LOCUS       62056_13190        8121 bp DNA     circular SYN 02-NOV-2020
DEFINITION  Cloning vector pJL04, complete sequence.
ACCESSION   MT370811
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     A set of shuttle plasmids for gene expression in Acinetobacter 
            baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8121)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2020) First Hospital, Jilin University, Xi'an 
            Road, Changchun, Jilin 130062, China
REFERENCE   3  (bases 1 to 8121)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2020) First Hospital, Jilin University, Xi'an Road, 
            Changchun, Jilin 130062, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(530..1060)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1249..1277)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(1295..1399)
                     /label=AmpR promoter
     terminator      complement(1435..1462)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(1466..2545)
                     /label=lacI
                     /note="lac repressor"
     regulatory      complement(2546..2745)
                     /label=derived from pRpoD
                     /note="derived from pRpoD"
                     /regulatory_class="promoter"
     protein_bind    2752..2768
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2938..2961
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     rep_origin      complement(3097..3685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      5084..6419
                     /label=oriV Acb
                     /note="oriV Acb"
                     /regulatory_class="replication_regulatory_region"
ORIGIN
        1 cccctcatgt gcatggggga agtgatgaaa agaaacccat cacgcccaca tactaatgac
       61 aacaagaagc cttacaccgc aagggctagg gaagaaagtc agggagctag atcagaactc
      121 aacgctgaaa aaaatcaggg aaaaagtggc ggatctgacg aacgtccacc tagagcgagc
      181 agggcttgag gtgagagttg atcaccgcag tcataaggat cgtggactag acatcgagcc
      241 gacattcaag gagggacttg aagccaccca cgccaagcgc aggggggagg aactggagat
      301 cgtcaagcgt aatgatgaaa tcaaaaagcg caataaaaaa gctgaaaggc ttgatatgtc
      361 tatcattgag acgagcaaca gagtagagca attgacactt gaacagcagc aacagcagca
      421 ggggctgcat acttggtctg acaatcgatg cgaattggcc gcggcgttgt gacaatttac
      481 cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
      541 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
      601 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
      661 cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
      721 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
      781 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
      841 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
      901 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
      961 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
     1021 gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc
     1081 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
     1141 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     1201 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
     1261 gaacaggctt atgtcaattc ggttgaatac tcatactctt cctttttcaa tattattgaa
     1321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc cgagaaaagg
     1441 ccatccgtca ggatggcctt cttcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1501 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg
     1561 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct
     1621 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
     1681 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg
     1741 aaatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca
     1801 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg
