LOCUS       V006590                11329 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V006590
VERSION     V006590
KEYWORDS    pJL-TRBO-G
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Lindbo JA
  TITLE     TRBO: a high-efficiency tobacco mosaic virus RNA-based
            overexpression vector.
  JOURNAL   Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24.
   PUBMED   17720752
REFERENCE   2  (bases 1 to 11329)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant
            Physiol."; date: "2007-12"; volume: "145(4)"; pages: "1232-40. Epub
            2007 Aug 24"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..46
                     /label="minimal CaMV 35S promoter"
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    58..113
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             4949..5752
                     /gene="MP"
                     /label="Movement protein"
                     /note="Movement protein from Tobacco mosaic virus (strain
                     vulgare). Accession#: P03583"
     CDS             5811..6527
                     /label="Cycle 3 GFP"
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
     misc_feature    7137..7161
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(7319..8464)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(8766..9557)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(9766..10401)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    10481..10505
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     10586..10602
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagggtat ttttacaaca
       61 attaccaaca acaacaaaca acagacaaca ttacaattac tatttacaat tacaatggca
      121 tacacacaga cagctaccac atcagctttg ctggacactg tccgaggaaa caactccttg
      181 gtcaatgatc tagcaaagcg tcgtctttac gacacagcgg ttgaagagtt taacgctcgt
      241 gaccgcaggc ccaaggtgaa cttttcaaaa gtaataagcg aggagcagac gcttattgct
      301 acccgggcgt atccagaatt ccaaattaca ttttataaca cgcaaaatgc cgtgcattcg
      361 cttgcaggtg gattgcgatc tttagaactg gaatatctga tgatgcaaat tccctacgga
      421 tcattgactt atgacatagg cgggaatttt gcatcgcatc tgttcaaggg acgagcatat
      481 gtacactgct gcatgcccaa cctggacgtt cgagacatca tgcggcacga aggccagaaa
      541 gacagtattg aactatacct ttctaggcta gagagagggg ggaaaacagt ccccaacttc
      601 caaaaggaag catttgacag atacgcagaa attcctgaag acgctgtctg tcacaatact
      661 ttccagacat gcgaacatca gccgatgcag caatcaggca gagtgtatgc cattgcgcta
      721 cacagcatat atgacatacc agccgatgag ttcggggcgg cactcttgag gaaaaatgtc
      781 catacgtgct atgccgcttt ccacttctcc gagaacctgc ttcttgaaga ttcatgcgtc
      841 aatttggacg aaatcaacgc gtgtttttcg cgcgatggag acaagttgac cttttctttt
      901 gcatcagaga gtactcttaa ttactgtcat agttattcta atattcttaa gtatgtgtgc
      961 aaaacttact tcccggcctc taatagagag gtttacatga aggagttttt agtcaccaga
     1021 gttaatacct ggttttgtaa gttttctaga atagatactt ttcttttgta caaaggtgtg
     1081 gcccataaaa gtgtagatag tgagcagttt tatactgcaa tggaagacgc atggcattac
     1141 aaaaagactc ttgcaatgtg caacagcgag agaatcctcc ttgaggattc atcatcagtc
     1201 aattactggt ttcccaaaat gagggatatg gtcatcgtac cattattcga catttctttg
     1261 gagactagta agaggacgcg caaggaagtc ttagtgtcca aggatttcgt gttcacagtg
     1321 cttaaccaca ttcgaacata ccaggcgaaa gctcttacat acgcaaatgt tttgtccttc
     1381 gtcgaatcga ttcgatcgag ggtaatcatt aacggtgtga cagcgaggtc cgaatgggat
     1441 gtggacaaat ctttgttaca atccttgtcc atgacgtttt acctgcatac taagcttgcc
     1501 gttctaaagg atgacttact gattagcaag tttagtctcg gttcgaaaac ggtgtgccag
     1561 catgtgtggg atgagatttc gctggcgttt gggaacgcat ttccctccgt gaaagagagg
     1621 ctcttgaaca ggaaacttat cagagtggca ggcgacgcat tagagatcag ggtgcctgat
     1681 ctatatgtga ccttccacga cagattagtg actgagtaca aggcctctgt ggacatgcct
     1741 gcgcttgaca ttaggaagaa gatggaagaa acggaagtga tgtacaatgc actttcagaa
     1801 ttatcggtgt taagggagtc tgacaaattc gatgttgatg ttttttccca gatgtgccaa
     1861 tctttggaag ttgacccaat gacggcagcg aaggttatag tcgcggtcat gagcaatgag
     1921 agcggtctga ctctcacatt tgaacgacct actgaggcga atgttgcgct agctttacag
     1981 gatcaagaga aggcttcaga aggtgcattg gtagttacct caagagaagt tgaagaaccg
     2041 tccatgaagg gttcgatggc cagaggagag ttacaattag ctggtcttgc tggagatcat
     2101 ccggaatcgt cctattctaa gaacgaggag atagagtctt tagagcagtt tcatatggcg
     2161 acggcagatt cgttaattcg taagcagatg agctcgattg tgtacacggg tccgattaaa
     2221 gttcagcaaa tgaaaaactt tatcgatagc ctggtagcat cactatctgc tgcggtgtcg
     2281 aatctcgtca agatcctcaa agatacagct gctattgacc ttgaaacccg tcaaaagttt
     2341 ggagtcttgg atgttgcatc taggaagtgg ttaatcaaac caacggccaa gagtcatgca
     2401 tggggtgttg ttgaaaccca cgcgaggaag tatcatgtgg cgcttttgga atatgatgag
     2461 cagggtgtgg tgacatgcga tgattggaga agagtagctg ttagctctga gtctgttgtt
     2521 tattccgaca tggcgaaact cagaactctg cgcagactgc ttcgaaacgg agaaccgcat
     2581 gtcagtagcg caaaggttgt tcttgtggac ggagttccgg gctgtggaaa aaccaaagaa
     2641 attctttcca gggttaattt tgatgaagat ctaattttag tacctgggaa gcaagccgcg
     2701 gaaatgatca gaagacgtgc gaattcctca gggattattg tggccacgaa ggacaacgtt
     2761 aaaaccgttg attctttcat gatgaatttt gggaaaagca cacgctgtca gttcaagagg
