LOCUS       V016150                 6760 bp    DNA     circular SYN 11-DEC-2019
DEFINITION  Exported.
ACCESSION   V016150
VERSION     V016150
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Leech JM, Dhariwala MO, Lowe MM, Chu K, Merana GR, Cornuot C, Weckel
            A, Ma JM, Leitner EG, Gonzalez JR, Vasquez KS, Diep BA, Scharschmidt
            TC.
  TITLE     Toxin-Triggered Interleukin-1 Receptor Signaling Enables Early-Life
            Discrimination of Pathogenic versus Commensal Skin Bacteria
  JOURNAL   Cell Host Microbe (2019) In press
   PUBMED   31784259
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Scharschmidt TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Dermatology, University of California San
            Francisco, 513 Parnassus Ave, San Francisco, CA 94143, USA
REFERENCE   3  (bases 1 to 6760)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host
            Microbe (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAY-2019) Dermatology, University of California San Francisco,
            513 Parnassus Ave, San Francisco, CA 94143, USA"
FEATURES             Location/Qualifiers
     source          1..6760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..251
                     /regulatory_class="promoter"
     CDS             342..1046
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      1058..1357
                     /regulatory_class="terminator"
     rep_origin      1364..3448
     CDS             3653..4300
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     promoter        4906..5010
                     /label="AmpR promoter"
     CDS             5011..5868
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6042..6630
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcatgcctga tatttttgac taaaccaaat gctaacccag aaatacaatc actgtgtcta
       61 atgaataatt tgttttataa acactttttt gtttacttct catttttaat tagttataat
      121 taactaaata atagagcatt aaatatattt aataaaactt atttaatgca aaattatgac
      181 taacatatct ataataaata aagattagat atcaatatat tatcgggcaa atgtatcgag
      241 caagatgcat cgaattctta ggaggatgat tatttatgga agcatgggga gctttagcaa
      301 attgggcagt tgattcagct ggttcaggtt caggttcagg tgtgagcaag ggcgaggagg
      361 ataatatggc tattatcaaa gaatttatga gattcaaagt tcatatggaa ggttcagtca
      421 atggtcacga atttgaaatt gagggagaag gtgaaggcag accatatgaa ggtactcaaa
      481 cggccaaact taaagtcact aaaggaggtc ctcttccatt tgcatgggat attcttagtc
      541 cacaatttat gtatggatct aaagcttacg ttaaacatcc agcggacatt ccggattatc
      601 ttaaattgtc gtttccagaa ggcttcaaat gggaaagagt aatgaacttt gaagatggag
      661 gagtcgttac agtcactcag gatagttctc ttcaagatgg agaatttatc tacaaagtca
      721 agttaagagg taccaatttt ccctcagatg gtccagttat gcaaaagaaa actatgggat
      781 gggaagcatc cagtgagcgt atgtatcctg aagatggtgc ccttaaaggc gaaattaaac
      841 aacgcctaaa gcttaaagat ggtggacatt atgacgctga agttaaaaca acttacaaag
      901 cgaagaaacc ggtacaactt ccaggtgcat ataatgtcaa cattaaactt gatattacaa
      961 gtcataatga agattatact attgttgaac aatatgagag agctgaagga agacattcta
     1021 caggcggcat ggacgagctg tacaagtaag gcgcgcctat tctaatgcat aataaatact
     1081 gataacatct tatattttgt attatatttt gtattatcgt tgacatgtat aattttgata
     1141 tcaaaaactg attttccctc tattattttc gagatttatt ttcttaattc tctttaacaa
     1201 actagaaata ttgtatatac aaaaaattat aaataataga tgaatagttt aattataggt
     1261 gttcatcaat cgaaaaagca acgtatctta tttaaagtgc gttgcttttt tctcatttat
     1321 aaggttaaat aattctcata tatcaagcaa agtgacaggc gcctttgcgg aaagagttaa
     1381 taagttaaca gaagatgaac caaaactaaa tggtttagca ggaaacttag ataaaaaaat
     1441 gaatccagaa ttatattcag aacaggaaca gcaacaagaa caacaaaaga atcaaaaacg
     1501 agatagaggt atgcacttat agaacatgca tttatgccga gaaaacttat tggttggaat
     1561 gggctatgtg ttagctaact tgttagcgag ttggttggac ttgaattggg attaatccca
     1621 agaaagtacc aactcaacaa cacataaagc cctgtaggtt ccgaccaata aggaaattgg
     1681 aataaagcaa taaaaggagt tgaagaaatg aaattcagag aagcctttga gaattttata
     1741 acaagtaagt atgtacttgg tgttttagta gttttaactg tttaccagat aatacaaatg
     1801 cttaaataaa aaaagacttg atctgattag accaaatctt ttgatagtgt tatattaata
     1861 acaaaataaa aaggagtcgc tcacgccctg accaaagttt gtgaacgaca tcattcaaag
     1921 aaaaaaacac tgagttgttt ttataatctt gtatatttag atattaaacg atatttaaat
     1981 atacatcaag atatatattt gggtgagcga ttccttaaac gaaattgaga ttaaggagtc
     2041 gattttttat gtataaaaac aatcatgcaa atcattcaaa tcatttggaa aatcacgatt
     2101 tagacaattt ttctaaaacc ggctactcta atagccggtt ggacgcacat actgtgtgca
     2161 tatctgatcc aaaattaagt tttgatgcaa tgacgatcgt tggaaatctc aaccgagaca
     2221 acgctcaagc cctttctaaa tttatgagtg tagagcccca aataagactt tgggatattc
     2281 ttcaaacaaa gtttaaagct aaagcacttc aagaaaaagt ttatattgaa tatgacaaag
     2341 tgaaagcaga tagttgggat agacgtaata tgcgtattga atttaatcca aacaaactta
     2401 cacgagatga aatgatttgg ttaaaacaaa atataataag ctacatggaa gatgacggtt
