LOCUS       V015908                 6841 bp    DNA     circular SYN 11-DEC-2019
DEFINITION  Exported.
ACCESSION   V015908
VERSION     V015908
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Leech JM, Dhariwala MO, Lowe MM, Chu K, Merana GR, Cornuot C, Weckel
            A, Ma JM, Leitner EG, Gonzalez JR, Vasquez KS, Diep BA, Scharschmidt
            TC.
  TITLE     Toxin-Triggered Interleukin-1 Receptor Signaling Enables Early-Life
            Discrimination of Pathogenic versus Commensal Skin Bacteria
  JOURNAL   Cell Host Microbe (2019) In press
   PUBMED   31784259
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Scharschmidt TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Dermatology, University of California San
            Francisco, 513 Parnassus Ave, San Francisco, CA 94143, USA
REFERENCE   3  (bases 1 to 6841)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host
            Microbe (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAY-2019) Dermatology, University of California San Francisco,
            513 Parnassus Ave, San Francisco, CA 94143, USA"
FEATURES             Location/Qualifiers
     source          1..6841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..251
                     /regulatory_class="promoter"
     CDS             342..1046
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      1043..1357
                     /regulatory_class="terminator"
     rep_origin      1364..3448
     CDS             3888..4619
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     promoter        4987..5091
                     /label="AmpR promoter"
     CDS             5092..5949
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6123..6711
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcatgcctga tatttttgac taaaccaaat gctaacccag aaatacaatc actgtgtcta
       61 atgaataatt tgttttataa acactttttt gtttacttct catttttaat tagttataat
      121 taactaaata atagagcatt aaatatattt aataaaactt atttaatgca aaattatgac
      181 taacatatct ataataaata aagattagat atcaatatat tatcgggcaa atgtatcgag
      241 caagatgcat cgaattctta ggaggatgat tatttatgga agcatgggga gctttagcaa
      301 attgggcagt tgattcagct ggttcaggtt caggttcagg tgtgagcaag ggcgaggagg
      361 ataatatggc tattatcaaa gaatttatga gattcaaagt tcatatggaa ggttcagtca
      421 atggtcacga atttgaaatt gagggagaag gtgaaggcag accatatgaa ggtactcaaa
      481 cggccaaact taaagtcact aaaggaggtc ctcttccatt tgcatgggat attcttagtc
      541 cacaatttat gtatggatct aaagcttacg ttaaacatcc agcggacatt ccggattatc
      601 ttaaattgtc gtttccagaa ggcttcaaat gggaaagagt aatgaacttt gaagatggag
      661 gagtcgttac agtcactcag gatagttctc ttcaagatgg agaatttatc tacaaagtca
      721 agttaagagg taccaatttt ccctcagatg gtccagttat gcaaaagaaa actatgggat
      781 gggaagcatc cagtgagcgt atgtatcctg aagatggtgc ccttaaaggc gaaattaaac
      841 aacgcctaaa gcttaaagat ggtggacatt atgacgctga agttaaaaca acttacaaag
      901 cgaagaaacc ggtacaactt ccaggtgcat ataatgtcaa cattaaactt gatattacaa
      961 gtcataatga agattatact attgttgaac aatatgagag agctgaagga agacattcta
     1021 caggcggcat ggacgagctg tacaagtaag gcgcgcctat tctaatgcat aataaatact
     1081 gataacatct tatattttgt attatatttt gtattatcgt tgacatgtat aattttgata
     1141 tcaaaaactg attttccctc tattattttc gagatttatt ttcttaattc tctttaacaa
     1201 actagaaata ttgtatatac aaaaaattat aaataataga tgaatagttt aattataggt
     1261 gttcatcaat cgaaaaagca acgtatctta tttaaagtgc gttgcttttt tctcatttat
     1321 aaggttaaat aattctcata tatcaagcaa agtgacaggc gcctttgcgg aaagagttaa
     1381 taagttaaca gaagatgaac caaaactaaa tggtttagca ggaaacttag ataaaaaaat
     1441 gaatccagaa ttatattcag aacaggaaca gcaacaagaa caacaaaaga atcaaaaacg
     1501 agatagaggt atgcacttat agaacatgca tttatgccga gaaaacttat tggttggaat
     1561 gggctatgtg ttagctaact tgttagcgag ttggttggac ttgaattggg attaatccca
     1621 agaaagtacc aactcaacaa cacataaagc cctgtaggtt ccgaccaata aggaaattgg
     1681 aataaagcaa taaaaggagt tgaagaaatg aaattcagag aagcctttga gaattttata
     1741 acaagtaagt atgtacttgg tgttttagta gttttaactg tttaccagat aatacaaatg
     1801 cttaaataaa aaaagacttg atctgattag accaaatctt ttgatagtgt tatattaata
     1861 acaaaataaa aaggagtcgc tcacgccctg accaaagttt gtgaacgaca tcattcaaag
     1921 aaaaaaacac tgagttgttt ttataatctt gtatatttag atattaaacg atatttaaat
     1981 atacatcaag atatatattt gggtgagcga ttccttaaac gaaattgaga ttaaggagtc
     2041 gattttttat gtataaaaac aatcatgcaa atcattcaaa tcatttggaa aatcacgatt
     2101 tagacaattt ttctaaaacc ggctactcta atagccggtt ggacgcacat actgtgtgca
     2161 tatctgatcc aaaattaagt tttgatgcaa tgacgatcgt tggaaatctc aaccgagaca
     2221 acgctcaagc cctttctaaa tttatgagtg tagagcccca aataagactt tgggatattc
