LOCUS       62056_13175        9519 bp DNA     circular SYN 02-NOV-2020
DEFINITION  Cloning vector pJL01, complete sequence.
ACCESSION   MT370813
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     A set of shuttle plasmids for gene expression in Acinetobacter 
            baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2020) First Hospital, Jilin University, Xi'an 
            Road, Changchun, Jilin 130062, China
REFERENCE   3  (bases 1 to 9519)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2020) First Hospital, Jilin University, Xi'an Road, 
            Changchun, Jilin 130062, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..973
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             1487..2323
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             2313..3161
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(3533..4408)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4435..4719
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             4757..4780
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     primer_bind     complement(4832..4848)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5833..6645
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(7462..7856)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            8198..8285
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
ORIGIN
        1 cccgatgaag aacgacagga ctttgcaggc cataggccga cagctcaagg ccatgggctg
       61 tgagcgcttc gatatcggcg tcagggacgc caccaccggc cagatgatga accgggaatg
      121 gtcagccgcc gaagtgctcc agaacacgcc atggctcaag cggatgaatg cccagggcaa
      181 tgacgtgtat atcaggcccg ccgagcagga gcggcatggt ctggtgctgg tggacgacct
      241 cagcgagttt gacctggatg acatgaaagc cgagggccgg gagcctgccc tggtagtgga
      301 aaccagcccg aagaactatc aggcatgggt caaggtggcc gacgccgcag gcggtgaact
      361 tcgggggcag attgcccgga cgctggccag cgagtacgac gccgacccgg ccagcgccga
      421 cagccgccac tatggccgct tggcgggctt caccaaccgc aaggacaagc acaccacccg
      481 cgccggttat cagccgtggg tgctgctgcg tgaatccaag ggcaagaccg ccaccgctgg
      541 cccggcgctg gtgcagcagg ctggccagca gatcgagcag gcccagcggc agcaggagaa
      601 ggcccgcagg ctggccagcc tcgaactgcc cgagcggcag cttagccgcc accggcgcac
      661 ggcgctggac gagtaccgca gcgagatggc cgggctggtc aagcgcttcg gtgatgacct
      721 cagcaagtgc gactttatcg ccgcgcagaa gctggccagc cggggccgca gtgccgagga
      781 aatcggcaag gccatggccg aggccagccc agcgctggca gagcgcaagc ccggccacga
      841 agcggattac atcgagcgca ccgtcagcaa ggtcatgggt ctgcccagcg tccagcttgc
      901 gcgggccgag ctggcacggg caccggcacc ccgccagcga ggcatggaca ggggcgggcc
      961 agatttcagc atgtagtgct tgcgttggta ctcacgcctg ttatactatg agtactcacg
     1021 cacagaaggg ggttttatgg aatacgaaaa aagcgcttca gggtcggtct acctgatcaa
     1081 aagtgacaag ggctattggt tgcccggtgg ctttggttat acgtcaaaca aggccgaggc
     1141 tggccgcttt tcagtcgctg atatggccag ccttaacctt gacggctgca ccttgtcctt
     1201 gttccgcgaa gacaagcctt tcggccccgg caagtttctc ggtgactgat atgaaagacc
     1261 aaaaggacaa gcagaccggc gacctgctgg ccagccctga cgctgtacgc caagcgcgat
     1321 atgccgagcg catgaaggcc aaagggatgc gtcagcgcaa gttctggctg accgacgacg
     1381 aatacgaggc gctgcgcgag tgcctggaag aactcagagc ggcgcagggc gggggtagtg
     1441 accccgccag cgcctaacca ccaactgcct gcaaaggagg caatcaatgg ctacccataa
     1501 gcctatcaat attctggagg cgttcgcagc agcgccgcca ccgctggact acgttttgcc
     1561 caacatggtg gccggtacgg tcggggcgct ggtgtcgccc ggtggtgccg gtaaatccat
     1621 gctggccctg caactggccg cacagattgc aggcgggccg gatctgctgg aggtgggcga
     1681 actgcccacc ggcccggtga tctacctgcc cgccgaagac ccgcccaccg ccattcatca
     1741 ccgcctgcac gcccttgggg cgcacctcag cgccgaggaa cggcaagccg tggctgacgg
     1801 cctgctgatc cagccgctga tcggcagcct gcccaacatc atggccccgg agtggttcga
     1861 cggcctcaag cgcgccgccg agggccgccg cctgatggtg ctggacacgc tgcgccggtt
     1921 ccacatcgag gaagaaaacg ccagcggccc catggcccag gtcatcggtc gcatggaggc
     1981 catcgccgcc gataccgggt gctctatcgt gttcctgcac catgccagca agggcgcggc
     2041 catgatgggc gcaggcgacc agcagcaggc cagccggggc agctcggtac tggtcgataa
     2101 catccgctgg cagtcctacc tgtcgagcat gaccagcgcc gaggccgagg aatggggtgt
     2161 ggacgacgac cagcgccggt tcttcgtccg cttcggtgtg agcaaggcca actatggcgc
     2221 accgttcgct gatcggtggt tcaggcggca tgacggcggg gtgctcaagc ccgccgtgct
     2281 ggagaggcag cgcaagagca agggggtgcc ccgtggtgaa gcctaagaac aagcacagcc
     2341 tcagccacgt ccggcacgac ccggcgcact gtctggcccc cggcctgttc cgtgccctca
     2401 agcggggcga gcgcaagcgc agcaagctgg acgtgacgta tgactacggc gacggcaagc
     2461 ggatcgagtt cagcggcccg gagccgctgg gcgctgatga tctgcgcatc ctgcaagggc
     2521 tggtggccat ggctgggcct aatggcctag tgcttggccc ggaacccaag accgaaggcg
     2581 gacggcagct ccggctgttc ctggaaccca agtgggaggc cgtcaccgct gatgccatgg
     2641 tggtcaaagg tagctatcgg gcgctggcaa aggaaatcgg ggcagaggtc gatagtggtg
     2701 gggcgctcaa gcacatacag gactgcatcg agcgcctttg gaaggtatcc atcatcgccc
     2761 agaatggccg caagcggcag gggtttcggc tgctgtcgga gtacgccagc gacgaggcgg
     2821 acgggcgcct gtacgtggcc ctgaacccct tgatcgcgca ggccgtcatg ggtggcggcc
     2881 agcatgtgcg catcagcatg gacgaggtgc gggcgctgga cagcgaaacc gcccgcctgc
     2941 tgcaccagcg gctgtgtggc tggatcgacc ccggcaaaac cggcaaggct tccatagata
     3001 ccttgtgcgg ctatgtctgg ccgtcagagg ccagtggttc gaccatgcgc aagcgccgcc
     3061 agcgggtgcg cgaggcgttg ccggagctgg tcgcgctggg ctggacggta accgagttcg
     3121 cggcgggcaa gtacgacatc acccggccca aggcggcagg ctgacccccc ccactctatt
     3181 gtaaacaaga catttttatc ttttatattc aatggcttat tttcctgcta attggtaata
     3241 ccatgaaaaa taccatgctc agaaaaggct taacaatatt ttgaaaaatt gcctactgag
     3301 cgctgccgca cagctccata ggccgctttc ctggctttgc ttccagatgt atgctcttct
     3361 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3421 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3481 catgtctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact
     3541 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
     3601 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
     3661 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
     3721 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
     3781 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
     3841 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
     3901 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
     3961 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
     4021 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
     4081 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
