LOCUS       40924_26296        6797 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration expression vector pJM251, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Meisner J, Goldberg JB.
  TITLE     Escherichia coli rhaSR-PrhaBAD inducible promoter system allows 
            tightly controlled gene expression over a wide range in Pseudomonas 
            aeruginosa
  JOURNAL   Appl. Environ. Microbiol. (2016) In press
  PUBMED    27613678
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Meisner J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2016) Biochemistry, Emory University School of 
            Medicine, 1510 Clifton Road NE, Rollins Research Center, room G223, 
            Atlanta, GA 30322, USA
REFERENCE   3  (bases 1 to 6797)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6797)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-AUG-2016) Biochemistry, Emory University School of Medicine, 
            1510 Clifton Road NE, Rollins Research Center, room G223, Atlanta, 
            GA 30322, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        827..845
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(878..1753)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1780..2064
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(2119..2135)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2161..2179)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2353..2400)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     oriT            complement(2607..2715)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(4140..4728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5553..6740)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        complement(join(6788..6797,1..19))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 atcgatgata agctgtcaaa catgagaatt aattcatgtt ctttcctgcg ttatcccctg
       61 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      181 ctctccccgc gcgttggccg attcattaat gcagcgaatt agcttcaaaa gcgctctgaa
      241 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat cccctgattc
      301 cctttgtcaa cagcaatgga ttttcaatgg gtcaacatga aatccggctg ttgccctggg
      361 acggcaggtt tgacggcagg cttccaggct ctctcgcaca cgctgggaac actctgggaa
      421 caaacgcgag tgaaaatgga cagtagggcc caggaataat gcggcctcca gcgaggttgg
      481 ggcgtttcat atgagttcga atctcaccgc ctccgccata taacctgctg ttttcattga
      541 tgtttccatt cgacccactc tcaggagtga acgatttggg aacaaactgg gaacgaggcg
      601 cagaaaaaag gggccccgca gggccccttt ttcgttgctt caggaaagtt tcagcgcctg
      661 attcagcaga cctacgatgt cggggccgtc cttgctgaat tagctttatg cttgtaaacc
      721 gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca attaattagt
      781 aatataatct attaaaggtc attcaaaagg tcatccaccg gcgcgcaatt aaccctcact
      841 aaagggaaca aaagctggag ctcgaatccc caaattatga caacttgacg gctacatcat
      901 tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa
      961 atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag
     1021 gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc
     1081 ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc
     1141 aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt
     1201 actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt
     1261 ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc
     1321 cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg
     1381 cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat
     1441 gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat
     1501 gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa
     1561 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat
     1621 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg
     1681 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt
     1741 gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt
     1801 gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa
     1861 ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta
     1921 acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac
     1981 tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga cgctttttat
     2041 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagactagtg ctctgcagga
     2101 attcctcgag aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc
     2161 cctatagtga gtcgtattac gcgcgccggt ggatgacctt ttgaatgacc tttaatagat
     2221 tatattacta attaattggg gaccctagag gtcccctttt ttattttaaa aattttttca
     2281 caaaacggtt tacaagcata aagctaattc cattgagtaa gtttttaagc acatcagctt
     2341 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc
     2401 aagatcccca attcgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2461 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2521 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2581 gcaggaaaga acatgctaga gcttatcggc cagcctcgca gagcaggatt cccgttgagc
     2641 accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact
     2701 tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt
     2761 tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg
     2821 accccgaagc agggttatgc agcggaaagt ataccttaac ctttttcgtt gcttcaggaa
     2881 agtttcagcg cctgattcag cagacctacg atgtcggggc cgtccttgct gatccacttg
     2941 gcgtaatgct tgaagatcat cgccgtcgag gtgtgcccca tctggtccgc gatccattcg
     3001 ggagtggcga tgccgctgct gagcatctgg ctggcgaagg tgtggcggca ctgattggga
     3061 ccacgcgagc ggacgcccgc attcctgagg tggccatgcc accagccgtt gcgcagcgta
     3121 tccgacgtgg agtacgccgc cccgctagca ctgttgtgga acacgaagcg tacccgttga
     3181 ggctttctgg tccggttgtc gcggtctatc acctcgatct gtacgggcgg aaggcgtctg
     3241 gttagcttgg cctgctgctg gagtgcgcgc agcgccgggg ccagtagctg gaccttcctc
     3301 gtcgatcggc gagtcttggt gaccttgtac tggctgcgta cacgagcgcg gcggaaaacc
     3361 acggtccccg tgtccaaatc gacatcttcc caggccagag ctatggcctc gctgacgcgg
     3421 gggccgctcc agatcatgaa ctccgccagg ttcagctcac cgatgcgcgc ggtttcagta
     3481 ccgaggatga ggtcgatttc ctcacgagtg aatgggtctg ggtcatccgc atccggaaga
     3541 gtgatcacga tgccgtctgt tgggtcgtgc gcgctccggt tccgagttcg atacagccgg
     3601 aagatctgcc gcaggttgct gacgatctct cgcaccgtct tgttgtgcag cttgggcatg
     3661 agcgtgttct gcacccagtc ttgaatatcc aggtggtcga tgctgtcggc ctggctgtca
     3721 ccccagcgag gtcggatgtg ggtttccacg cggctggtgt agccgcggaa gccgctggcg
     3781 gcaatctggt tgcgtttgat gtcgagccag aggtcgatgt aatggcccaa ggtgttgctt
     3841 ttcacccgcg gtgagtccgg gaagtgtcgg ctgtagctga agactccttg cttgatctcg
     3901 tagttgatga tgccggccag ccgttcggca ttggctatat tctcgggcgt tgcgtcccct
     3961 ggtatggact ccctacacag ctcgccctgg tagcggaagt agatcctgat ccgttttccg
     4021 cgtacctcga ctccgtctgc catctggttc ccctgatcca caccaagcgt tcaagctggc
     4081 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4141 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4201 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4261 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4321 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4381 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4441 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4501 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4561 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4621 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4681 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4741 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4801 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4861 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4921 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4981 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5041 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5101 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5161 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5221 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5281 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5341 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5401 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtcaagggtt
     5461 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
     5521 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
     5581 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
     5641 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa
     5701 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
     5761 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     5821 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
     5881 tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     5941 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     6001 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     6061 tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc
     6121 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcggcgctct
     6181 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     6241 gccgccgcaa ggaatggtgc atgtaaggag atggcgccca acagtccccc ggccacgggg
     6301 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     6361 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     6421 ccggccacga tgcgtccggc gtagagaatc cacaggacgg gtgtggtcgc catgatcgcg
     6481 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
     6541 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
     6601 acgccatagt gactggcgat gctgtcggaa tggacaatat cccgcaagag gcccggcagt
     6661 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     6721 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     6781 ctaccgcatt aaagcta
//