     1861 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     1921 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
     1981 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
     2041 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
     2101 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
     2161 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
     2221 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
     2281 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
     2341 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg
     2401 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt
     2461 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga
     2521 catcgtataa cgttactggt ttcatctttg cttactcttt gttgaatctg tggcagtgaa
     2581 aaaccgtgca tgatagcaca atttgcttga aatgtttggg ctttgatcgg tgaccgttaa
     2641 ataaaaaata tctttgaatt cacgactatt ttttgaatat tgggctaaaa tcattttttt
     2701 caaggttaaa tcatagaaaa atttgtagaa atcttcgctt atcaattgac attgtgagcg
     2761 gataacaata taatttggaa ttgtgagcgg ataacaattt cacattggaa ttgtgagcgg
     2821 ataacaattt cacttggaat tgtgagcgga taacaattta aattggaatt gtgagcggat
     2881 aacaatttca caaattggaa ttgtgagcgg ataacaattt tccgaggact gcccatggac
     2941 tacaaagacg atgacgacaa gggatcccac cgcggtgtct agaactagtc ccgggctgca
     3001 ggaattcgat atcaagctta tcgataccgt cgacggtacc cgcggccgag gcctgcaaaa
     3061 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3121 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3181 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3241 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3301 ctgtaggtat ctctgttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3361 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3421 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3481 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3541 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3601 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3661 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3721 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3781 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3841 aacttgtgat cccccctgga ggatgccgta gaccaagccc ccgcagtcta tgccaccgca
     3901 caacaggcac tatacgacca ccaacaacac cgccaaacgc ttatagatgt gtatttgatt
     3961 gaagtaaaaa cggatgagaa gcgagcagag catagatttt acaaggcttt gcaagacaat
     4021 atcgaccgct tgcagcttga taaagtgatc aaagagggac gagcagctac agcactgcta
     4081 agggagatcg gagagccact gccgccacca gtgaaagtga aaacaggttg gtttagtgct
     4141 gattattacg gctttgagga catgctcaca cgttgggata atgatcaatc tagcctttcc
     4201 atttatcaca gagagataaa agaggagcaa caggaacagc aacgaaaaca acagcagata
     4261 gaaagagaaa ctaaacaaaa acaggaagat gacaaaaaga aacggcagga ctatgaaaga
     4321 tatttatcaa aatacggcta tgtcaaagat gctgagtatg accatatcag cgaccatgtt
     4381 gcttcagaca tttccatgtt tacacgtctt caggcgcagg catgggcaag taacgatatg
     4441 caagagtaca taaggtgttc aaagcaaaaa tacgagcaga tcagcgacag aatagcctat
     4501 gagcgtgact tgaaaaaaat cgggcaattg agccgtatcc tggataaaga cagacaggca
     4561 cttgctgata ttttaccgca gttgatgcct tactatgagc agatgcaaca ggctgttaat
     4621 aaacggaaaa ttttacttga gaatgagcga cagccatcat ttaaaccacg ttatgaccaa
     4681 gaacagccta agccgaaaaa agacaatgac ctaacttttt aatgtgttac aatagcattc
     4741 aaatgtaaca cattggagtg aaaaacatga cagaagcgac ttttaccttt cgggtagacc
     4801 atgacctaaa gcaagaattt tcaagccttg ctaagactgt tgatcggtcg ggggcgcagc
     4861 taatccgtga cttcatgcgt gactttgtta agaagcaaca ggaagccgca gactatgaca
     4921 aatggttcaa gcagcaggta cagatcggct taaatgaagc caatgcaggc aagttgattc
     4981 cacatgagga cgtaaaggca gagtttgccg ctagacgtgc tgcaacattg gcgaaactgg
     5041 cagcgaagaa tgaagattga gtggactgaa acagctcgcc aagatcgtag aaatatctat
     5101 gattatcttg aagaacgcaa ccctatagca gctattgaaa ttgatgattt aattgaagaa
