LOCUS       40924_26041       10567 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJFR5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Multichromatic Bacterial Photography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Biological Engineering, MIT, 500 Tech 
            Square, Rm 140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 10567)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10567)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2016) Biological Engineering, MIT, 500 Tech Square, Rm 140, 
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..19
                     /label=Promoter K1F (T7 RNAP)
                     /note="Promoter K1F (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             51..1859
                     /label=GUS
                     /note="beta-glucuronidase"
     misc_feature    1902..2061
                     /label=Terminator DT16
                     /note="Terminator DT16"
     regulatory      2112..2130
                     /label=Promoter T3 (T7 RNAP)
                     /note="Promoter T3 (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             2160..3530
                     /codon_start=1
                     /product="bFMO"
                     /label=bFMO
                     /protein_id="ARG47684.1"
                     /translation="MATRIAILGAGPSGMAQLRAFQSAQEKGAEIPELVCFEKQADWGG
                     QWNYTWRTGLDENGEPVHSSMYRYLWSNGPKECLEFADYTFDEHFGKPIASYPPREVLW
                     DYIKGRVEKAGVRKYIRFNTAVRHVEFNEDSQTFTVTVQDHTTDTIYSEEFDYVVCCTG
                     HFSTPYVPEFEGFEKFGGRILHAHDFRDALEFKDKTVLLVGSSYSAEDIGSQCYKYGAK
                     KLISCYRTAPMGYKWPENWDERPNLVRVDTENAYFADGSSEKVDAIILCTGYIHHFPFL
                     NDDLRLVTNNRLWPLNLYKGVVWEDNPKFFYIGMQDQWYSFNMFDAQAWYARDVIMGRL
                     PLPSKEEMKADSMAWREKELTLVTAEEMYTYQGDYIQNLIDMTDYPSFDIPATNKTFLE
                     WKHHKKENIMTFRDHSYRSLMTGTMAPKHHTPWIDALDDSLEAYLSDKSEIPVAKEA"
     misc_feature    3549..3698
                     /label=Terminator DT5
                     /note="Terminator DT5"
     terminator      complement(3755..3802)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3809..3841)
                     /label=ssrA tag (LVA)
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
     misc_feature    complement(3848..3865)
                     /label=similar to SuMMV cleavage site
                     /note="similar to SuMMV cleavage site"
     CDS             complement(3887..6931)
                     /label=lacZ
                     /note="beta-galactosidase"
     regulatory      complement(6972..6994)
                     /label=Promoter CGG (T7 RNAP)
                     /note="Promoter CGG (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             complement(7656..8603)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(8651..8873)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(9365..9459)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9493..10350)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 taataactat cactataggg agacactaca atcataacaa agaagcccgt atgttacgtc
       61 ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg
      121 atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg
      181 caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt cgtaattatg
      241 cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca ggccagcgta
      301 tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat aatcaggaag
      361 tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg tatgttattg
      421 ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg cagactatcc
      481 cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt
      541 tctttaacta tgccgggatc catcgcagcg taatgctcta caccacgccg aacacctggg
      601 tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg tctgttgact
      661 ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg
      721 ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac ctctggcaac
