LOCUS       V005340                10749 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005340
VERSION     V005340
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10749)
  AUTHORS   Goodson JR, Klupt SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 10749)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10749)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..108
                     /label="AmpR promoter"
     CDS             109..966
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1140..1728
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1978..2757)
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     misc_feature    3866..4866
                     /pseudo=""
                     /note="amyE; amyE back; amyE homology for integration into
                     B. amyloliquefaciens"
     CDS             complement(5240..5887)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      6197..6338
                     /note="PdfnA; promoter region of dfnA from B.
                     amyloliquefaciens FZB42"
                     /regulatory_class="promoter"
     5'UTR           6346..6746
                     /note="dfnA leader region; dfnA leader region from B.
                     amyloliquefaciens FZB42"
     CDS             6762..7478
                     /label="mVenus"
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     regulatory      7488..7521
                     /label="YFP_terminator"
                     /note="YFP_terminator"
                     /regulatory_class="terminator"
     CDS             complement(7595..8125)
                     /codon_start=1
                     /gene="loaP"
                     /product="LoaP"
                     /label="loaP"
                     /note="LoaP; LoaP NusG-like antiterminator protein"
                     /protein_id="APU90162.1"
                     /translation="MKWYALFVESGKEETVQKFLRLQFDEQALYSIIPKKKVTERKAGI
                     KYEALKKMFPGYVLFKTKMTERTFHKIKELPISCRIVNNGAYYSKERKTYFTTIKDEEI
                     LPIIRLIGEGDTVDYSKVYIENSKVTVASGPLKGMEGIIKKIDKRKRRAKICLSFMGLD
                     KMVNVGIEVLSKP"
     gene            complement(7595..8125)
                     /gene="loaP"
                     /label="loaP"
     CDS             complement(8154..8213)
                     /codon_start=1
                     /gene="mini-xylA"
                     /product="hypothetical protein"
                     /label="mini-xylA"
                     /note="mini xylA ORF"
                     /protein_id="APU90163.1"
                     /translation="MAQSHSSSVNYFVSVNKVV"
     gene            complement(8154..8213)
                     /gene="mini-xylA"
                     /label="mini-xylA"
                     /note="xylA"
     protein_bind    complement(8283..8293)
                     /label="XylR binding site"
                     /bound_moiety="XylR"
                     /note="xylO operator"
     protein_bind    complement(8297..8307)
                     /label="XylR binding site"
                     /bound_moiety="XylR"
                     /note="xylO operator"
     regulatory      complement(8317..8322)
                     /label="-10 (sigA)"
                     /note="-10 (sigA)"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(8340..8347)
                     /label="-35 (sigA)"
                     /note="-35 (sigA)"
                     /regulatory_class="minus_35_signal"
     CDS             8574..9626
                     /codon_start=1
