LOCUS       V015653                 6550 bp    DNA     circular SYN 01-FEB-2022
DEFINITION  Exported.
ACCESSION   V015653
VERSION     V015653
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Ruf S, Forner J, Hasse C, Kroop X, Seeger S, Schollbach L, Schadach
            A, Bock R.
  TITLE     High-efficiency generation of fertile transplastomic Arabidopsis
            plants
  JOURNAL   Nat Plants 5 (3), 282-289 (2019)
   PUBMED   30778165
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Forner J, Hasse C, Ruf S, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2018) Organelle Biology, Biotechnology and
            Molecular Ecophysiology, Max Planck Institute of Molecular Plant
            Physiology, Am Muehlenberg 1, Potsdam, Brandenburg D-14476, Germany
REFERENCE   3  (bases 1 to 6550)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Plants"; date: "2019"; volume: "5"; issue: "3"; pages: "282-289"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-JUL-2018) Organelle Biology, Biotechnology and Molecular
            Ecophysiology, Max Planck Institute of Molecular Plant Physiology,
            Am Muehlenberg 1, Potsdam, Brandenburg D-14476, Germany"
FEATURES             Location/Qualifiers
     source          1..6550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..521
                     /gene="psaB"
                     /label="psaB"
     CDS             1..521
                     /codon_start=3
                     /transl_table=11
                     /gene="psaB"
                     /product="PSAB"
                     /label="psaB"
                     /protein_id="QBG49749.1"
                     /translation="GRGGTCDISAWDAFYLAVFWMLNTIGWVTFYWHWKHITLWQGNVS
                     QFNESSTYLMGWLRDYLWLNSSQLINGYNPFGMNSLSVWAWMFLFGHLVWATGFMFLIS
                     WRGYWQELIETLAWAHERTPLANLIRWKDKPVALSIVQARLVGLAHFSVGYIFTYAAFL
                     IASTSGKFG"
     CDS             656..955
                     /gene="rps14"
                     /label="Small ribosomal subunit protein uS14c"
                     /note="Small ribosomal subunit protein uS14c from
                     Arabidopsis thaliana. Accession#: P56804"
     tRNA            1119..1192
                     /product="tRNA-Met"
                     /label="trnfM"
                     /note="trnfM"
     regulatory      1302..1451
                     /label="Zea mays clpP promoter"
                     /note="Zea mays clpP promoter"
                     /regulatory_class="promoter"
     CDS             1453..2241
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     terminator      2252..2338
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2430..2457
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     primer_bind     complement(2477..2493)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     tRNA            complement(2553..2623)
                     /product="tRNA-Gly"
     CDS             complement(3177..3362)
                     /gene="psbZ"
                     /label="Photosystem II reaction center protein Z"
                     /note="Photosystem II reaction center protein Z from
                     Nicotiana tabacum. Accession#: P09974"
     promoter        complement(3701..3719)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3729..3745)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3887..4342
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4368..4472
                     /label="AmpR promoter"
     CDS             4473..5330
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5504..6092
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6380..6401
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6416..6446
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6454..6470
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6478..6494
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6515..6533
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cgggacgagg tggtacttgt gatatttcgg cttgggacgc attttatttg gcagttttct
       61 ggatgttaaa tactattgga tgggttactt tttattggca ttggaaacac atcacattat
      121 ggcagggtaa cgtttcacag tttaatgaat cttccactta tttgatggga tggttaagag
      181 attatctatg gttaaactct tcacaactta tcaatggata taacccgttt ggtatgaata
      241 gtttatcagt ctgggcatgg atgttcttat ttgggcatct tgtttgggct acaggattta
      301 tgttcttaat ttcctggcgt ggttattggc aggaattgat tgaaacttta gcgtgggctc
      361 atgaacgcac acctttggca aatttgattc gctggaaaga taaaccagta gctctttcaa
      421 tcgtgcaagc aagattggtt ggattagctc acttttctgt aggttatata ttcacttatg
      481 cggctttctt gattgcctcc acatcgggca aattcggtta attatttatg tattctatcc
      541 gcaatcatat atttttttta ataaaaaaaa tataggtatg cactcttata tttatttcta
      601 catctaggat ccgatttgta tcattatcat tgataataac aggaactgaa gcattatggc
      661 aaagaaaagt ttgatttata gggagaagaa gaggcaaaaa ttggaaaaaa aatatcattt
      721 gattcgtcga tcctcaaaaa aggaaataag taagattccg tcgctaagtg agaaatggaa
      781 aattcatgga aaattacaat ccccaccgcg taatagtgca cctacacgtc ttcatcgacg
      841 ttgcttttcg accggaagac cgagagctaa ctatcgagac tttggactat ctggacacat
      901 ccttcgggaa atggttcagg catgtttgtt gccaggggca acaagatcaa gctggtaagg
      961 atttaagtat cccttaattt ttctattttt ttctatgatc gatgaggccc ctttaccatt
     1021 ctgtctaaat aggctattct atttgtacag atatggaaga ggggctcctt caattattct
     1081 ttgtttatta gagtttaatc caagtttttt tgtttcagcg cggggtagag cagtttggta
     1141 gctcgcaagg ctcataacct tgaggtcacg ggttcaaatc ctgtctccgc aacttttttt
     1201 tttcaagaaa tgtaagtttg attctactaa caagtcataa attaatacgt gtcttttggg
     1261 acgcgaggaa aaaattacaa tatttcccgg agctcggatc ctctatgtat taatagaatc
     1321 tatagtattc ttatagaata agaaaaaaaa aatgaagata ataaactgcg gattctttct
