LOCUS       V015267                 6826 bp    DNA     circular SYN 11-DEC-2019
DEFINITION  Exported.
ACCESSION   V015267
VERSION     V015267
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Leech JM, Dhariwala MO, Lowe MM, Chu K, Merana GR, Cornuot C, Weckel
            A, Ma JM, Leitner EG, Gonzalez JR, Vasquez KS, Diep BA, Scharschmidt
            TC.
  TITLE     Toxin-Triggered Interleukin-1 Receptor Signaling Enables Early-Life
            Discrimination of Pathogenic versus Commensal Skin Bacteria
  JOURNAL   Cell Host Microbe (2019) In press
   PUBMED   31784259
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Scharschmidt TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Dermatology, University of California San
            Francisco, 513 Parnassus Ave, San Francisco, CA 94143, USA
REFERENCE   3  (bases 1 to 6826)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host
            Microbe (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAY-2019) Dermatology, University of California San Francisco,
            513 Parnassus Ave, San Francisco, CA 94143, USA"
FEATURES             Location/Qualifiers
     source          1..6826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..251
                     /regulatory_class="promoter"
     CDS             342..1031
                     /label="ZsGreen"
                     /note="Zoanthus green fluorescent protein (Matz et al.,
                     1999)"
     regulatory      1043..1342
                     /regulatory_class="terminator"
     rep_origin      1349..3433
     CDS             3873..4604
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     promoter        4972..5076
                     /label="AmpR promoter"
     CDS             5077..5934
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6108..6696
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcatgcctga tatttttgac taaaccaaat gctaacccag aaatacaatc actgtgtcta
       61 atgaataatt tgttttataa acactttttt gtttacttct catttttaat tagttataat
      121 taactaaata atagagcatt aaatatattt aataaaactt atttaatgca aaattatgac
      181 taacatatct ataataaata aagattagat atcaatatat tatcgggcaa atgtatcgag
      241 caagatgcat cgaattctta ggaggatgat tatttatgga agcgtggggg gctttagcaa
      301 attgggcagt tgatagtgcg ggtagtggca gtggatctgg tgcacaatca aaacatggtt
      361 taacaaaaga aatgacaatg aaatacagaa tggaaggctg cgttgacggt cacaaatttg
      421 taattactgg tgagggaatt ggttatccgt ttaagggaaa acaagctatt aacttatgtg
      481 tagttgaggg tggaccctta ccttttgccg aagatatact ttctgcagca tttatgtatg
      541 gaaatcgcgt atttaccgaa tatcctcaag atattgctga ttactttaag aatagctgtc
      601 ctgctggtta tacatgggat cgtagtttct tatttgaaga tggcgctgta tgtatttgta
      661 atgcagatat cactgtttcc gtcgaagaaa attgtatgta tcatgaatct aaattctatg
      721 gtgttaactt tccagctgat ggacctgtca tgaagaaaat gactgataat tgggaaccaa
      781 gctgtgagaa aatcattcca gtgccaaaac aaggtatatt gaaaggtgac gtttctatgt
      841 atttgctctt aaaagatggg ggtcgacttc gttgtcaatt tgacacagtg tataaagcaa
      901 aatcagtacc aagaaaaatg ccagattggc atttcataca acataaacta acgcgtgaag
      961 atagatcgga tgctaagaat cagaaatggc atttaacaga acatgcaatt gcttcaggat
     1021 cagccttacc ttaaggcgcg cctattctaa tgcataataa atactgataa catcttatat
     1081 tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt
     1141 ccctctatta ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta
     1201 tatacaaaaa attataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa
     1261 aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc
     1321 tcatatatca agcaaagtga caggcgcctt tgcggaaaga gttaataagt taacagaaga
     1381 tgaaccaaaa ctaaatggtt tagcaggaaa cttagataaa aaaatgaatc cagaattata
     1441 ttcagaacag gaacagcaac aagaacaaca aaagaatcaa aaacgagata gaggtatgca
     1501 cttatagaac atgcatttat gccgagaaaa cttattggtt ggaatgggct atgtgttagc
     1561 taacttgtta gcgagttggt tggacttgaa ttgggattaa tcccaagaaa gtaccaactc
     1621 aacaacacat aaagccctgt aggttccgac caataaggaa attggaataa agcaataaaa
     1681 ggagttgaag aaatgaaatt cagagaagcc tttgagaatt ttataacaag taagtatgta
     1741 cttggtgttt tagtagtttt aactgtttac cagataatac aaatgcttaa ataaaaaaag
     1801 acttgatctg attagaccaa atcttttgat agtgttatat taataacaaa ataaaaagga
     1861 gtcgctcacg ccctgaccaa agtttgtgaa cgacatcatt caaagaaaaa aacactgagt
     1921 tgtttttata atcttgtata tttagatatt aaacgatatt taaatataca tcaagatata
     1981 tatttgggtg agcgattcct taaacgaaat tgagattaag gagtcgattt tttatgtata
     2041 aaaacaatca tgcaaatcat tcaaatcatt tggaaaatca cgatttagac aatttttcta
     2101 aaaccggcta ctctaatagc cggttggacg cacatactgt gtgcatatct gatccaaaat
     2161 taagttttga tgcaatgacg atcgttggaa atctcaaccg agacaacgct caagcccttt