                     /gene="xylR"
                     /product="XylR Xylose Repressor protein"
                     /label="xylR"
                     /note="XylR"
                     /protein_id="APU90164.1"
                     /translation="MTGLNKSTVSSQVNTLMKENLVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSIGIDVGVDYISGILTDLEGTIILDQHHHLESNSPEITKDILIDMIHHFITRMPQSP
                     YGLIGIGICVPGLIDKNQKIVFTPNSNWRDIDLKSFIQEKFNVPVFIENEANAGAYGEK
                     VFGAAKNHNNIIYASISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKVSYQDIIDLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAIILRNSIIESHPMVLNSIRSEVSSRVYPQLGNSYELLPSSLGKNAPALGMSSIV
                     IEHFLDIVKM"
     gene            8574..9626
                     /gene="xylR"
                     /label="xylR"
     misc_feature    9634..10533
                     /pseudo=""
                     /note="amyE; amyE front; amyE homology for integration into
                     B. amyloliquefaciens"
ORIGIN
        1 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
       61 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      121 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      181 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      241 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      301 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
      361 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      421 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      481 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      541 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
      601 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
      661 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
      721 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      781 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
      841 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      901 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      961 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1021 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1081 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1141 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1201 gcggtggttt gtttgccgga tcaagagcta tcaactcttt ttccgaaggt aactggcttc
     1261 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1321 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1381 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1441 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1501 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1561 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1621 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1681 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1741 gcggcccgac ctcgagctgg atacttcccg tccgccaggg ggacatgccg gcgatgctga
     1801 aggtcgcgcg cattcccgat gaagaggccg gttaccgcct gtttgaggat atagtaatct
     1861 ttctaaatag ctttggattg gaggagtatg gccactaata ctaagttcag ctaataaaaa
     1921 aatttgctaa agaactccag aaaagtaagc acctgttatt gcaataaaat tagcctaatt
     1981 gagagaagtt tctatagaat ttttcatata cttaacgagt gctttcacct ttgaatatag
     2041 tccttcccac ttatcatcac actctccccg atagcctttt ctagctatat ccagtaaagt
     2101 tacatgctct ttaggtaaaa gaggtatagc ccattctgca gcgacatctt tcgaggtaat
     2161 ttcaccagta gtcactgttt gccacattcg agctagggtt aaaattacat tacgctcatc
     2221 accttttatc ccctcaatta gttctggcaa agaatcctta attgctcttc gaatatctgt
     2281 caaaggtacg gagacaagta tacttgaaga atcaggacca aatagagaaa tactattctt
