LOCUS       40924_26141        4107 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast shuttle vector pJHU2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Annaluru N.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2013) Environmental Health Sciences, Johns Hopkins
            School of Public Health, 615 North Wolfe St. E6402, Baltimore, MD 
            21205, USA
REFERENCE   2  (bases 1 to 4107)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4107)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-JUN-2013) Environmental Health Sciences, Johns Hopkins School of
            Public Health, 615 North Wolfe St. E6402, Baltimore, MD 21205, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    125..628
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        891..1106
                     /label=URA3 promoter
     CDS             1107..1907
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     protein_bind    2078..2094
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2102..2118
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2132..2188
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        2398..2489
                     /label=AmpR promoter
     CDS             2490..3347
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3354..3393
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      3425..4013
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4054..4075
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcct
      121 tttcatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt
      181 aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta
      241 aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag
      301 gtattaatgc cgaattgttt catcttgtct gtgtagaaga ccacacacga aaatcctgtg
      361 attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct
      421 aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt
      481 ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa
      541 acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga
      601 ggcgcgtgta agttacaggc aagcgatccg tcctaagaaa ccattattat catgacatta
      661 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
      721 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
      781 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
      841 aactatgcgg catcagagca gattgtactg agagtgcacc ataccacctt ttcaattcat
      901 catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt gattcggtaa
      961 tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca
     1021 tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa
     1081 aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta
     1141 ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact
     1201 tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag
     1261 gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg
     1321 gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca
     1381 gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa
     1441 tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg
     1501 gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag
     1561 aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact gttgacattg
     1621 cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag
     1681 atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg
     1741 cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct gacattatta
     1801 ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca
     1861 gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat
     1921 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt
     1981 tattacccta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     2041 aaattgtaaa cgttaatggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     2101 acaggaaaca gctatgacta tgattacgcc cgaattcgag ctcggtaccc ggggatcctc
     2161 tagagtcgac ctgcaggcat gcaagcttgg gcttgccgtc gttttacagc gacgagactg
     2221 ggaaaatcct ggcgttaccc aacttaatcg ccttgccgca cacccccctt tcgccagttg
     2281 gcgtaatagc gaagaagccc gcaccgaccg cccttcccaa cagttgcgta gtctgaatgg
     2341 cgaatggcgt taaactagta gcccgcctaa tgagcgggct tttttttaat tcccctattt
     2401 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2461 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2521 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctcgtgaaag
     2581 taaaagacgc agaggaccaa ttgggggcac gagtgggata catagaactg gacttgaata
     2641 gcggtaaaat ccttgagagt tttcgccctg aagagcgttt tccaatgatg agcactttca
     2701 aagttctgct atgtggagca gtattatccc gtgtagatgc ggggcaagag caactcggac
     2761 gacgaataca ctattcgcag aatgacttgg ttgaatactc cccagtgaca gaaaagcacc
     2821 ttacggacgg aatgacggta agagaattat gtagtgccgc cataacgatg agtgataaca
     2881 ctgcggcgaa cttacttctg acaaccatcg gtggaccgaa ggaattaacc gcttttttgc
     2941 acaatatggg agaccatgta actcgccttg accgttggga accagaactg aatgaagcca
     3001 taccaaacga cgagcgagac accacaatgc ctgcggcaat ggcaacaaca ttacgcaaac
     3061 tattaactgg cgaactactt actctggctt cacggcaaca attaatagac tggcttgaag
     3121 cggataaagt tgcaggacca ctactgcgtt cggcacttcc tgctggctgg tttattgctg
     3181 ataaatctgg ggcaggagag cgtggttcac ggggtatcat tgccgcactt ggaccagatg
     3241 gtaagccttc ccgtatcgta gttatctaca cgacgggtag tcaggcaact atggacgaac
     3301 gaaatagaca gattgctgaa ataggggctt cactgattaa gcattggtaa accgatacaa
     3361 ttaaaggctc cttttggagc cttttttttt ggacggaccg agtagaaaag atcaaaggat
     3421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3541 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3841 aagggacaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4021 gcaacgcggc ctttttacgg ttcctgaatt gattaatgtg agttagctca ctcattaggc
     4081 accccaggct ttacacttta tgctgcc
//
