LOCUS       V005336                10777 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005336
VERSION     V005336
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10777)
  AUTHORS   Goodson JR, Klupt SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 10777)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10777)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..107
                     /label="AmpR promoter"
     CDS             108..965
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1139..1727
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1977..2756)
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     misc_feature    3865..4865
                     /pseudo=""
                     /note="amyE; amyE back; amyE homology for integration into
                     B. amyloliquefaciens"
     CDS             complement(5239..5886)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      6196..6337
                     /note="PdfnA; promoter region of dfnA from B.
                     amyloliquefaciens FZB42"
                     /regulatory_class="promoter"
     misc_feature    6345..6622
                     /note="dfnA leader region (truncated); dfnA leader region
                     missing basepairs +76-198"
     terminator      6622..6669
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     regulatory      6671..6716
                     /note="lambda tR2 terminator; transcription terminator tR2
                     from lambda phage"
                     /regulatory_class="terminator"
     terminator      complement(6720..6763)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             6789..7505
                     /label="mVenus"
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     CDS             complement(7622..8152)
                     /codon_start=1
                     /gene="loaP"
                     /product="LoaP"
                     /label="loaP"
                     /note="LoaP; LoaP NusG-like antiterminator protein"
                     /protein_id="APU90176.1"
                     /translation="MKWYALFVESGKEETVQKFLRLQFDEQALYSIIPKKKVTERKAGI
                     KYEALKKMFPGYVLFKTKMTERTFHKIKELPISCRIVNNGAYYSKERKTYFTTIKDEEI
                     LPIIRLIGEGDTVDYSKVYIENSKVTVASGPLKGMEGIIKKIDKRKRRAKICLSFMGLD
                     KMVNVGIEVLSKP"
     gene            complement(7622..8152)
                     /gene="loaP"
                     /label="loaP"
     CDS             complement(8181..8240)
                     /codon_start=1
                     /gene="mini-xylA"
                     /product="XylA fragment"
                     /label="mini-xylA"
                     /note="mini xylA ORF"
                     /protein_id="APU90177.1"
                     /translation="MAQSHSSSVNYFVSVNKVV"
     gene            complement(8181..8240)
                     /gene="mini-xylA"
                     /label="mini-xylA"
                     /note="xylA"
     regulatory      complement(8310..8389)
                     /note="PxylA; PxylA Xylose-Inducible Promoter"
                     /regulatory_class="promoter"
     CDS             8601..9653
                     /codon_start=1
                     /gene="xylR"
                     /product="XylR Xylose Repressor protein"
                     /label="xylR"
                     /note="XylR"
                     /protein_id="APU90178.1"
