LOCUS       40924_26121       11373 bp DNA     circular SYN 18-DEC-2018
DEFINITION  His-3 integration vector pJHAM002, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Lee DW, Haag JR, Aramayo R.
  TITLE     Construction of strains for rapid homokaryon purification after 
            integration of constructs at the histidine-3 (his-3) locus of 
            Neurospora crassa
  JOURNAL   Curr. Genet. 43 (1), 17-23 (2003)
  PUBMED    12684841
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   Lee DW, Haag JR, Aramayo R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2002) Biology, Texas A
REFERENCE   3  (bases 1 to 11373)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11373)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Genet."; date: "2003"; volume: "43"; issue: "1"; pages: "17-23"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-DEC-2002) Biology, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             540..1331
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      1618..2206
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2494..2515
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    6156..6183
                     /label=multi-cloning sites
                     /note="multi-cloning sites"
     CDS             6443..6454
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
ORIGIN
        1 gcggccgccg atagctagac tgggcggttt tacaacgtcg tgactgggaa aaccctggcg
       61 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      121 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgata
      181 agctagcttc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca
      241 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
      301 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
      361 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
      421 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
      481 ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca
      541 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
      601 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
      661 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc
      721 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
      781 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
      841 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
      901 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
      961 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     1021 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg
     1081 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     1141 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1201 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1261 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1321 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     1381 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     1441 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     1501 ccaccccaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1741 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     1801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1981 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     2041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2401 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2461 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2521 accccaggct ttacacttta tgcttccggc ttatgttgtg tggaattgtg agcgcggccg
     2581 cggccctctc ggcctcgaag cttttggggg gcatctcgat cgctgatctt catgctagag
     2641 tgacaacatc ccctcccctg accatggggg ctagagtcca ggaactgctc ggctcgggcg
     2701 tggggggaat gtcattgtcg ccgccgctta gtagctgtcc tcctgttccc ttgtatctgc
