LOCUS       40924_26221        8765 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Artificial vector pJL541, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Patel RD, Lodge JK, Baker LG.
  TITLE     Going green in Cryptococcus neoformans: the recycling of a 
            selectable drug marker
  JOURNAL   Fungal Genet. Biol. 47 (3), 191-198 (2010)
  PUBMED    19944774
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   Patel RD, Lodge JK, Baker LG.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2011) Molecular Microbiology, Washington 
            University, 660 S Euclid Ave, St. Louis, MO 63110, USA
REFERENCE   3  (bases 1 to 8765)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8765)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2010"; volume: "47"; issue: "3"; pages: 
            "191-198"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2011) Molecular Microbiology, Washington University, 660 S 
            Euclid Ave, St. Louis, MO 63110, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1117..1908
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      2456..3413
                     /label=Cryptococcus neoformans GAL7 promoter
                     /note="Cryptococcus neoformans GAL7 promoter"
                     /regulatory_class="promoter"
     CDS             3414..4442
                     /label=Cre
                     /note="site-specific recombinase"
     regulatory      4446..5028
                     /label=Cryptococcus neoformans GAPDH terminator
                     /note="Cryptococcus neoformans GAPDH terminator"
                     /regulatory_class="terminator"
     misc_feature    5124..5145
                     /label=PCR primer binding site 5/6
                     /note="PCR primer binding site 5/6"
     promoter        complement(5199..5217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5224..5240)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5381..5809
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             6153..6944
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             6965..7822
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7996..8584
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttaggatg
      301 tgagctggag agcggggcac gggaagggga ggactcacat aagcatgcag gattcgagtg
      361 gcatggtgtg cgctgagtgt atggttgtcg gaggagagga tgatggtaac aacaatagca
      421 gcaacgtcac tcgacgcgcg tccggtgtgc cacacggggt aacgccgagt cgccgtcagg
      481 gtcgccgaga ccactctcac agcgtcaccg ttggcaccag ctcagcttac agcttctatc
      541 ctccgccagc atccacatac atcccctata ccgcatcccc cacccactgc ccaaggtgag
      601 tcatcttccc gcccccttcc cttgcccgcc actcagtcct ccatcctcca ctaatccacc
      661 ttatcgcacc caccgcctat cgcacatccg agcacaatgc tgggcctgcc aggggctgct
      721 agatggtgct ctccccacgc tgatctgcat gccggccatt ggatcatggg tgctaggtgc
      781 tgggtgctgg atgttggatg ctggatgctg ggtgcacgct tggtcatttc cttccaggat
      841 tgacggtcgc cgagaggacg acgtggcgtt cgacaacgag ggccgatagc accgcatcgc
      901 ctcgacctgc atccatctgc cttgtccttt tggtggaaca atccatccgt gctggtgcca
      961 cacgcatagc tggaagagat ggatgtgcgt tgaacagagc tgccgtcagg actttttggt
     1021 gcacggaccc tattgtcctc ccaatcttca ccgcgtctcc taatatgcag cctctttgct
     1081 aattgtcttt ttccattagt aaactcgccc ggatccatga ttgaacaaga tggattgcac
     1141 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     1201 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     1261 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     1321 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     1381 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     1441 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     1501 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     1561 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     1621 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     1681 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     1741 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     1801 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     1861 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agaattcccc
     1921 ctcaaacccc gtctcttcat tctctcaatt tctctcgacc gcaacaagcg gatagacagt
     1981 cttcttgaca ttcttgatac attgctaacg ctgtgctatg acatgcatat tttgcccaag
     2041 tacaaaagtg tcactcgaca ctaattgtaa tttcccattt acaacgggag acaagaacct
     2101 gcaaacatgg acatacacaa tgtaaaacat tccaaggaaa aaaaggtaga aggagtctga
     2161 ttttacagtc ctgtagcttg aatgatgtac actaacccaa gaatgaacac cgcctctgcc
     2221 aacaagagtc tccactgttt cctcatccag ccgagcatct tgaactggga ttgatcacgc
     2281 ccaaaattca aatcgcgtca gcgcccttta tcccggaaac gacgatcaaa gcattcgaat
     2341 ggaaaatgac agcagatggg aatcagactt accgcgggta catctggtcc aaaaggaagc
     2401 ccttcaaagg caatttcaaa ccatggcgtg gacggcgatt ctagctcgtc ttctttatga
     2461 agtctctttt tcttcttctt ggacgaggag gaggcagagg agcggcggtg tcttatttgn
     2521 ggggccttgt gtgcgtgctt tcttttcttt tggatttttc gtccgcggga cgagtttggt
     2581 cgctctctgt accatgcttg tgagtttgtt tatgcctccg tttccccttg cttctagtct
     2641 cttccttctc cacaactttt ccatcttctt cttcttcttc tcctcctttt tcatcctcat
     2701 ttccatctac ctcttcttcc acttttttta aactttttcc atcgctatcc cttntggggt
     2761 tttcccaacc ggttcgggac tctcaaaacc ccagaatcac cagagtcctg ctttcctccc
     2821 tcttcaccct cttgtcggat atcatctgtc cccccgatag aagcaggtct tgtcgaaccc
     2881 cggggatgcg acagcagagg ttcgaacacg ccacatccat ttgatattta tatgcgcgcc
     2941 caccttcgct gacaattccc gttcatgaat cgtttgtcag taattgacgc gccgggggtg
     3001 ccttggggga gtgttgtcaa ttaacataca aatattcccg ttcgtatcaa tttgcatatc
