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
     1021 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc
     1081 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
     1141 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga
     1201 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga
     1261 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     1321 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     1381 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     1441 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     1501 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1561 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1621 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1741 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1801 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1861 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     1921 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2041 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2101 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     2161 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2221 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2281 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2341 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2401 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2461 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2521 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2581 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2641 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2701 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2761 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2821 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2881 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2941 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3001 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3061 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     3121 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3181 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3241 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3301 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3421 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3481 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga atgatgggct
     3541 aatgtgtgtt tacgacaatt ctcacttcat acttttccat cgtcaggtcg cctgacaata
     3601 tgtctcctgt cattatgtcc ttcacactct gatcaaacgt gaccagctgt ttttcttccg
     3661 tgaaattcat gacaaaaata taatcattgt cctgatcctg cctcgcttgt acggagacgc
     3721 cttttccgtg ccgaaccgga aaaactggag agagagacag gtctgtgatc agaccctcat
     3781 agaaatcacg ctgaaattga tcctccaaac gcgcgccgat aaaatacgcc ttgccctgct
     3841 gatactcatg gcttgtgacc gctggcgtgc gcgcataaaa atcttcttga tacaccgctt
     3901 ccactgaagc tgtctttaca tcaatcacgg ttgcataatc cttcatttca tatatttggc
     3961 tgcggtagct gacagcgttt cgatccttcg gatacagggt gtccgtttca agaggctcaa
     4021 ctccaaatat agcttgaaat cgatatctct gcagtcgcga tgattaatta attcagaacg
     4081 ctcggttgcc gccgggcgtt ttttatgcag caatggcaag aacgtcccgg ggagctccta
     4141 acttataggg gtaacactta aaaaagaatc aataacgata gaaaccgctc ctaaagcagg
     4201 tgcatttttt cctaacgaag aaggcaatag ttcacattta ttgtctaaat gagaatggac
     4261 tctagaagaa acttcgtttt taatcgtatt taaaacaatg ggatgagatt caattatatg
     4321 atttctcaag ataacagctt ctatatcaaa tgtattaagg atattggtta atccaattcc
     4381 gatataaaag ccaaagtttt gaagtgcatt taacatttct acatcatttt tatttgcgcg
     4441 ttccacaatc tcttttcgag aaatattctt ttcttcttta gagagcgaag ccagtaacgc
     4501 tttttcagaa gcatataatt cccaacagcc tcgatttcca cagctgcatt tgggtccatt
     4561 aaaatctatc gtcatatgac ccatttcccc agaaaaaccc tgaacacctt tatacaattc
     4621 gttgttaata acaagtccag ttccaattcc gatattaata ctgatgtaaa cgatgttttc
     4681 atagtttttt gtcataccaa atactttttc accgtatgct cctgcattag cttcattttc
     4741 aacaaaaacc ggaacattaa actcactctc aattaaaaac tgcaaatctt tgatattcca
     4801 atttaagtta ggcatgaaaa taatttgctg atgacgatct acaaggcctg gaacacaaat
     4861 tcctattccg actagaccat aaggggactc aggcatatgg gttacaaaac catgaataag
     4921 tgcaaataaa atctctttta cttcactagc ggaagaacta gacaagtcag aagtcttctc
     4981 gagaataata tttccttcta agtcggttag aattccgtta agatagtcga ctcctatatc
     5041 aataccaatc gagtagcctg cattcttatt aaaaacaagc attacaggtc ttctgccgcc
     5101 tctagattgc cctgccccaa tttcaaaaat aaaatctttt tcaagcagtg tatttacttg
     5161 agaggagaca gtagacttgt ttaatcctgt aatctcagag agagttgccc tggagacagg
     5221 ggagttcttc aaaatttcat ctaatattaa tttttgattc atttttttta ctaaagcttg
     5281 atctgcaatt tgaataataa ccactccttt gtttatccac cgaactaagt tggtgttttt
     5341 tgaagcttga attagatatt taaaagtatc atatctaata ttataactaa attttctaaa
     5401 aaaaacattg aaataaacat ttattttgta tatgatgaga taaagttagt ttattggata
     5461 aacaaactaa ctcaattaag atagttgatg gataaacttg ttcacttaaa tcaaaggggg
     5521 aaatgacaaa tggtccaaac tagtgatatc taaaaatcaa agggggaaat gggatccatg
     5581 gataagaaat actcaatagg cttagctatc ggcacaaata gcgtcggatg ggcggtgatc
     5641 actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac
     5701 agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg
     5761 actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat
     5821 ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt
     5881 gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat
     5941 atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa
     6001 ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg
     6061 attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg
     6121 gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt
     6181 aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga
     6241 ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc
     6301 attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat
     6361 gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa
     6421 attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta
     6481 ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg
     6541 attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa
     6601 caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt
     6661 tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa
     6721 aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag
