LOCUS       V005333                 5859 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005333
VERSION     V005333
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Goodson JR, Klupt SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 5859)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5859)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    397..1389
                     /note="LoaP Region Homology; LoaP region homology for
                     integration containing dfnA sequence"
     gene            complement(397..972)
                     /pseudo=""
                     /gene="dfnA"
                     /label="dfnA"
                     /note="fragment"
     5'UTR           complement(973..1389)
                     /note="dfnA leader region; dfnA leader region fused to
                     Pconst"
     regulatory      complement(1390..1546)
                     /label="Pconst"
                     /note="Pconst"
                     /regulatory_class="promoter"
     CDS             1997..2728
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     misc_feature    2825..3594
                     /note="LoaP Region Homology; downstream loaP homology for
                     integration into chromosome"
     gene            2825..2855
                     /pseudo=""
                     /gene="loaP"
                     /label="loaP"
                     /note="3'"
     gene            complement(3199..3594)
                     /pseudo=""
                     /locus_tag="RBA_022100"
                     /label="RBA_022100"
     primer_bind     complement(3638..3654)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3662..3678)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3686..3716)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3731..3752)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4040..4628)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4802..5659)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5660..5764)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgatgg aaggcgccgc tcacatttaa
      421 aggaaggtac attctcacgc cggctttttc aaagacgggg cccgcttttt caatgtcttg
      481 tttcgttccg gcgatcacaa tttgcgaagg tgaattgtgg ttagcgatat caatgccggt
      541 cagatgataa tcggatagaa cctcttttac ttgttcagcg gtaaacccca aaaccgccgc
      601 catgcctccg ggagctgcct tgctcatgag ctcccctctt tttttcacaa gctgcaaacc
      661 ggtctcaaaa tcaaagcagc cggccgcaaa taacgcatta tattctccta aactgtgccc
      721 tgcggcgtaa tcaggctctc tgccggactc tttcattttc tttaaataac tgagtgcact
      781 caccgtatat aacgccggct gggtaaattg cgttttcccc agctgatgat tcggatcctc
      841 cagacacaat tcttctatag agtagcctaa tatgtcatca gcctgacgtg tcagatcttg
      901 aaattcatca aacagtccgg ctcccattcc tttaaattga gaaccctgcc ccgggaacac
      961 aaacgtaagc atcttatcct catcctttcc gcatcgccaa ttttgtatat cacatgtgga
     1021 tctccataca aaaacgtctc ttatttcata tattgactgg tgtaaacacc ggcataaaaa
     1081 taaaaacaca aacgcgtttg cgattcagca caggtttaaa aatcgcaaac gcattgtgtt
     1141 ttccttttct aacataattt atataaagac atcttgcttg tttactgaat gtttcgcatg
     1201 acaagttgca gtgccaatat gcccgaaaac agccatgacg gcacacttct cccttatcat
     1261 gtatatgtag atatatacac tacaatcatt tttataatcc ctatttcctc ttcaacgtgc
     1321 cgaaacagcg attgaaattt aataggtgct ttacatcaat ttaataaatt taacaaatag
     1381 ttgctattta cacacattat gccacacctt gtagataaag tcaacaactt tttcgaaaat
     1441 ttttcaggaa ttttagcaga ggttgttctg gatgtagaac aaaacatctt tccgctcttg
     1501 tgctgtaagg atatctttct tggaagctag gtaggcaagg gctacgtcct ttaactctgg
     1561 caaccctcaa aattgaatga gacatgctac acctccggat aataaatata tataaacgta
     1621 tatagatttc ataaagtcta acacactaga cttatttact tcgtaattaa gtcgttaaac
     1681 cgtgtgctct acgaccaaaa ctataaaacc tttaagaact ttcttttttt acaagaaaaa
     1741 agaaattaga taaatctctc atatctttta ttcaataatc gcatccgatt gcagtataaa
     1801 tttaacgatc actcatcatg ttcatattta tcagagctcg tgctataatt atactaattt
     1861 tataaggagg aaaaaatatg ggcattttta gtatttttgt aatcagcaca gttcattatc
     1921 aaccaaacaa aaaataagtg gttataatga atcgttaata agcaaaattc atataaccaa
     1981 attaaagagg gttataatga acgagaaaaa tataaaacac agtcaaaact ttattacttc
     2041 aaaacataat atagataaaa taatgacaaa tataagatta aatgaacatg ataatatctt
     2101 tgaaatcggc tcaggaaaag gccattttac ccttgaatta gtaaagaggt gtaatttcgt
     2161 aactgccatt gaaatagacc ataaattatg caaaactaca gaaaataaac ttgttgatca
     2221 cgataatttc caagttttaa acaaggatat attgcagttt aaatttccta aaaaccaatc
     2281 ctataaaata tatggtaata taccttataa cataagtacg gatataatac gcaaaattgt
     2341 ttttgatagt atagctaatg agatttattt aatcgtggaa tacgggtttg ctaaaagatt
     2401 attaaataca aaacgctcat tggcattact tttaatggca gaagttgata tttctatatt
     2461 aagtatggtt ccaagagaat attttcatcc taaacctaaa gtgaatagct cacttatcag
     2521 attaagtaga aaaaaatcaa gaatatcaca caaagataaa caaaagtata attatttcgt
     2581 tatgaaatgg gttaacaaag aatacaagaa aatatttaca aaaaatcaat ttaacaattc
     2641 cttaaaacat gcaggaattg acgatttaaa caatattagc tttgaacaat tcttatctct
     2701 tttcaatagc tataaattat ttaataagta agttaaggga tgcataaact gcatccctta
     2761 acttgttttt cgtgtgccta ttttttgtga atcgatgata agctgtcaaa catgagaatt
     2821 aattcgtcgg aattgaagtg ctctccaaac cctagcatat caaaaaaagc atcaacagca
     2881 tgtgccgttg atgctttttt tatataaaca gacgaaagcc cgacaccaaa ataaccccgc
     2941 tcaccaccgt cacggtcaga ctttttgttt tcaaggcata gttgacctga agccgggctg
     3001 cgtccatgaa gcaaaatcac atttagggga agatacattt ttatcaaaag cggtgaattg
     3061 acggttgagc ttcatgacga aaagcatgtg ctgacttcgg gcgatgccgc acagttattt
     3121 taattccacg gatgaaccgg tgacatattt tatgctggtc tattatcctg aaccggctta
     3181 aacgcacatc aggcttcttc agacagaagc ctgatggacc gcaacccatt cttgtatgca
     3241 ttgaatcagt ttctggacag aaggtgtctg cgtctccttt gcatgagctg tcaaaatgcc
     3301 gagctgcctg gagacatacc ggtcgaaccg tttgatgagc acatcacaat cattcaccaa
     3361 aagctgcggc aagatgctga tccccagatt gttccgcacc attgcaataa tcgcttcatc
     3421 ccctctgatt tgataagcga cccgcggcgt aatcttttct tccttcagca ctttcagcac
     3481 atcaaaatgt gtctccggat aaggcacgat acatttgacc gactccatga catgtaacgg
     3541 aaccgtttca cattgcgcca attcatgctg cgggctcatc accgctacaa tcggctctag
     3601 agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     3661 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     3721 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     3781 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3841 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5401 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5761 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     5821 taacctataa aaataggcgt atcacgaggc cctttcgtc
//