     2461 ttacaagatt agatttagcc tttgattttg aagatgattt gagtgactac tatgcaatgt
     2521 ctgataaagc agttaagaaa actatttttt atggtcgtaa tggtaagcca gaaacaaaat
     2581 attttggcgt gagagatagt aatagattta ttagaattta taataaaaag caagaacgta
     2641 aagataatgc agatgctgaa gttatgtctg aacatttatg gcgtgtagaa atcgaactta
     2701 aaagagatat ggtggattac tggaatgatt gctttagtga tttacatatc ttgcaaccag
     2761 attggaaaac tatccaacgc actgcggata gagcaatagt ttttatgtta ttgagtgatg
     2821 aagaagaatg gggaaagctt cacagaaatt ctagaacaaa atataagaat ttgataaaag
     2881 aaatttcgcc agtcgattta acggacttaa tgaaatcgac tttaaaagcg aacgaaaaac
     2941 aattgcaaaa acaaatcgat ttttggcaac atgaatttaa attttggaaa tagtgtacat
     3001 attaatatta ctgaacaaaa atgatatatt taaactattc taatttagga ggattttttt
     3061 atgaagtgtc tatttaaaaa tttggggaat ttatatgagg tgaaagaata atttacccct
     3121 ataaacttta gtcacctcaa gtaaagaggt aaaattgttt agtttatata aaaaatttaa
     3181 aggtttgttt tatagcgttt tattttggct ttgtattctt tcatttttta gtgtattaaa
     3241 tgaaatggtt ttaaatgttt ctttacctga tattgcaaat cattttaata ctactcctgg
     3301 aattacaaac tgggtaaaca ctgcatatat gttaactttt tcgataggaa cagcagtata
     3361 tggaaaatta tctgattata taaatataaa aaaattgtta attattggta ttagtttgag
     3421 ctgtcttggt tcattgattg cttttattgg gccccccatt atgctttggc agtttattct
     3481 tgacatgtag tgagggggct ggtataatca catacggccg ataaagcaag catataatat
     3541 tgcgtttcat ctttagaagc gaatttcgcc aatattataa ttatcaaaga gaggggtggc
     3601 aaacggtatt tggcattatt aggttaaaaa atgtagaagg agagtgaaac ccatgaactt
     3661 taataaaatt gatttagaca attggaagag aaaagagata tttaatcatt atttgaacca
     3721 acaaacgact tttagtataa ccacagaaat tgatattagt gttttatacc gaaacataaa
     3781 acaagaagga tataaatttt accctgcatt tattttctta gtgacaaggg tgataaactc
     3841 aaatacagct tttagaactg gttacaatag cgacggagag ttaggttatt gggataagtt
     3901 agagccactt tatacaattt ttgatggtgt atctaaaaca ttctctggta tttggactcc
     3961 tgtaaagaat gacttcaaag agttttatga tttatacctt tctgatgtag agaaatataa
     4021 tggttcgggg aaattgtttc ccaaaacacc tatacctgaa aatgcttttt ctctttctat
     4081 tattccatgg acttcattta ctgggtttaa cttaaatatc aataataata gtaattacct
     4141 tctacccatt attacagcag gaaaattcat taataaaggt aattcaatat atttaccgct
     4201 atctttacag gtacatcatt ctgtttgtga tggttatcat gcaggattgt ttatgaactc
     4261 tattcaggaa ttgtcagata ggcctaatga ctggctttta taatatgaga taatgccgac
     4321 tgtacttttt acagtcggtt ttctaatgtc actaacctgc cccgttagtt gacatatgat
     4381 catcataatt ctgtctcatt atataacatc ctccatacct tctattatag aatgtattgc
     4441 tattaatcgc aacatcaaac caaaataaaa acccccttcg actttcgtca gggggctttt
     4501 atttattcaa taatccctcc tctcaataaa tctattgttg tacttaattc aacttccatt
     4561 tctctgtatc tttcaatacg ctcttttaag tccttaattt ctttttttaa ttcctcattt
     4621 tcagcaaata actctttttc tttgtttgct cgagcggccg catagttaag ccagccccga
     4681 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4741 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4801 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4861 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4921 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4981 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5041 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5101 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5161 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5221 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5281 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5341 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5401 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5461 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5521 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5581 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5641 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5701 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5761 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5821 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5881 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5941 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6001 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6061 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6121 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6181 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6241 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6301 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6361 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6421 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6481 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6541 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6601 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6661 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6721 gataaccgta ttaccgcctt tgagtgagct ggcggccgct
//