     2341 tcttgcttgt gctaaaacaa tagccaaatc aggatcatag cttggttcct gaatttgtcc
     2401 attctcaaat tcacccctga gccactcacc gtatataaat tctctttttg gaggatattg
     2461 ccaagggaca acttcactcc tatttataac cgtaacttca agtggtctaa cagaatccgt
     2521 atttccaatc tttcctgata tagtcattag tctttctgtt agtttttttc gagttaattg
     2581 aggtaaacta tgattcacga cgactagaac atctacatcg ctgttaatgc gtaaaccacc
     2641 atttactgct gaaccaaata gatatactcc aactattgaa cttccaaata aatcttttac
     2701 gatttttaat gtttgaatcg cttgatttgg tatttttccg ttaatcaaat tgctcatgat
     2761 ttcacctcgt tgattatgtt catataaagt ttatattgat actcaattta cttaccctag
     2821 attggacata tacttaaatt actgttcaat aaagctgacc gttagcgttt aagtacatcc
     2881 tttcacaatt tgtctacaga ttaataatta ttctttatta tacagatcga tcctctagac
     2941 ctaggcctta agatctgatc atatgcatcc gcgggcccgg gttaacgcgt aatccatgga
     3001 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     3061 tccggtgccc tgaatgaact gcagaaagag ctggtagttg gcgcactgtt cgaagaactg
     3121 ccgatgtcca gtaagattct tactatgctg gttgaaccgg atgctggtaa agctacttgg
     3181 gttgctgctt ctacttatgg taccgataca actactggtg aggaagttaa aggagctctt
     3241 aaagaaatcc acttcagtag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc
     3301 agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg
     3361 cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg
     3421 cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
     3481 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
     3541 agcctcgcgt cgcgactaag aaaatgccgt caaatccgct cgccatgact tcactaacga
     3601 tgcctttgaa aatcttcaag ttcttttcta ctaattcaag gcgtgtctca ccaggttttt
     3661 ggtttgctcc ggcgcaaatg cagacaatat cagcatcctt gcagggtatg tttctctttg
     3721 atgtcttttt gtttgtgaag tatttcacat ttatattgtg caacacttca caaacttttg
     3781 caagagaaaa gttttgtctg atttatgaac aaaaaagaaa ccatcattga tggtttcttt
     3841 cggtaagtcc cgtctagcct tgcccttaat gcggaagata accattcaaa ccggagttgt
     3901 tatacaaacc attctgcaca taatcaaagc cgggctggtt ctgtccgggc acttgggcgc
     3961 tgccattgtt aaaaatcact ttggcattgg ccgtatccgt attctcaggc agcgtcagcg
     4021 tgtacattcc atttgcattc ttagtcattg ctttccccgg ccacgatccg gttaattcta
     4081 tggccctgcc tccatcatgt ttgtaaatat aagcatttac ctggccccaa tggtccggat
     4141 tttgatagcc aatgatgttg gtttgggacg ggtctttttt gacaaatgtg tattcttggg
     4201 ttctcgctgc accctcgccg ttcgttccgg ttaatttgat gttgtatgtc gtgccgattg
     4261 gatctccttt cccgatcgtt agtcggtctc catccttaaa tgctgtctgc tgcccattat
     4321 tgatttggta aacggctttt gttgttttcg catttgcacg cagggtgacc gtcagctgat
     4381 cattgaaaga atggactgcc cccgtttggt aattctcaag aaagacatga ggcgcatttc
     4441 caatatcatc aggataaaga acagccgcgg atctggcatt gatcgtacct gtcagtttgc
     4501 cgttcgctac ttgaaatgaa ccggcgccgg ccctattatc atacctgccg tcaggtaatt
     4561 tcgctgaagt attgatggtg acagaagacg atcccgcatt tgccagcaca acgccttttg
     4621 agccgcgctg attcataaag acttggttgt tcccattcgg attcgagagt tcctcgggct
     4681 gcccggccat tacattgtga aatgtattga ccgcagtgat cgcctgatct ttaaataagg
     4741 cgctcccgcg atctcctatt tgactttttc cgggaaatct tacaccattt cctccgccct
     4801 caggtctgga aaagaaaaga ggcgtgcttc ctgagcggga accaatcact gcccagccta
     4861 aacgaacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4921 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4981 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     5041 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     5101 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggagctg taatataaaa
