LOCUS       40924_26206        7869 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Artificial vector pJL519, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Patel RD, Lodge JK, Baker LG.
  TITLE     Going green in Cryptococcus neoformans: the recycling of a 
            selectable drug marker
  JOURNAL   Fungal Genet. Biol. 47 (3), 191-198 (2010)
  PUBMED    19944774
REFERENCE   2  (bases 1 to 7869)
  AUTHORS   Patel RD, Lodge JK, Baker LG.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2011) Molecular Microbiology, Washington 
            University, 660 S Euclid Ave, St. Louis, MO 63110, USA
REFERENCE   3  (bases 1 to 7869)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7869)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2010"; volume: "47"; issue: "3"; pages: 
            "191-198"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2011) Molecular Microbiology, Washington University, 660 S 
            Euclid Ave, St. Louis, MO 63110, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1132..1692
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      1959..2517
                     /label=Cryptococcus neoformans GAL7 promoter
                     /note="Cryptococcus neoformans GAL7 promoter"
                     /regulatory_class="promoter"
     CDS             2518..3546
                     /label=Cre
                     /note="site-specific recombinase"
     regulatory      3550..4232
                     /label=Cryptococcus neoformans GAPDH terminator
                     /note="Cryptococcus neoformans GAPDH terminator"
                     /regulatory_class="terminator"
     promoter        complement(4303..4321)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4328..4344)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4485..4913
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             5257..6048
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             6069..6926
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7100..7688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttaggatg
      301 tgagctggag agcggggcac gggaagggga ggactcacat aagcatgcag gattcgagtg
      361 gcatggtgtg cgctgagtgt atggttgtcg gaggagagga tgatggtaac aacaatagca
      421 gcaacgtcac tcgacgcgcg tccggtgtgc cacacggggt aacgccgagt cgccgtcagg
      481 gtcgccgaga ccactctcac agcgtcaccg ttggcaccag ctcagcttac agcttctatc
      541 ctccgccagc atccacatac atcccctata ccgcatcccc cacccactgc ccaaggtgag
      601 tcatcttccc gcccccttcc cttgcccgcc actcagtcct ccatcctcca ctaatccacc
      661 ttatcgcacc caccgcctat cgcacatccg agcacaatgc tgggcctgcc aggggctgct
      721 agatggtgct ctccccacgc tgatctgcat gccggccatt ggatcatggg tgctaggtgc
      781 tgggtgctgg atgttggatg ctggatgctg ggtgcacgct tggtcatttc cttccaggat
      841 tgacggtcgc cgagaggacg acgtggcgtt cgacaacgag ggccgatagc accgcatcgc
      901 ctcgacctgc atccatctgc cttgtccttt tggtggaaca atccatccgt gctggtgcca
      961 cacgcatagc tggaagagat ggatgtgcgt tgaacagagc tgccgtcagg actttttggt
     1021 gcacggaccc tattgtcctc ccaatcttca ccgcgtctcc taatatgcag cctctttgct
     1081 aattgtcttt ttccattagt aaactcgccc aacatgtcta tggcggccgc cactcttgac
     1141 gacacggctt accggtaccg caccagtgtc ccgggggacg ccgaggccat cgaggcactg
     1201 gatgggtcct tcaccaccga caccgtcttc cgcgtcaccg ccaccgggga cggcttcacc
     1261 ctgcgggagg tgccggtgga cccgcccctg accaaggtgt tccccgacga cgaatcggac
     1321 gacgaatcgg acgccgggga ggacggcgac ccggactccc ggacgttcgt cgcgtacggg
     1381 gacgacggcg acctggcggg cttcgtggtc gtctcgtact ccggctggaa ccgccggctg
     1441 accgtcgagg acatcgaggt cgccccggag caccgggggc acggggtcgg gcgcgcgttg
     1501 atggggctcg cgacggagtt cgcccgcgag cggggcgccg ggcacctctg gctggaggtc
     1561 accaacgtca acgcaccggc gatccacgcg taccggcgga tggggttcac cctctgcggc
     1621 ctggacaccg ccctgtacga cggcaccgcc tcggacggcg agcaggcgct ctacatgagc
     1681 atgccctgcc cctaagaatt cgtgaaggcg gtaaggggtt aattttcctt agagggtgta
     1741 tatatacata ttagagaagt gatacaattt tagcacactg cgaatccgag acagacatcg
     1801 tgtcaatcat cttttttgac attatatgcc cattaatcta tctacagaca acaataccat
     1861 ccttcccacc ctcagcaacg ccgttgaatc ctcaggatct tcatggctcc ttgtctctga
     1921 aaccaggaag ctagtttcta catctcttct ataagcttcg atagaagcag gtcttgtcga
     1981 accccgggga tgcgacagca gaggttcgaa cacgccacat ccatttgata tttatatgcg
     2041 cgcccacctt cgctgacaat tcccgttcat gaatcgtttg tcagtaattg acgcgccggg
     2101 ggtgccttgg gggagtgttg tcaattaaca tacaaatatt cccgttcgta tcaatttgca
     2161 tatccatctc atcacatagt agtattcccg ttcgtatcca tttgcatatc tcgtcacata
     2221 gtcttcccgt tcgtatccat ttcatatcgt aacatcatac agtcttcaca atcttcccgt
     2281 tcgtcctctc atcgtcaagc cattgattgt caatacacgc ctacatattc ccgttctctt
     2341 gtctgccaca tcgctcgtgc gttcttcccg tttttatggg aagactgggg tcatgtcgtc
     2401 aatacagcag cagacataat gggtgttata tatgtcgagt ttcacatctg gctgtactaa
     2461 gatatccgtg acccactttc acaacgatca cttatcagcg ctcaatcccc tcttgcaatg
     2521 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
     2581 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
     2641 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
     2701 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
     2761 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
     2821 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