     2281 ttcaaacaaa gtttaaagct aaagcacttc aagaaaaagt ttatattgaa tatgacaaag
     2341 tgaaagcaga tagttgggat agacgtaata tgcgtattga atttaatcca aacaaactta
     2401 cacgagatga aatgatttgg ttaaaacaaa atataataag ctacatggaa gatgacggtt
     2461 ttacaagatt agatttagcc tttgattttg aagatgattt gagtgactac tatgcaatgt
     2521 ctgataaagc agttaagaaa actatttttt atggtcgtaa tggtaagcca gaaacaaaat
     2581 attttggcgt gagagatagt aatagattta ttagaattta taataaaaag caagaacgta
     2641 aagataatgc agatgctgaa gttatgtctg aacatttatg gcgtgtagaa atcgaactta
     2701 aaagagatat ggtggattac tggaatgatt gctttagtga tttacatatc ttgcaaccag
     2761 attggaaaac tatccaacgc actgcggata gagcaatagt ttttatgtta ttgagtgatg
     2821 aagaagaatg gggaaagctt cacagaaatt ctagaacaaa atataagaat ttgataaaag
     2881 aaatttcgcc agtcgattta acggacttaa tgaaatcgac tttaaaagcg aacgaaaaac
     2941 aattgcaaaa acaaatcgat ttttggcaac atgaatttaa attttggaaa tagtgtacat
     3001 attaatatta ctgaacaaaa atgatatatt taaactattc taatttagga ggattttttt
     3061 atgaagtgtc tatttaaaaa tttggggaat ttatatgagg tgaaagaata atttacccct
     3121 ataaacttta gtcacctcaa gtaaagaggt aaaattgttt agtttatata aaaaatttaa
     3181 aggtttgttt tatagcgttt tattttggct ttgtattctt tcatttttta gtgtattaaa
     3241 tgaaatggtt ttaaatgttt ctttacctga tattgcaaat cattttaata ctactcctgg
     3301 aattacaaac tgggtaaaca ctgcatatat gttaactttt tcgataggaa cagcagtata
     3361 tggaaaatta tctgattata taaatataaa aaaattgtta attattggta ttagtttgag
     3421 ctgtcttggt tcattgattg cttttattgg gcccacctag gaattgaatg agacatgcta
     3481 cacctccgga taataaatat atataaacgt atatagattt cataaagtct aacacactag
     3541 acttatttac ttcgtaatta agtcgttaaa ccgtgtgctc tacgaccaaa actataaaac
     3601 ctttaagaac tttctttttt tacaagaaaa aagaaattag ataaatctct catatctttt
     3661 attcaataat cgcatccgat tgcagtataa atttaacgat cactcatcat gttcatattt
     3721 atcagagctc gtgctataat tatactaatt ttataaggag gaaaaaatat gggcattttt
     3781 agtatttttg taatcagcac agttcattat caaccaaaca aaaaataagt ggttataatg
     3841 aatcgttaat aagcaaaatt catataacca aattaaagag ggttataatg aacgagaaaa
     3901 atataaaaca cagtcaaaac tttattactt caaaacataa tatagataaa ataatgacaa
     3961 atataagatt aaatgaacat gataatatct ttgaaatcgg ctcaggaaaa ggccatttta
     4021 cccttgaatt agtaaagagg tgtaatttcg taactgccat tgaaatagac cataaattat
     4081 gcaaaactac agaaaataaa cttgttgatc acgataattt ccaagtttta aacaaggata
     4141 tattgcagtt taaatttcct aaaaaccaat cctataaaat atatggtaat ataccttata
     4201 acataagtac ggatataata cgcaaaattg tttttgatag tatagctaat gagatttatt
     4261 taatcgtgga atacgggttt gctaaaagat tattaaatac aaaacgctca ttggcattac
     4321 ttttaatggc agaagttgat atttctatat taagtatggt tccaagagaa tattttcatc
     4381 ctaaacctaa agtgaatagc tcacttatca gattaagtag aaaaaaatca agaatatcac
     4441 acaaagataa acaaaagtat aattatttcg ttatgaaatg ggttaacaaa gaatacaaga
     4501 aaatatttac aaaaaatcaa tttaacaatt ccttaaaaca tgcaggaatt gacgatttaa
     4561 acaatattag ctttgaacaa ttcttatctc ttttcaatag ctataaatta tttaataagt
     4621 aagttaaggg atgcataaac tgcatccctt aacttgtttt tcgtgtgcct attttttgtg
     4681 aatcgattat gtcttttgcg cagtcggctt aaaccagttt tccgcggcgc tcgagcggcc
     4741 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4801 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4861 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4921 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4981 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5041 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5101 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5161 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5221 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5281 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5341 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5401 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5461 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5521 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5581 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5641 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5701 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5761 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5821 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5881 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5941 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6001 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6061 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6241 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6721 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6781 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tggcggccgc
     6841 t
//