                     /translation="MTGLNKSTVSSQVNTLMKENLVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSIGIDVGVDYISGILTDLEGTIILDQHHHLESNSPEITKDILIDMIHHFITRMPQSP
                     YGLIGIGICVPGLIDKNQKIVFTPNSNWRDIDLKSFIQEKFNVPVFIENEANAGAYGEK
                     VFGAAKNHNNIIYASISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKVSYQDIIDLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAIILRNSIIESHPMVLNSIRSEVSSRVYPQLGNSYELLPSSLGKNAPALGMSSIV
                     IEHFLDIVKM"
     gene            8601..9653
                     /gene="xylR"
                     /label="xylR"
     misc_feature    9661..10560
                     /pseudo=""
                     /note="amyE; amyE front; amyE homology for integration into
                     B. amyloliquefaciens"
ORIGIN
        1 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
       61 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      121 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      181 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      241 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      301 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
      361 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      421 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      481 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      541 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      601 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
      661 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
      721 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
      781 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
      841 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
      901 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
      961 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1021 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1081 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1141 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1201 cggtggtttg tttgccggat caagagctat caactctttt tccgaaggta actggcttca
     1261 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1321 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1381 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1441 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1501 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1561 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1621 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1681 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1741 cggcccgacc tcgagctgga tacttcccgt ccgccagggg gacatgccgg cgatgctgaa
     1801 ggtcgcgcgc attcccgatg aagaggccgg ttaccgcctg tttgaggata tagtaatctt
     1861 tctaaatagc tttggattgg aggagtatgg ccactaatac taagttcagc taataaaaaa
     1921 atttgctaaa gaactccaga aaagtaagca cctgttattg caataaaatt agcctaattg
     1981 agagaagttt ctatagaatt tttcatatac ttaacgagtg ctttcacctt tgaatatagt
     2041 ccttcccact tatcatcaca ctctccccga tagccttttc tagctatatc cagtaaagtt
     2101 acatgctctt taggtaaaag aggtatagcc cattctgcag cgacatcttt cgaggtaatt
     2161 tcaccagtag tcactgtttg ccacattcga gctagggtta aaattacatt acgctcatca
     2221 ccttttatcc cctcaattag ttctggcaaa gaatccttaa ttgctcttcg aatatctgtc
     2281 aaaggtacgg agacaagtat acttgaagaa tcaggaccaa atagagaaat actattcttt
     2341 cttgcttgtg ctaaaacaat agccaaatca ggatcatagc ttggttcctg aatttgtcca
     2401 ttctcaaatt cacccctgag ccactcaccg tatataaatt ctctttttgg aggatattgc
     2461 caagggacaa cttcactcct atttataacc gtaacttcaa gtggtctaac agaatccgta
