LOCUS       V005302                 4376 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005302
VERSION     V005302
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Stoudenmire JL, Essock-Burns T, Weathers EN, Solaimanpour S, Mrazek
            J, Stabb EV.
  TITLE     An iterative synthetic approach to engineer a high-performing
            PhoB-specific reporter
  JOURNAL   Appl. Environ. Microbiol. (2018) In press
   PUBMED   29752265
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Stoudenmire JL, Stabb EV.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2018) Microbiology, University of Georgia, 120
            Cedar Street, Athens, GA 30605, USA
REFERENCE   3  (bases 1 to 4376)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-JAN-2018) Microbiology, University of Georgia, 120 Cedar Street,
            Athens, GA 30605, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..532
                     /gene="dhfrI"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Escherichia
                     coli. Accession#: P00382"
     CDS             complement(581..1288)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     primer_bind     1390..1406
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1593..2300
                     /label="GFP"
                     /note="Aequorea victoria green fluorescent protein"
     promoter        complement(2380..2398)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2414..2802)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            complement(4075..4184)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
ORIGIN
        1 ccgcttgaca taaagtccaa gatagtctat aatgcggata gcgcgcctct gaggaagaat
       61 tgtgaaacta tcactaatgg tagctatatc gaagaatgga gttatcggga atggccctga
      121 tattccatgg agtgccaaag gtgaacagct cctgtttaaa gctattacct ataaccaatg
      181 gctgttggtt ggacgcaaga cttttgaatc aatgggagca ttacccaacc gaaagtatgc
      241 ggtcgtaaca cgttcaagtt ttacatctga caatgagaac gtattgatct ttccatcaat
      301 taaagatgct ttaaccaacc taaagaaaat aacggatcat gtcattgttt caggtggtgg
      361 ggagatatac aaaagcctga tcgatcaagt agatacacta catatatcta caatagacat
      421 cgagccggaa ggtgatgttt actttcctga aatccccagc aattttaggc cagtttttac
      481 ccaagacttc gcctctaaca taaattatag ttaccaaatc tggcaaaagg gttaagcgcg
      541 caaaaaaggc tgggctgtcc cagccttttt gcggccgtta cttgtacagc tcgtccatgc
      601 cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt
      661 tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct
      721 tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca
      781 gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg
      841 cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc
      901 gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca
      961 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
     1021 gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga
     1081 actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct
     1141 tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt
     1201 ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca
     1261 tgttatcctc ctcgcccttg ctcaccatgt ttaaaacctc cctacgtacg cggggatcct
     1321 attatagaat aacttcgact ttatgtcaat taattaaaaa accgccctgc aaggcggttt
     1381 tttcgttttg taaaacgacg gccagtaaaa aggcgcactg aacaatgcgc cttttttata
     1441 agatcataaa agacccttca tttatgaagg gtctttttct cgagcccggg catgcctgtc
     1501 ataaatctgt catatcactg acatatttct gtcacatgtt catggtattt tttgagaatc
     1561 agtcgacagg agctaaggaa gctaaaatgg ctagcaaagg agaagaactc ttcactggag
     1621 ttgtcccaat tcttgttgaa ttagatggtg atgttaacgg ccacaagttc tctgtcagtg
     1681 gagagggtga aggtgatgca acatacggaa aacttaccct gaagttcatc tgcactactg
     1741 gcaaactgcc tgttccatgg ccaacactag tcactactct gtgctatggt gttcaatgct
     1801 tttcaagata cccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
     1861 gttatgtaca ggaaaggacc atcttcttca aagatgacgg caactacaag acacgtgctg
     1921 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgacttca
     1981 aggaagatgg caacattctg ggacacaaat tggaatacaa ctataactca cacaatgtat
     2041 acatcatggc agacaaacaa aagaatggaa tcaaagtgaa cttcaagacc cgccacaaca
     2101 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     2161 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     2221 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     2281 atggcatgga tgaactgtac aacgtcgcaa acgacgaaaa ctacgcagca tcagtttgag
     2341 gatccataaa aaaggggacc tctagggtcc ccttttttcc ctatagtgag tcgtattagg
     2401 gcccgaattc ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg
     2461 ctctaagggc ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta
     2521 aaagctttaa aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct
     2581 atttaagttg ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa
     2641 catgagagct tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc
     2701 gttaaacatg agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct
     2761 tagtacgtac tatcaacagg ttgaactgct gatcttcaga tccccgggga tcgatatctg
     2821 cagcagcaac ggtgaagatt atagagacca agcctttgaa acgattttca aaaggaacag
     2881 agaaaaatta aaacgactca ccatagcaag ggcagcgaga gcggaattga aaaagttacc
     2941 gaaagaagaa aaaagcacat tcagttggtc agcatcgatc cataaaagaa gataatcgac
     3001 ccaactaacg cgcttcgctt gttcgcacat tagaaaagaa agtgggctta actcgcttat
     3061 gctcgttgcg gcaactttca tttctaatcg tgcagctctt aatattttct ttttaattct
     3121 ttttgaattg aagagcaaaa gaagtcggca tgtatgctga taggtgagtt ttgagctaga
     3181 tttcggtaat actcacccta gaggataacc gctttcgcta agtttatcac tctagtgtga
     3241 accctgattg cttgccgact ggagccgttc atcgccatca ttcgcttgaa ttctaataca
     3301 ctattcaagt gcagagatta cgggctgtat tgcccccatc ctcaccgttc tctgccgacc
     3361 tctgcatcag taccttgcaa gggtttcccc aatctacaag cctctttgcc tagctgctta
     3421 cgctagttct tgcttcagtc cactcatttg gtgtgcgtta tcggatgtta cgctagtctc
     3481 tcgtccagta tctgggcgaa atatggtatg aaaaccaacc gcatgatgct gtcccacaac
     3541 cctagtgact ctaaacccaa ccactgattg cttggaaata cttgcagttc ttatgtgata
     3601 tgatctatca tgaaaggact tacaacgccg tctaaacgta gttaagtatt tcgaaaaaac
     3661 caagcttgca ggcttggttt tttcacatct gggcataagg ttacggctaa atgctcaaga
     3721 tctcaatgat caattttatg atcctctggt tcatagacaa aaaagccact cttttgagtg
     3781 gcttttttgt ttctattctt tgtgtgattt tacatacatt aaatgaatac ctgtcggggt
     3841 tatttgggtt aggttataac tgtattcata accgttttca tcaagcaatt ttatacaata
     3901 tagtgcaaat acaaccgcaa atactacacc taatatcatg ctataaatac acgttctatt
     3961 gatagtacta gaactagact ggaaggcagt acaccttgat aggtgggctg cccttcctgg
     4021 ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc
     4081 agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga
     4141 aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga
     4201 tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg
     4261 atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaagc
     4321 gctgcttccc tgctgttttg tggaatatct accgactgga aacaggcaaa tgcagg
//