     2761 aatttgcggg cagtcctagg acatcaagca tcattctggc accgaagata aaccgagagt
     2821 tggcggtgat ggctggagat gacgaagcta gtggaagaaa aggaggaaca ggaggactta
     2881 tttacacgtt ccgcgtgccc gcgggtgcaa atcaggacat ttgggaggag tatctctcga
     2941 cgttgcccga cgacatcaag gggagactgg atttcgtggg ggcaggctag gtgaaagtca
     3001 gacgattttg gaccagaaac gggggctctc aagaaaaggt cagatgtaca tgaaccgcaa
     3061 gactacaatc aggcacttat ctcgagaaat ttcctgtttc attccagtgc gcttttgttg
     3121 ttgtgaagtc ccagagatat tgcctggacg tggatgtagg tggtctgact gacggctgta
     3181 gccatcgaag aagattactc ccacttgctc tcggtggaag aattacaagc accctattgt
     3241 gggctgagcg agaagaaacg agttgttgag aagatatatc gccctctatc gcatactcca
     3301 tataccgtga acacttgggt aagaccaggc ttttagtaga aaaagaaaaa aaagagtcaa
     3361 ggactaatcg tgaagttgat gacgtgggac tgtagttatc aaaagcgcac tcgcccagaa
     3421 tcatcatcag tgcaaaaaaa aacaacaaca gaacacaagt cccatccggg gttcgaatgc
     3481 aaccagctcc ctcccttact taggttgacg cggcctctaa ccctccttcc acctccaaag
     3541 gaagtactgc caaaagacac aaaagaaaag gctacaaaca accgctcgat cgagtttaac
     3601 catcgaaacg cctctaacct tttcgtgtaa tttttttttt ttttttttga accaaaaaaa
     3661 aaaaaagtga ttaccttctt cagtcgctgc ggaagttaaa cagatccaag aactggagga
     3721 gacggtcaac gatgtggaac gctctttggg ttacttgaac gtaagaacat caccgttccc
     3781 tcgaaccgat aggtaacttc ttcgatggtt tggtttggtt tgagtgataa gtgtagacgg
     3841 taaacggtta cgccactcga ccaagcgatt tattggtcaa aggagaacgg ccaaggaggt
     3901 aatatatata cccggtgggc actctggtac gggatgagat ggaaaggaag aaaggggggg
     3961 aaaaaaagga aggagcgacg aggcaaaaaa ggtgtgggca gctgtgctgg agcctttttg
     4021 tggcactgag catcatgatt agaggctcat ccgaccgttc gttgcctcga agaagagggc
     4081 agtctatcga tcggcatcag cacagatgat acttcgataa cctccctcag aacaaacaac
     4141 ctcaaggtcc ttccaacctc aaggtccttc caacctcaag agcgaagcaa caggagtagt
     4201 atcaatccag tgtcgaaaaa gatagaacat caggatggag caattgattg ttaaacgtaa
     4261 atgccaaagc atactatcaa tagagcatgc atgcagtgtg gtatatccat catccaatat
     4321 ccccgccccg tcgttccatg atccatgacc cttcatccat catgacatta acaccacgca
     4381 ttcctttccc tccgcatcct tggcgatctc cagtccagtc tcaacttcat cagactggaa
     4441 tatcatcacg tctcccctac ccctacaaca acaaagtagc cgccacaact cccaccacaa
     4501 ccgacgacca gacattcacc atctttccga tagcaacagc acccgagtcc agggcatgcg
     4561 catccgcaat cttgaactca ggcatgtaaa cccctgtatc ttttgagttc actgtgccat
     4621 tccagcaata cctctcaaag caagtcttgc acgcggctgg cgtctgcctg cccaaccgat
     4681 ctaagctcct gctgataacc gcgcaggcga cgcaagtggg ccactccgaa tccagcgtgc
     4741 cgtttccctg cgtggctgag ttccatccgt tgccgatgat gtcgttgcgc tgagacatgg
     4801 tgtatgacgg atcgaaggtg gacacgttgc tcagcgcggt atagggcgcg ttggggacat
     4861 agacgattaa ggggggaacc ttttggttgg cgttcaaggt aaagttctta acatcgcagc
     4921 cgaagaaaga ggggcggttg ttgaggccta ggtttataaa cgtccagtcg tcggggatcg
     4981 aggggaagag tgtcccgttg gaaatagagc cgaaagtgcg ctcgtaggtg gctcgcaggg
     5041 cggtgccatt gggccagttt gtcacgtcgg cggacgagtc gacagcgaag atgacgtcca
     5101 cggcgcgcac gggttgggtg agcgggttga gcggaatatt ctgcagatct tcgccgccgt
     5161 cgaccaggtc gagctcgagc gtgtcggcgt tggggttggt ccggttgttc cagtccagga
     5221 acggattagg gatgatttgg gagacgtcgt ccctcttggc gccgatttcc ttgaggacag
     5281 aagtcacggc ctcgatgagc acgtcgggca caccgtcgta gctggagatg ttggccaaaa
     5341 ggaactggtt gaagagcgtg ctgctggtgc ccatgacgaa gccggcttgg tcgagaccct
     5401 caacgcactt gcctcccgat gggatcacgc cgttgctgaa gttggcgccg aggtacttgg
     5461 tcggcgcaaa gccatagacg gtcgggtccc agctacccgt ctcgaacggg ttgaactcgt
     5521 agttggtagc attgagggag atgatggtgt ctccaggcgc gcggccgtca gctaccagaa
     5581 tagggaacgg cgtttcggcc ttctggaagt tgtcggtctg ggcaatgctg gagaaggtgt
