LOCUS       40924_26026        5846 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJFR2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Multichromatic Bacterial Photography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Biological Engineering, MIT, 500 Tech 
            Square, Rm 140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 5846)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5846)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2016) Biological Engineering, MIT, 500 Tech Square, Rm 140, 
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      46..292
                     /label=Promoter FixK2
                     /note="Promoter FixK2"
                     /regulatory_class="promoter"
     CDS             326..928
                     /codon_start=1
                     /product="PhlF"
                     /label=PhlF
                     /protein_id="ARG47674.1"
                     /translation="MARTPSRSSIGSLRSPHTHKAILTSTIEILKECGYSGLSIESVAR
                     RAGASKPTIYRWWTNKAALIAEVYENESEQVRKFPDLGSFKADLDFLLRNLWKVWRETI
                     CGEAFRCVIAEAQLDPATLTQLKDQFMERRREMPKKLVENAISNGELPKDTNRELLLDM
                     IFGFCWYRLLTEQLTVEQDIEEFTFLLINGVCPGTQR"
     terminator      952..1023
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1039..1066
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1109..1138
                     /label=Operator PhlF
                     /note="Operator PhlF"
     misc_RNA        1146..1196
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     misc_feature    1225..1350
                     /label=similar to SynZip18
                     /note="similar to SynZip18"
     misc_feature    1351..1365
                     /label=similar to Linker GGSGG
                     /note="similar to Linker GGSGG"
     misc_feature    1366..2220
                     /label=similar to T3 fragment of T7 RNAP
                     /note="similar to T3 fragment of T7 RNAP"
     misc_feature    2230..2276
                     /label=Terminator L3S3P11
                     /note="Terminator L3S3P11"
     misc_feature    2342..2485
                     /label=Terminator DT25
                     /note="Terminator DT25"
     misc_feature    complement(2542..3396)
                     /label=similar to CGG fragment of T7 RNAP
                     /note="similar to CGG fragment of T7 RNAP"
     misc_feature    complement(3397..3411)
                     /label=similar to Linker GGSGG
                     /note="similar to Linker GGSGG"
     misc_feature    3412..3537
                     /label=similar to SynZip 18
                     /note="similar to SynZip 18"
     misc_feature    3579..3629
                     /label=Insulator BydvJ
                     /note="Insulator BydvJ"
     regulatory      3632..3803
                     /label=Promoter PcpcG2
                     /note="Promoter PcpcG2"
                     /regulatory_class="promoter"
     terminator      3943..4029
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(4198..4743)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    4849..4899
                     /label=Terminator L3S1P13
                     /note="Terminator L3S1P13"
     CDS             complement(4910..5698)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      5699..5846
                     /regulatory_class="promoter"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccacgcc cgtgatcctg
       61 atcaccggct atccggacga aaacatctcg acccgggccg ccgaggccgg cgtaaaagac
      121 gtggttttga agccgcttct cgacgaaaac ctgctcaagc gtatccgccg cgccatccag
      181 gaccggcctc gggcatgacc tacggggttc tacgtaaggc acccccctta agatatcgct
      241 cgaaattttc gaacctcccg ataccgcgta ccaatgcgtc atcacaacgg agctatggac
      301 tatgtttaaa aaacgagaat actatatggc acgtaccccg agccgtagca gcattggtag
      361 cctgcgtagt ccgcataccc ataaagcaat tctgaccagc accattgaaa tcctgaaaga
      421 atgtggttat agcggtctga gcattgaaag cgttgcacgt cgtgccggtg caagcaaacc
      481 gaccatttat cgttggtgga ccaataaagc agcactgatt gccgaagtgt atgaaaatga
      541 aagcgaacag gtgcgtaaat ttccggatct gggtagcttt aaagccgatc tggattttct
      601 gctgcgtaat ctgtggaaag tttggcgtga aaccatttgt ggtgaagcat ttcgttgtgt
      661 tattgcagaa gcacagctgg accctgcaac cctgacccag ctgaaagatc agtttatgga
      721 acgtcgtcgt gagatgccga aaaaactggt tgaaaatgcc attagcaatg gtgaactgcc
      781 gaaagatacc aatcgtgaac tgctgctgga tatgattttt ggtttttgtt ggtatcgcct
      841 gctgaccgaa cagctgaccg ttgaacagga tattgaagaa tttaccttcc tgctgattaa
      901 tggtgtttgt ccgggtacac agcgttaact agggcccata cccccaggca tcaaataaaa
      961 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1021 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacgacgtac
     1081 ggtggaatct gattcgttac caattgacat gatacgaaac gtaccgtatc gttaaggtta
     1141 ctagagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc
     1201 tacaaataat tttgtttaaa ctatatgagc atcgcggcga ccctggagaa cgatctggcg
     1261 cgtctggaaa acgaaaacgc tcgtctcgaa aaagacatcg cgaacctgga acgtgacctg
     1321 gcgaaactgg agcgtgaaga agcgtacttc ggaggttcag gtggtaagaa cactggtgaa
     1381 atctctgaga aagtcaagct gggcactaag gcactggctg gtcaatggct ggcttacggt
     1441 gttactcgca gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc caaagagttc
