LOCUS       V005303                 4663 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005303
VERSION     V005303
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Stoudenmire JL, Essock-Burns T, Weathers EN, Solaimanpour S, Mrazek
            J, Stabb EV.
  TITLE     An iterative synthetic approach to engineer a high-performing
            PhoB-specific reporter
  JOURNAL   Appl. Environ. Microbiol. (2018) In press
   PUBMED   29752265
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Stoudenmire JL, Stabb EV.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2018) Microbiology, University of Georgia, 120
            Cedar Street, Athens, GA 30605, USA
REFERENCE   3  (bases 1 to 4663)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-JAN-2018) Microbiology, University of Georgia, 120 Cedar Street,
            Athens, GA 30605, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..532
                     /gene="dhfrI"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Escherichia
                     coli. Accession#: P00382"
     CDS             complement(581..1288)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     primer_bind     1390..1406
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1488..2567)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(2568..2645)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    2747..2763
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2780..2796
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(2860..2876)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(2951..2969)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2990..3378)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            complement(4362..4471)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
ORIGIN
        1 ccgcttgaca taaagtccaa gatagtctat aatgcggata gcgcgcctct gaggaagaat
       61 tgtgaaacta tcactaatgg tagctatatc gaagaatgga gttatcggga atggccctga
      121 tattccatgg agtgccaaag gtgaacagct cctgtttaaa gctattacct ataaccaatg
      181 gctgttggtt ggacgcaaga cttttgaatc aatgggagca ttacccaacc gaaagtatgc
      241 ggtcgtaaca cgttcaagtt ttacatctga caatgagaac gtattgatct ttccatcaat
      301 taaagatgct ttaaccaacc taaagaaaat aacggatcat gtcattgttt caggtggtgg
      361 ggagatatac aaaagcctga tcgatcaagt agatacacta catatatcta caatagacat
      421 cgagccggaa ggtgatgttt actttcctga aatccccagc aattttaggc cagtttttac
      481 ccaagacttc gcctctaaca taaattatag ttaccaaatc tggcaaaagg gttaagcgcg
      541 caaaaaaggc tgggctgtcc cagccttttt gcggccgtta cttgtacagc tcgtccatgc
      601 cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt
      661 tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct
      721 tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca
      781 gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg
      841 cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc
      901 gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca
      961 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
     1021 gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga
     1081 actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct
     1141 tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt
     1201 ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca
     1261 tgttatcctc ctcgcccttg ctcaccatcg gccgcttccc ttcctaaagt cttgatccta
     1321 ttatagaata cacttgactt tatgtcaact taattaaaaa accgccctgc aaggcggttt
     1381 tttcgttttg taaaacgacg gccagtaaaa aggcgcactg aacaatgcgc cttttttata
     1441 agatcataaa agacccttca tttatgaagg gtctttttct cgagtcactg cccgctttcc
     1501 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1561 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
     1621 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
     1681 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
     1741 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
     1801 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
     1861 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
     1921 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
     1981 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
     2041 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
     2101 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
     2161 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
     2221 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
     2281 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
     2341 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
     2401 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
     2461 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
     2521 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
     2581 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg
     2641 gtgtcaacgt aaatgcatgc cgcttcgcct tcgcgcgcga attgcaagct gatccgggct
     2701 tatcgactgc acggaagggc gaatttttat caaaaagagt gttgacttgt gagcggataa
     2761 caatgatact tagattcaat tgtgagcgga taacaatttc acacagctag tctcgagccc
     2821 gggcatgcct aggtacctct agagtcgaca ggcctcatgg tcatagctgt ttcctgatat
     2881 caaggagata tacatatggc tagcgagctc ggatccataa aaaaggggac ctctagggtc
     2941 cccttttttc cctatagtga gtcgtattag ggcccaccgg gaattcccat gtcagccgtt
     3001 aagtgttcct gtgtcactca aaattgcttt gagaggctct aagggcttct cagtgcgtta
     3061 catccctggc ttgttgtcca caaccgttaa accttaaaag ctttaaaagc cttatatatt
     3121 cttttttttc ttataaaact taaaacctta gaggctattt aagttgctga tttatattaa
     3181 ttttattgtt caaacatgag agcttagtac gtgaaacatg agagcttagt acgttagcca
     3241 tgagagctta gtacgttagc catgagggtt tagttcgtta aacatgagag cttagtacgt
     3301 taaacatgag agcttagtac gtgaaacatg agagcttagt acgtactatc aacaggttga
     3361 actgctgatc ttcagatccc cggtctgcag attttctttt taattctttt tgaattgaag
     3421 agcaaaagaa gtcggcatgt atgctgatag gtgagttttg agctagattt cggtaatact
     3481 caccctagag gataaccgct ttcgctaagt ttatcactct agtgtgaacc ctgattgctt
     3541 gccgactgga gccgttcatc gccatcattc gcttgaattc taatacacta ttcaagtgca
     3601 gagattacgg gctgtattgc ccccatcctc accgttctct gccgacctct gcatcagtac
     3661 cttgcaaggg tttccccaat ctacaagcct ctttgcctag ctgcttacgc tagttcttgc
     3721 ttcagtccac tcatttggtg tgcgttatcg gatgttacgc tagtctctcg tccagtatct
     3781 gggcgaaata tggtatgaaa accaaccgca tgatgctgtc ccacaaccct agtgactcta
     3841 aacccaacca ctgattgctt ggaaatactt gcagttctta tgtgatatga tctatcatga
     3901 aaggacttac aacgccgtct aaacgtagtt aagtatttcg aaaaaaccaa gcttgcaggc
     3961 ttggtttttt cacatctggg cataaggtta cggctaaatg ctcaagatct caatgatcaa
     4021 ttttatgatc ctctggttca tagacaaaaa agccactctt ttgagtggct tttttgtttc
     4081 tattctttgt gtgattttac atacattaaa tgaatacctg tcggggttat ttgggttagg
     4141 ttataactgt attcataacc gttttcatca agcaatttta tacaatatag tgcaaataca
     4201 accgcaaata ctacacctaa tatcatgcta taaatacacg ttctattgat agtactagaa
     4261 ctagactgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
     4321 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     4381 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     4441 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     4501 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     4561 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgct gcttccctgc
     4621 tgttttgtgg aatatctacc gactggaaac aggcaaatgc agg
//