     2821 ttattcattg atgaagggtt gatgttgcat actggttgtg ttaattttct tgtggcgatg
     2881 tcattgtgcg aaattgcata tgtttacgga gacacacagc agattccata catcaataga
     2941 gtttcaggat tcccgtaccc cgcccatttt gccaaattgg aagttgacga ggtggagaca
     3001 cgcagaacta ctctccgttg tccagccgat gtcacacatt atctgaacag gagatatgag
     3061 ggctttgtca tgagcacttc ttcggttaaa aagtctgttt cgcaggagat ggtcggcgga
     3121 gccgccgtga tcaatccgat ctcaaaaccc ttgcatggca agatcctgac ttttacccaa
     3181 tcggataaag aagctctgct ttcaagaggg tattcagatg ttcacactgt gcatgaagtg
     3241 caaggcgaga catactctga tgtttcacta gttaggttaa cccctacacc ggtctccatc
     3301 attgcaggag acagcccaca tgttttggtc gcattgtcaa ggcacacctg ttcgctcaag
     3361 tactacactg ttgttatgga tcctttagtt agtatcatta gagatctaga gaaacttagc
     3421 tcgtacttgt tagatatgta taaggtcgat gcaggaacac aatagcaatt acagattgac
     3481 tcggtgttca aaggttccaa tctttttgtt gcagcgccaa agactggtga tatttctgat
     3541 atgcagtttt actatgataa gtgtctccca ggcaacagca ccatgatgaa taattttgat
     3601 gctgttacca tgaggttgac tgacatttca ttgaatgtca aagattgcat attggatatg
     3661 tctaagtctg ttgctgcgcc taaggatcaa atcaaaccac taatacctat ggtacgaacg
     3721 gcggcagaaa tgccacgcca gactggacta ttggaaaatt tagtggcgat gattaaaaga
     3781 aactttaacg cacccgagtt gtctggcatc attgatattg aaaatactgc atctttggtt
     3841 gtagataagt tttttgatag ttatttgctt aaagaaaaaa gaaaaccaaa taaaaatgtt
     3901 tctttgttca gtagagagtc tctcaataga tggttagaaa agcaggaaca ggtaacaata
     3961 ggccagctcg cagattttga ttttgtggat ttgccagcag ttgatcagta cagacacatg
     4021 attaaagcac aacccaaaca aaagttggac acttcaatcc aaacggagta cccggctttg
     4081 cagacgattg tgtaccattc aaaaaagatc aatgcaatat tcggcccgtt gtttagtgag
     4141 cttaccaggc aattactgga cagtgttgat tcgagcagat ttttgttttt cacaagaaag
     4201 acaccagcgc agattgagga tttcttcgga gatctcgaca gtcatgtgcc gatggatgtc
     4261 ttggagctgg atatatcaaa atacgacaaa tctcagaatg aattccactg tgcagtagaa
     4321 tacgagatct ggcgaagatt gggtttcgaa gacttcttgg gagaagtttg gaaacaaggg
     4381 catagaaaga ccaccctcaa ggattatacc gcaggtataa aaacttgcat ctggtatcaa
     4441 agaaagagcg gggacgtcac gacgttcatt ggaaacactg tgatcattgc tgcatgtttg
     4501 gcctcgatgc ttccgatgga gaaaataatc aaaggagcct tttgcggtga cgatagtctg
     4561 ctgtactttc caaagggttg tgagtttccg gatgtgcaac actccgcgaa tcttatgtgg
     4621 aattttgaag caaaactgtt taaaaaacag tatggatact tttgcggaag atatgtaata
     4681 catcacgaca gaggatgcat tgtgtattac gatcccctaa agttgatctc gaaacttggt
     4741 gctaaacaca tcaaggattg ggaacacttg gaggagttca gaaggtctct ttgtgatgtt
     4801 gctgtttcgt tgaacaattg tgcgtattac acacagttgg acgacgctgt atgggaggtt
     4861 cataagaccg cccctccagg ttcgtttgtt tataaaagtc tggtgaagta tttgtctgat
     4921 aaagttcttt ttagaagttt gtttatagat ggctctagtt gttaaaggaa aagtgaatat
     4981 caatgagttt atcgacctga caaaaatgga gaagatctta ccgtcgatgt ttacccctgt
     5041 aaagagtgtt atgtgttcca aagttgataa aataatggtt catgagaatg agtcattgtc
     5101 aggggtgaac cttcttaaag gagttaagct tattgatagt ggatacgtct gtttagccgg
     5161 tttggtcgtc acgggcgagt ggaacttgcc tgacaattgc agaggaggtg tgagcgtgtg
     5221 tctggtggac aaaaggatgg aaagagccga cgaggccatt ctcggatctt actacacagc
     5281 agctgcaaag aaaagatttc agttcaaggt cgttcccaat tatgctataa ccacccagga
     5341 cgcgatgaaa aacgtctggc aagttttagt taatattaga aatgtgaaga tgtcagcggg
     5401 tttctgtccg ctttctctgg agtttgtgtc ggtgtgtatt gtttatagaa ataatataaa
     5461 attaggtttg agagagaaga ttacaaacgt gagagacgga gggcccatgg aacttacaga
     5521 agaagtcgtt gatgagttca tggaagatgt ccctatgtcg atcaggcttg caaagtttcg
     5581 atctcgaacc ggaaaaaaga gtgatgtccg caaagggaaa aatagtagta gtgatcggtc
     5641 agtgccgaac aagaactata gaaatgttaa ggattttggg ggaatgagtt ttaaaaagaa
     5701 taatttaatc gatgatgatt cggaggctac tgtcgccgaa tcggattcgt tttaaataga
     5761 tcttacagta tcactactcc atctcagttc gtgttcttgt cattaattaa atggctagca
     5821 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     5881 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgctacatac ggaaagctta
     5941 cacttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
     6001 ctttctctta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa cggcatgact
     6061 ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct ttcaaagatg
     6121 acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta
     6181 tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac aaactcgagt
     6241 acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag
     6301 ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca gaccattatc
     6361 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga
     6421 cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt
     6481 ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaataa actagggcgg
     6541 ccgcggtcct gcaacttgag gtagtcaaga tgcataataa ataacggatt gtgtccgtaa
     6601 tcacacgtgg tgcgtacgat aacgcatagt gtttttccct ccacttaaat cgaagggttg
     6661 tgtcttggat cgcgcgggtc aaatgtatat ggttcatata catccgcagg cacgtaataa
     6721 agcgaggggt tcgaatcccc ccgttacccc cggtaggggc ccaggtaccc ggatgtgttt
     6781 tccgggctga tgagtccgtg aggacgaaac ctggctgcag gcatgcaagc ttggcgtaat
     6841 catggtcata gcctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct
