LOCUS       40924_26231        7335 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJLIC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Trachtmann N, Alvarez Fong KF, Guitart Font E, Sprenger GA.
  TITLE     Construction of chromosomally encoded lacZ and gfp reporter strains 
            of Escherichia coli for global regulation of metabolism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   Trachtmann N, Alvarez Fong KF, Guitart Font E, Sprenger GA.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2016) Institute of microbiology, Stuttgart 
            university, Allmandring 31, Stuttgart 70569, Germany
REFERENCE   3  (bases 1 to 7335)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7335)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2016) Institute of microbiology, Stuttgart university, 
            Allmandring 31, Stuttgart 70569, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..179
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     regulatory      797..914
                     /label=5S terminator
                     /note="5S terminator"
                     /regulatory_class="terminator"
     terminator      917..1003
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1095..1122
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1141..1232
                     /label=AmpR promoter
     CDS             1233..2090
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2264..2852
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3038..3178)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3283..3471)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(4492..5571)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(5572..5649)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    5754..6080
                     /label=flanking arm for integration region
                     /note="flanking arm for integration region"
     protein_bind    complement(6111..6158)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(6194..6832)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /function="chloramphenicol resistance"
                     /label=cat
                     /protein_id="APZ86789.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRCLMNTTVLR"
     gene            complement(6194..6832)
                     /gene="cat"
                     /label=cat
     promoter        complement(6833..6935)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(7041..7074)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     promoter        7266..7294
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    7302..7318
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agcttatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
       61 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      121 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      181 ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc
      241 ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc
      301 ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga
      361 atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc
      421 agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct
      481 gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac
      541 gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg
      601 aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac
      661 cgactacaca aatcagcgat ttccatgttg gatccccggg taccgagctc gaattcctgt
      721 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
      781 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
      841 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
      901 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      961 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     1021 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     1081 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     1141 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1201 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1261 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1321 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1381 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1441 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     1501 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1561 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1621 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1681 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1741 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1801 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1861 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1921 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     1981 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2041 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2101 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2161 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2401 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2641 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2821 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2881 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2941 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3001 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     3061 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     3121 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     3181 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     3241 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     3301 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     3361 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     3421 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca cttgatgcct
     3481 ccgtgtaagg gggaatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     3541 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     3601 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     3661 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     3721 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     3781 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     3841 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     3901 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc
     3961 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg
     4021 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg
     4081 aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac
     4141 cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc
     4201 cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg
     4261 aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta
     4321 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa
     4381 tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg
     4441 cgtcggccag cttgcaattc gcgctaactt acattaattg cgttgcgctc actgcccgct
     4501 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4561 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
     4621 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
     4681 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
     4741 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt
     4801 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
     4861 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
     4921 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
     4981 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     5041 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     5101 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
     5161 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
     5221 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
     5281 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
     5341 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
     5401 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     5461 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     5521 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     5581 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc
     5641 gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga attgcaagct gatccgggct
     5701 tatcgactgc acggtgcacc aatgcttctg gcgtcaggca gccatcgaga tcccaatgcg
     5761 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
     5821 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
     5881 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
     5941 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
     6001 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6061 actggaaagc gggcagtgag cagatccatt gtgtaggctg gagctgcttc gaagttccta
     6121 tactttctag agaataggaa cttcggaata ggaacttcat ttaaatggcg cgccttacgc
     6181 cccgccctgc cactcatcgc agtactgttg tattcattaa gcatctgccg acatggaagc
     6241 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     6301 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     6361 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
     6421 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     6481 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     6541 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     6601 tcattgccat acgtaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     6661 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     6721 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     6781 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     6841 ccttagctcc tgaaaatctc gacaactcaa aaaatacgcc cggtagtgat cttatttcat
     6901 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
     6961 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
     7021 ccgtcacagg taggcgcgcc gaagttccta tactttctag agaataggaa cttcggaata
     7081 ggaactaagg aggatattca tatggaccat ggctaattcc catgtcagcc gttaagatct
     7141 gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc
     7201 cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg
     7261 agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt
     7321 tcacacagga aacaa
//