     5161 accttcttca actaacgggg caggttagtg acattagaaa accgactgta aaaagtacag
     5221 tcggcattat ctcatattat aaaagccagt cattaggcct atctgacaat tcctgaatag
     5281 agttcataaa caatcctgca tgataaccat cacaaacaga atgatgtacc tgtaaagata
     5341 gcggtaaata tattgaatta cctttattaa tgaattttcc tgctgtaata atgggtagaa
     5401 ggtaattact attattattg atatttaagt taaacccagt aaatgaagtc catggaataa
     5461 tagaaagaga aaaagcattt tcaggtatag gtgttttggg aaacaatttc cccgaaccat
     5521 tatatttctc tacatcagaa aggtataaat cataaaactc tttgaagtca ttctttacag
     5581 gagtccaaat accagagaat gttttagata caccatcaaa aattgtataa agtggctcta
     5641 acttatccca ataacctaac tctccgtcgc tattgtaacc agttctaaaa gctgtatttg
     5701 agtttatcac ccttgtcact aagaaaataa atgcagggta aaatttatat ccttcttgtt
     5761 ttatgtttcg gtataaaaca ctaatatcaa tttctgtggt tatactaaaa gtcgtttgtt
     5821 ggttcaaata atgattaaat atctcttttc tcttccaatt gtctaaatca attttattaa
     5881 agttcatttg atatgcctcc taaattttta tctaaagtga atttaggagg cttacttgtc
     5941 tgctttcttc attagaatca atcctttttt aaaagtcaat attactgtaa cataaatata
     6001 tattttaaaa atatcccact ttatccaatt ttcgtttgtt gaactaatgg gtgctttagt
     6061 tgaagaataa agaccacatt aaaaaatgtg gtcttttgtg tttttttaaa ggatttgagc
     6121 gtagcgaaaa atccttttct ttcttatctt gataataagg gtaactattg ccgatgataa
     6181 gctgtcaaac atgagggaca tgaatagggt tcagggaatt atctgacatt tatcccgaaa
     6241 caattatgcc gactgtccac cgaacataaa actgtggaat aaatgtcaga aaataacgaa
     6301 ataaaacctt gacattatta tctataaatt gatattattc tctttaaata gcaactattt
     6361 gttaaattta ttaaattgat gtaaagcacc tattaaattt caatcgctgt ttcggcacgt
     6421 tgaagaggaa atagggatta taaaaatgat tgtagtgtat atatctacat atacatgata
     6481 agggagaagt gtgccgtcat ggctgttttc gggcatattg gcactgcaac ttgtcatgcg
     6541 aaacattcag taaacaagca agatgtcttt atataaatta tgttagaaaa ggaaaacaca
     6601 atgcgtttgc gatttttaaa cctgtgctga atcgcaaacg cgtttgtgtt tttattttta
     6661 tgccggtgtt tacaccagtc aatatatgaa ataagagacg tttttgtatg gagatccaca
     6721 tgtgatatac aaaattggcg atgcggaagg aggaaagtca cattatgagc aaaggtgaag
     6781 aactgttcac cggcgttgtg ccaattctgg ttgagctgga tggtgacgtg aatggccaca
     6841 aattttccgt gtctggtgaa ggcgagtgtg atgctactta tggcaaactg actctgaaac
     6901 tgatctgtac caccggcaaa ctgcctgttc cgtggccaac tctggtcact actctgggtt
     6961 acggcctgat gtgttttgcg cgttacccgg atcacatgaa acagcatgac ttcttcaaat
     7021 ctgccatgcc ggaaggctat gtccaagaac gtacgatctt tttcaaggac gacggcaact
     7081 ataaaacccg tgccgaagtt aaattcgagg gtgacaccct ggttaaccgc atcgaactga
     7141 aaggcattga cttcaaagag gacggcaaca ttctgggtca caagctggaa tacaactaca
     7201 actcccacaa cgtttacatt actgctgaca agcagaaaaa cggcatcaaa gcaaacttca
     7261 agatccgtca caacattgaa gatggtggcg tacagctggc agatcactac cagcagaaca
     7321 ctccaatcgg tgatggccca gtactgctgc cagataacca ttacctgtcc taccagagca
     7381 aactgtctaa agacccgaac gaaaaacgtg accacatggt actgctggaa tttgttaccg
     7441 cggcaggcat tacccacggt atggacgaac tgtataaata agctagcaaa aaccccgccc
     7501 ctgacagggc ggggtttttt ttgaattccg atcagaccag tttttaacaa aaaaagcatc
     7561 aacggcacat gctgttgatg ctttttttga tatgctaggg tttggagagc acttcaattc
     7621 cgacgttgac cattttgtcc agtcccataa aagacaggca gattttggcg cgtcttttcc
     7681 ttttatcgat ctttttaata atgccttcca ttcctttaag aggccctgag gcaacggtta
     7741 cttttgaatt ttcaatatag acttttgaat aatctacagt gtctccttct ccgatcagcc
     7801 tgataatagg gaggatttct tcatccttaa ttgtcgtgaa ataggtttta cgctctttgg
     7861 aataatatgc gccgttattc acgattctgc atgaaatggg gagttctttg attttatgaa
     7921 atgtccgctc tgtcatcttc gttttaaaca gcacatatcc cggaaacatc tttttgagtg
