LOCUS       V005306                 8525 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005306
VERSION     V005306
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Lo J, Zheng T, Hon S, Olson DG, Lynd LR.
  TITLE     The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is
            necessary for ethanol production in Clostridium thermocellum and
            Thermoanaerobacterium saccharolyticum
  JOURNAL   J. Bacteriol. (2015) In press
   PUBMED   25666131
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Lo J, Zheng T, Hon S, Olson DG, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2015) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 8525)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol. (2015) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-JAN-2015) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(561..1418)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1419..1523)
                     /label="AmpR promoter"
     CDS             3192..3839
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             3860..4405
                     /codon_start=1
                     /gene="hpt"
                     /product="hpt"
                     /EC_number="2.4.2.8"
                     /label="hpt"
                     /note="hypoxanthine phosphoribosyltransferase"
                     /protein_id="AJO16025.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            3860..4405
                     /gene="hpt"
                     /label="hpt"
     terminator      5123..5311
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(5571..6159)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6246..6824)
                     /codon_start=1
                     /gene="tdk"
                     /product="tdk"
                     /EC_number="2.7.1.21"
                     /label="tdk"
                     /note="thymidine kinase"
                     /protein_id="AJO16027.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(6246..6824)
                     /gene="tdk"
                     /label="tdk"
     CDS             complement(7524..8525)
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 ctatttagtt atttgtttgg tcacttataa ctttaacaga tggggttttt ctgtgcaacc
       61 aattttaagg gttttcaata ctttaaaaca catacatacc aacacttcaa cgcacctttc
      121 agcaactaaa ataaaaatga cgttatttct atatgtatca agaatagaaa gaactcgttt
      181 ttcgctacgc tcaaaacgca aaaaaagcac tcattcgagt gctttttctt atcgctccaa
      241 atcatgcgat tttttcctct ttgcttttct ttgctcacga agttctcgat cacgctgcaa
      301 aacatcttga agcgaaaaag tattcttctt ttcttccgat cgctcatgct gacgcacgaa
      361 aagccctcta ggcgcatagg aacaactcct aaatgcatgt gaggggtttt ctcgtccatg
      421 tgaacagtcg catacgcaat attttgtttc ccatagcggc cgcgattatc aaaaaggatc
      481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
      541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
      601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      661 cgcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
      721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      841 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
      901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1141 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     1381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     1441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     1561 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcg atggcttgga
     1621 ggagctcttt tatcctatga gggctttaaa gaatatggat taaatattgt tgcagcgttt
     1681 gatgttgatg aaagtaaaat cggcactgaa atttgcggga gaaaaatttt ccctttggac
     1741 aaaatgaagg aactgtgccg gcggatgaaa attcgtatcg gtataataac cgtccctgca
     1801 gattcagccc aaaaagtatg cgacatgctt gtagacagcg gaatatatgc aatatggaat
     1861 tttgcacctg ttcaccttaa agtaccggat aacattttag tccagcaaga aaacatggcg
     1921 gcatcgctgg ctgtactttc cgcacacttg gcagaagcaa taaagaataa tggaatcgga
     1981 aaaggagata aggaaaatga cgaaaatagc gaataaatac gaagttattg ataatgttga