     2521 tttccaatct ttcctgatat agtcattagt ctttctgtta gtttttttcg agttaattga
     2581 ggtaaactat gattcacgac gactagaaca tctacatcgc tgttaatgcg taaaccacca
     2641 tttactgctg aaccaaatag atatactcca actattgaac ttccaaataa atcttttacg
     2701 atttttaatg tttgaatcgc ttgatttggt atttttccgt taatcaaatt gctcatgatt
     2761 tcacctcgtt gattatgttc atataaagtt tatattgata ctcaatttac ttaccctaga
     2821 ttggacatat acttaaatta ctgttcaata aagctgaccg ttagcgttta agtacatcct
     2881 ttcacaattt gtctacagat taataattat tctttattat acagatcgat cctctagacc
     2941 taggccttaa gatctgatca tatgcatccg cgggcccggg ttaacgcgta atccatggat
     3001 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     3061 ccggtgccct gaatgaactg cagaaagagc tggtagttgg cgcactgttc gaagaactgc
     3121 cgatgtccag taagattctt actatgctgg ttgaaccgga tgctggtaaa gctacttggg
     3181 ttgctgcttc tacttatggt accgatacaa ctactggtga ggaagttaaa ggagctctta
     3241 aagaaatcca cttcagtagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca
     3301 gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc
     3361 ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc
     3421 gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
     3481 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
     3541 gcctcgcgtc gcgactaaga aaatgccgtc aaatccgctc gccatgactt cactaacgat
     3601 gcctttgaaa atcttcaagt tcttttctac taattcaagg cgtgtctcac caggtttttg
     3661 gtttgctccg gcgcaaatgc agacaatatc agcatccttg cagggtatgt ttctctttga
     3721 tgtctttttg tttgtgaagt atttcacatt tatattgtgc aacacttcac aaacttttgc
     3781 aagagaaaag ttttgtctga tttatgaaca aaaaagaaac catcattgat ggtttctttc
     3841 ggtaagtccc gtctagcctt gcccttaatg cggaagataa ccattcaaac cggagttgtt
     3901 atacaaacca ttctgcacat aatcaaagcc gggctggttc tgtccgggca cttgggcgct
     3961 gccattgtta aaaatcactt tggcattggc cgtatccgta ttctcaggca gcgtcagcgt
     4021 gtacattcca tttgcattct tagtcattgc tttccccggc cacgatccgg ttaattctat
     4081 ggccctgcct ccatcatgtt tgtaaatata agcatttacc tggccccaat ggtccggatt
     4141 ttgatagcca atgatgttgg tttgggacgg gtcttttttg acaaatgtgt attcttgggt
     4201 tctcgctgca ccctcgccgt tcgttccggt taatttgatg ttgtatgtcg tgccgattgg
     4261 atctcctttc ccgatcgtta gtcggtctcc atccttaaat gctgtctgct gcccattatt
     4321 gatttggtaa acggcttttg ttgttttcgc atttgcacgc agggtgaccg tcagctgatc
     4381 attgaaagaa tggactgccc ccgtttggta attctcaaga aagacatgag gcgcatttcc
     4441 aatatcatca ggataaagaa cagccgcgga tctggcattg atcgtacctg tcagtttgcc
     4501 gttcgctact tgaaatgaac cggcgccggc cctattatca tacctgccgt caggtaattt
     4561 cgctgaagta ttgatggtga cagaagacga tcccgcattt gccagcacaa cgccttttga
     4621 gccgcgctga ttcataaaga cttggttgtt cccattcgga ttcgagagtt cctcgggctg
     4681 cccggccatt acattgtgaa atgtattgac cgcagtgatc gcctgatctt taaataaggc
     4741 gctcccgcga tctcctattt gactttttcc gggaaatctt acaccatttc ctccgccctc
     4801 aggtctggaa aagaaaagag gcgtgcttcc tgagcgggaa ccaatcactg cccagcctaa
     4861 acgaacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     4921 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     4981 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     5041 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     5101 cgccggtgat gccggccacg atgcgtccgg cgtagaggat ctggagctgt aatataaaaa
     5161 ccttcttcaa ctaacggggc aggttagtga cattagaaaa ccgactgtaa aaagtacagt
     5221 cggcattatc tcatattata aaagccagtc attaggccta tctgacaatt cctgaataga
