LOCUS       V005334                 5477 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005334
VERSION     V005334
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Goodson JR, Klupt SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 5477)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5477)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    396..1005
                     /note="loaP region homology; loaP region homology
                     containing dfnA and its leader region"
     regulatory      complement(1006..1164)
                     /label="Pconst"
                     /note="Pconst"
                     /regulatory_class="promoter"
     CDS             1615..2346
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     misc_feature    2443..3212
                     /note="LoaP Region Homology; homology region downstream of
                     loaP"
     gene            2443..2473
                     /pseudo=""
                     /gene="loaP"
                     /label="loaP"
                     /note="3'; fragment of loaP left after marker replacement"
     primer_bind     complement(3256..3272)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3280..3296)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3304..3334)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3349..3370)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3658..4246)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4420..5277)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5278..5382)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgatgg aaggcgccgc tcacatttaa
      421 aggaaggtac attctcacgc cggctttttc aaagacgggg cccgcttttt caatgtcttg
      481 tttcgttccg gcgatcacaa tttgcgaagg tgaattgtgg ttagcgatat caatgccggt
      541 cagatgataa tcggatagaa cctcttttac ttgttcagcg gtaaacccca aaaccgccgc
      601 catgcctccg ggagctgcct tgctcatgag ctcccctctt tttttcacaa gctgcaaacc
      661 ggtctcaaaa tcaaagcagc cggccgcaaa taacgcatta tattctccta aactgtgccc
      721 tgcggcgtaa tcaggctctc tgccggactc tttcattttc tttaaataac tgagtgcact
      781 caccgtatat aacgccggct gggtaaattg cgttttcccc agctgatgat tcggatcctc
      841 cagacacaat tcttctatag agtagcctaa tatgtcatca gcctgacgtg tcagatcttg
      901 aaattcatca aacagtccgg ctcccattcc tttaaattga gaaccctgcc ccgggaacac
      961 aaacgtaagc atcttatcct catcctttcc gcatcgccaa ttttcgcaca cacattatgc
     1021 cacaccttgt agataaagtc aacaactttt tcgaaaattt ttcaggaatt ttagcagagg
     1081 ttgttctgga tgtagaacaa aacatctttc cgctcttgtg ctgtaaggat atctttcttg
     1141 gaagctaggt aggcaagggc tacgtccttt aactctggca accctcaaaa ttgaatgaga
     1201 catgctacac ctccggataa taaatatata taaacgtata tagatttcat aaagtctaac
     1261 acactagact tatttacttc gtaattaagt cgttaaaccg tgtgctctac gaccaaaact
     1321 ataaaacctt taagaacttt ctttttttac aagaaaaaag aaattagata aatctctcat
     1381 atcttttatt caataatcgc atccgattgc agtataaatt taacgatcac tcatcatgtt
     1441 catatttatc agagctcgtg ctataattat actaatttta taaggaggaa aaaatatggg
     1501 catttttagt atttttgtaa tcagcacagt tcattatcaa ccaaacaaaa aataagtggt
     1561 tataatgaat cgttaataag caaaattcat ataaccaaat taaagagggt tataatgaac
     1621 gagaaaaata taaaacacag tcaaaacttt attacttcaa aacataatat agataaaata
     1681 atgacaaata taagattaaa tgaacatgat aatatctttg aaatcggctc aggaaaaggc
     1741 cattttaccc ttgaattagt aaagaggtgt aatttcgtaa ctgccattga aatagaccat
     1801 aaattatgca aaactacaga aaataaactt gttgatcacg ataatttcca agttttaaac
     1861 aaggatatat tgcagtttaa atttcctaaa aaccaatcct ataaaatata tggtaatata
     1921 ccttataaca taagtacgga tataatacgc aaaattgttt ttgatagtat agctaatgag
     1981 atttatttaa tcgtggaata cgggtttgct aaaagattat taaatacaaa acgctcattg
     2041 gcattacttt taatggcaga agttgatatt tctatattaa gtatggttcc aagagaatat
     2101 tttcatccta aacctaaagt gaatagctca cttatcagat taagtagaaa aaaatcaaga
     2161 atatcacaca aagataaaca aaagtataat tatttcgtta tgaaatgggt taacaaagaa
     2221 tacaagaaaa tatttacaaa aaatcaattt aacaattcct taaaacatgc aggaattgac
     2281 gatttaaaca atattagctt tgaacaattc ttatctcttt tcaatagcta taaattattt
     2341 aataagtaag ttaagggatg cataaactgc atcccttaac ttgtttttcg tgtgcctatt
     2401 ttttgtgaat cgatgataag ctgtcaaaca tgagaattaa ttcgtcggaa ttgaagtgct
     2461 ctccaaaccc tagcatatca aaaaaagcat caacagcatg tgccgttgat gcttttttta
     2521 tataaacaga cgaaagcccg acaccaaaat aaccccgctc accaccgtca cggtcagact
     2581 ttttgttttc aaggcatagt tgacctgaag ccgggctgcg tccatgaagc aaaatcacat
     2641 ttaggggaag atacattttt atcaaaagcg gtgaattgac ggttgagctt catgacgaaa
     2701 agcatgtgct gacttcgggc gatgccgcac agttatttta attccacgga tgaaccggtg
     2761 acatatttta tgctggtcta ttatcctgaa ccggcttaaa cgcacatcag gcttcttcag
     2821 acagaagcct gatggaccgc aacccattct tgtatgcatt gaatcagttt ctggacagaa
     2881 ggtgtctgcg tctcctttgc atgagctgtc aaaatgccga gctgcctgga gacataccgg
     2941 tcgaaccgtt tgatgagcac atcacaatca ttcaccaaaa gctgcggcaa gatgctgatc
     3001 cccagattgt tccgcaccat tgcaataatc gcttcatccc ctctgatttg ataagcgacc
     3061 cgcggcgtaa tcttttcttc cttcagcact ttcagcacat caaaatgtgt ctccggataa
     3121 ggcacgatac atttgaccga ctccatgaca tgtaacggaa ccgtttcaca ttgcgccaat
     3181 tcatgctgcg ggctcatcac cgctacaatc ggctctagag tcgacctgca ggcatgcaag
     3241 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3301 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3361 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3421 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3481 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4081 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4381 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4441 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4501 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4561 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4621 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4681 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4741 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4801 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4861 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4921 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4981 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5041 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5101 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5161 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5221 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5281 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5341 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5401 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     5461 cacgaggccc tttcgtc
//
