LOCUS       40924_26131       11403 bp DNA     circular SYN 18-DEC-2018
DEFINITION  His-3 integration vector pJHAM004, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Lee DW, Haag JR, Aramayo R.
  TITLE     Construction of strains for rapid homokaryon purification after 
            integration of constructs at the histidine-3 (his-3) locus of 
            Neurospora crassa
  JOURNAL   Curr. Genet. 43 (1), 17-23 (2003)
  PUBMED    12684841
REFERENCE   2  (bases 1 to 11403)
  AUTHORS   Lee DW, Haag JR, Aramayo R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2002) Biology, Texas A
REFERENCE   3  (bases 1 to 11403)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11403)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Genet."; date: "2003"; volume: "43"; issue: "1"; pages: "17-23"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-DEC-2002) Biology, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             540..1331
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      1618..2206
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2494..2515
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(7517..7528)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     misc_feature    7793..7845
                     /label=multi-cloning sites
                     /note="multi-cloning sites"
ORIGIN
        1 gcggccgccg atagctagac tgggcggttt tacaacgtcg tgactgggaa aaccctggcg
       61 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      121 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgata
      181 agctagcttc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca
      241 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
      301 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
      361 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
      421 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
      481 ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca
      541 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
      601 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
      661 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc
      721 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
      781 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
      841 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
      901 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
      961 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     1021 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg
     1081 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     1141 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1201 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1261 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1321 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     1381 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     1441 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     1501 ccaccccaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1741 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     1801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1981 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     2041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2401 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2461 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2521 accccaggct ttacacttta tgcttccggc ttatgttgtg tggaattgtg agcgcggccg
     2581 cggccctctc ggcctcgaag cttgccatct ccaccatcct ctcgagcgtc tggaagtctg
     2641 accgtcccga tggtcttctc cccaccgttg tcgttgatga gcacgacact gctctgggtc
     2701 tggtctacag cagtgccgag agtgtgaacg aggccctcag gacacagact ggtgtctatc