      781 cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca gagtgtgata
      841 tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag ttcctgatta
      901 accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac ttgcgtggca
      961 aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg attggggcca
     1021 actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg gcagatgaac
     1081 atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct ttaggcattg
     1141 gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc aacggggaaa
     1201 ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa aaccacccaa
     1261 gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt gcacgggaat
     1321 atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg
     1381 tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt gatgtgctgt
     1441 gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg gcagagaagg
     1501 tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt atcatcaccg
     1561 aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg tggagtgaag
     1621 agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg
     1681 tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg
     1741 gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc
     1801 tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaat
     1861 gataataatc tagagagctc gacttagata aatatgacat actcggtacc aaattccaga
     1921 aaagaggcct cccgaaaggg gggccttttt tcgttttggt cctccttggc cctccatcct
     1981 tagatagtcc ggcaattaaa aaagcggcta accacgccgc tttttttacg tctgcatcat
     2041 aggcaatacg atcgcatgtc cccgccgccc tagaccggcg tatcacgagg ccctttcgtc
     2101 ttcacctcga gtaataaccc tcactatagg tactagagtc aatcagacag gaggtaataa
     2161 tggcgacccg tattgcaatt ctgggcgcag gcccatcggg tatggcgcaa ttgcgtgcgt
     2221 ttcaaagcgc acaagagaaa ggcgctgaga tcccggagtt ggtttgtttt gagaaacagg
     2281 cggactgggg tggccagtgg aactatactt ggcgtaccgg tctggacgaa aacggcgaac
     2341 cggtccacag ctccatgtac cgttacctgt ggtccaacgg tccgaaagaa tgtttggagt
     2401 ttgctgatta cacctttgat gaacactttg gtaagccaat tgccagctac ccaccgcgtg
     2461 aagtgctgtg ggactatatc aagggtcgcg tggaaaaggc gggtgtccgc aaatacatcc
     2521 gtttcaatac cgcggttcgt catgttgagt tcaatgagga ttctcagacc tttactgtga
     2581 cggttcagga ccataccact gacaccatct atagcgaaga atttgactat gtggtttgct
     2641 gtaccggtca cttcagcacc ccgtatgtcc cggagttcga aggcttcgaa aagttcggtg
     2701 gtcgtattct gcatgcccac gactttcgtg atgcgctgga gttcaaggat aagaccgttc
     2761 tgttggtggg cagctcgtac tctgcggaag atattggcag ccagtgctac aagtatggcg
     2821 cgaagaaact gattagctgc tatcgcaccg caccgatggg ttacaaatgg ccggagaact
     2881 gggacgagcg tccgaacctg gtgcgtgtgg ataccgagaa tgcttacttc gcagatggtt
     2941 cttcggagaa agttgatgcc atcatcctgt gcaccggtta catccaccac ttcccgtttc
     3001 tgaatgacga cttgcgcctg gtgaccaaca atcgcctgtg gccgctgaac ctgtacaagg
     3061 gcgttgtttg ggaggataat ccgaagttct tctacattgg tatgcaagac caatggtaca
     3121 gcttcaacat gttcgatgcc caagcttggt atgcgcgtga tgtgatcatg ggccgtttgc
     3181 cgttgccgag caaagaagaa atgaaggccg acagcatggc gtggcgcgag aaagagctga
     3241 cgctggtcac ggctgaagag atgtatacct accagggtga ctatatccag aacctgatcg
     3301 acatgaccga ttatccgagc tttgatattc cggcgacgaa caaaacgttc ctggaatgga
     3361 aacatcataa gaaagagaac atcatgacgt ttcgcgacca cagctatcgc tccctgatga
     3421 ccggcacgat ggctccaaag caccataccc cgtggatcga tgctctggac gacagcctgg
     3481 aggcttacct gagcgacaag tccgaaatcc cggtggcaaa agaggcctaa taaggatctc
     3541 cggcaattaa aaaagcggct aaccacgccg ctttttttac gtctgcatcc ttggccctcc
     3601 atccttagat agctcggtac caaattccag aaaagaggcc tcccgaaagg ggggcctttt
     3661 ttcgttttgg tcctcatagg caatacgatc gcatgtccct gttgtttgtc ggtgaacgct
     3721 ctcctgagta ggactagggt ccgacaaaca acagcaaaaa acccctcaag acccgtttag
     3781 aggccccaag gggttatgct agttattagg ctaccagggc atagttttcg tcgttcgctg
     3841 cgccaccctg caagtggatt tcctcggatc cgactcgagc ggccgctttt tgacaccaga
     3901 ccaactggta atggtagcga ccggcgctca gctggaattc cgccgatact gacgggctcc
