LOCUS       V005341                10900 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005341
VERSION     V005341
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Goodson JR, Klupt SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 10900)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10900)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..107
                     /label="AmpR promoter"
     CDS             108..965
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1139..1727
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1977..2756)
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     misc_feature    3865..4865
                     /pseudo=""
                     /note="amyE; amyE back; amyE homology for integration into
                     B. amyloliquefaciens"
     CDS             complement(5239..5886)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      6196..6337
                     /label="PdfnA"
                     /note="PdfnA"
                     /regulatory_class="promoter"
     5'UTR           6345..6745
                     /note="dfnA leader region; dfnA leader region"
     terminator      6745..6792
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     regulatory      6794..6839
                     /label="lambda tR2 terminator"
                     /note="lambda tR2 terminator"
                     /regulatory_class="terminator"
     terminator      complement(6843..6886)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      6897..6914
                     /label="Winkler Std. RBS"
                     /note="Winkler Std. RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             6912..7628
                     /label="mVenus"
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     regulatory      7638..7671
                     /label="YFP_terminator"
                     /note="YFP_terminator"
                     /regulatory_class="terminator"
     misc_feature    7679..7697
                     /label="amyE-front"
                     /note="amyE-front"
     CDS             complement(7745..8275)
                     /codon_start=1
                     /gene="loaP"
                     /product="LoaP"
                     /label="loaP"
                     /note="LoaP; LoaP NusG-like antiterminator protein"
                     /protein_id="APU90169.1"
                     /translation="MKWYALFVESGKEETVQKFLRLQFDEQALYSIIPKKKVTERKAGI
                     KYEALKKMFPGYVLFKTKMTERTFHKIKELPISCRIVNNGAYYSKERKTYFTTIKDEEI
                     LPIIRLIGEGDTVDYSKVYIENSKVTVASGPLKGMEGIIKKIDKRKRRAKICLSFMGLD
                     KMVNVGIEVLSKP"
     gene            complement(7745..8275)
                     /gene="loaP"
                     /label="loaP"
     CDS             complement(8304..8363)
                     /codon_start=1
                     /gene="mini-xylA"
                     /product="hypothetical protein"
                     /label="mini-xylA"
                     /note="mini xylA ORF"
                     /protein_id="APU90170.1"
                     /translation="MAQSHSSSVNYFVSVNKVV"
     gene            complement(8304..8363)
                     /gene="mini-xylA"
                     /label="mini-xylA"
                     /note="xylA"
     protein_bind    complement(8433..8443)
                     /label="XylR binding site"
                     /bound_moiety="XylR"
                     /note="xylO operator"
     protein_bind    complement(8447..8457)
                     /label="XylR binding site"
                     /bound_moiety="XylR"
                     /note="xylO operator"
     regulatory      complement(8467..8472)