     2761 agagccggaa gcgcggtctc tggtacaagg gtgctacttc cggagacact caggagctcg
     2821 tccgcatctc gcttgactgc gataacgatg ctctcaagtt tgtcgtgaag cagaagggtc
     2881 gtttctgcca cctcgatcag tccggctgct ttggtcagct caaaggcctt cccaagctcg
     2941 agcagacttt gatttcgagg aaacagtctg cccccgaggg ctcctacact gcccgtctct
     3001 tctccgatga gaagctagtc cgggccaaga tcatggagga ggctgaggag ctctgcaccg
     3061 ctcagacccc ccaggaaatc gcctttgagg ctgccgatct cttctacttt gctcttacca
     3121 gggccgttgc tgccggcgtt actcttgccg atatcgaaag gagccttgac gccaagagct
     3181 ggaaggtcaa gcgcaggact ggagatgcta agggtaagtg ggctgagaag gagggcatca
     3241 agcctgcggc gtccgctctc gctgccactt cggcccctgt caccaaggag gccgcccagg
     3301 agaccacccc tgagaagatc accatgagac gtttcgacgc ctccaaggtc tctaccgagg
     3361 agctcgatgc tgctctcaag cgtcctgcgc aaaagtcgtc cgatgccatc tacaagatca
     3421 ttgtccccat catcgaggac gtccgcaaga acggcgacaa ggctgttctg tcgtacactc
     3481 acaagttcga gaaggctacc tctcttacta gccccgtcct gaaggcgccc ttccccaagg
     3541 agcttatgca gctccctgag gagaccattg ctgccatcga cgtgtccttc gagaacatcc
     3601 gcaagttcca cgccgcccag aaggaggaga agcccctcca ggtcgagacc atgcccggtg
     3661 ttgtctgcag ccgtttctct cgtcccatcg aggccgtcgg ctgctacatc cccggcggta
     3721 ccgccgttct ccccagcact gcccttatgc tgggtgttcc cgccatggtc gccggctgca
     3781 acaagattgt gttcgcctct cctccccgcg ccgacggaac catcactccc gagattgtct
     3841 acgtcgctca caaggttggc gccgagtcca tcgtgcttgc cggcggtgcc caggccgtag
     3901 ctgccatggc ctacggcacc gagagcatca ccaaggtcga caagattctc ggccccggta
     3961 accagttcgt cactgctgcc aagatgttcg tcagcaacga caccaacgct gccgttggta
     4021 ttgacatgcc cgctggcccg tccgaggtgc tggtcatcgc tgacaaggac gccaaccccg
     4081 cgttcgttgc ctcggatctc ctgtcccagg ctgagcacgg cgttgacagt caggtcatcc
     4141 tgatcgctat tgacctcgac gaggagcatc ttcaggctat tgaggacgag gttcaccgtc
     4201 aggctacgga gcttcctcgc gtccagattg tccgtggctc catcgcccac tcgatcaccg
     4261 tgcaggtcaa gaccgtcgag gaggccatgg agctcagcaa caagtacgct cctgagcact
     4321 tgatcctcca gatcaaggag gccgagaagg ctgtcgatct tgtcatgaac gccggtagtg
     4381 tcttcattgg cgcctggact cctgagtccg ttggcgatta ctctgctggt gttaaccact
     4441 cgctgcgtaa gttacatatc ataaatagcc ccgcttcaca gattcttctg ctaacgtcaa
     4501 gacacatagc tacctatggc tttgccaagc agtactctgg cgtcaatctc gcctcgttcg
     4561 tcaagcacat taccagctcc aacttgactg ccgagggtct caaaaacgtc ggccaggctg
     4621 tcatgcagtt ggctaaggtt gaggagctcg aggctcacag aagggcggtc agcatccgtc
     4681 ttgagcacat gagcaagagc aactaaacgg aaattctttt cgaagtagca aaaaaaaaaa
     4741 aaaaaaacaa gaacaaaagg atgtagtggg ttgatgtata tctgggtcat tttgggcaca
     4801 tagagtaatg ataacgagtt ttggacattg tactgttctg tacaggctga agatcagtac
     4861 atgaatctgt tggtaagtgt agagacccaa acgtcccttt agtttttctc cctattccag
     4921 aaggggtgct cgtccctggg tgtttatttt cattattaca tcaacttttt tttttttttt
     4981 ttttttttca gatcatgcgt acatgaacgg ggaagcacag acgatcgaaa cgtggatgtc
     5041 acaatgtcgc tgcagtgatg ctgcattgca tgaagcgccc atctcaatat acttgcagtc
     5101 ttgcacgttg catgtgaacc tcccaaacaa ccgaataaaa gacggcgaaa aatgaagata
     5161 aaaaaaaacc ataaaaaaaa tcagagggag tgtgggaaat ggtgtctttt agcattcaga
     5221 ccccatagcc gtgcacgccc gggtacagac aggttcatcg atgttgacat tgactgggac
     5281 accaggtcta tctattttat ctcctgtcct ctaccataca tcgggacatc ggacatcttg
     5341 ctgtaccccc cacacccaca aagcctcata aaagcgccac acccgaggag gttcggtcgg
     5401 ccccacgaac tctgtgcctc cctgcctgtt tacagggacc gaacgctgga gaatcttact
     5461 agtttcctga catccggcct acccgagcag gaaaagggac agctcatagg cgaggaggga
     5521 tttgaagatg ggaacatttt gggtgattcg agaggaggaa ctaggtactg catcatgata
     5581 gttcggggca gcatcttggc tgggacattg ttaatacctc gatatgatga agtaggaggg
