LOCUS       40924_26116        7044 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJH391, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Using lambda repressor fusions to isolate and characterize 
            self-assembling domains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Hu JC.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7044)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7044)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-OCT-2000) Biochemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..58
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    66..82
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      91..94
                     /regulatory_class="ribosome_binding_site"
     CDS             103..531
                     /codon_start=1
                     /product="cI DNA binding domain"
                     /label=cI DNA binding domain
                     /note="cI-DBD"
                     /protein_id="AAK07889.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTDRSRRFTTS"
     CDS             2438..2503
                     /label=GCN4_v1
                     /note="GCN4 peptide that allows binding to an scFv-GFP
                     fusion protein (Tanenbaum et al., 2014)"
     terminator      2573..2620
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             4105..4293
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    4398..4538
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4724..5312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5423..5936
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(5981..6838)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6839..6943)
                     /label=AmpR promoter
ORIGIN
        1 aattctcact cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
       61 tggaattgtg agcggataac aatttcacac aggaaacagc gtatgagcac aaaaaagaaa
      121 ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta tgaaaaaaag
      181 aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat ggggcagtca
      241 ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc cgcattgctt
      301 gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag agaaatctac
      361 gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga gtaccctgtt
      421 ttttctcatg ttcaggcagg gatgttctca cctaagctta gaacctttac caaaggtgat
      481 gcggagagat gggtgtcgac ggatcgatcc cgtcgtttta caacgtcgtg actgggaaaa
      541 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      601 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      661 gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct
      721 tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc
      781 catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa
      841 tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca
      901 gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg
      961 ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg
     1021 cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg agtgacggca gttatctgga
     1081 agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc
     1141 gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc
     1201 tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt
     1261 ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat
     1321 tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc
     1381 gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc
     1441 cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga
     1501 aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga
     1561 gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct
     1621 gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg
     1681 gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca
     1741 cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga
     1801 acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct
     1861 ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt
     1921 cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat
     1981 tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg
     2041 gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata
     2101 cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca
     2161 gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata
     2221 tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga
     2281 tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac
     2341 ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt
     2401 gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgtctaaaa actaccacct
     2461 cgagaacgaa gttgcgcgcc tgaaaaaact agttggtgaa cgttgaggat ccggcgctgc
     2521 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     2581 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatg
     2641 cccggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     2701 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     2761 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     2821 cacacccgtc ctgtggatct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     2881 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     2941 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
     3001 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
     3061 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
     3121 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
     3181 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
     3241 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
     3301 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg
     3361 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc
     3421 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca
     3481 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
     3541 gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg
     3601 cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct
     3661 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
     3721 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
     3781 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
     3841 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
     3901 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
     3961 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
     4021 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
     4081 ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct taacatggcc
     4141 cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat
     4201 gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc
     4261 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     4321 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     4381 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     4441 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     4501 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     4561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     4921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5161 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5401 ggatcttcac ctagatcctt ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5461 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5521 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5581 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     5641 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5701 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5761 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5821 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa
     5881 atatttgctt atacaatctt cctgtttttg gggcttttct gattatcaac cggggtaaat
     5941 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6001 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6061 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6121 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6181 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6241 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     6301 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6361 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6421 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6481 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6541 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     6601 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6661 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6721 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6781 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6841 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6901 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6961 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     7021 tcacgaggcc ctttcgtctt caag
//