                     /label="-10 (sigA)"
                     /note="-10 (sigA)"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(8490..8497)
                     /label="-35 (sigA)"
                     /note="-35 (sigA)"
                     /regulatory_class="minus_35_signal"
     CDS             8724..9776
                     /codon_start=1
                     /gene="xylR"
                     /product="XylR Xylose Repressor protein"
                     /label="xylR"
                     /note="XylR"
                     /protein_id="APU90171.1"
                     /translation="MTGLNKSTVSSQVNTLMKENLVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSIGIDVGVDYISGILTDLEGTIILDQHHHLESNSPEITKDILIDMIHHFITRMPQSP
                     YGLIGIGICVPGLIDKNQKIVFTPNSNWRDIDLKSFIQEKFNVPVFIENEANAGAYGEK
                     VFGAAKNHNNIIYASISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKVSYQDIIDLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAIILRNSIIESHPMVLNSIRSEVSSRVYPQLGNSYELLPSSLGKNAPALGMSSIV
                     IEHFLDIVKM"
     gene            8724..9776
                     /gene="xylR"
                     /label="xylR"
     misc_feature    9784..10683
                     /pseudo=""
                     /note="amyE; amyE front; amyE homology for integration into
                     B. amyloliquefaciens"
ORIGIN
        1 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
       61 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      121 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      181 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      241 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      301 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
      361 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      421 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      481 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      541 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      601 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
      661 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
      721 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
      781 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
      841 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
      901 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
      961 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1021 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1081 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1141 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1201 cggtggtttg tttgccggat caagagctat caactctttt tccgaaggta actggcttca
     1261 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1321 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1381 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1441 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1501 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1561 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1621 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1681 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1741 cggcccgacc tcgagctgga tacttcccgt ccgccagggg gacatgccgg cgatgctgaa
     1801 ggtcgcgcgc attcccgatg aagaggccgg ttaccgcctg tttgaggata tagtaatctt
     1861 tctaaatagc tttggattgg aggagtatgg ccactaatac taagttcagc taataaaaaa
     1921 atttgctaaa gaactccaga aaagtaagca cctgttattg caataaaatt agcctaattg
     1981 agagaagttt ctatagaatt tttcatatac ttaacgagtg ctttcacctt tgaatatagt
     2041 ccttcccact tatcatcaca ctctccccga tagccttttc tagctatatc cagtaaagtt
     2101 acatgctctt taggtaaaag aggtatagcc cattctgcag cgacatcttt cgaggtaatt