     4141 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
     4201 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
     4261 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
     4321 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
     4381 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
     4441 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
     4501 taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
     4561 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
     4621 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatccta
     4681 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagca
     4741 ggaggaattc accatggact acaaagacga tgacgacaag agatctctgc aggtcgactc
     4801 tagaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact
     4861 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     4921 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     4981 gcgaatgtct ggtcggtgaa gtgggctttg gcgccgccaa tatgttctac gatccggctg
     5041 acagagacga cctttgtctc gatcctagac gcattgcaca gatggcggac gcattctctc
     5101 gtgcgctgga cgtcgatccg cgtcgcctgc tcgaccaggc gtacgcttat gggtgccttt
     5161 ccgcagcttg gaacgcggat ggagaagagg agcaacgcga tctagctatc gcggccgcga
     5221 tcaagcaggt gcgacagacg tcatactaga tatcaagcga cttctcctat cccctgggaa
     5281 cacatcaatc tcaccggaga atatcgctgg ccaaagcctt agcgtaggat tccgcccctt
     5341 cccgcaaacg accccaaaca ggaaacgcag ctgtaatccg ggcagcgcaa cggaacattc
     5401 atcagtgtaa aaatggaatc aataaagccc tgcgcagcgc gcagggtcag cctgaatacg
     5461 cgtttaatga ccagcacagt cgtgatggca aggtcagaat agcgctgagg tctgcctcgt
     5521 gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg
     5581 agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt
     5641 tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca
     5701 gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt
     5761 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     5821 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat
     5881 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     5941 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     6001 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     6061 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     6121 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca
     6181 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     6241 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     6301 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     6361 caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat
     6421 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     6481 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     6541 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     6601 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     6661 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa
     6721 taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc
     6781 gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca
     6841 tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc tggtatgagt
     6901 cagcaacacc ttcttcacga ggcagacctc agcgctattc tgaccttgcc atcacgactg
     6961 tgctggtcat taaacgcgta ttcaggctga ccctgcgcgc tgcgcagggc tttattgatt
     7021 ccatttttac actgatgaat gttccgttgc gctgcccgga ttacagctga aagcgaccag
     7081 gtgctcggcg tggcaagact cgcagcgaac ccgtagaaag ccatgctcca gccgcccgca
     7141 ttggagaaat tcttcaaatt cccgttgcac atagcccggc aattcctttc cctgctctgc
     7201 cataagcgca gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa gggtttgctc
     7261 gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc tggccgccac
     7321 atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat acagtctatc gcttagcgga
     7381 aagttctttt accctcagcc gaaatgcctg ccgttgctag acattgccag ccagtgcccg
     7441 tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc ctgcaataac
     7501 tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag caggggcggg
     7561 ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc actaggcgcg
     7621 gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc cagaggccgg
     7681 atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc ggcaggggga
     7741 aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc tggagcgctt
     7801 ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt gcagccccgc
     7861 agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc ggcagaccga
     7921 acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg agccgatcct
     7981 ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc accttgcgcc
     8041 ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg cacccgctga atttcggcat
     8101 tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg ttgctcccct
     8161 taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg gaggcacagc
     8221 ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc tcttcgagaa
     8281 actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc atggagccga
     8341 aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga aaaccggcag
     8401 caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg aaggcaagta
     8461 tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg agttcgtcga
     8521 gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg ggcggctgtt
     8581 caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga aggcgctggc
     8641 gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc tggccatcca
     8701 tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga tcaatgacgg
     8761 catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc cggagaaggg
     8821 cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga cccgcgaggc
     8881 atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc gcattgacca
     8941 cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg ggccgaacgt
     9001 ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc tgaacatcga
     9061 caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag accatgaacg
     9121 caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag atcgaaccgc
     9181 tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc cagacccagc
     9241 aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca ggggcggcat
     9301 gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc gccgcgcaga
     9361 gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg atgctggctt
     9421 ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac ctgacgccac
     9481 tgacaaccga ggacggctcg atctggctgc gcttggtgg
//