     7981 cctcgtattt gattccggct tttcgttctg tgactttttt cttgggtatg attgagtaga
     8041 gagcctgctc atcaaactgc agacgcagaa atttttgaac tgtttcttct tttccggact
     8101 ctacaaaaag tgcgtaccat ttcatgactg ggtcctcctt ctgtcgactt aatttaaacc
     8161 actttgttaa cgcttacaaa atagttaact gaactagaat gagattgagc catgttattt
     8221 cctccttaaa aataaattca aatacagatg cattttatgt catattgtaa gtaagttgca
     8281 cattagtttg ttgcccaaac aaactaactt attttaatct taaataacct catcagtatt
     8341 ttcaatattt tttttagttt ttcatgaaca cattagatat aataaaggta agattcgcta
     8401 tgtactaaat ctcatactga acacaaaaaa ttttaactta atttaaaggt tatacaaatg
     8461 gagtggatga agtggatatc gcagatcaaa cctttgtcaa aaaagtaaat caaaagttat
     8521 tattaaaaga aatacttaag aattcaccta tttcaagagc aaaattatct gaaatgactg
     8581 gattaaataa atcaactgtt tcatcacagg taaacacgct gatgaaagaa aatcttgtat
     8641 ttgaaatagg tcaaggacaa tcaagtggcg gaagaagacc tgtcatgctt gtttttaata
     8701 aaaaggcagg atactccata ggaatagatg ttggtgtgga ttatattagt ggcattttaa
     8761 cagaccttga aggaacgatc attcttgatc aacatcacca tttagaatcg aattctccag
     8821 aaataactaa agacatttta attgatatga ttcatcactt tattacgcgt atgccacaat
     8881 ctccgtacgg gcttatcggt ataggcatat gcgtgcctgg actaattgat aaaaatcaaa
     8941 aaattgtttt cactccgaac tccaactgga gagatattga cttaaaatct ttcatacaag
     9001 agaagttcaa tgtgcctgtt tttattgaaa atgaggcaaa tgctggcgcg tatggagaaa
     9061 aagtatttgg tgctgcaaaa aatcacaata acattattta tgctagtatc agtacaggta
     9121 tagggatcgg tgttattatc aacaatcatt tatatagagg ggtaagcgga ttctctggag
     9181 aaatgggaca tatgacaata gactttaatg gtcctaaatg cagttgcgga aaccgaggtt
     9241 gctgggaatt gtatgcttca gagaaggctt tattaaaatc tcttcagact aaagagaaaa
     9301 aagtgtccta tcaagatatc atagacctcg cccatctgaa tgatatcggc accttaaatg
     9361 cattacagaa tttcggattc tatttaggaa ttggccttac taatattcta aatacattca
     9421 atccacaagc catcatttta agaaacagca taattgaatc acatcctatg gttttaaatt
     9481 caattagaag tgaagtgtcg tccagggttt atccccaatt aggcaatagc tatgaattat
     9541 taccatcttc cttaggaaag aatgcaccgg cattaggaat gtcttccatt gttattgaac
     9601 attttctaga tatcgttaaa atgtaaaagc ttcgtccgca gacacgtcag atgcataatg
     9661 ggagatattc gacacactca gattacggtt ctttaaagcg gatctgatgg aatgcccgta
     9721 gttagaagcc gtcacattca tataattcgc ataagcagta tctctggacg cgctgtcttg
     9781 caggatttct ccgtattgga actccgctga tgtatttgtg atattcggcc aaaattggct
     9841 gccgtaattc ccgtcatccg gaagctctat atgcttggcg gcatcatagc ggaacccgtc
     9901 tgctccgtca ttcaatgctc tttccaagaa acgtttcaga tagacctgca cctcagtatt
     9961 ctgtgtattc caatcataca gcccgagcaa tgaattttga gtgacgtccc atcgatccga
    10021 ccaattttta atttgtgtgt ttccatgggt ccagtttgga atacgcttaa tctcgtcaga
    10081 aatcgcgcca taatcgctgg tagtatgatt gataaccgca tcgacaatga cttttacgcc
    10141 atatttttcc gccgctgcac acatgtcctt aaattcttgt tcagtgccta agtaacggtt
    10201 gccgatttgg tacgatgtcg gctgatacag ccagtaccag ttcctcatgc ttttatctcc
    10261 ttggttccct tcctttactt ggttaatcgg agacgtctga atggctgcat agcccgcatc
    10321 acgaatatct ttcatatttt gtgttaacgt attgaacgac caattccatg catgaaggat
    10381 ggtcccgttt ttgaccgatg acgcggtcac cttattcgat ttgtttgcag tttcagcgtt
    10441 tgcagccgcc gggcctgaca aaaccaaatg aaacagcaat aaaaatccgg cgaataacgg
    10501 cagtaaagag gttttgaatc gtttttcaaa cattcttgac actccttatt tgattttttg
    10561 aagacttact tcggagtcaa aaatccctct tacttcattc ttccgcttcc tcctttcaaa
    10621 ccgatgtgaa gactggagaa ttttgttaat tcttgaagac gaaagggcct cgtgatacgc
    10681 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
    10741 cggggaaat
//
