LOCUS       V005344                 7911 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005344
VERSION     V005344
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Goodson JR.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 7911)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7911)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            complement(6..1034)
                     /pseudo=""
                     /gene="amyE"
                     /label="amyE"
                     /note="amyE-back; 3' end of amyE homology"
     CDS             complement(1408..2055)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      2368..2526
                     /gene="yfp"
                     /label="Pconst"
                     /note="Pconst"
                     /regulatory_class="promoter"
     regulatory      2533..2543
                     /gene="yfp"
                     /label="yfp RBS"
                     /note="yfp RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             2548..3264
                     /label="mVenus"
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     regulatory      3274..3307
                     /label="YFP_terminator"
                     /note="YFP_terminator"
                     /regulatory_class="terminator"
     gene            complement(3315..3835)
                     /pseudo=""
                     /gene="amyE"
                     /label="amyE"
                     /note="amyE-front; 5' end of amyE homology"
     promoter        4055..4159
                     /label="AmpR promoter"
     CDS             4160..5017
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5191..5779
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6029..6808)
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
ORIGIN
        1 tgccctcaat ggggaagaga accgcttaag cccgagtcat tatataaacc atttagcacg
       61 taatcaaagc caggctgatt ctgaccgggc acttgggcgc tgccattatt aaaaatcact
      121 tttgcgttgg ttgtatccgt gtccgcaggc agcgtcagcg tgtaaattcc gtctgcattt
      181 ttagtcattg gttttccagg ccaagatccg gtcaattcaa ttactcggct cccatcatgt
      241 ttatagatat aagcatttac ctggctccaa tgattcggat tttgatagcc gatggttttg
      301 gccgacgctg gatctctttt aacaaaactg tatttctcgg tcctcgttac accatcactg
      361 ttcgttcctt ttaacatgat ggtgtatgtt ttgccaaatt ggatctcctt ttccgattgt
      421 gaattgatct ccatccttaa acgcctgtcg tctggtccat tattgatttg ataaacggct
      481 tttgttgtat tcgcatctgc acgcaaggta atcgtcagtt gatcattgaa agaatgtgtt
      541 acacctgttt tgtaattctc aaggaaaaca tgaggcgctt ttgcaatatc atcaggataa
      601 agcacagcta cagacctggc attgatcgtg cctgtcagtt taccatcgtt cacttgaaat
      661 gaacccgctc cagctttatt gtcatacctg ccatcaggca attttgttgc cgtattgata
      721 gagacagagg atgaacctgc atttgccagc acaacgccat gtgagccgcg ctgattcata
      781 aatatctggt tgtttccatt cgggttcgag agttcctcag gctgtccagc catcacattg
      841 tgaaatctat tgaccgcagt gatagcctga tcttcaaata aagcactccc gcgatcgcct
      901 atttggcttt tccccgggaa cctcacacca tttccgcctc cctcaggtct ggaaaagaaa
      961 agaggcgtac tgcctgaacg agaagctatc accgcccagc ctaaacggat atcatcatcg
     1021 ctcatccatg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     1081 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     1141 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     1201 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     1261 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tggagctgta
     1321 atataaaaac cttcttcaac taacggggca ggttagtgac attagaaaac cgactgtaaa
     1381 aagtacagtc ggcattatct catattataa aagccagtca ttaggcctat ctgacaattc
     1441 ctgaatagag ttcataaaca atcctgcatg ataaccatca caaacagaat gatgtacctg
     1501 taaagatagc ggtaaatata ttgaattacc tttattaatg aattttcctg ctgtaataat