     2041 aaagcttgaa aaggctttga aacgtttaag agaagctcaa agtgtttatg caacctatac
     2101 acaggagcag gttgaaaagg aatttttgga cagactggac gaaatggtgg aacaggcttt
     2161 tgacgaccag tgcacaggta caaatccaag atacccgctt atgaatgaaa tcaggcaaat
     2221 gtatctgaac gcttattacg gaggtgcgaa gaaatgaagc ttgaaaaggt tatagcggta
     2281 agaacggaca aaaccgttta cagagacgga gacaaggcaa taaaggtctt ttccagcaac
     2341 tattcaaagg ccaacgtatt gaatgaagct ttaaaccagg ccagggtgga agaaaccggg
     2401 cttaacattc cgaagcttct tgaagttaca aaaattgatg gcaaatgggc aattgtgacg
     2461 gagtttattg aaggaaagac cttagagcag cttatgaaag aaaatccctt aaaatatgat
     2521 gaatatatga acctctttgt tgatattcag ctcgaagtgt taagcaaaag ggctccttta
     2581 ttaaacaagc tgaaagacaa aatgcacaga aaaatcgtgg gaataggcat ggaaaagatt
     2641 tttttgcccg aggatgcatt gataaagtat ttcttttccg aaagagagga aaagattcta
     2701 aagagttttg gaaatactga tgaatattgt gtgcagagta caattctatg gacaagaaaa
     2761 gaggctttgt caaaactttt tcgtctggga atgaggatgg attttaaaaa gctggatact
     2821 ttggagggcg aggtggtttt tcaggaaaca aacagggcgc gtctgttttc ttttatatgc
     2881 aataattact gtatctctct ggcattgcca ggttttaata aagattaaaa ttattgacta
     2941 gaaataaaaa aattgtccat aatattaatg gacaaaaaaa caaagaatta catcaaagga
     3001 agataaaaat actttgttaa aaaattaatt attttttatc taaactattg aaaatgaaaa
     3061 taaaataata taaaatgaat catagtgcaa gagatacttg ccagaggatg aatattttac
     3121 tgcattcatg ctttatggca gctaatagag gcattaaatt aaattttaat ttacaatagg
     3181 aggcgatatt aatgaacttt aataaaattg atttagacaa ttggaagaga aaagagatat
     3241 ttaatcatta tttgaaccaa caaacgactt ttagtataac cacagaaatt gatattagtg
     3301 ttttataccg aaacataaaa caagaaggat ataaatttta ccctgcattt attttcttag
     3361 tgacaagggt gataaactca aatacagctt ttagaactgg ttacaatagc gacggagagt
     3421 taggttattg ggataagtta gagccacttt atacaatttt tgatggtgta tctaaaacat
     3481 tctctggtat ttggactcct gtaaagaatg acttcaaaga gttttatgat ttataccttt
     3541 ctgatgtaga gaaatataat ggttcgggga aattgtttcc caaaacacct atacctgaaa
     3601 atgctttttc tctttctatt attccatgga cttcatttac tgggtttaac ttaaatatca
     3661 ataataatag taattacctt ctacccatta ttacagcagg aaaattcatt aataaaggta
     3721 attcaatata tttaccgcta tctttacagg tacatcattc tgtttgtgat ggttatcatg
     3781 caggattgtt tatgaactct attcaggaat tgtcagatag gcctaatgac tggcttttat
     3841 aataaaggag gtcgacgtca tggaaaattt atcaaaagac atcgatgaaa ttttgatcac
     3901 agaagaagaa cttaaggaaa agataaaaga gcttgggagg caaatcacaa aagactacaa
     3961 agggaaaaat ttgatgttgg taggagtttt aaaaggtgct ttaatgttta tggctgattt
     4021 gtcaagacac atagatttgc ctttatcact tgattttatg gctgtttcca gctatggaag
     4081 ctcaactcat tcatcaggaa tagtaaagat aatcaaagat cttgatataa gcatagaagg
     4141 caaagatgtt ctgattgtgg aagacataat tgacagcggt ttgactttgt cttacttaag
     4201 ggaaacttta cttggaagga agccaaaaag cctgaaaata tgcacaatat tagacaaacc
     4261 ggagagaaga gaagcatctg taaaagtcga ttatgtagga tttaagatac ctgataagtt
     4321 tgtcgtgggt tatggattgg actttgatga aaagtacagg aaccttcctt ttataggcgt
     4381 tttgaaacct gaaatgtaca gctaagcccg ggcctcgaga aaacaaaagg ctcagtcgga
     4441 agactgggcc ttttgttttg gtaccgaatt cggcgcgcct cagcgtttaa acccgctgat
     4501 cctagagggc cgcatcatgt aattagttat gtcacgctta cattcacgcc ctccccatgc
     4561 tttaatacat acctggccca gtagttcagt gaaaaggaat ttttggacag actggacgaa
     4621 atggtggaac aggcttttga cgaccagtgc acaggtacaa atccaagata cccgcttatg
     4681 aatgaaatca ggcaaatgta tctgaacgct tattacggag gtgcgaagaa atgaagcttg
     4741 aaaaggttat agcggtaaga acggacaaaa ccgtttacag agacggagac aaggcaataa
     4801 aggtcttttc cagcaactat tcaaaggcca acgtattgaa tgaagcttta aaccaggcca
     4861 gggtggaaga aaccgggctt aacattccga agcttcttga agttacaaaa attgatggca
     4921 aatgggcaat tgtgacggag tttattgaag gaaagacctt agagcagctt atgaaagaaa
     4981 atcccttaaa atatgatgaa tatatgaacc tctttgttga tattcagctc gaagtgttaa
     5041 gcaaaagggc tcctttatta aacaagctga aagacaaaat gcacagaaaa ctgctaatag
     5101 tagtgaaaaa atcagggata atgccgctct aaccgaaaag gaaggagtta gacaacctga