     5281 gttcataaac aatcctgcat gataaccatc acaaacagaa tgatgtacct gtaaagatag
     5341 cggtaaatat attgaattac ctttattaat gaattttcct gctgtaataa tgggtagaag
     5401 gtaattacta ttattattga tatttaagtt aaacccagta aatgaagtcc atggaataat
     5461 agaaagagaa aaagcatttt caggtatagg tgttttggga aacaatttcc ccgaaccatt
     5521 atatttctct acatcagaaa ggtataaatc ataaaactct ttgaagtcat tctttacagg
     5581 agtccaaata ccagagaatg ttttagatac accatcaaaa attgtataaa gtggctctaa
     5641 cttatcccaa taacctaact ctccgtcgct attgtaacca gttctaaaag ctgtatttga
     5701 gtttatcacc cttgtcacta agaaaataaa tgcagggtaa aatttatatc cttcttgttt
     5761 tatgtttcgg tataaaacac taatatcaat ttctgtggtt atactaaaag tcgtttgttg
     5821 gttcaaataa tgattaaata tctcttttct cttccaattg tctaaatcaa ttttattaaa
     5881 gttcatttga tatgcctcct aaatttttat ctaaagtgaa tttaggaggc ttacttgtct
     5941 gctttcttca ttagaatcaa tcctttttta aaagtcaata ttactgtaac ataaatatat
     6001 attttaaaaa tatcccactt tatccaattt tcgtttgttg aactaatggg tgctttagtt
     6061 gaagaataaa gaccacatta aaaaatgtgg tcttttgtgt ttttttaaag gatttgagcg
     6121 tagcgaaaaa tccttttctt tcttatcttg ataataaggg taactattgc cgatgataag
     6181 ctgtcaaaca tgagggacat gaatagggtt cagggaatta tctgacattt atcccgaaac
     6241 aattatgccg actgtccacc gaacataaaa ctgtggaata aatgtcagaa aataacgaaa
     6301 taaaaccttg acattattat ctataaattg atattattct ctttaaatag caactatttg
     6361 ttaaatttat taaattgatg taaagcacct attaaatttc aatcgctgtt tcggcacgtc
     6421 attcagtaaa caagcaagat gtctttatat aaattatgtt agaaaaggaa aacacaatgc
     6481 gtttgcgatt tttaaacctg tgctgaatcg caaacgcgtt tgtgttttta tttttatgcc
     6541 ggtgtttaca ccagtcaata tatgaaataa gagacgtttt tgtatggaga tccacatgtg
     6601 atatacaaaa ttggcgatgc ggtagcataa ccccttgggg cctctaaacg ggtcttgagg
     6661 ggttttttgt actgagctaa taacaggcct gctggtaatc gcaggccttt ttatttcaaa
     6721 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt taatctggct agtaaggagg
     6781 aaagtcacat tatgagcaaa ggtgaagaac tgttcaccgg cgttgtgcca attctggttg
     6841 agctggatgg tgacgtgaat ggccacaaat tttccgtgtc tggtgaaggc gagggtgatg
     6901 ctacttatgg caaactgact ctgaaactga tctgtaccac cggcaaactg cctgttccgt
     6961 ggccaactct ggtcactact ctgggttacg gcctgatgtg ttttgcgcgt tacccggatc
     7021 acatgaaaca gcatgacttc ttcaaatctg ccatgccgga aggctatgtc caagaacgta
     7081 cgatcttttt caaggacgac ggcaactata aaacccgtgc cgaagttaaa ttcgagggtg
     7141 acaccctggt taaccgcatc gaactgaaag gcattgactt caaagaggac ggcaacattc
     7201 tgggtcacaa gctggaatac aactacaact cccacaacgt ttacattact gctgacaagc
     7261 agaaaaacgg catcaaagca aacttcaaga tccgtcacaa cattgaagat ggtggcgtac
     7321 agctggcaga tcactaccag cagaacactc caatcggtga tggcccagta ctgctgccag
     7381 ataaccatta cctgtcctac cagagcaaac tgtctaaaga cccgaacgaa aaacgtgacc
     7441 acatggtact gctggaattt gttaccgcgg caggcattac ccacggtatg gacgaactgt
     7501 ataaataagc tagcaaaaac cccgcccctg acagggcggg gttttttttg aattccgatc
     7561 agaccagttt ttaacaaaaa aagcatcaac ggcacatgct gttgatgctt tttttgatat
     7621 gctagggttt ggagagcact tcaattccga cgttgaccat tttgtccagt cccataaaag
     7681 acaggcagat tttggcgcgt cttttccttt tatcgatctt tttaataatg ccttccattc
     7741 ctttaagagg ccctgaggca acggttactt ttgaattttc aatatagact tttgaataat
     7801 ctacagtgtc tccttctccg atcagcctga taatagggag gatttcttca tccttaattg
     7861 tcgtgaaata ggttttacgc tctttggaat aatatgcgcc gttattcacg attctgcatg
     7921 aaatggggag ttctttgatt ttatgaaatg tccgctctgt catcttcgtt ttaaacagca
     7981 catatcccgg aaacatcttt ttgagtgcct cgtatttgat tccggctttt cgttctgtga