     1561 gggtagaagg taattactat tattattgat atttaagtta aacccagtaa atgaagtcca
     1621 tggaataata gaaagagaaa aagcattttc aggtataggt gttttgggaa acaatttccc
     1681 cgaaccatta tatttctcta catcagaaag gtataaatca taaaactctt tgaagtcatt
     1741 ctttacagga gtccaaatac cagagaatgt tttagataca ccatcaaaaa ttgtataaag
     1801 tggctctaac ttatcccaat aacctaactc tccgtcgcta ttgtaaccag ttctaaaagc
     1861 tgtatttgag tttatcaccc ttgtcactaa gaaaataaat gcagggtaaa atttatatcc
     1921 ttcttgtttt atgtttcggt ataaaacact aatatcaatt tctgtggtta tactaaaagt
     1981 cgtttgttgg ttcaaataat gattaaatat ctcttttctc ttccaattgt ctaaatcaat
     2041 tttattaaag ttcatttgat atgcctccta aatttttatc taaagtgaat ttaggaggct
     2101 tacttgtctg ctttcttcat tagaatcaat ccttttttaa aagtcaatat tactgtaaca
     2161 taaatatata ttttaaaaat atcccacttt atccaatttt cgtttgttga actaatgggt
     2221 gctttagttg aagaataaag accacattaa aaaatgtggt cttttgtgtt tttttaaagg
     2281 atttgagcgt agcgaaaaat ccttttcttt cttatcttga taataagggt aactattgcc
     2341 gatgataagc tgtcaaacat gaggatccgt agcccttgcc tacctagctt ccaagaaaga
     2401 tatccttaca gcacaagagc ggaaagatgt tttgttctac atccagaaca acctctgcta
     2461 aaattcctga aaaattttcg aaaaagttgt tgactttatc tacaaggtgt ggcataatgt
     2521 gtgtgcaagc ttaaggagga aagtcacatt atgagcaaag gtgaagaact gttcaccggc
     2581 gttgtgccaa ttctggttga gctggatggt gacgtgaatg gccacaaatt ttccgtgtct
     2641 ggtgaaggcg agggtgatgc tacttatggc aaactgactc tgaaactgat ctgtaccacc
     2701 ggcaaactgc ctgttccgtg gccaactctg gtcactactc tgggttacgg cctgatgtgt
     2761 tttgcgcgtt acccggatca catgaaacag catgacttct tcaaatctgc catgccggaa
     2821 ggctatgtcc aagaacgtac gatctttttc aaggacgacg gcaactataa aacccgtgcc
     2881 gaagttaaat tcgagggtga caccctggtt aaccgcatcg aactgaaagg cattgacttc
     2941 aaagaggacg gcaacattct gggtcacaag ctggaataca actacaactc ccacaacgtt
     3001 tacattactg ctgacaagca gaaaaacggc atcaaagcaa acttcaagat ccgtcacaac
     3061 attgaagatg gtggcgtaca gctggcagat cactaccagc agaacactcc aatcggtgat
     3121 ggcccagtac tgctgccaga taaccattac ctgtcctacc agagcaaact gtctaaagac
     3181 ccgaacgaaa aacgtgacca catggtactg ctggaatttg ttaccgcggc aggcattacc
     3241 cacggtatgg acgaactgta taaataagct agcaaaaacc ccgcccctga cagggcgggg
     3301 ttttttttga attccgatca gaccagtttt taatttgtgt gtttccatgt gtccagtttg
     3361 gaatactctt aacctcattg gaaatcgcgg cataatcact ggtggtatga ttgatgaccg
     3421 cgtcaacaat gacctttatg ccatattctt cagcggctgc acacatttct ttaaattctt
     3481 gttcagtacc taagtaacgg ttgccaattt gatacgatgt cggctgatac agccagtacc
     3541 agttcgacat gcttttatct ccttgattcc cttcctttac ttggttaatc ggagatgtct
     3601 gaatggctgt atatcctgca tcatgaatat ccttcatatt gtgttttaac gtattgaacg
     3661 accaattcca tgcatgaaga atggttccgc ttttgatcga cggtgctgta agctcattcg
     3721 atttgttcgc cgtttcagca ctcgcagccg ccggtcctgc cagaaccaaa tgaaacagca
     3781 ataaaaatcc agcgaataac ggcagtaaag aggttttgaa tcgttttgca aacattcttg
     3841 acactcctta tttgattttt tgaagactta cttcggagtc aaaaatccct cttacttcat
     3901 tcttccgctt cctcctttca aaccgatgtg aagactggag aattttgtta attcttgaag
     3961 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4021 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4081 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4141 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4201 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4261 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4321 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4381 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     4441 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4501 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4561 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4621 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4681 