     5641 aagccgggcc gcccatatcg gctccgatca gttggtaact caatgctcgg ccccagtagt
     5701 ctgttatact tgtctcgaag ccagcatcct tcttcttggc aacttccttt gccacttcgg
     5761 accagtactg aatgcggttg acagtactaa ggccagcctc cttgggccct ttaaagatgg
     5821 agttctcaaa gtcccagact ttgttctcgc tcagcagggt ctcaatgctg ctgaagttgt
     5881 tggagaacaa actgccgaca agccagccac caccagaaag tccggcccta gagttgcgtt
     5941 agcgatttac ggtcacggtc ctcgacttct tgatttgagg aaacgaagaa ggggcatgga
     6001 gtccaagaca tgttcaccag caaggtcgtc cacagccgcc caactctgaa ctggcgcagt
     6061 tgacactcga aaacgagata agaagaacca ctttacatac aaatatgtgc tggactgcaa
     6121 caagcctcca ataccacctg cgcccgtggt attttgaatt cggtctagag tttaaacgga
     6181 tccaatgcgg atggattcgc tcactcggtg ccgcggcggt gcgaagttcc tgccgtggat
     6241 tgggggattg cacagacggt ggagtgcttg ctacagctag gtacccagtt gcacaccaaa
     6301 cggtgcccct gtgcccctgc gtctaggtgt gtcctgtgag caactgcgcc cgcgcgcggc
     6361 accgggaagc tgcgggaacc tcaatggatg attggcgcca gatccccggc atcacctcag
     6421 cacatgggca gcccgagaag gaatcgaggg tagaagatat tccagggttt ccgctagcac
     6481 aacttccaag cgcctctacc gctactccac acacccacgc tgaatggcac aaccactgag
     6541 gcctagcggt cccagctccc cgtaaccacg cagccccagc gaccgaggtc atcatgacac
     6601 tatgacgaca atctggcggg agacttggga aaatcgatga cgggcaccta ccttgggaac
     6661 aatagagcaa gtccagtcaa cgccctaggg aagggggaaa aaaaaaaaag agcccattct
     6721 cacaaggagc aacactgtgc tgctatcata gtacactaca agcaaagtcg ggatgttgct
     6781 agaatccgtc ggctacgtcg gtagatacct cccataggca gtaagtctag gtatccaatt
     6841 tatagttccg cgtatttctt cgtcgaacgg ggcacagcaa agcctaacag ccgcagtgcc
     6901 tcgcaaccaa caccaagcag taatctcatc catccataca gatcctccca caatatcttt
     6961 acatgcgcac ttgcgctgtg ttagcagttt aggttccact accaaggctg acacgagcag
     7021 cactgacagc aaactgctgc ttggggccaa gaaattggca gtttgctgag cacctacata
     7081 tcagtacaca tcaaccctgg tttaattgtt agcctggtac tgttaaccgt catgaacgag
     7141 gacgtggagg aagaaccacg ctaacaattg aaccagggtt gatgtatacc tctcttacca
     7201 tatttgcttg cttccacgtc cctatcctca agcgattttg ttcttgactt tttgtatttc
     7261 aacgcaaagg ccattcaggt ttctcatgtt caaccctttg gatggcgatg ccgttcgtcc
     7321 caggacctcg tgacctttga aagacgagaa atatatccca aagcaacttc tgcttatgga
     7381 caagcaactg catggcggcc cacaactcat atctcgcgta atcatccaaa cgctaggccc
     7441 gagtactcta ctgttctcga gacacctacc ataatatgta cccaatcaga catgacaaca
     7501 gtcaaccaaa cataccctga tccggccaaa ccctgacatc cctagatgat ggttaggcac
     7561 acaaatggaa aacgggactc gttggttagg tgggaacgct tgtattgcag ccagccaacc
     7621 gactctcctc gtccctctcg agtcccgtta ttgccgtttg acccagccgc ccagccgcca
     7681 aacagctgag ggagccaatg gtctaataca ccgtccaacg gccccagtga tgtttcaaac
     7741 ttgattgaca gcgaacgaaa cccctgaaac ccgttataga tatgtataga tttgacgggg
     7801 tagcttggcc cggggctaca ctagtagtgt atgtaactcc atgccaaaca gtcggacgag
     7861 ggtacctgag accgttggca ttctgacagc agcgtgtggt cacatacata gtggggggga
     7921 gggaacatgg gaatggatta ttcttccata tggatggatg gatggctgtg aatcttagtt
     7981 gctttctttc ttcctcgaaa tgtgaccact gaaatagctc ggaatgaata gcaggcagat
     8041 catgaaatgc aagggttttt ttagtgtact atgcccacag agcttacact acacgaccgg
     8101 agaaagcggc cgtatattgc gcctatcaga tcagcgcatc cgaccgctcg ctcgctcgct
     8161 gtaggattgt taacattccg aggaagacaa ggatatacaa cgattctcaa gacttccact
     8221 tgtaaatgcg aatcctagta ctattctccg aatcttgggt gttgatacac tacatccata
     8281 aaaaataaaa taaaacagat ctctggactt cggcaagaca cgcaaaaact ccctcctact
     8341 tcatcatatc gaggtattaa caatgtccca gccaagatgc tgccccgaac tatcatgatg
     8401 cagtacctag ttcctcctct cgaatcaccc aaaatgttcc catcttcaaa tccctcctcg
     8461 cctatgagct gtcccttttc ctgctcgggt aggccggatg tcaggaaact agtaagattc
