LOCUS       V005322                 4824 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005322
VERSION     V005322
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Gueldener U, Heinisch J, Koehler GJ, Voss D, Hegemann JH.
  TITLE     A second set of loxP marker cassettes for Cre-mediated multiple gene
            knockouts in budding yeast
  JOURNAL   Nucleic Acids Res. 30 (6), E23 (2002)
   PUBMED   11884642
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Gueldener U, Heinisch JH, Voss D, Koehler G, Hegemann JH.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2000) Institut fuer Mikrobiologie,
            Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf
            40225, Germany
REFERENCE   3  (bases 1 to 4824)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4824)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2002"; volume: "30"; issue: "6"; pages: "E23"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-AUG-2000) Institut fuer Mikrobiologie,
            Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf
            40225, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    53..86
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(243..1328)
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Kluyveromyces
                     lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267
                     / NRRL Y-1140 / WM37). Accession#: P23390"
     misc_feature    2359..2412
                     /label="loxP site"
                     /note="loxP site"
     protein_bind    complement(2375..2408)
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     promoter        complement(2462..2480)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2738..3326)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3500..4357)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4358..4462)
                     /label="AmpR promoter"
     promoter        4808..4824
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acaaccctta atataacttc
       61 gtataatgta tgctatacga agttattagg tctagagatc cgcaggctaa ccggaacctg
      121 tattatttag tttatgctac gttaaataaa gacctttcgt tcacataact gaatgtgtaa
      181 tggccttgag atttcaagca taccaagttg gtggagacgg ggtcgttaca aaagactctt
      241 taagccaaga tttccttgac agccttggcg atagcatcgc caacctcagt ggtagagtta
      301 gaaccaccaa ggtcaccggt tctgacacct gcatccaaga catttctaac agcttcttca
      361 agagccctac cttcttcaac caaatccaag gataacttca acatcatagc tgcagataag
      421 atggtagcaa ttgggttaac cttgtttgct ggtaaatctg gggcagaacc atgacatggt
      481 tcgtacaaac cgaatgcctt gttagtgtca ggtagggaag ctagagatgc agaaggtaat
      541 aaacccaaag aacctggaat aacagaggct tcatcggaga taatatcacc aaacatgttg
      601 ttggtaataa caacaccgtt tagcttagtt ggtgatttaa ccaaaatcat agcagcagag
      661 tcgatcaatt ggtgctgaac agttaattgt gggaactcag tcttgatggt ttcttcaaca
      721 gtctttctcc acaatctgga agaggcaagc acgttagcct tgtcaagaga ccagattggt
      781 aatggtgggt tttgttgcaa tgccaagaaa gcagccattc ttgtaattct ttgaacttca
      841 ggaacactgt atttctcaga gtcccaagca actccgtcac cttcatcttc ttttctttca
      901 ccaaagtaga taccaccaac caattctcta acgacgacga aatcggtacc ctttgcatat
      961 tcaggcttca aaggagaaag atctagtaaa gaatcagaag caaagttaca tggtctcaag
     1021 ttggcgtata gacccaattc ctttctgatc ttcaatagac cttgttctgg tctaactgcg
     1081 cccgtacccc attttggacc accaacagca cctagtaaga cagcatcggc tttcttagag
     1141 gcttctagag cttcatctgg taaaggagtg ccagtggcat cgatggcagc acccccgatc
     1201 aagtgatgtt ggaaattgaa cttaatttct ggacggactt cagcaatggc attcaagacc
     1261 ttaatagctt cgtcagtaac ttctttaccg acgtgatcac ccggtaggac aacgatattc
     1321 ttagacatgg tataatctgt gtagtgtggg atacttttta cttcttcaaa taggtatcaa
     1381 caataaaaat aaatcgaagc aaatgtagga atgcgttaaa gcagatgtac ttacttagag
     1441 tacatagata tatttatata attcaatata taaaagtata tgaagcatct gatgttgaac
     1501 ctgtcatgac tctaaactgg aatgggtagt tatgcttctg aaggttttcc gttagaaaat
     1561 gagtgcaggg atcaggcccg gaaccggttt tagcctgaaa ggaaaagaaa aaaatgcggt
     1621 gattactagc acgtgactgc gctgaattgg aatccaccac aagtacagcg aatggcaaga
     1681 gggaagggga atatattgtg cctggcggta gtctgctata tcaaatgtta aacataacta
     1741 aatgcagtag tgaatagagt aatgtatttc tacatatgtg ggtagggcca atgggagccc
     1801 gatgtctatt gtacagatat tttcctttta tagttaatca attcaacttg gaaatcattg
     1861 agtcgatatc tacttggata actggagccg tggcagcgtt tgaaatttga cgagtcttct
     1921 tgttttgttc gcgcattttc agtttattca aacgggagat ggcaccgaat ccactgactt
     1981 ctctgttcaa tttgaatggt aataggaaga aagtacattt cgagaaattt tcgcccaacg
     2041 tcgaaatctc atggatgata tcctcgatac aaatgacacc ttcatcacct aatttctctt
     2101 caacgatatt gttgtcattc aagattatct cttttggttc tttatcttct ggcctttgcc
     2161 acattattct agatctcttt tggattaaag aacggataga tgcaagtgaa ggttgtccga
     2221 taacaatata tggtgataca agtttcaaca atgggtacac gtccttggta agtttgatga
     2281 aaacaccagt gttcaactca acaagtctca aaagagataa cactttgtaa gcctttgctg
     2341 ggatcttcac agcgagctct cgagaaccct taatataact tcgtataatg tatgctatac
     2401 gaagttatta ggtgatatca gatccactag tggcctatgc ggccgcggat ctgccggtct
     2461 ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc
     2521 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     2581 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2641 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2701 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2761 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2821 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2881 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     2941 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3001 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3061 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3121 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     3181 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3241 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3301 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3361 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3421 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3481 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3541 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3601 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3661 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3721 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3781 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3841 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3901 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3961 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4021 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4081 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4141 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4201 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4261 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4321 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4381 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4441 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     4501 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     4561 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     4621 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     4681 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     4741 atatggacat attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac
     4801 acatacgatt taggtgacac tata
//