     1381 ttctcttcca ttcttacgtt tccatattaa agtgtagttt ttttacttaa atttaaagaa
     1441 ggagatatac ccatggaaga agctgttatt gctgaagttt ctacacaatt atctgaagtt
     1501 gttggagtta ttgaaagaca tttagaacct acattattag ctgttcattt atatggatct
     1561 gctgttgatg gaggattaaa acctcattct gatattgatt tattagttac tgttacagtt
     1621 agattagatg aaactacaag aagagcttta attaatgatt tattagaaac ttctgcttct
     1681 cctggagaat ctgaaatttt aagagctgtt gaagttacta ttgttgttca tgatgatatt
     1741 attccttgga gatatcctgc taaaagagaa ttacaatttg gagaatggca aagaaatgat
     1801 attttagctg gaatttttga acctgctact attgatattg atttagctat tttattaaca
     1861 aaagctagag aacattctgt tgctttagtt ggacctgctg ctgaagaatt atttgatcct
     1921 gttccggaac aagatttatt tgaagcatta aatgaaacat taactttatg gaactctcct
     1981 cctgattggg ctggagatga aagaaatgtt gttttaacat tatccagaat ttggtattct
     2041 gctgttacag gaaaaattgc tcctaaagat gttgctgctg attgggctat ggaaagatta
     2101 cctgctcaat atcaacctgt tattttagaa gctagacagg cttatttagg acaagaagaa
     2161 gatagattag cttcaagagc tgatcaatta gaagaatttg ttcattatgt taaaggagaa
     2221 attacaaaag ttgttggaaa ataatctaga tcaaataaaa cgaaaggctc agtcgaaaga
     2281 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     2341 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     2401 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
     2461 gtttctacaa gcttatcgat accgtcgacc tcgagggggg gcccggtaca actataccga
     2521 atattttctc tttttgaatc catttatatt tgggcggata gcgggaatcg aacccgcgtc
     2581 ttctccttgg caaggagaaa ttttaccatt cgactatatc cgcatttttt gcccttcttt
     2641 ataagaaatt tatggataat atttgttcaa agctagacga gatacactct ttggtttggg
     2701 gtcatttcat aaaattctac caccaaataa attcaattaa caattctatt aatatatata
     2761 tatatattat aaatatatat attaataata tatattatat ttattctata tattaatata
     2821 ttaatattaa ttcttgaata ttaatttaat ttaatattga attccagatt caagaatata
     2881 ttattcttat atttccataa aatattatat tctatattga cttgatatca gatctcaatt
     2941 ttttattcgt tttttttatt tatttttttt tattactatt tcatatttag gaactatttc
     3001 atatttagta actatttatt aatcttttat tcatatttca ttcaagttcc ataagttcga
     3061 ccccccctct aattttttct ttatttgcat tttatggact tatattgaat ttgaatgtgt
     3121 aattcaagga atgggagggg gtcatctcat ttttggatct gcaacgaatg aattcaagag
     3181 ataagagaat taaggatacc caccaagaag actaatccaa tccataaaga tgtaccagaa
     3241 aatacaacat ttttgttact cgaccaacca tcaggagacg caaatacaac gggtacacta
     3301 atcagtaaga ttgatgaagt aataattaat gcaaaaactg ccaattggaa agcaatagtc
     3361 atgtttttaa tcctccaagc tattaacaaa agaatataca ccatttaatc ctcttaccca
     3421 ctaaaaattt ttaataaata aagggatttt atcatgaatc cgttgattcg agatgactta
     3481 gttccaattt ctttttacca cttttagtct attcggacat gaagttaaag gttctttttt
     3541 acttcacaaa cgggtatact ggatcgcgta tctcatttat ttatatagcc agatttatag
     3601 acctattaaa gaaagtcaca ccctatatct ttctctacat agtgatcgta ttaactcata
     3661 gggctatgtt ctatttggcg aaggtacgta cccaattcgc cctatagtga gtcgtattac
     3721 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3781 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3841 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     3901 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3961 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4021 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4081 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4141 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4201 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4261 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4321 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4381 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4441 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4501 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4561 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4621 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4681 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4741 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4801 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4861 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4921 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4981 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5041 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5101 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5161 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5221 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5281 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5341 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5401 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5461 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5521 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5581 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5641 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5701 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5761 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5821 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5881 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5941 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6001 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6061 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6121 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6181 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6241 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6301 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6361 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6421 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6481 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     6541 agctggagat
//