     2221 ctaaatttat gagtgtagag ccccaaataa gactttggga tattcttcaa acaaagttta
     2281 aagctaaagc acttcaagaa aaagtttata ttgaatatga caaagtgaaa gcagatagtt
     2341 gggatagacg taatatgcgt attgaattta atccaaacaa acttacacga gatgaaatga
     2401 tttggttaaa acaaaatata ataagctaca tggaagatga cggttttaca agattagatt
     2461 tagcctttga ttttgaagat gatttgagtg actactatgc aatgtctgat aaagcagtta
     2521 agaaaactat tttttatggt cgtaatggta agccagaaac aaaatatttt ggcgtgagag
     2581 atagtaatag atttattaga atttataata aaaagcaaga acgtaaagat aatgcagatg
     2641 ctgaagttat gtctgaacat ttatggcgtg tagaaatcga acttaaaaga gatatggtgg
     2701 attactggaa tgattgcttt agtgatttac atatcttgca accagattgg aaaactatcc
     2761 aacgcactgc ggatagagca atagttttta tgttattgag tgatgaagaa gaatggggaa
     2821 agcttcacag aaattctaga acaaaatata agaatttgat aaaagaaatt tcgccagtcg
     2881 atttaacgga cttaatgaaa tcgactttaa aagcgaacga aaaacaattg caaaaacaaa
     2941 tcgatttttg gcaacatgaa tttaaatttt ggaaatagtg tacatattaa tattactgaa
     3001 caaaaatgat atatttaaac tattctaatt taggaggatt tttttatgaa gtgtctattt
     3061 aaaaatttgg ggaatttata tgaggtgaaa gaataattta cccctataaa ctttagtcac
     3121 ctcaagtaaa gaggtaaaat tgtttagttt atataaaaaa tttaaaggtt tgttttatag
     3181 cgttttattt tggctttgta ttctttcatt ttttagtgta ttaaatgaaa tggttttaaa
     3241 tgtttcttta cctgatattg caaatcattt taatactact cctggaatta caaactgggt
     3301 aaacactgca tatatgttaa ctttttcgat aggaacagca gtatatggaa aattatctga
     3361 ttatataaat ataaaaaaat tgttaattat tggtattagt ttgagctgtc ttggttcatt
     3421 gattgctttt attgggccca cctaggaatt gaatgagaca tgctacacct ccggataata
     3481 aatatatata aacgtatata gatttcataa agtctaacac actagactta tttacttcgt
     3541 aattaagtcg ttaaaccgtg tgctctacga ccaaaactat aaaaccttta agaactttct
     3601 ttttttacaa gaaaaaagaa attagataaa tctctcatat cttttattca ataatcgcat
     3661 ccgattgcag tataaattta acgatcactc atcatgttca tatttatcag agctcgtgct
     3721 ataattatac taattttata aggaggaaaa aatatgggca tttttagtat ttttgtaatc
     3781 agcacagttc attatcaacc aaacaaaaaa taagtggtta taatgaatcg ttaataagca
     3841 aaattcatat aaccaaatta aagagggtta taatgaacga gaaaaatata aaacacagtc
     3901 aaaactttat tacttcaaaa cataatatag ataaaataat gacaaatata agattaaatg
     3961 aacatgataa tatctttgaa atcggctcag gaaaaggcca ttttaccctt gaattagtaa
     4021 agaggtgtaa tttcgtaact gccattgaaa tagaccataa attatgcaaa actacagaaa
     4081 ataaacttgt tgatcacgat aatttccaag ttttaaacaa ggatatattg cagtttaaat
     4141 ttcctaaaaa ccaatcctat aaaatatatg gtaatatacc ttataacata agtacggata
     4201 taatacgcaa aattgttttt gatagtatag ctaatgagat ttatttaatc gtggaatacg
     4261 ggtttgctaa aagattatta aatacaaaac gctcattggc attactttta atggcagaag
     4321 ttgatatttc tatattaagt atggttccaa gagaatattt tcatcctaaa cctaaagtga
     4381 atagctcact tatcagatta agtagaaaaa aatcaagaat atcacacaaa gataaacaaa
     4441 agtataatta tttcgttatg aaatgggtta acaaagaata caagaaaata tttacaaaaa
     4501 atcaatttaa caattcctta aaacatgcag gaattgacga tttaaacaat attagctttg
     4561 aacaattctt atctcttttc aatagctata aattatttaa taagtaagtt aagggatgca
     4621 taaactgcat cccttaactt gtttttcgtg tgcctatttt ttgtgaatcg attatgtctt
     4681 ttgcgcagtc ggcttaaacc agttttccgc ggcgctcgag cggccgcata gttaagccag
     4741 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4801 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4861 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4921 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4981 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5041 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5101 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5161 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5221 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5281 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5341 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5401 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5461 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5521 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5581 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5641 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5701 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5761 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5821 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5881 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5941 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6001 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6241 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6721 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6781 tctgtggata accgtattac cgcctttgag tgagctggcg gccgct
//