     6901 ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata
     6961 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
     7021 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgacct gcagcccggc
     7081 cgggggatcc actagcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
     7141 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     7201 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacaggag
     7261 atctcagtaa agcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc
     7321 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca
     7381 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc
     7441 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat
     7501 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg
     7561 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg
     7621 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt
     7681 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct
     7741 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga
     7801 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc
     7861 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct
     7921 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg
     7981 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg
     8041 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca
     8101 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct
     8161 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct
     8221 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag
     8281 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctgggccatc gagccgacgg
     8341 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct
     8401 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat
     8461 tcattcaccc tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct
     8521 gatttgaccc gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg
     8581 tcccgtagac cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga
     8641 ataccagcga ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact
     8701 tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cacatctagg
     8761 tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc
     8821 cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac
     8881 cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata
     8941 aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa
     9001 aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct
     9061 ttaaatgggg tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt
     9121 aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg
     9181 tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt
     9241 tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg
     9301 ctccagccat catgccgttc aaggtgcagg acctttggaa caggcagctt tccttccagc
     9361 catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc
     9421 gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac
     9481 attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat
     9541 attctcattt tagccattta ttatttcctt cctcttttct acagtattta aagatacccc
     9601 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacttta
     9661 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
     9721 cggagccgat tttgaaacca caattatggg tgatgctgca actcgagcgg gccgggaggg
     9781 ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct
     9841 ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt
     9901 ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct
     9961 caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc
    10021 gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc
    10081 ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt
    10141 gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg
    10201 ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg
    10261 ccaagttttc cgcgaggtat ccacaacgcc ggcggccggc cgcggtgtct cgcacacggc
    10321 ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg
    10381 gcaaccagcc cggtgagcgt ctctagtgga ctgatgggct gcctgtatcg agtggtgatt
    10441 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
    10501 taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta aagtactggg
    10561 gtggttttgg taccgggccc cccctcgagg tcgacggtat cgataagctt gatatcgaat
    10621 tcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
    10681 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
    10741 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
    10801 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
    10861 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
    10921 ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag cacgacacac
    10981 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt
    11041 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact
    11101 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
    11161 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
    11221 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
    11281 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttc
//