     2161 tcaccagtag tcactgtttg ccacattcga gctagggtta aaattacatt acgctcatca
     2221 ccttttatcc cctcaattag ttctggcaaa gaatccttaa ttgctcttcg aatatctgtc
     2281 aaaggtacgg agacaagtat acttgaagaa tcaggaccaa atagagaaat actattcttt
     2341 cttgcttgtg ctaaaacaat agccaaatca ggatcatagc ttggttcctg aatttgtcca
     2401 ttctcaaatt cacccctgag ccactcaccg tatataaatt ctctttttgg aggatattgc
     2461 caagggacaa cttcactcct atttataacc gtaacttcaa gtggtctaac agaatccgta
     2521 tttccaatct ttcctgatat agtcattagt ctttctgtta gtttttttcg agttaattga
     2581 ggtaaactat gattcacgac gactagaaca tctacatcgc tgttaatgcg taaaccacca
     2641 tttactgctg aaccaaatag atatactcca actattgaac ttccaaataa atcttttacg
     2701 atttttaatg tttgaatcgc ttgatttggt atttttccgt taatcaaatt gctcatgatt
     2761 tcacctcgtt gattatgttc atataaagtt tatattgata ctcaatttac ttaccctaga
     2821 ttggacatat acttaaatta ctgttcaata aagctgaccg ttagcgttta agtacatcct
     2881 ttcacaattt gtctacagat taataattat tctttattat acagatcgat cctctagacc
     2941 taggccttaa gatctgatca tatgcatccg cgggcccggg ttaacgcgta atccatggat
     3001 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     3061 ccggtgccct gaatgaactg cagaaagagc tggtagttgg cgcactgttc gaagaactgc
     3121 cgatgtccag taagattctt actatgctgg ttgaaccgga tgctggtaaa gctacttggg
     3181 ttgctgcttc tacttatggt accgatacaa ctactggtga ggaagttaaa ggagctctta
     3241 aagaaatcca cttcagtagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca
     3301 gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc
     3361 ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc
     3421 gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
     3481 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
     3541 gcctcgcgtc gcgactaaga aaatgccgtc aaatccgctc gccatgactt cactaacgat
     3601 gcctttgaaa atcttcaagt tcttttctac taattcaagg cgtgtctcac caggtttttg
     3661 gtttgctccg gcgcaaatgc agacaatatc agcatccttg cagggtatgt ttctctttga
     3721 tgtctttttg tttgtgaagt atttcacatt tatattgtgc aacacttcac aaacttttgc
     3781 aagagaaaag ttttgtctga tttatgaaca aaaaagaaac catcattgat ggtttctttc
     3841 ggtaagtccc gtctagcctt gcccttaatg cggaagataa ccattcaaac cggagttgtt
     3901 atacaaacca ttctgcacat aatcaaagcc gggctggttc tgtccgggca cttgggcgct
     3961 gccattgtta aaaatcactt tggcattggc cgtatccgta ttctcaggca gcgtcagcgt
     4021 gtacattcca tttgcattct tagtcattgc tttccccggc cacgatccgg ttaattctat
     4081 ggccctgcct ccatcatgtt tgtaaatata agcatttacc tggccccaat ggtccggatt
     4141 ttgatagcca atgatgttgg tttgggacgg gtcttttttg acaaatgtgt attcttgggt
     4201 tctcgctgca ccctcgccgt tcgttccggt taatttgatg ttgtatgtcg tgccgattgg
     4261 atctcctttc ccgatcgtta gtcggtctcc atccttaaat gctgtctgct gcccattatt
     4321 gatttggtaa acggcttttg ttgttttcgc atttgcacgc agggtgaccg tcagctgatc
     4381 attgaaagaa tggactgccc ccgtttggta attctcaaga aagacatgag gcgcatttcc
     4441 aatatcatca ggataaagaa cagccgcgga tctggcattg atcgtacctg tcagtttgcc
     4501 gttcgctact tgaaatgaac cggcgccggc cctattatca tacctgccgt caggtaattt
     4561 cgctgaagta ttgatggtga cagaagacga tcccgcattt gccagcacaa cgccttttga
     4621 gccgcgctga ttcataaaga cttggttgtt cccattcgga ttcgagagtt cctcgggctg
     4681 cccggccatt acattgtgaa atgtattgac cgcagtgatc gcctgatctt taaataaggc
     4741 gctcccgcga tctcctattt gactttttcc gggaaatctt acaccatttc ctccgccctc
     4801 aggtctggaa aagaaaagag gcgtgcttcc tgagcgggaa ccaatcactg cccagcctaa
     4861 acgaacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     4921 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     4981 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     5041 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     5101 cgccggtgat gccggccacg atgcgtccgg cgtagaggat ctggagctgt aatataaaaa