     5161 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
     5221 tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac
     5281 cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcaagctgc ggccctgcat
     5341 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5401 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5461 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5521 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5581 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5641 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5701 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5761 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5821 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5881 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5941 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6001 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6061 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6121 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6181 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaaaaaa
     6241 tccgcttaag tccgcgggac ttcatggcac tttctacacc ttgcttcata agactccttt
     6301 gctccaacca ttataatggg atcgtcgtaa ttggcaggct tcccatttat aagcctttgc
     6361 gtgcgagtag caggattgcc acacttcatg catatagcag taagcttatc gacaaattca
     6421 gctatggcca tcaattctgg agtcggacca aatggttcac ctctaaagtc catgtcaagc
     6481 cctgcacata tgactctttt cccgctatcg gcaatctctt ttacgatgtc gactatttca
     6541 gaatcaaaaa attgaacttc atctatagca aatacatccg tatcttcttc agcataagcc
     6601 aatatgtcag aagcctttac tatggcaatg gcgtgcatgt tgtcgccgtt atgagatacg
     6661 actttgtcta tggaatacct atcgtctata gccggtttga aaacctgaac tttttgcctg
     6721 gcaatcttag ctctctttat ccttcttata agttcctcac tttttccact gaacatggga
     6781 ccagttacaa cttctatgta cccgtggtct ttaggcccat acatctttcg tcctccttaa
     6841 aattttcgtt tatttgtttt ttatttagtg tgcctaaaag caccatctta tacattcgta
     6901 ccttatatct cttatcttgt atctcttacc tcatatctct tattattaac taccgaagta
     6961 tattttcata acggaatctt atgacttatt tttctgactg ccgttttacc atacggttaa
     7021 tttatacgga aacagctaat ctaaccttgc tgaaggcatg tttaccaaaa tattatatct
     7081 actttaccat attaattcaa gtggtcattt tgatatttta cgccaaaatt tttagcgttc
     7141 ctttacttga taatttatcc aaaaaccttc attcgtcaaa acaattatga tataattaat
     7201 ttatgatatg attaattaat atttacggat aactaatcat ttcaatgcaa tagatcaaca
     7261 tgcaaatcaa tatccacctt actccgaaca ttttgcacca acgaatgccc agcactatat
     7321 cccggcaagc aatataacgt ctataatttt ttatgataac gataaaatta aattttatga
     7381 aaggggtaaa agcatgagaa aaatagctgt attgttaatt actttgacgt tcttagtcac
     7441 gactcgaatt taggaggctt acttgtctgc tttcttcatt agaatcaatc cttttttaaa
     7501 agtcaatccc gtttgttgaa ctactcttta ataaaataat ttttccgttc ccaattccac
     7561 attgcaataa tagaaaatcc atcttcatcg gctttttcgt catcatctgt atgaatcaaa
     7621 tcgccttctt ctgtgtcatc aaggtttaat tttttatgta tttcttttaa caaaccacca
     7681 taggagatta accttttacg gtgtaaacct tcctccaaat cagacaaacg tttcaaattc
     7741 ttttcttcat catcggtcat aaaatccgta tcctttacag gatattttgc agtttcgtca
     7801 attgccgatt gtatatccga tttatattta tttttcggtc gaatcatttg aacttttaca
     7861 tttggatcat agtctaattt cattgccttt ttccaaaatt gaatccattg tttttgattc
     7921 acgtagtttt ctgtattctt aaaataagtt ggttccacac ataccaatac atgcatgtgc
     7981 tgattataag aattatcttt attatttatt gtcacttccg ttgcacgcat aaaaccaaca
     8041 agatttttat taattttttt atattgcatc attcggcgaa atccttgagc catatctgac
     8101 aaactcttat ttaattcttc gccatcataa acatttttaa ctgttaatgt gagaaacaac
     8161 caacgaactg ttggcttttg tttaataact tcagcaacaa ccttttgtga ctgaatgcca
     8221 tgtttcattg ctctcctcca gttgcacatt ggacaaagcc tggatttaca aaaccacact
     8281 cgatacaact ttctttcgcc tgtttcacga ttttgtttat actctaatat ttcagcacaa
     8341 tcttttactc tttcagcctt tttaaattca agaatatgca gaagttcaaa gtaatcaaca
     8401 ttagcgattt tcttttctct ccatggtctc acttttccac tttttgtctt gtccactaaa
     8461 acccttgatt tttcatctga ataaatgcta ctattaggac acataatatt aaaagaaacc
     8521 cccat
//