     3961 aggagtcgtc gccaccaatc cccatatgga aaccgtcgat attcagccat gtgccttctt
     4021 ccgcgtgcag cagatggcga tggctggttt ccatcagttg ctgttgactg tagcggctga
     4081 tgttgaactg gaagtcgccg cgccactggt gtgggccata attcaattcg cgcgtcccgc
     4141 agcgcagacc gttttcgctc gggaagacgt acggggtata catgtctgac aatggcagat
     4201 cccagcggtc aaaacaggcg gcagtaaggc ggtcgggata gttttcttgc ggccctaatc
     4261 cgagccagtt tacccgctct gctacctgcg ccagctggca gttcaggcca atccgcgccg
     4321 gatgcggtgt atcgctcgcc acttcaacat caacggtaat cgccatttga ccactaccat
     4381 caatccggta ggttttccgg ctgataaata aggttttccc ctgatgctgc cacgcgtgag
     4441 cggtcgtaat cagcaccgca tcagcaagtg tatctgccgt gcactgcaac aacgctgctt
     4501 cggcctggta atggcccgcc gccttccagc gttcgaccca ggcgttaggg tcaatgcggg
     4561 tcgcttcact tacgccaatg tcgttatcca gcggtgcacg ggtgaactga tcgcgcagcg
     4621 gcgtcagcag ttggttttta tcgccaatcc acatctgtga aagagagcct gactggcggt
     4681 taaattgcca acgcttatta cccagctcga tgcaaaaatc catttcgctg gtggtcagat
     4741 gcgggatggc gtgggacgcg gcggggagcg tcacactgag gttttccgcc agacgccact
     4801 gctgccaggc gctgatgtgc ccggcttctg accatgcggt cgcgttcggt tgcactacgc
     4861 gtactgtgag ccagagttgc ccggcgctct ccggctgcgg tagttcaggc agttcaatca
     4921 actgtttacc ttgtggagcg acatccagag gcacttcacc gcttgccagc ggcttaccat
     4981 ccagcgccac catccagtgc aggagctcgt tatcgctatg acggaacagg tattcgctgg
     5041 tcacttcgat ggtttgcccg gataaacgga actggaaaaa ctgctgctgg tgttttgctt
     5101 ccgtcagcgc tggatgcggc gtgcggtcgg caaagaccag accgttcata cagaactggc
     5161 gatcgttcgg cgtatcgcca aaatcaccgc cgtaagccga ccacgggttg ccgttttcat
     5221 catatttaat cagcgactga tccacccagt cccagacgaa gccgccctgt aaacggggat
     5281 actgacgaaa cgcctgccag tatttagcga aaccgccaag actgttaccc atcgcgtggg
     5341 cgtattcgca aaggatcagc gggcgcgtct ctccaggtag cgaaagccat tttttgatgg
     5401 accatttcgg cacagccggg aagggctggt cttcatccac gcgcgcgtac atcgggcaaa
     5461 taatatcggt ggccgtggtg tcggctccgc cgccttcata ctgcaccggg cgggaaggat
     5521 cgacagattt gatccagcga tacagcgcgt cgtgattagc gccgtggcct gattcattcc
     5581 ccagcgacca gatgatcaca ctcgggtgat tacgatcgcg ctgcaccatt cgcgttacgc
     5641 gttcgctcat cgccggtagc cagcgcggat catcggtcag acgattcatt ggcaccatgc
     5701 cgtgggtttc aatattggct tcatccacca catacaggcc gtagcggtcg cacagcgtgt
     5761 accacagcgg atggttcgga taatgcgaac agcgcacggc gttaaagttg ttctgcttca
     5821 tcagcaggat atcctgcacc atcgtctgct catccatgac ctgaccatgc agaggatgat
     5881 gctcgtgacg gttaacgcct cgaatcagca acggcttgcc gttcagcagc agcagaccat
     5941 tttcaatccg cacctcgcgg aaaccgacat cgcaggcttc tgcttcaatc agcgtgccgt
     6001 cggcggtgtg cagttcaacc accgcacgat agagattcgg gatttcggcg ctccacagtt
     6061 tcgggttttc gacgttcaga cgtagtgtga cgcgatcggc ataaccacca cgctcatcga
     6121 taatttcacc gccgaaaggc gcggtgccgc tggcgacctg cgtttcaccc tgccataaag
     6181 aaactgttac ccgtaggtag tcacgcaact cgccgcacat ctgaacttca gcctccagta
     6241 cagcgcggct gaaatcatca ttaaagcgag tggcaacatg gaaatcgctg atttgtgtag
     6301 tcggtttatg cagcaacgag acgtcacgga aaatgccgct catccgccac atatcctgat
     6361 cttccagata actgccgtca ctccagcgca gcaccatcac cgcgaggcgg ttttctccgg
     6421 cgcgtaaaaa tgcgctcagg tcaaattcag acggcaaacg actgtcctgg ccgtaaccga
     6481 cccagcgccc gttgcaccac agatgaaacg ccgagttaac gccatcaaaa ataattcgcg
     6541 tctggccttc ctgtagccag ctttcatcaa cattaaatgt gagcgagtaa caacccgtcg
     6601 gattctccgt gggaacaaac ggcggattga ccgtaatggg ataggtcacg ttggtgtaga
     6661 tgggcgcatc gtaaccgtgc atctgccagt ttgaggggac gacgacagta tcggcctcag
     6721 gaagatcgca ctccagccag ctttccggca ccgcttctgg tgccggaaac caggcaaagc
     6781 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6841 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     6901 ggttttccca gtcacgacgt tgtaaaacga cgggatctat catgtctatt agtcttcctc
     6961 tttcttcctt atctccctat agtgaccggt attactcgag gtgaagacga aagggcctcg
     7021 tgatacgcca cctcgtattg cccagagtgc ggtatctaga caaaaaaccc cttcgccccc
     7081 gttagcggtt ctgctattat ttaagctggg taaatgcaga taatcgtttt ctggcttcga
     7141 cgggttttgc tgcccgcaaa cgggctgttc tggtgttgct agtttgttat cagaatcgca