     5161 ccttcttcaa ctaacggggc aggttagtga cattagaaaa ccgactgtaa aaagtacagt
     5221 cggcattatc tcatattata aaagccagtc attaggccta tctgacaatt cctgaataga
     5281 gttcataaac aatcctgcat gataaccatc acaaacagaa tgatgtacct gtaaagatag
     5341 cggtaaatat attgaattac ctttattaat gaattttcct gctgtaataa tgggtagaag
     5401 gtaattacta ttattattga tatttaagtt aaacccagta aatgaagtcc atggaataat
     5461 agaaagagaa aaagcatttt caggtatagg tgttttggga aacaatttcc ccgaaccatt
     5521 atatttctct acatcagaaa ggtataaatc ataaaactct ttgaagtcat tctttacagg
     5581 agtccaaata ccagagaatg ttttagatac accatcaaaa attgtataaa gtggctctaa
     5641 cttatcccaa taacctaact ctccgtcgct attgtaacca gttctaaaag ctgtatttga
     5701 gtttatcacc cttgtcacta agaaaataaa tgcagggtaa aatttatatc cttcttgttt
     5761 tatgtttcgg tataaaacac taatatcaat ttctgtggtt atactaaaag tcgtttgttg
     5821 gttcaaataa tgattaaata tctcttttct cttccaattg tctaaatcaa ttttattaaa
     5881 gttcatttga tatgcctcct aaatttttat ctaaagtgaa tttaggaggc ttacttgtct
     5941 gctttcttca ttagaatcaa tcctttttta aaagtcaata ttactgtaac ataaatatat
     6001 attttaaaaa tatcccactt tatccaattt tcgtttgttg aactaatggg tgctttagtt
     6061 gaagaataaa gaccacatta aaaaatgtgg tcttttgtgt ttttttaaag gatttgagcg
     6121 tagcgaaaaa tccttttctt tcttatcttg ataataaggg taactattgc cgatgataag
     6181 ctgtcaaaca tgagggacat gaatagggtt cagggaatta tctgacattt atcccgaaac
     6241 aattatgccg actgtccacc gaacataaaa ctgtggaata aatgtcagaa aataacgaaa
     6301 taaaaccttg acattattat ctataaattg atattattct ctttaaatag caactatttg
     6361 ttaaatttat taaattgatg taaagcacct attaaatttc aatcgctgtt tcggcacgtt
     6421 gaagaggaaa tagggattat aaaaatgatt gtagtgtata tatctacata tacatgataa
     6481 gggagaagtg tgccgtcatg gctgttttcg ggcatattgg cactgcaact tgtcatgcga
     6541 aacattcagt aaacaagcaa gatgtcttta tataaattat gttagaaaag gaaaacacaa
     6601 tgcgtttgcg atttttaaac ctgtgctgaa tcgcaaacgc gtttgtgttt ttatttttat
     6661 gccggtgttt acaccagtca atatatgaaa taagagacgt ttttgtatgg agatccacat
     6721 gtgatataca aaattggcga tgcggtagca taaccccttg gggcctctaa acgggtcttg
     6781 aggggttttt tgtactgagc taataacagg cctgctggta atcgcaggcc tttttatttc
     6841 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttaatctg gctagtaagg
     6901 aggaaagtca cattatgagc aaaggtgaag aactgttcac cggcgttgtg ccaattctgg
     6961 ttgagctgga tggtgacgtg aatggccaca aattttccgt gtctggtgaa ggcgagggtg
     7021 atgctactta tggcaaactg actctgaaac tgatctgtac caccggcaaa ctgcctgttc
     7081 cgtggccaac tctggtcact actctgggtt acggcctgat gtgttttgcg cgttacccgg
     7141 atcacatgaa acagcatgac ttcttcaaat ctgccatgcc ggaaggctat gtccaagaac
     7201 gtacgatctt tttcaaggac gacggcaact ataaaacccg tgccgaagtt aaattcgagg
     7261 gtgacaccct ggttaaccgc atcgaactga aaggcattga cttcaaagag gacggcaaca
     7321 ttctgggtca caagctggaa tacaactaca actcccacaa cgtttacatt actgctgaca
     7381 agcagaaaaa cggcatcaaa gcaaacttca agatccgtca caacattgaa gatggtggcg
     7441 tacagctggc agatcactac cagcagaaca ctccaatcgg tgatggccca gtactgctgc
     7501 cagataacca ttacctgtcc taccagagca aactgtctaa agacccgaac gaaaaacgtg
     7561 accacatggt actgctggaa tttgttaccg cggcaggcat tacccacggt atggacgaac
     7621 tgtataaata agctagcaaa aaccccgccc ctgacagggc ggggtttttt ttgaattccg
     7681 atcagaccag tttttaacaa aaaaagcatc aacggcacat gctgttgatg ctttttttga
     7741 tatgctaggg tttggagagc acttcaattc cgacgttgac cattttgtcc agtcccataa
     7801 aagacaggca gattttggcg cgtcttttcc ttttatcgat ctttttaata atgccttcca
     7861 ttcctttaag aggccctgag gcaacggtta cttttgaatt ttcaatatag acttttgaat
     7921 aatctacagt gtctccttct ccgatcagcc tgataatagg gaggatttct tcatccttaa
