LOCUS       40924_26216        5716 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Artificial vector pJL531, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Patel RD, Lodge JK, Baker LG.
  TITLE     Going green in Cryptococcus neoformans: the recycling of a 
            selectable drug marker
  JOURNAL   Fungal Genet. Biol. 47 (3), 191-198 (2010)
  PUBMED    19944774
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Patel RD, Lodge JK, Baker LG.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2011) Molecular Microbiology, Washington 
            University, 660 S Euclid Ave, St. Louis, MO 63110, USA
REFERENCE   3  (bases 1 to 5716)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5716)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2010"; volume: "47"; issue: "3"; pages: 
            "191-198"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2011) Molecular Microbiology, Washington University, 660 S 
            Euclid Ave, St. Louis, MO 63110, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    310..343
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      354..1178
                     /label=Cryptococcus neoformans actin promoter
                     /note="Cryptococcus neoformans actin promoter"
                     /regulatory_class="promoter"
     CDS             1191..1751
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      1755..2017
                     /label=Cryptococcus neoformans Trp-1 terminator
                     /note="Cryptococcus neoformans Trp-1 terminator"
                     /regulatory_class="terminator"
     misc_feature    2018..2079
                     /label=LoxP site
                     /note="LoxP site"
     protein_bind    complement(2038..2071)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        complement(2150..2168)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2175..2191)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2332..2760
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             3104..3895
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             3916..4773
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4947..5535
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc cctttcgaca
      301 acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtct agaaggatgt
      361 gagctggaga gcggggcacg ggaaggggag gactcacata agcatgcagg attcgagtgg
      421 catggtgtgc gctgagtgta tggttgtcgg aggagaggat gatggtaaca acaatagcag
      481 caacgtcact cgacgcgcgt ccggtgtgcc acacggggta acgccgagtc gccgtcaggg
      541 tcgccgagac cactctcaca gcgtcaccgt tggcaccagc tcagcttaca gcttctatcc
      601 tccgccagca tccacataca tcccctatac cgcatccccc acccactgcc caaggtgagt
      661 catcttcccg cccccttccc ttgcccgcca ctcagtcctc catcctccac taatccacct
      721 tatcgcaccc accgcctatc gcacatccga gcacaatgct gggcctgcca ggggctgcta
      781 gatggtgctc tccccacgct gatctgcatg ccggccattg gatcatgggt gctaggtgct
      841 gggtgctgga tgttggatgc tggatgctgg gtgcacgctt ggtcatttcc ttccaggatt
      901 gacggtcgcc gagaggacga cgtggcgttc gacaacgagg gccgatagca ccgcatcgcc
      961 tcgacctgca tccatctgcc ttgtcctttt ggtggaacaa tccatccgtg ctggtgccac
     1021 acgcatagct ggaagagatg gatgtgcgtt gaacagagct gccgtcagga ctttttggtg
     1081 cacggaccct attgtcctcc caatcttcac cgcgtctcct aatatgcagc ctctttgcta
     1141 attgtctttt tccattagta aactcgccca acatgtctat ggcggccgcc actcttgacg
     1201 acacggctta ccggtaccgc accagtgtcc cgggggacgc cgaggccatc gaggcactgg
     1261 atgggtcctt caccaccgac accgtcttcc gcgtcaccgc caccggggac ggcttcaccc
     1321 tgcgggaggt gccggtggac ccgcccctga ccaaggtgtt ccccgacgac gaatcggacg
     1381 acgaatcgga cgccggggag gacggcgacc cggactcccg gacgttcgtc gcgtacgggg
     1441 acgacggcga cctggcgggc ttcgtggtcg tctcgtactc cggctggaac cgccggctga
     1501 ccgtcgagga catcgaggtc gccccggagc accgggggca cggggtcggg cgcgcgttga
     1561 tggggctcgc gacggagttc gcccgcgagc ggggcgccgg gcacctctgg ctggaggtca
     1621 ccaacgtcaa cgcaccggcg atccacgcgt accggcggat ggggttcacc ctctgcggcc
     1681 tggacaccgc cctgtacgac ggcaccgcct cggacggcga gcaggcgctc tacatgagca
     1741 tgccctgccc ctaagaattc gtgaaggcgg taaggggtta attttcctta gagggtgtat
     1801 atatacatat tagagaagtg atacaatttt agcacactgc gaatccgaga cagacatcgt
     1861 gtcaatcatc ttttttgaca ttatatgccc attaatctat ctacagacaa caataccatc
     1921 cttcccaccc tcagcaacgc cgttgaatcc tcaggatctt catggctcct tgtctctgaa
     1981 accaggaagc tagtttctac atctcttcta taagcttagc tctcgagaac ccttaatata
     2041 acttcgtata atgtatgcta tacgaagtta ttaggtagta agggcgaatt ctgcagatat
     2101 ccatcacact ggcggccgct cgagcatgca tctagagggc ccaattcgcc ctatagtgag
     2161 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     2221 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     2281 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     2341 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2401 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     2461 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2521 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     2581 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2641 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     2701 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     2761 ttaacaaaat tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc
     2821 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
     2881 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
     2941 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt
     3001 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag
     3061 gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     3121 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3181 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3241 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     3301 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3361 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatccca
     3421 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3481 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     3541 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     3601 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     3661 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     3721 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     3781 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     3841 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat
     3901 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3961 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4021 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4081 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4141 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4201 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4261 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4321 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4381 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4441 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4501 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4561 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4621 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4681 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4741 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4801 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4861 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4921 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4981 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5041 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5101 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5161 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5221 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5281 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5341 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5401 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5461 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5521 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5581 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5641 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5701 agcgaggaag cggaag
//
