LOCUS       V005335                10626 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005335
VERSION     V005335
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Goodson JR, Klupt SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 10626)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10626)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..107
                     /label="AmpR promoter"
     CDS             108..965
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1139..1727
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1977..2756)
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     misc_feature    3865..4865
                     /pseudo=""
                     /note="amyE; amyE back; amyE homology for integration into
                     B. amyloliquefaciens"
     CDS             complement(5239..5886)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      6196..6337
                     /note="PdfnA; promoter region of dfnA from B.
                     amyloliquefaciens FZB42"
                     /regulatory_class="promoter"
     misc_feature    6345..6622
                     /note="dfnA leader region (truncated); dfnA leader region
                     missing basepairs +76-198"
     CDS             6638..7354
                     /label="mVenus"
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     CDS             complement(7471..8001)
                     /codon_start=1
                     /gene="loaP"
                     /product="LoaP"
                     /label="loaP"
                     /note="LoaP; LoaP NusG-like antiterminator protein"
                     /protein_id="APU90147.1"
                     /translation="MKWYALFVESGKEETVQKFLRLQFDEQALYSIIPKKKVTERKAGI
                     KYEALKKMFPGYVLFKTKMTERTFHKIKELPISCRIVNNGAYYSKERKTYFTTIKDEEI
                     LPIIRLIGEGDTVDYSKVYIENSKVTVASGPLKGMEGIIKKIDKRKRRAKICLSFMGLD
                     KMVNVGIEVLSKP"
     gene            complement(7471..8001)
                     /gene="loaP"
                     /label="loaP"
     CDS             complement(8030..8089)
                     /codon_start=1
                     /gene="mini-xylA"
                     /product="XylA fragment"
                     /label="mini-xylA"
                     /note="mini xylA ORF"
                     /protein_id="APU90148.1"
                     /translation="MAQSHSSSVNYFVSVNKVV"
     gene            complement(8030..8089)
                     /gene="mini-xylA"
                     /label="mini-xylA"
                     /note="xylA"
     regulatory      complement(8159..8238)
                     /note="PxylA; PxylA Xylose-Inducible Promoter"
                     /regulatory_class="promoter"
     CDS             8450..9502
                     /codon_start=1
                     /gene="xylR"
                     /product="XylR Xylose Repressor protein"
                     /label="xylR"
                     /note="XylR"
                     /protein_id="APU90149.1"
                     /translation="MTGLNKSTVSSQVNTLMKENLVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSIGIDVGVDYISGILTDLEGTIILDQHHHLESNSPEITKDILIDMIHHFITRMPQSP
                     YGLIGIGICVPGLIDKNQKIVFTPNSNWRDIDLKSFIQEKFNVPVFIENEANAGAYGEK
                     VFGAAKNHNNIIYASISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKVSYQDIIDLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAIILRNSIIESHPMVLNSIRSEVSSRVYPQLGNSYELLPSSLGKNAPALGMSSIV
                     IEHFLDIVKM"
     gene            8450..9502
                     /gene="xylR"
                     /label="xylR"
     misc_feature    9510..10409
                     /pseudo=""
                     /note="amyE; amyE front; amyE homology for integration into
                     B. amyloliquefaciens"
ORIGIN
        1 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
       61 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      121 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      181 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      241 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      301 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
      361 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      421 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      481 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      541 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      601 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
      661 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
      721 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
      781 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
      841 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
      901 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
      961 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1021 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1081 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1141 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1201 cggtggtttg tttgccggat caagagctat caactctttt tccgaaggta actggcttca
     1261 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1321 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1381 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1441 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1501 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1561 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1621 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1681 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1741 cggcccgacc tcgagctgga tacttcccgt ccgccagggg gacatgccgg cgatgctgaa
     1801 ggtcgcgcgc attcccgatg aagaggccgg ttaccgcctg tttgaggata tagtaatctt
     1861 tctaaatagc tttggattgg aggagtatgg ccactaatac taagttcagc taataaaaaa
     1921 atttgctaaa gaactccaga aaagtaagca cctgttattg caataaaatt agcctaattg
     1981 agagaagttt ctatagaatt tttcatatac ttaacgagtg ctttcacctt tgaatatagt
     2041 ccttcccact tatcatcaca ctctccccga tagccttttc tagctatatc cagtaaagtt
     2101 acatgctctt taggtaaaag aggtatagcc cattctgcag cgacatcttt cgaggtaatt
     2161 tcaccagtag tcactgtttg ccacattcga gctagggtta aaattacatt acgctcatca
     2221 ccttttatcc cctcaattag ttctggcaaa gaatccttaa ttgctcttcg aatatctgtc
     2281 aaaggtacgg agacaagtat acttgaagaa tcaggaccaa atagagaaat actattcttt
     2341 cttgcttgtg ctaaaacaat agccaaatca ggatcatagc ttggttcctg aatttgtcca
     2401 ttctcaaatt cacccctgag ccactcaccg tatataaatt ctctttttgg aggatattgc
     2461 caagggacaa cttcactcct atttataacc gtaacttcaa gtggtctaac agaatccgta
     2521 tttccaatct ttcctgatat agtcattagt ctttctgtta gtttttttcg agttaattga
     2581 ggtaaactat gattcacgac gactagaaca tctacatcgc tgttaatgcg taaaccacca