     7201 gatccggctt caggtttgcc ggctgaaagc gctatttctt ccagaattgc catgattttt
     7261 tccccacggg aggcgtcact ggctcccgtg ttgtcggcag ctttgattcg ataagcagca
     7321 tcgcctgttt caggctgtct atgtgtgact gttgagctgt aacaagttgt ctcaggtgtt
     7381 caatttcatg ttctagttgc tttgttttac tggtttcacc tgttctatta ggtgttacat
     7441 gctgttcatc tgttacattg tcgatctgtt catggtgaac agctttaaat gcaccaaaaa
     7501 ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt ttcatctgtg catatggaca
     7561 gttttccctt tgatatctaa cggtgaacag ttgttctact tttgtttgtt agtcttgatg
     7621 cttcactgat agatacaaga gccataagaa cctcagatcc ttccgtattt agccagtatg
     7681 ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt gagatcatgc
     7741 ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt
     7801 aaagcatcgt gtagtgtttt tcttagtccg ttacgtaggt aggaatctga tgtaatggtt
     7861 gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt tacgagatcc
     7921 atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca
     7981 accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt caaaacccat
     8041 tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa cttaaattca
     8101 tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc ttttaataac
     8161 cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc cagattatat
     8221 tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa aactaattct
     8281 aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa gcctttaacc
     8341 aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt agctaataca
     8401 ccataagcat tttccctact gatgttcatc atctgagcgt attggttata agtgaacgat
     8461 accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc cacacagcat
     8521 aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag ttcatttgct
     8581 ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta atcactatac
     8641 caattgagat gggctagtca atgataatta ctagtccttt tcctttgagt tgtgggtatc
     8701 tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat
     8761 tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat ttatagaata
     8821 aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa ctcactactt
     8881 tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac
     8941 agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa atcgctgaat
     9001 attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt
     9061 cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc gccttttatg
     9121 gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct tctcagggcg
     9181 ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc
     9241 ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta
     9301 atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg tccctagtgc
     9361 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     9421 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc tcgaacccca
     9481 gagtcccgct taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9541 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9601 atctggcccc agtgctgcaa tgataccgcg gctgccacgc tcaccggctc cagatttatc
     9661 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9721 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9781 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9841 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9901 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9961 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10021 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10081 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10141 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10201 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10261 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10321 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10381 ttatcgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
    10441 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
    10501 ggcgccccag ctggcaattc cgacgtctaa gaaaccatta ttatcatgac attaacctat
    10561 aaaaata
//