     5641 agtttttgcg tgtcttgccg aagtccagag atctgtttta ttttattttt tatggatgta
     5701 gtgtatcaac acccaagatt cggagaatag tactaggatt cgcatttaca agtggaagtc
     5761 ttgagaatcg ttgtatatcc ttgtcttcct cggaatgtta acaatcctac agcgagcgag
     5821 cgagcggtcg gatgcgctga tctgataggc gcaatatacg gccgctttct ccggtcgtgt
     5881 agtgtaagct ctgtgggcat agtacactaa aaaaaccctt gcatttcatg atctgcctgc
     5941 tattcattcc gagctatttc agtggtcaca tttcgaggaa gaaagaaagc aactaagatt
     6001 cacagccatc catccatcca tatggaagaa taatccattc ccatgttccc tcccccccac
     6061 tatgtatgtg accacacgct gctgtcagaa tgccaacggt ctcaggtacc ctcgtccgac
     6121 tgtttggcat ggagttacat acactactag tgtagccccg ggccaagcta ccccgtcaaa
     6181 tctatacata tctataacgg gtttcagggg tttcgttcgc tgtcaatcaa gtttgaaaca
     6241 tcactggggc cgttggacgg tgtattagac cattggctcc ctcagctgtt tggcggctgg
     6301 gcggctgggt caaacggcaa taacgggact cgagagggac gaggagagtc ggttggctgg
     6361 ctgcaataca agcgttccca cctaaccaac gagtcccgtt ttccatttgt gtgcctaacc
     6421 atcatctagg gatgtcaggg tttggccgga tcagggtatg tttggttgac tgttgtcatg
     6481 tctgattggg tacatattat ggtaggtgtc tcgagaacag tagagtactc gggcctagcg
     6541 tttggatgat tacgcgagat atgagttgtg ggccgccatg cagttgcttg tccataagca
     6601 gaagttgctt tgggatatat ttctcgtctt tcaaaggtca cgaggtcctg ggacgaacgg
     6661 catcgccatc caaagggttg aacatgagaa acctgaatgg cctttgcgtt gaaatacaaa
     6721 aagtcaagaa caaaatcgct tgaggatagg gacgtggaag caagcaaata tggtaagaga
     6781 ggtatacatc aaccctggtt caattgttag cgtggttctt cctccacgtc ctcgttcatg
     6841 acggttaaca gtaccaggct aacaattaaa ccagggttga tgtgtactga tatgtaggtg
     6901 ctcagcaaac tgccaatttc ttggccccaa gcagcagttt gctgtcagtg ctgctcgtgt
     6961 cagccttggt agtggaacct aaactgctaa cacagcgcaa gtgcgcatgt aaagatattg
     7021 tgggaggatc tgtatggatg gatgagatta ctgcttggtg ttggttgcga ggcactgcgg
     7081 ctgttaggct ttgctgtgcc ccgttcgacg aagaaatacg cggaactata aattggatac
     7141 ctagacttac tgcctatggg aggtatctac cgacgtagcc gacggattct agcaacatcc
     7201 cgactttgct tgtagtgtac tatgatagca gcacagtgtt gctccttgtg agaatgggct
     7261 cttttttttt tttccccctt ccctagggcg ttgactggac ttgctctatt gttcccaagg
     7321 taggtgcccg tcatcgattt tcccaagtct cccgccagat tgtcgtcata gtgtcatgat
     7381 gacctcggtc gctggggctg cgtggttacg gggagctggg accgctaggc ctcagtggtt
     7441 gtgccattca gcgtgggtgt gtggagtagc ggtagaggcg cttggaagtt gtgctagcgg
     7501 aaaccctgga atatcttcta ccctcgattc cttctcgggc tgcccatgtg ctgaggtgat
     7561 gccggggatc tggcgccaat catccattga ggttcccgca gcttcccggt gccgcgcgcg
     7621 ggcgcagttg ctcacaggac acacctagac gcaggggcac aggggcaccg tttggtgtgc
     7681 aactgggtac ctagctgtag caagcactcc accgtctgtg caatccccca atccacggca
     7741 ggaacttcgc accgccgcgg caccgagtga gcgaatccat ccgcattgga tcgtttaaac
     7801 tctagagcat gccccgggat cctgatcatc gatgggcccg aattcaaaat accacgggcg
     7861 caggtggtat tggaggcttg ttgcagtcca gcacatattt gtatgtaaag tggttcttct
     7921 tatctcgttt tcgagtgtca actgcgccag ttcagagttg ggcggctgtg gacgaccttg
     7981 ctggtgaaca tgtcttggac tccatgcccc ttcttcgttt cctcaaatca agaagtcgag
     8041 gaccgtgacc gtaaatcgct aacgcaactc tagggccgga ctttctggtg gtggctggct
     8101 tgtcggcagt ttgttctcca acaacttcag cagcattgag accctgctga gcgagaacaa
     8161 agtctgggac tttgagaact ccatctttaa agggcccaag gaggctggcc ttagtactgt
     8221 caaccgcatt cagtactggt ccgaagtggc aaaggaagtt gccaagaaga aggatgctgg
     8281 cttcgagaca agtataacag actactgggg ccgagcattg agttaccaac tgatcggagc
     8341 cgatatgggc ggcccggctt acaccttctc cagcattgcc cagaccgaca acttccagaa
     8401 ggccgaaacg ccgttcccta ttctggtagc tgacggccgc gcgcctggag acaccatcat
     8461 ctccctcaat gctaccaact acgagttcaa cccgttcgag acgggtagct gggacccgac