     8041 cttttttctt gggtatgatt gagtagagag cctgctcatc aaactgcaga cgcagaaatt
     8101 tttgaactgt ttcttctttt ccggactcta caaaaagtgc gtaccatttc atgactgggt
     8161 cctccttctg tcgacttaat ttaaaccact ttgttaacgc ttacaaaata gttaactgaa
     8221 ctagaatgag attgagccat gttatttcct ccttaaaaat aaattcaaat acagatgcat
     8281 tttatgtcat attgtaagta agttgcacat tagtttgttg cccaaacaaa ctaacttatt
     8341 ttaatcttaa ataacctcat cagtattttc aatatttttt ttagtttttc atgaacacat
     8401 tagatataat aaaggtaaga ttcgctatgt actaaatctc atactgaaca caaaaaattt
     8461 taacttaatt taaaggttat acaaatggag tggatgaagt ggatatcgca gatcaaacct
     8521 ttgtcaaaaa agtaaatcaa aagttattat taaaagaaat acttaagaat tcacctattt
     8581 caagagcaaa attatctgaa atgactggat taaataaatc aactgtttca tcacaggtaa
     8641 acacgctgat gaaagaaaat cttgtatttg aaataggtca aggacaatca agtggcggaa
     8701 gaagacctgt catgcttgtt tttaataaaa aggcaggata ctccatagga atagatgttg
     8761 gtgtggatta tattagtggc attttaacag accttgaagg aacgatcatt cttgatcaac
     8821 atcaccattt agaatcgaat tctccagaaa taactaaaga cattttaatt gatatgattc
     8881 atcactttat tacgcgtatg ccacaatctc cgtacgggct tatcggtata ggcatatgcg
     8941 tgcctggact aattgataaa aatcaaaaaa ttgttttcac tccgaactcc aactggagag
     9001 atattgactt aaaatctttc atacaagaga agttcaatgt gcctgttttt attgaaaatg
     9061 aggcaaatgc tggcgcgtat ggagaaaaag tatttggtgc tgcaaaaaat cacaataaca
     9121 ttatttatgc tagtatcagt acaggtatag ggatcggtgt tattatcaac aatcatttat
     9181 atagaggggt aagcggattc tctggagaaa tgggacatat gacaatagac tttaatggtc
     9241 ctaaatgcag ttgcggaaac cgaggttgct gggaattgta tgcttcagag aaggctttat
     9301 taaaatctct tcagactaaa gagaaaaaag tgtcctatca agatatcata gacctcgccc
     9361 atctgaatga tatcggcacc ttaaatgcat tacagaattt cggattctat ttaggaattg
     9421 gccttactaa tattctaaat acattcaatc cacaagccat cattttaaga aacagcataa
     9481 ttgaatcaca tcctatggtt ttaaattcaa ttagaagtga agtgtcgtcc agggtttatc
     9541 cccaattagg caatagctat gaattattac catcttcctt aggaaagaat gcaccggcat
     9601 taggaatgtc ttccattgtt attgaacatt ttctagatat cgttaaaatg taaaagcttc
     9661 gtccgcagac acgtcagatg cataatggga gatattcgac acactcagat tacggttctt
     9721 taaagcggat ctgatggaat gcccgtagtt agaagccgtc acattcatat aattcgcata
     9781 agcagtatct ctggacgcgc tgtcttgcag gatttctccg tattggaact ccgctgatgt
     9841 atttgtgata ttcggccaaa attggctgcc gtaattcccg tcatccggaa gctctatatg
     9901 cttggcggca tcatagcgga acccgtctgc tccgtcattc aatgctcttt ccaagaaacg
     9961 tttcagatag acctgcacct cagtattctg tgtattccaa tcatacagcc cgagcaatga
    10021 attttgagtg acgtcccatc gatccgacca atttttaatt tgtgtgtttc catgggtcca
    10081 gtttggaata cgcttaatct cgtcagaaat cgcgccataa tcgctggtag tatgattgat
    10141 aaccgcatcg acaatgactt ttacgccata tttttccgcc gctgcacaca tgtccttaaa
    10201 ttcttgttca gtgcctaagt aacggttgcc gatttggtac gatgtcggct gatacagcca
    10261 gtaccagttc ctcatgcttt tatctccttg gttcccttcc tttacttggt taatcggaga
    10321 cgtctgaatg gctgcatagc ccgcatcacg aatatctttc atattttgtg ttaacgtatt
    10381 gaacgaccaa ttccatgcat gaaggatggt cccgtttttg accgatgacg cggtcacctt
    10441 attcgatttg tttgcagttt cagcgtttgc agccgccggg cctgacaaaa ccaaatgaaa
    10501 cagcaataaa aatccggcga ataacggcag taaagaggtt ttgaatcgtt tttcaaacat
    10561 tcttgacact ccttatttga ttttttgaag acttacttcg gagtcaaaaa tccctcttac
    10621 ttcattcttc cgcttcctcc tttcaaaccg atgtgaagac tggagaattt tgttaattct
    10681 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
    10741 gtttcttaga cgtcaggtgg cacttttcgg ggaaatg
//