     7981 ttgtcgtgaa ataggtttta cgctctttgg aataatatgc gccgttattc acgattctgc
     8041 atgaaatggg gagttctttg attttatgaa atgtccgctc tgtcatcttc gttttaaaca
     8101 gcacatatcc cggaaacatc tttttgagtg cctcgtattt gattccggct tttcgttctg
     8161 tgactttttt cttgggtatg attgagtaga gagcctgctc atcaaactgc agacgcagaa
     8221 atttttgaac tgtttcttct tttccggact ctacaaaaag tgcgtaccat ttcatgactg
     8281 ggtcctcctt ctgtcgactt aatttaaacc actttgttaa cgcttacaaa atagttaact
     8341 gaactagaat gagattgagc catgttattt cctccttaaa aataaattca aatacagatg
     8401 cattttatgt catattgtaa gtaagttgca cattagtttg ttgcccaaac aaactaactt
     8461 attttaatct taaataacct catcagtatt ttcaatattt tttttagttt ttcatgaaca
     8521 cattagatat aataaaggta agattcgcta tgtactaaat ctcatactga acacaaaaaa
     8581 ttttaactta atttaaaggt tatacaaatg gagtggatga agtggatatc gcagatcaaa
     8641 cctttgtcaa aaaagtaaat caaaagttat tattaaaaga aatacttaag aattcaccta
     8701 tttcaagagc aaaattatct gaaatgactg gattaaataa atcaactgtt tcatcacagg
     8761 taaacacgct gatgaaagaa aatcttgtat ttgaaatagg tcaaggacaa tcaagtggcg
     8821 gaagaagacc tgtcatgctt gtttttaata aaaaggcagg atactccata ggaatagatg
     8881 ttggtgtgga ttatattagt ggcattttaa cagaccttga aggaacgatc attcttgatc
     8941 aacatcacca tttagaatcg aattctccag aaataactaa agacatttta attgatatga
     9001 ttcatcactt tattacgcgt atgccacaat ctccgtacgg gcttatcggt ataggcatat
     9061 gcgtgcctgg actaattgat aaaaatcaaa aaattgtttt cactccgaac tccaactgga
     9121 gagatattga cttaaaatct ttcatacaag agaagttcaa tgtgcctgtt tttattgaaa
     9181 atgaggcaaa tgctggcgcg tatggagaaa aagtatttgg tgctgcaaaa aatcacaata
     9241 acattattta tgctagtatc agtacaggta tagggatcgg tgttattatc aacaatcatt
     9301 tatatagagg ggtaagcgga ttctctggag aaatgggaca tatgacaata gactttaatg
     9361 gtcctaaatg cagttgcgga aaccgaggtt gctgggaatt gtatgcttca gagaaggctt
     9421 tattaaaatc tcttcagact aaagagaaaa aagtgtccta tcaagatatc atagacctcg
     9481 cccatctgaa tgatatcggc accttaaatg cattacagaa tttcggattc tatttaggaa
     9541 ttggccttac taatattcta aatacattca atccacaagc catcatttta agaaacagca
     9601 taattgaatc acatcctatg gttttaaatt caattagaag tgaagtgtcg tccagggttt
     9661 atccccaatt aggcaatagc tatgaattat taccatcttc cttaggaaag aatgcaccgg
     9721 cattaggaat gtcttccatt gttattgaac attttctaga tatcgttaaa atgtaaaagc
     9781 ttcgtccgca gacacgtcag atgcataatg ggagatattc gacacactca gattacggtt
     9841 ctttaaagcg gatctgatgg aatgcccgta gttagaagcc gtcacattca tataattcgc
     9901 ataagcagta tctctggacg cgctgtcttg caggatttct ccgtattgga actccgctga
     9961 tgtatttgtg atattcggcc aaaattggct gccgtaattc ccgtcatccg gaagctctat
    10021 atgcttggcg gcatcatagc ggaacccgtc tgctccgtca ttcaatgctc tttccaagaa
    10081 acgtttcaga tagacctgca cctcagtatt ctgtgtattc caatcataca gcccgagcaa
    10141 tgaattttga gtgacgtccc atcgatccga ccaattttta atttgtgtgt ttccatgggt
    10201 ccagtttgga atacgcttaa tctcgtcaga aatcgcgcca taatcgctgg tagtatgatt
    10261 gataaccgca tcgacaatga cttttacgcc atatttttcc gccgctgcac acatgtcctt
    10321 aaattcttgt tcagtgccta agtaacggtt gccgatttgg tacgatgtcg gctgatacag
    10381 ccagtaccag ttcctcatgc ttttatctcc ttggttccct tcctttactt ggttaatcgg
    10441 agacgtctga atggctgcat agcccgcatc acgaatatct ttcatatttt gtgttaacgt
    10501 attgaacgac caattccatg catgaaggat ggtcccgttt ttgaccgatg acgcggtcac
    10561 cttattcgat ttgtttgcag tttcagcgtt tgcagccgcc gggcctgaca aaaccaaatg
    10621 aaacagcaat aaaaatccgg cgaataacgg cagtaaagag gttttgaatc gtttttcaaa
    10681 cattcttgac actccttatt tgattttttg aagacttact tcggagtcaa aaatccctct
    10741 tacttcattc ttccgcttcc tcctttcaaa ccgatgtgaa gactggagaa ttttgttaat
    10801 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
    10861 atggtttctt agacgtcagg tggcactttt cggggaaatg
//