     6781 caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct
     6841 attttgagaa gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa
     6901 aaaatcttga cttttcgaat tccttattat gttggtccat tggcgcgtgg caatagtcgt
     6961 tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt
     7021 gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat
     7081 cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat
     7141 aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca
     7201 ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt
     7261 aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca
     7321 ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt
     7381 aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta
     7441 acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac
     7501 ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt
     7561 ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat
     7621 tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt
     7681 ttgacattta aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat
     7741 gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta
     7801 aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt
     7861 gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg
     7921 aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt
     7981 gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac
     8041 atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt cgatgccatt
     8101 gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat
     8161 aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac
     8221 tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg
     8281 aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg
     8341 gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact
     8401 aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa
     8461 ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac
     8521 catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat
     8581 ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg
     8641 attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat
     8701 atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct
     8761 ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc
     8821 acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag
     8881 acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct
     8941 cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat
     9001 tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa
     9061 gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt
     9121 ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat
     9181 agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa
     9241 aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat
     9301 tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag
     9361 cataagcatt atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt
     9421 ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca
     9481 atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc
     9541 gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa
     9601 gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat
     9661 ttgagtcagc taggaggtga ctaactcgag taaggatctc caggcatcaa ataaaacgaa
     9721 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     9781 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatacc tagggatata
     9841 ttccgcttcc tcgctcactg actcggtctc ggatccgagc tcccgcgggc ggccgcactc
     9901 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9961 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10021 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    10081 acgaggccct ttcgtcttca agaattgatc ctctagcaca aaagaaaaac gaaatgatac
    10141 accaatcagt gcaaaaaaag atataatggg agataagacg gttcgtgttc gtgctgactt
    10201 gcaccatatc ataaaaatcg aaacagcaaa gaatggcgga aacgtaaaag aagttatgga
    10261 aataagactt agaagcaaac ttaagagtgt gttgatagtg cagtatctta aaattttgta
    10321 taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg agtgattaca
    10381 tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa
    10441 taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga acaggtaaag
    10501 ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt gaattagaca
    10561 gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc actttaattc
    10621 accaagatat tctacagttt caattcccta acaaacagag gtataaaatt gttgggagta
    10681 ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc catgcgtctg
    10741 acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt caccgaacac
    10801 tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg ccagcggaat
    10861 gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa acttacccgc cataccacag
    10921 atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg gtcaatcgag
    10981 aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa
    11041 acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt tatctattat
    11101 ttaacgggag gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt acacgttact
    11161 aaagggaatg tagataaatt attaggtata ctactgacag cttccaagga gctaaagagg
    11221 tccctagact ctagacccgg ggatctctgc agtgagatct ggtaatgact ctctagcttg
    11281 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
    11341 ttgtcggtga acgctctcct gagtaggaca aatccgccgc tctagctaag cagaaggcca
    11401 tcctgacgga tggccttttt gcgtttctac aaactcttgt taactctaga gctgcctgcc
    11461 gcgtttcggt gatgaagatc ttcccgatga ttaattaatt cagaacgctc ggttgccgcc
    11521 gggcgttttt tatgcagcaa tggcaagaac gttgctctag agcggccgca tcgattcaca
    11581 gtggcaatct cccccgtatt cgtttgaaat gtgccacatt aacagcgccg ggtgatgtcc
    11641 gtatcgttct gctaataagc ggttgatgtg ccgtgttttt tctcggtaga ctttagatgt
    11701 gaggcagtgg ttgtgccttc cgccgtgcag ctgtttgacg cgggaggcat tgacgcgcaa
    11761 aacttccgga taggtttgcg acagccaggc cggacgggct ccgctcggcg ttgctaatat
    11821 gacccggccg cctatactgt gaatccgctc aaaaatatca tccagccatt caaattgata
    11881 tacgccctcc tccggctcaa gtgcgctcca tgcaaaaatg ccgacagaaa acgtattcgt
    11941 atgagaaagc ttcatcagtt tgatatcgtc agctaaaata tcgggccgat ccagccactg
    12001 atcggggttg tagtctcccc catggagcat aaattttgct tttgttacgt gcgttttttc
    12061 aagctttgac atcacccc
//