     5161 aagacagatt tacttgttga taatcgactg atggggcgca caggcagaca gaaagatact
     5221 agggagttag tgatacatcc gcattatgtg gttgtatatg acatcactga tataatacgg
     5281 atactcagag tgctacacac atcgcaggag tggtcatgac ttactcatgt actttggatt
     5341 atttagtgtt ataaaatcct gatttataaa ttttttttgt taaaaaagat aaaagcccct
     5401 tgcaattgct tggggcttta ccgtaattta tggggtacag atcttcgata ctgacatatc
     5461 ggcaatcgaa agcattaagg tttgacgacc gctaatgatt tcaccacagg ggcttaatgt
     5521 acctgtctta aattctaagg ttttaactcg ctttgtcaag catagacccc aaaaatttag
     5581 ccaatgtctg taactcaatc tgtccatgtg tgggtgatga ggtacagtga cgctagcaca
     5641 catcggaaaa acgctattac taggggaact gaacagagta gcggacgcaa tgagtagtca
     5701 tttaattggc ggttatgagc gtgttcaggc ggtgctatca atcgtaatca taacagtggc
     5761 agcttgatac agtgatgtca tccctgatgc gaaagcgacc gaccgacggt acatcgaatg
     5821 ggaatacttt agggtgattt ttaagaatcg ctctagggtg agtatttccc attcagctct
     5881 gctccctccc tctggtactt taatcaaaag cactactaaa catatgtttt taaataaaaa
     5941 atattgatat agagataata ttagtaagaa taattaaaca attgaatata gataaatcat
     6001 tgttaaataa agattaatta ttaaaatgaa tgtatactta tatataaatc aatgatttaa
     6061 aatatttgat aaagaaaact tttcaaaaaa aatataattg agattgtgtc atttcggtca
     6121 attcttaata tgttccacgc aagttttagc tatggtgcta aacagaaatt tgctgaaaaa
     6181 gaacttttca ctgaactggt taaaatgtaa gcagcctgag agccgccaaa aattttaaaa
     6241 acaaaccgcc ttaatcatct tcaaaaaata cctctaaaac ctcaccattt gcgttttaag
     6301 acccatattt catcctgccc ttatgttccc atgctgatag ctataaagtg tctgtaatcg
     6361 cttcctatga cgttctaggc tgttgataac ttttggaaca acgcaaaatg ttaaaatccg
     6421 atcatttttt aacctagtta ttttcgttac agggtaactt tggtagtatt atttcaatat
     6481 ttagttgtta caggataaca aaatatatgt tacagggtaa ttaatttagc gttacaggat
     6541 aactatagtt acagggtaac gttaaatagt tatcctgtaa tttaaaaata gttgcaggat
     6601 agcataaatt tttacaaata aattttataa aaagttatcc tgtaactaaa aacaatgtta
     6661 tcctgtaact atgatatact atatgggagt taaagacatg aaagacccga acgatcagaa
     6721 aacacaggac atgctaaaag agccgccaaa aaccaatgcc gaacgacaaa aagcatatcg
     6781 tgaaaaacgc aaaagccttg atagtcaacg tttggaggtg tttatagata agggtgtgtc
     6841 ggatatgctt gcggacatgg tgggcgcagc aggggagagc caaaaagcca ttttgaccgc
     6901 attgattgag aaagagtata agcggctgta tgcagtgaaa aaatagcaag ctaaaaaagt
     6961 acattgattc aaaaagtaat gcagataaaa aagaaaccct cgatttatga gggtttttta
     7021 taactactaa tgcctatgaa aataagtgct tatcattttc tctaatgtac ccaataaaat
     7081 cggggttttg ttccacaagt ttcttgatgt cattatcatg tttcttcagt aagtaagccc
     7141 cgatcaggat cttgcgtttt gcatcgtctg accttgcctg tttttgctct cgacctttca
     7201 ccacagcgtc agctagatcc tttcttgcct tttcctgacg ttccaatttt tccttttctt
     7261 catcgagcat ttgacccaat tccatagctg tttttttgac catttttaat gcctgtgtct
     7321 caacctattt tccgaagtat gaaccatcat caatgtgaaa agtgttctac ttcaaaaact
     7381 cgacttacat caagatccga gcgcagcgag caaaatcaaa aaacaaagtc aaagccttat
     7441 tgctcttgct tttgctttta gcctcgcaga gttcccgaag ggcgcactta cgcaaaattt
     7501 ttgctacgcc aaattttgca agtacggtca gggaaacccc gacaccccaa ccgcccaaaa
     7561 cttggggcgg ctgttaataa acaggtgatg aaaatggcaa tttaccattg tgaaatgcag
     7621 aacatttcga ggtcagatgg tcgctcaatc gtggcatgtg cagcataccg agcaggcgaa
     7681 aaattgtact gtgatacgta cggaaaagag caggactaca ccaaaaaaac aggcattgaa
     7741 tacacccaaa tttttgcccc aactggggca agtcctgaca tgttagatcg tcaaacccta
     7801 tggaatcggg tagagcaatc cgaactaaaa aagaacggtg acatcaaaca ggaggcaaga
     7861 ttagcaaagg aagtagagat agcattgccg catgaactgg ataagacaca gcgtcaggca
     7921 cttgttaccg agttgtgcca gtccttagtt aaagcctatg gagtggcggt ggacgtagcg
     7981 atccatgccc ctcatgtgca tgggggaagg agaaagaaac ctcacgccca cataagacac
     8041 aggcgtcagg caacttgtta accgagttgt gccagtcctt agttaaagcc tatggagtgg
     8101 cggtggacgt agcgatccat g
//