     8521 cgtctatggc tttgcgccga ccaagtacct cggcgccaac ttcagcaacg gcgtgatccc
     8581 atcgggaggc aagtgcgttg agggtctcga ccaagccggc ttcgtcatgg gcaccagcag
     8641 cacgctcttc aaccagttcc ttttggccaa catctccagc tacgacggtg tgcccgacgt
     8701 gctcatcgag gccgtgactt ctgtcctcaa ggaaatcggc gccaagaggg acgacgtctc
     8761 ccaaatcatc cctaatccgt tcctggactg gaacaaccgg accaacccca acgccgacac
     8821 gctcgagctc gacctggtcg acggcggcga agatctgcag aatattccgc tcaacccgct
     8881 cacccaaccc gtgcgcgccg tggacgtcat cttcgctgtc gactcgtccg ccgacgtgac
     8941 aaactggccc aatggcaccg ccctgcgagc cacctacgag cgcactttcg gctctatttc
     9001 caacgggaca ctcttcccct cgatccccga cgactggacg tttataaacc taggcctcaa
     9061 caaccgcccc tctttcttcg gctgcgatgt taagaacttt accttgaacg ccaaccaaaa
     9121 ggttcccccc ttaatcgtct atgtccccaa cgcgccctat accgcgctga gcaacgtgtc
     9181 caccttcgat ccgtcataca ccatgtctca gcgcaacgac atcatcggca acggatggaa
     9241 ctcagccacg cagggaaacg gcacgctgga ttcggagtgg cccacttgcg tcgcctgcgc
     9301 ggttatcagc aggagcttag atcggttggg caggcagacg ccagccgcgt gcaagacttg
     9361 ctttgagagg tattgctgga atggcacagt gaactcaaaa gatacagggg tttacatgcc
     9421 tgagttcaag attgcggatg cgcatgccct ggactcgggt gctgttgcta tcggaaagat
     9481 ggtgaatgtc tggtcgtcgg ttgtggtggg agttgtggcg gctactttgt tgttgtaggg
     9541 gtaggggaga cgtgatgata ttccagtctg atgaagttga gactggactg gagatcgcca
     9601 aggatgcgga gggaaaggaa tgcgtggtgt taatgtcatg atggatgaag ggtcatggat
     9661 catggaacga cggggcgggg atattggatg atggatatac cacactgcat gcatgctcta
     9721 ttgatagtat gctttggcat ttacgtttaa caatcaattg ctccatcctg atgttctatc
     9781 tttttcgaca ctggattgat actactcctg ttgcttcgct cttgaggttg gaaggacctt
     9841 gaggttggaa ggaccttgag gttgtttgtt ctgagggagg ttatcgaagt atcatctgtg
     9901 ctgatgccga tcgatagact gccctcttct tcgaggcaac gaacggtcgg atgagcctct
     9961 aatcatgatg ctcagtgcca caaaaaggct ccagcacagc tgcccacacc ttttttgcct
    10021 cgtcgctcct tccttttttt cccccccttt cttcctttcc atctcatccc gtaccagagt
    10081 gcccaccggg tatatatatt acctccttgg ccgttctcct ttgaccaata aatcgcttgg
    10141 tcgagtggcg taaccgttta ccgtctacac ttatcactca aaccaaacca aaccatcgaa
    10201 gaagttacct atcggttcga gggaacggtg atgttcttac gttcaagtaa cccaaagagc
    10261 gttccacatc gttgaccgtc tcctccagtt cttggatctg tttaacttcc gcagcgactg
    10321 aagaaggtaa tcactttttt tttttttggt tcaaaaaaaa aaaaaaaaaa ttacacgaaa
    10381 aggttagagg cgtttcgatg gttaaactcg atcgagcggt tgtttgtagc cttttctttt
    10441 gtgtcttttg gcagtacttc ctttggaggt ggaaggaggg ttagaggccg cgtcaaccta
    10501 agtaagggag ggagctggtt gcattcgaac cccggatggg acttgtgttc tgttgttgtt
    10561 tttttttgca ctgatgatga ttctgggcga gtgcgctttt gataactaca gtcccacgtc
    10621 atcaacttca cgattagtcc ttgactcttt tttttctttt tctactaaaa gcctggtctt
    10681 acccaagtgt tcacggtata tggagtatgc gatagagggc gatatatctt ctcaacaact
    10741 cgtttcttct cgctcagccc acaatagggt gcttgtaatt cttccaccga gagcaagtgg
    10801 gagtaatctt cttcgatggc tacagccgtc agtcagacca cctacatcca cgtccaggca
    10861 atatctctgg gacttcacaa caacaaaagc gcactggaat gaaacaggaa atttctcgag
    10921 ataagtgcct gattgtagtc ttgcggttca tgtacatctg accttttctt gagagccccc
    10981 gtttctggtc caaaatcgtc tgactttcac ctagcctgcc cccacgaaat ccagtctccc
    11041 cttgatgtcg tcgggcaacg tcgagagata ctcctcccaa atgtcctgat ttgcacccgc
    11101 gggcacgcgg aacgtgtaaa taagtcctcc tgttcctcct tttcttccac tagcttcgtc
    11161 atctccagcc atcaccgcca actctcggtt tatcttcggt gccagaatga tgcttgatgt
    11221 cctaggactg cccgcaaatt gcagatacaa gggaacagga ggacagctac taagcggcgg
    11281 cgacaatgac attcccccca cgcccgagcc gagcagttcc tggactctag cccccatggt
    11341 caggggaggg gatgttgtca ctctagcatg aagatcagcg atcgagatgc cccccaaaag
    11401 ctt
//