     8521 tccagcgttc ggtccctgta aacaggcagg gaggcacaga gttcgtgggg ccgaccgaac
     8581 ctcctcgggt gtggcgcttt tatgaggctt tgtgggtgtg gggggtacag caagatgtcc
     8641 gatgtcccga tgtatggtag aggacaggag ataaaataga tagacctggt gtcccagtca
     8701 atgtcaacat cgatgaacct gtctgtaccc gggcgtgcac ggctatgggg tctgaatgct
     8761 aaaagacacc atttcccaca ctccctctga ttttttttat ggtttttttt tatcttcatt
     8821 tttcgccgtc ttttattcgg ttgtttggga ggttcacatg caacgtgcaa gactgcaagt
     8881 atattgagat gggcgcttca tgcaatgcag catcactgca gcgacattgt gacatccacg
     8941 tttcgatcgt ctgtgcttcc ccgttcatgt acgcatgatc tgaaaaaaaa aaaaaaaaaa
     9001 aaaaagttga tgtaataatg aaaataaaca cccagggacg agcacccctt ctggaatagg
     9061 gagaaaaact aaagggacgt ttgggtctct acacttacca acagattcat gtactgatct
     9121 tcagcctgta cagaacagta caatgtccaa aactcgttat cattactcta tgtgcccaaa
     9181 atgacccaga tatacatcaa cccactacat ccttttgttc ttgttttttt tttttttttt
     9241 tgctacttcg aaaagaattt ccgtttagtt gctcttgctc atgtgctcaa gacggatgct
     9301 gaccgccctt ctgtgagcct cgagctcctc aaccttagcc aactgcatga cagcctggcc
     9361 gacgtttttg agaccctcgg cagtcaagtt ggagctggta atgtgcttga cgaacgaggc
     9421 gagattgacg ccagagtact gcttggcaaa gccataggta gctatgtgtc ttgacgttag
     9481 cagaagaatc tgtgaagcgg ggctatttat gatatgtaac ttacgcagcg agtggttaac
     9541 accagcagag taatcgccaa cggactcagg agtccaggcg ccaatgaaga cactaccggc
     9601 gttcatgaca agatcgacag ccttctcggc ctccttgatc tggaggatca agtgctcagg
     9661 agcgtacttg ttgctgagct ccatggcctc ctcgacggtc ttgacctgca cggtgatcga
     9721 gtgggcgatg gagccacgga caatctggac gcgaggaagc tccgtagcct gacggtgaac
     9781 ctcgtcctca atagcctgaa gatgctcctc gtcgaggtca atagcgatca ggatgacctg
     9841 actgtcaacg ccgtgctcag cctgggacag gagatccgag gcaacgaacg cggggttggc
     9901 gtccttgtca gcgatgacca gcacctcgga cgggccagcg ggcatgtcaa taccaacggc
     9961 agcgttggtg tcgttgctga cgaacatctt ggcagcagtg acgaactggt taccggggcc
    10021 gagaatcttg tcgaccttgg tgatgctctc ggtgccgtag gccatggcag ctacggcctg
    10081 ggcaccgccg gcaagcacga tggactcggc gccaaccttg tgagcgacgt agacaatctc
    10141 gggagtgatg gttccgtcgg cgcggggagg agaggcgaac acaatcttgt tgcagccggc
    10201 gaccatggcg ggaacaccca gcataagggc agtgctgggg agaacggcgg taccgccggg
    10261 gatgtagcag ccgacggcct cgatgggacg agagaaacgg ctgcagacaa caccgggcat
    10321 ggtctcgacc tggaggggct tctcctcctt ctgggcggcg tggaacttgc ggatgttctc
    10381 gaaggacacg tcgatggcag caatggtctc ctcagggagc tgcataagct ccttggggaa
    10441 gggcgccttc aggacggggc tagtaagaga ggtagccttc tcgaacttgt gagtgtacga
    10501 cagaacagcc ttgtcgccgt tcttgcggac gtcctcgatg atggggacaa tgatcttgta
    10561 gatggcatcg gacgactttt gcgcaggacg cttgagagca gcatcgagct cctcggtaga
    10621 gaccttggag gcgtcgaaac gtctcatggt gatcttctca ggggtggtct cctgggcggc
    10681 ctccttggtg acaggggccg aagtggcagc gagagcggac gccgcaggct tgatgccctc
    10741 cttctcagcc cacttaccct tagcatctcc agtcctgcgc ttgaccttcc agctcttggc
    10801 gtcaaggctc ctttcgatat cggcaagagt aacgccggca gcaacggccc tggtaagagc
    10861 aaagtagaag agatcggcag cctcaaaggc gatttcctgg ggggtctgag cggtgcagag
    10921 ctcctcagcc tcctccatga tcttggcccg gactagcttc tcatcggaga agagacgggc
    10981 agtgtaggag ccctcggggg cagactgttt cctcgaaatc aaagtctgct cgagcttggg
    11041 aaggcctttg agctgaccaa agcagccgga ctgatcgagg tggcagaaac gacccttctg
    11101 cttcacgaca aacttgagag catcgttatc gcagtcaagc gagatgcgga cgagctcctg
    11161 agtgtctccg gaagtagcac ccttgtacca gagaccgcgc ttccggctct gatagacacc
    11221 agtctgtgtc ctgagggcct cgttcacact ctcggcactg ctgtagacca gacccagagc
    11281 agtgtcgtgc tcatcaacga caacggtggg gagaagacca tcgggacggt cagacttcca
    11341 gacgctcgag aggatggtgg agatggcaag ctt
//