     2641 tttactgctg aaccaaatag atatactcca actattgaac ttccaaataa atcttttacg
     2701 atttttaatg tttgaatcgc ttgatttggt atttttccgt taatcaaatt gctcatgatt
     2761 tcacctcgtt gattatgttc atataaagtt tatattgata ctcaatttac ttaccctaga
     2821 ttggacatat acttaaatta ctgttcaata aagctgaccg ttagcgttta agtacatcct
     2881 ttcacaattt gtctacagat taataattat tctttattat acagatcgat cctctagacc
     2941 taggccttaa gatctgatca tatgcatccg cgggcccggg ttaacgcgta atccatggat
     3001 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     3061 ccggtgccct gaatgaactg cagaaagagc tggtagttgg cgcactgttc gaagaactgc
     3121 cgatgtccag taagattctt actatgctgg ttgaaccgga tgctggtaaa gctacttggg
     3181 ttgctgcttc tacttatggt accgatacaa ctactggtga ggaagttaaa ggagctctta
     3241 aagaaatcca cttcagtagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca
     3301 gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc
     3361 ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc
     3421 gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
     3481 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
     3541 gcctcgcgtc gcgactaaga aaatgccgtc aaatccgctc gccatgactt cactaacgat
     3601 gcctttgaaa atcttcaagt tcttttctac taattcaagg cgtgtctcac caggtttttg
     3661 gtttgctccg gcgcaaatgc agacaatatc agcatccttg cagggtatgt ttctctttga
     3721 tgtctttttg tttgtgaagt atttcacatt tatattgtgc aacacttcac aaacttttgc
     3781 aagagaaaag ttttgtctga tttatgaaca aaaaagaaac catcattgat ggtttctttc
     3841 ggtaagtccc gtctagcctt gcccttaatg cggaagataa ccattcaaac cggagttgtt
     3901 atacaaacca ttctgcacat aatcaaagcc gggctggttc tgtccgggca cttgggcgct
     3961 gccattgtta aaaatcactt tggcattggc cgtatccgta ttctcaggca gcgtcagcgt
     4021 gtacattcca tttgcattct tagtcattgc tttccccggc cacgatccgg ttaattctat
     4081 ggccctgcct ccatcatgtt tgtaaatata agcatttacc tggccccaat ggtccggatt
     4141 ttgatagcca atgatgttgg tttgggacgg gtcttttttg acaaatgtgt attcttgggt
     4201 tctcgctgca ccctcgccgt tcgttccggt taatttgatg ttgtatgtcg tgccgattgg
     4261 atctcctttc ccgatcgtta gtcggtctcc atccttaaat gctgtctgct gcccattatt
     4321 gatttggtaa acggcttttg ttgttttcgc atttgcacgc agggtgaccg tcagctgatc
     4381 attgaaagaa tggactgccc ccgtttggta attctcaaga aagacatgag gcgcatttcc
     4441 aatatcatca ggataaagaa cagccgcgga tctggcattg atcgtacctg tcagtttgcc
     4501 gttcgctact tgaaatgaac cggcgccggc cctattatca tacctgccgt caggtaattt
     4561 cgctgaagta ttgatggtga cagaagacga tcccgcattt gccagcacaa cgccttttga
     4621 gccgcgctga ttcataaaga cttggttgtt cccattcgga ttcgagagtt cctcgggctg
     4681 cccggccatt acattgtgaa atgtattgac cgcagtgatc gcctgatctt taaataaggc
     4741 gctcccgcga tctcctattt gactttttcc gggaaatctt acaccatttc ctccgccctc
     4801 aggtctggaa aagaaaagag gcgtgcttcc tgagcgggaa ccaatcactg cccagcctaa
     4861 acgaacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     4921 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     4981 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     5041 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     5101 cgccggtgat gccggccacg atgcgtccgg cgtagaggat ctggagctgt aatataaaaa
     5161 ccttcttcaa ctaacggggc aggttagtga cattagaaaa ccgactgtaa aaagtacagt
     5221 cggcattatc tcatattata aaagccagtc attaggccta tctgacaatt cctgaataga
     5281 gttcataaac aatcctgcat gataaccatc acaaacagaa tgatgtacct gtaaagatag