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4741 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4801 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4861 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4921 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4981 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5041 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5101 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5161 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5221 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5281 atcaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5341 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5401 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5461 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5521 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5581 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5641 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5701 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5761 ggggcggagc ctatggaaaa acgccagcaa cgcggcccga cctcgagctg gatacttccc
     5821 gtccgccagg gggacatgcc ggcgatgctg aaggtcgcgc gcattcccga tgaagaggcc
     5881 ggttaccgcc tgtttgagga tatagtaatc tttctaaata gctttggatt ggaggagtat
     5941 ggccactaat actaagttca gctaataaaa aaatttgcta aagaactcca gaaaagtaag
     6001 cacctgttat tgcaataaaa ttagcctaat tgagagaagt ttctatagaa tttttcatat
     6061 acttaacgag tgctttcacc tttgaatata gtccttccca cttatcatca cactctcccc
     6121 gatagccttt tctagctata tccagtaaag ttacatgctc tttaggtaaa agaggtatag
     6181 cccattctgc agcgacatct ttcgaggtaa tttcaccagt agtcactgtt tgccacattc
     6241 gagctagggt taaaattaca ttacgctcat caccttttat cccctcaatt agttctggca
     6301 aagaatcctt aattgctctt cgaatatctg tcaaaggtac ggagacaagt atacttgaag
     6361 aatcaggacc aaatagagaa atactattct ttcttgcttg tgctaaaaca atagccaaat
     6421 caggatcata gcttggttcc tgaatttgtc cattctcaaa ttcacccctg agccactcac
     6481 cgtatataaa ttctcttttt ggaggatatt gccaagggac aacttcactc ctatttataa
     6541 ccgtaacttc aagtggtcta acagaatccg tatttccaat ctttcctgat atagtcatta
     6601 gtctttctgt tagttttttt cgagttaatt gaggtaaact atgattcacg acgactagaa
     6661 catctacatc gctgttaatg cgtaaaccac catttactgc tgaaccaaat agatatactc
     6721 caactattga acttccaaat aaatctttta cgatttttaa tgtttgaatc gcttgatttg
     6781 gtatttttcc gttaatcaaa ttgctcatga tttcacctcg ttgattatgt tcatataaag
     6841 tttatattga tactcaattt acttacccta gattggacat atacttaaat tactgttcaa
     6901 taaagctgac cgttagcgtt taagtacatc ctttcacaat ttgtctacag attaataatt
     6961 attctttatt atacagatcg atcctctaga cctaggcctt aagatctgat catatgcatc
     7021 cgcgggcccg ggttaacgcg taatccatgg atcaagagac aggatgagga tcgtttcgca
     7081 tgattgaaca agatggattg cacgcaggtt ctccggtgcc ctgaatgaac tgcagaaaga
     7141 gctggtagtt ggcgcactgt tcgaagaact gccgatgtcc agtaagattc ttactatgct
     7201 ggttgaaccg gatgctggta aagctacttg ggttgctgct tctacttatg gtaccgatac
     7261 aactactggt gaggaagtta aaggagctct taaagaaatc cacttcagta ggtggcccgg
     7321 ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc
     7381 catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt
     7441 ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg
     7501 tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa
     7561 gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgactaa gaaaatgccg
     7621 tcaaatccgc tcgccatgac ttcactaacg atgcctttga aaatcttcaa gttcttttct
     7681 actaattcaa ggcgtgtctc accaggtttt tggtttgctc cggcgcaaat gcagacaata
     7741 tcagcatcct tgcagggtat gtttctcttt gatgtctttt tgtttgtgaa gtatttcaca
     7801 tttatattgt gcaacacttc acaaactttt gcaagagaaa agttttgtct gatttatgaa
     7861 caaaaaagaa accatcattg atggtttctt tcggtaagtc ccgtctagcc t
//