     2881 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
     2941 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg
     3001 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
     3061 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
     3121 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
     3181 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
     3241 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
     3301 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
     3361 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
     3421 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
     3481 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
     3541 ggcgattaga ggctggtatt gctcttaatg ccaactttgt caaacttgtt agctgggtat
     3601 gttgcgtcct tgtttgttcc tggtttgtca acattggctg atatttttta gtatgacaat
     3661 gagtacggtt actctaggcg tgtctgcgac ctcatttctt acattgccgg tgtcgatgcc
     3721 aaggctcagt agagggggtt acagtagcag aatagcagtt gtctgttaat cagaagacat
     3781 aaacattaaa agaatttttc gatgttgact atgcatatgg tggatgaaga acgtgcatac
     3841 atgtacccca actgcgtggc ggctgttaaa ttttgtaaac gtgtgaaaat gccgaaagca
     3901 aaatgcgaaa atgagaaatg aaggggaact atttaggacg ccccctattt ctttcgggac
     3961 atgcccctcc tactgctttg ggtaattttg acacatggcc gagagataac accattgacc
     4021 ctgtagggca tccctgagac ccttgtgcct gttatttacg taattaaatg ttcttattct
     4081 gtgcatggta attcactcaa tcctctattc agagccgcct atccagctgc tttttacact
     4141 ttacatgttt attttgatag gtttgaaaca cagggaaggt ttcatctgta tggttatcga
     4201 aatactggag aatcagccag acaaggatga cgaagggcga attctgcaga tatccatcac
     4261 actggcggcc gctcgagcat gcatctagag ggcccaattc gccctatagt gagtcgtatt
     4321 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4381 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4441 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
     4501 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4561 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4621 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4681 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4741 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4801 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4861 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4921 aattcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa
     4981 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
     5041 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
     5101 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
     5161 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
     5221 agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     5281 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5341 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5401 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     5461 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5521 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc ccaccttgct
     5581 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5641 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5701 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5761 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     5821 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5881 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5941 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6001 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattgaaaaa
     6061 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6121 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6181 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6241 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6301 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6361 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6421 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6481 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6541 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6601 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6661 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6721 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6781 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6841 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6901 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6961 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7021 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7081 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7141 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7201 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7261 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7321 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7381 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7441 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7501 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7561 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7621 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7681 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7741 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7801 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7861 aagcggaag
//