     1501 ggcttccgtc aacaagtgct ggaagatacc attcagccag ctattgattc cggcaagggt
     1561 ctgatgttca ctcagccgaa tcaggctgct ggatacatgg ctaagctgat ttgggaatct
     1621 gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag
     1681 ctgctggctg ctgaggtcaa agataagaag actggagaga ttcttcgcaa gcgttgcgct
     1741 gtgcattggg taactcctga tggtttccct gtgtggcagg aatacaagaa gcctattcag
     1801 aagcgcctgg acatgatttt cttgggtcaa tttcgcttgc aacctaccat taacaccaac
     1861 aaagatagcg agattgatgc acacaaacag gagtctggta tcgctcctaa ctttgtacac
     1921 agccaagacg gtagccacct tcgtaagact gtagtgtggg cacacgagaa gtacggaatc
     1981 gaatcttttg cactgattca cgactccttc ggtacgattc cggctgacgc tgcgaacctg
     2041 ttcaaagcag tgcgcgaaac tatggttgac acatatgagt cttgtgatgt actggctgat
     2101 ttctacgacc agttcgctga ccagttgcac gagtctcaat tggacaaaat gccagcactt
     2161 ccggctaaag gtaacttgaa cctccgtgac atcttagagt cggacttcgc gttcgcgtaa
     2221 taatctagac caattattga acacccttcg gggtgttttt ttgtttctgg tctcccccta
     2281 ggcgttctct agataaaagc cgtagggcgc cgcagccgcc gtaggcggtc acgcagatga
     2341 cggacatgcg atcgtattgc ctatgaccac caatgtaaaa aagcgcccta aaggcgcttt
     2401 tttgctatct gcgaatgttc gtcttttcta ttcagtcacc tttggtcgaa aaaaaaagcc
     2461 cgcactgtca ggtgcgggct tttttctgtg tttcccattt gaccacgcag ccacataccc
     2521 ctggacgagc tctctagatt attaggcgaa cgcgaaatcg ctttccagga tgtcacgcag
     2581 gttaaggttg ccttttgccg gcagcgccgg cattttatcc agctggcttt catgcagctg
     2641 gtccgcaaac tgatcataaa aatctgccag cacatcgcag ctttcgtagg tatcaaccat
     2701 ggtttcacga acggctttga acaggttcgc tgcgtcggct gggatagtac cgaaggaatc
     2761 gtgaatcagt gcaaaagact cgatgccgta tttttcgtgg gcccatacca cggttttacg
     2821 cagatgggaa ccgtcctggc tatgcacaaa gttcggagcg atgccagatt catgcttacg
     2881 agcgtcgatt tcggaatctt tgttagtatt aatagtcggc tgcatttcga actgacccag
     2941 aaacatgata tgaacgcgag ttttgatagg ttttttatat tcctgccaaa ccggaaaacc
     3001 gtccggggtt acccagtgga cagcgcaacg tttacgcagg atctcgccag tttttttatc
     3061 tttaacttca gcagccagca acttcgctgc ggactttaac cagttcatag cttcaactgc
     3121 agcaacaacg gtgactgaaa cgctttccca gatcagcttc gccatatagc ctgctgcctg
     3181 gttcggctgg gtgaacatca ggcctttgcc agagtcgatt gccggctgaa tggtgtcttc
     3241 cagaacctgc tgacggaagc cgaattcttt agacccatat gcgagggtca ttacggaacg
     3301 cttggtgaca gaacgggtca cgccatacgc cagccactga ccagccagtg ccttggtacc
     3361 cagtttcact ttctcggaaa tttcgccagt gtttttaccg ccggagccac cgaagtaggc
     3421 ctcttcacgt tccagttttg ccagatcgcg ttccaggttg gcaatatctt tttccagacg
     3481 cgcattttcg ttttccagac gagccaagtc gttttccaaa gtagcggcaa tcgacatctc
     3541 gtcttcctct tttttttaaa caaaattatt tgtagagggg tgtttcgtcc tttcggactc
     3601 atcagtcaag gtacgcacct tgagacaccc tttaaaaatg cgatcctaac aaagtaaaat
     3661 tgaagaaaag ggggagaaaa tcgtaaagaa atcggaaagt tagctgatgc gactaaaact
     3721 gttacatttc gcaacatgaa ttaacccaga cctgttcacc ccttcaggta agctgaggtt
     3781 gatagagaaa agcacaatgg gcttgactag ctaactaaca gtatagattt tgtcagcctt
     3841 gaattccagc acactggcgg ccgttactag tggatccaat aagccgaatt ctgcagatat
     3901 ccatcacact ggcggccgct taattaatta atctagaggc atcaaataaa acgaaaggct
     3961 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     4021 aggacaaatc cgccgcccta gacctaggga tatattccgc ttcctcgctc actgactcgc
     4081 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
     4141 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
     4201 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     4261 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
     4321 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     4381 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     4441 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4501 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta
     4561 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct
     4621 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg
     4681 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct
     4741 caagaagatc atcttattaa tcagataaaa tattactaga tttcagtgca atttatctct
     4801 tcaaatgtag cacctgaagt cagccccata cgatataagt tgttactatt ttgttatcaa
     4861 taaaaaaggc cccccgttag ggaggcctta ttgttcgtcc gagctcttat ttgccgacta
     4921 ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg
     4981 ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat
     5041 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
     5101 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
     5161 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
     5221 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
     5281 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
     5341 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
     5401 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
     5461 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
     5521 catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc
     5581 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
     5641 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatgc
     5701 gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat cagatccttg
     5761 gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc
     5821 cagctggcaa ttccggttcg cttgct
//