     3061 catctcatca catagtagta ttcccgttcg tatccatttg catatctcgt cacatagtct
     3121 tcccgttcgt atccatttca tatcgtaaca tcatacagtc ttcacaatct tcccgttcgt
     3181 cctctcatcg tcaagccatt gattgtcaat acacgcctac atattcccgt tctcttgtct
     3241 gccacatcgc tcgtgcgttc ttcccgtttt tatgggaaga ctggggtcat gtcgtcaata
     3301 cagcagcaga cataatgggt gttatatatg tcgagtttca catctggctg tactaagata
     3361 tccgtgaccc actttcacaa cgatcactta tcagcgctca atcccctctt gcaatgtcca
     3421 atttactgac cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg
     3481 ttcgcaagaa cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga
     3541 aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat
     3601 ggtttcccgc agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc
     3661 tggcagtaaa aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg
     3721 ggctgccacg accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag
     3781 aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg
     3841 accaggttcg ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat
     3901 ttctggggat tgcttataac accctgttac gtatagccga aattgccagg atcagggtta
     3961 aagatatctc acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc
     4021 tggttagcac cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc
     4081 gatggatttc cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca
     4141 gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag
     4201 ggatttttga agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat
     4261 acctggcctg gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg
     4321 gagtttcaat accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga
     4381 actatatccg taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg
     4441 attagaggct ggtattgctc ttaatgccaa ctttgtcaaa cttgttagct gggtatgttg
     4501 cgtccttgtt tgttcctggt ttgtcaacat tggctgatat tttttagtat gacaatgagt
     4561 acggttactc taggcgtgtc tgcgacctca tttcttacat tgccggtgtc gatgccaagg
     4621 ctcagtagag ggggttacag tagcagaata gcagttgtct gttaatcaga agacataaac
     4681 attaaaagaa tttttcgatg ttgactatgc atatggtgga tgaagaacgt gcatacatgt
     4741 accccaactg cgtggcggct gttaaatttt gtaaacgtgt gaaaatgccg aaagcaaaat
     4801 gcgaaaatga gaaatgaagg ggaactattt aggacgcccc ctatttcttt cgggacatgc
     4861 ccctcctact gctttgggta attttgacac atggccgaga gataacacca ttgaccctgt
     4921 agggcatccc tgagaccctt gtgcctgtta tttacgtaat taaatgttct tattctgtgc
     4981 atggtaattc actcaatcct ctattcagag ccgcctatcc agctgctttt tacactttac
     5041 atgtttattt tgataggttt gaaacacagg gaaggtttca tctgtatggt tatcgaaata
     5101 ctggagaatc agccagacaa ggatgacgaa gggcgaattc tgcagatatc catcacactg
     5161 gcggccgctc gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa
     5221 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5281 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5341 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
     5401 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5461 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5521 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5581 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5641 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5701 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5761 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaatt
     5821 cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt
     5881 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     5941 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     6001 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     6061 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     6121 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     6181 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     6241 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     6301 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     6361 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     6421 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatcccac cttgctcctg
     6481 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6541 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     6601 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     6661 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     6721 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6781 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6841 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6901 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt gaaaaaggaa
     6961 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7021 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7081 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7141 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7201 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7261 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7321 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7381 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7441 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7501 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7561 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7621 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7681 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7741 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7801 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7861 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8101 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8701 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8761 ggaag
//