     5341 cggtaaatat attgaattac ctttattaat gaattttcct gctgtaataa tgggtagaag
     5401 gtaattacta ttattattga tatttaagtt aaacccagta aatgaagtcc atggaataat
     5461 agaaagagaa aaagcatttt caggtatagg tgttttggga aacaatttcc ccgaaccatt
     5521 atatttctct acatcagaaa ggtataaatc ataaaactct ttgaagtcat tctttacagg
     5581 agtccaaata ccagagaatg ttttagatac accatcaaaa attgtataaa gtggctctaa
     5641 cttatcccaa taacctaact ctccgtcgct attgtaacca gttctaaaag ctgtatttga
     5701 gtttatcacc cttgtcacta agaaaataaa tgcagggtaa aatttatatc cttcttgttt
     5761 tatgtttcgg tataaaacac taatatcaat ttctgtggtt atactaaaag tcgtttgttg
     5821 gttcaaataa tgattaaata tctcttttct cttccaattg tctaaatcaa ttttattaaa
     5881 gttcatttga tatgcctcct aaatttttat ctaaagtgaa tttaggaggc ttacttgtct
     5941 gctttcttca ttagaatcaa tcctttttta aaagtcaata ttactgtaac ataaatatat
     6001 attttaaaaa tatcccactt tatccaattt tcgtttgttg aactaatggg tgctttagtt
     6061 gaagaataaa gaccacatta aaaaatgtgg tcttttgtgt ttttttaaag gatttgagcg
     6121 tagcgaaaaa tccttttctt tcttatcttg ataataaggg taactattgc cgatgataag
     6181 ctgtcaaaca tgagggacat gaatagggtt cagggaatta tctgacattt atcccgaaac
     6241 aattatgccg actgtccacc gaacataaaa ctgtggaata aatgtcagaa aataacgaaa
     6301 taaaaccttg acattattat ctataaattg atattattct ctttaaatag caactatttg
     6361 ttaaatttat taaattgatg taaagcacct attaaatttc aatcgctgtt tcggcacgtc
     6421 attcagtaaa caagcaagat gtctttatat aaattatgtt agaaaaggaa aacacaatgc
     6481 gtttgcgatt tttaaacctg tgctgaatcg caaacgcgtt tgtgttttta tttttatgcc
     6541 ggtgtttaca ccagtcaata tatgaaataa gagacgtttt tgtatggaga tccacatgtg
     6601 atatacaaaa ttggcgatgc ggaaggagga aagtcacatt atgagcaaag gtgaagaact
     6661 gttcaccggc gttgtgccaa ttctggttga gctggatggt gacgtgaatg gccacaaatt
     6721 ttccgtgtct ggtgaaggcg agtgtgatgc tacttatggc aaactgactc tgaaactgat
     6781 ctgtaccacc ggcaaactgc ctgttccgtg gccaactctg gtcactactc tgggttacgg
     6841 cctgatgtgt tttgcgcgtt acccggatca catgaaacag catgacttct tcaaatctgc
     6901 catgccggaa ggctatgtcc aagaacgtac gatctttttc aaggacgacg gcaactataa
     6961 aacccgtgcc gaagttaaat tcgagggtga caccctggtt aaccgcatcg aactgaaagg
     7021 cattgacttc aaagaggacg gcaacattct gggtcacaag ctggaataca actacaactc
     7081 ccacaacgtt tacattactg ctgacaagca gaaaaacggc atcaaagcaa acttcaagat
     7141 ccgtcacaac attgaagatg gtggcgtaca gctggcagat cactaccagc agaacactcc
     7201 aatcggtgat ggcccagtac tgctgccaga taaccattac ctgtcctacc agagcaaact
     7261 gtctaaagac ccgaacgaaa aacgtgacca catggtactg ctggaatttg ttaccgcggc
     7321 aggcattacc cacggtatgg acgaactgta taaataagct agcaaaaacc ccgcccctga
     7381 cagggcgggg ttttttttga attccgatca gaccagtttt taacaaaaaa agcatcaacg
     7441 gcacatgctg ttgatgcttt ttttgatatg ctagggtttg gagagcactt caattccgac
     7501 gttgaccatt ttgtccagtc ccataaaaga caggcagatt ttggcgcgtc ttttcctttt
     7561 atcgatcttt ttaataatgc cttccattcc tttaagaggc cctgaggcaa cggttacttt
     7621 tgaattttca atatagactt ttgaataatc tacagtgtct ccttctccga tcagcctgat
     7681 aatagggagg atttcttcat ccttaattgt cgtgaaatag gttttacgct ctttggaata
     7741 atatgcgccg ttattcacga ttctgcatga aatggggagt tctttgattt tatgaaatgt
     7801 ccgctctgtc atcttcgttt taaacagcac atatcccgga aacatctttt tgagtgcctc
     7861 gtatttgatt ccggcttttc gttctgtgac ttttttcttg ggtatgattg agtagagagc
     7921 ctgctcatca aactgcagac gcagaaattt ttgaactgtt tcttcttttc cggactctac
     7981 aaaaagtgcg taccatttca tgactgggtc ctccttctgt cgacttaatt taaaccactt
     8041 tgttaacgct tacaaaatag ttaactgaac tagaatgaga ttgagccatg ttatttcctc
     8101 cttaaaaata aattcaaata cagatgcatt ttatgtcata ttgtaagtaa gttgcacatt
     8161 agtttgttgc ccaaacaaac taacttattt taatcttaaa taacctcatc agtattttca
     8221 atattttttt tagtttttca tgaacacatt agatataata aaggtaagat tcgctatgta
     8281 ctaaatctca tactgaacac aaaaaatttt aacttaattt aaaggttata caaatggagt
     8341 ggatgaagtg gatatcgcag atcaaacctt tgtcaaaaaa gtaaatcaaa agttattatt
     8401 aaaagaaata cttaagaatt cacctatttc aagagcaaaa ttatctgaaa tgactggatt
     8461 aaataaatca actgtttcat cacaggtaaa cacgctgatg aaagaaaatc ttgtatttga
     8521 aataggtcaa ggacaatcaa gtggcggaag aagacctgtc atgcttgttt ttaataaaaa
     8581 ggcaggatac tccataggaa tagatgttgg tgtggattat attagtggca ttttaacaga
     8641 ccttgaagga acgatcattc ttgatcaaca tcaccattta gaatcgaatt ctccagaaat
     8701 aactaaagac attttaattg atatgattca tcactttatt acgcgtatgc cacaatctcc
     8761 gtacgggctt atcggtatag gcatatgcgt gcctggacta attgataaaa atcaaaaaat
     8821 tgttttcact ccgaactcca actggagaga tattgactta aaatctttca tacaagagaa
     8881 gttcaatgtg cctgttttta ttgaaaatga ggcaaatgct ggcgcgtatg gagaaaaagt
     8941 atttggtgct gcaaaaaatc acaataacat tatttatgct agtatcagta caggtatagg
     9001 gatcggtgtt attatcaaca atcatttata tagaggggta agcggattct ctggagaaat
     9061 gggacatatg acaatagact ttaatggtcc taaatgcagt tgcggaaacc gaggttgctg
     9121 ggaattgtat gcttcagaga aggctttatt aaaatctctt cagactaaag agaaaaaagt
     9181 gtcctatcaa gatatcatag acctcgccca tctgaatgat atcggcacct taaatgcatt
     9241 acagaatttc ggattctatt taggaattgg ccttactaat attctaaata cattcaatcc
     9301 acaagccatc attttaagaa acagcataat tgaatcacat cctatggttt taaattcaat
     9361 tagaagtgaa gtgtcgtcca gggtttatcc ccaattaggc aatagctatg aattattacc
     9421 atcttcctta ggaaagaatg caccggcatt aggaatgtct tccattgtta ttgaacattt
     9481 tctagatatc gttaaaatgt aaaagcttcg tccgcagaca cgtcagatgc ataatgggag
     9541 atattcgaca cactcagatt acggttcttt aaagcggatc tgatggaatg cccgtagtta
     9601 gaagccgtca cattcatata attcgcataa gcagtatctc tggacgcgct gtcttgcagg
     9661 atttctccgt attggaactc cgctgatgta tttgtgatat tcggccaaaa ttggctgccg
     9721 taattcccgt catccggaag ctctatatgc ttggcggcat catagcggaa cccgtctgct
     9781 ccgtcattca atgctctttc caagaaacgt ttcagataga cctgcacctc agtattctgt
     9841 gtattccaat catacagccc gagcaatgaa ttttgagtga cgtcccatcg atccgaccaa
     9901 tttttaattt gtgtgtttcc atgggtccag tttggaatac gcttaatctc gtcagaaatc
     9961 gcgccataat cgctggtagt atgattgata accgcatcga caatgacttt tacgccatat
    10021 ttttccgccg ctgcacacat gtccttaaat tcttgttcag tgcctaagta acggttgccg
    10081 atttggtacg atgtcggctg atacagccag taccagttcc tcatgctttt atctccttgg
    10141 ttcccttcct ttacttggtt aatcggagac gtctgaatgg ctgcatagcc cgcatcacga
    10201 atatctttca tattttgtgt taacgtattg aacgaccaat tccatgcatg aaggatggtc
    10261 ccgtttttga ccgatgacgc ggtcacctta ttcgatttgt ttgcagtttc agcgtttgca
    10321 gccgccgggc ctgacaaaac caaatgaaac agcaataaaa atccggcgaa taacggcagt
    10381 aaagaggttt tgaatcgttt ttcaaacatt cttgacactc cttatttgat tttttgaaga
    10441 cttacttcgg agtcaaaaat ccctcttact tcattcttcc gcttcctcct ttcaaaccga
    10501 tgtgaagact ggagaatttt gttaattctt gaagacgaaa gggcctcgtg atacgcctat
    10561 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
    10621 gaaatg
//
